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The map label for this gene is gltA [H]

Identifier: 189352638

GI number: 189352638

Start: 725375

End: 726676

Strand: Direct

Name: gltA [H]

Synonym: BMULJ_03869

Alternate gene names: 189352638

Gene position: 725375-726676 (Clockwise)

Preceding gene: 189352637

Following gene: 189352640

Centisome position: 29.33

GC content: 61.14

Gene sequence:

>1302_bases
ATGACTCCGTCTGATGTTAAAGCCACGCTATCGTTCAGCGACAATTCGCCGAGCGTCGAACTGCCGATCTACAAGGGCAC
GATGGGCCCGGACGTGATCGACATCCGCAAGCTGTACGGCCAGACCGGCAAGTTCACGTACGACCCGGGCTTCATGTCGA
CGGCGTCGTGCAATTCGGCGATCACGTACATCGACGGCGACAAGGGCGAGCTGCTGTATCGCGGCTACCCGATCGACAAC
CTCGCGCAGAACGCCGACTTCCTCGAAACCTGCTATCTGCTGCTGAAGGGCGAGCTGCCGAACGCAGCGCAGAAGAAGGA
ATTCGTCGACACCGTCACGAAGCACACGATGGTGCACGAGCAGATGCACTTCTTCTTCCGCGGCTTCCGCCGCGATGCGC
ACCCGATGGCGATCCTGGTCGCCGCGGTCGGCGCGCTGTCGGCGTTCTACCACGACTCGCTCGACATCAACGATCCGCGT
CACCGTGAAGTGTCGGCGATCCGCATGATCGCGAAGCTGCCGACGCTCGTCGCGATGGCGTACAAGTACAGCATCGGCCA
GCCGTTCGTCTATCCGAAGAACGATCTGTCGTACAGCGCGAACTTCATGCGGATGATGTTCTCGAATCCGTGCGAAGAGT
ACCAAGTCAACGACGTGCTCGTCCGCGCGCTCGACCGCATCCTGATCCTGCACGCCGATCACGAGCAGAACGCATCGACC
TCGACGGTCCGTCTCGCGGGCTCGTCGGGTGCGAACCCGTTCGCGTGTATCGCAGCCGGTATCGCGTGTCTGTGGGGCCC
GGCGCACGGCGGTGCGAACGAAGCCGCGCTGAACATGCTCGAGCAGATCGGCACGCCGGAAAACATTCCCGAGTTCATCA
AGCAGGTGAAGGACAAGAATTCGGGCGTGAAGCTGATGGGCTTCGGCCACCGCGTGTACAAGAACTACGATCCGCGCGCG
AAACTGATGCGCGAAACGTGCTACGAAGTGCTGAACGAACTCGGCCTGCACGACGATCCGCTGTTCAAGCTCGCGATGCA
GCTCGAAAAGATCGCGCTGGAAGACGAATACTTCGTGTCGCGCAAGCTGTACCCGAACGTCGACTTCTACTCGGGCATCG
TTCAGCGCGCGCTGGGCATCCCGACGTCGATGTTCACCTGCATCTTCGCGATGGCACGTACGGTCGGCTGGATCGCGCAG
TGGAACGAAATGATCGCCGATCCGGAGCAGAAGATCGGTCGTCCGCGTCAGCTGTTCATCGGCGACACGCCGCGCGAAGC
GAAGCCGCTGAACGCACGTTAA

Upstream 100 bases:

>100_bases
ACAGCCCCGACGTTCACCGGCTGTTGGAGATGCTGCGCAACGTGTAACGCGTTACGCCCGTTATCGAATCCCGTTTCTTT
ATTTCGATTGAGGATGTGCC

Downstream 100 bases:

>100_bases
GCCGTGCACGACGCGAGCCGGCCGGCAGGCCGCTCGCGTTCGCCGCATGCCGCACCCCGGTGGGTCATCCCGCCGGGGTG
TTTTCATTTGCGCGGCGTGC

Product: type II citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MTPSDVKATLSFSDNSPSVELPIYKGTMGPDVIDIRKLYGQTGKFTYDPGFMSTASCNSAITYIDGDKGELLYRGYPIDN
LAQNADFLETCYLLLKGELPNAAQKKEFVDTVTKHTMVHEQMHFFFRGFRRDAHPMAILVAAVGALSAFYHDSLDINDPR
HREVSAIRMIAKLPTLVAMAYKYSIGQPFVYPKNDLSYSANFMRMMFSNPCEEYQVNDVLVRALDRILILHADHEQNAST
STVRLAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLEQIGTPENIPEFIKQVKDKNSGVKLMGFGHRVYKNYDPRA
KLMRETCYEVLNELGLHDDPLFKLAMQLEKIALEDEYFVSRKLYPNVDFYSGIVQRALGIPTSMFTCIFAMARTVGWIAQ
WNEMIADPEQKIGRPRQLFIGDTPREAKPLNAR

Sequences:

>Translated_433_residues
MTPSDVKATLSFSDNSPSVELPIYKGTMGPDVIDIRKLYGQTGKFTYDPGFMSTASCNSAITYIDGDKGELLYRGYPIDN
LAQNADFLETCYLLLKGELPNAAQKKEFVDTVTKHTMVHEQMHFFFRGFRRDAHPMAILVAAVGALSAFYHDSLDINDPR
HREVSAIRMIAKLPTLVAMAYKYSIGQPFVYPKNDLSYSANFMRMMFSNPCEEYQVNDVLVRALDRILILHADHEQNAST
STVRLAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLEQIGTPENIPEFIKQVKDKNSGVKLMGFGHRVYKNYDPRA
KLMRETCYEVLNELGLHDDPLFKLAMQLEKIALEDEYFVSRKLYPNVDFYSGIVQRALGIPTSMFTCIFAMARTVGWIAQ
WNEMIADPEQKIGRPRQLFIGDTPREAKPLNAR
>Mature_432_residues
TPSDVKATLSFSDNSPSVELPIYKGTMGPDVIDIRKLYGQTGKFTYDPGFMSTASCNSAITYIDGDKGELLYRGYPIDNL
AQNADFLETCYLLLKGELPNAAQKKEFVDTVTKHTMVHEQMHFFFRGFRRDAHPMAILVAAVGALSAFYHDSLDINDPRH
REVSAIRMIAKLPTLVAMAYKYSIGQPFVYPKNDLSYSANFMRMMFSNPCEEYQVNDVLVRALDRILILHADHEQNASTS
TVRLAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLEQIGTPENIPEFIKQVKDKNSGVKLMGFGHRVYKNYDPRAK
LMRETCYEVLNELGLHDDPLFKLAMQLEKIALEDEYFVSRKLYPNVDFYSGIVQRALGIPTSMFTCIFAMARTVGWIAQW
NEMIADPEQKIGRPRQLFIGDTPREAKPLNAR

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=353, Percent_Identity=29.1784702549575, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1786939, Length=425, Percent_Identity=66.5882352941177, Blast_Score=609, Evalue=1e-176,
Organism=Escherichia coli, GI1786527, Length=384, Percent_Identity=28.6458333333333, Blast_Score=157, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17555174, Length=413, Percent_Identity=27.8450363196126, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6319850, Length=330, Percent_Identity=27.8787878787879, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324328, Length=340, Percent_Identity=26.4705882352941, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6325257, Length=368, Percent_Identity=24.1847826086957, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24640126, Length=424, Percent_Identity=26.8867924528302, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24640124, Length=424, Percent_Identity=26.8867924528302, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21356863, Length=425, Percent_Identity=26.3529411764706, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 48530; Mature: 48399

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPSDVKATLSFSDNSPSVELPIYKGTMGPDVIDIRKLYGQTGKFTYDPGFMSTASCNSA
CCCCCCEEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCEEECCCCCCCCCCCCE
ITYIDGDKGELLYRGYPIDNLAQNADFLETCYLLLKGELPNAAQKKEFVDTVTKHTMVHE
EEEEECCCCCEEEECCCHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
QMHFFFRGFRRDAHPMAILVAAVGALSAFYHDSLDINDPRHREVSAIRMIAKLPTLVAMA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YKYSIGQPFVYPKNDLSYSANFMRMMFSNPCEEYQVNDVLVRALDRILILHADHEQNAST
HHHHCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCC
STVRLAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLEQIGTPENIPEFIKQVKDKN
CEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCC
SGVKLMGFGHRVYKNYDPRAKLMRETCYEVLNELGLHDDPLFKLAMQLEKIALEDEYFVS
CCEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHH
RKLYPNVDFYSGIVQRALGIPTSMFTCIFAMARTVGWIAQWNEMIADPEQKIGRPRQLFI
HCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEE
GDTPREAKPLNAR
CCCCCCCCCCCCC
>Mature Secondary Structure 
TPSDVKATLSFSDNSPSVELPIYKGTMGPDVIDIRKLYGQTGKFTYDPGFMSTASCNSA
CCCCCEEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCEEECCCCCCCCCCCCE
ITYIDGDKGELLYRGYPIDNLAQNADFLETCYLLLKGELPNAAQKKEFVDTVTKHTMVHE
EEEEECCCCCEEEECCCHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
QMHFFFRGFRRDAHPMAILVAAVGALSAFYHDSLDINDPRHREVSAIRMIAKLPTLVAMA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YKYSIGQPFVYPKNDLSYSANFMRMMFSNPCEEYQVNDVLVRALDRILILHADHEQNAST
HHHHCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCC
STVRLAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLEQIGTPENIPEFIKQVKDKN
CEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCC
SGVKLMGFGHRVYKNYDPRAKLMRETCYEVLNELGLHDDPLFKLAMQLEKIALEDEYFVS
CCEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHH
RKLYPNVDFYSGIVQRALGIPTSMFTCIFAMARTVGWIAQWNEMIADPEQKIGRPRQLFI
HCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEE
GDTPREAKPLNAR
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]