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The map label for this gene is potB [H]

Identifier: 189352620

GI number: 189352620

Start: 705594

End: 706859

Strand: Direct

Name: potB [H]

Synonym: BMULJ_03851

Alternate gene names: 189352620

Gene position: 705594-706859 (Clockwise)

Preceding gene: 189352619

Following gene: 189352621

Centisome position: 28.53

GC content: 66.98

Gene sequence:

>1266_bases
TTGAATACGATGACGATCGCTTCTTCCCCGACGTCGACGGCCGCGCTCAAGCGCGAGCTGAAGGCGGCCGAGGCCCGCAA
GCGCACGATGGCGCTGCTGCTGATCGCACCGCTCGCGATCTTCCTGCTGCTGGTGTTCGTCGTGCCGATCGGCACGCTGC
TCACGCGCGCGGTGCAGAACCCGGAGATCGCCGCCGCGTTGCCGAACACGGTCGCCGCGCTGTCGCACTGGGACCGCAAG
ACGCCGCCCTCCGACGCCGCCTACGCGGCGCTCGCCACCGACATGACGAAAGTCGCCGACAGCGACGCGATGGGCGCGCT
CGCACGGCGGTTGAACACCGAGATTCCCGGCTATCGCTCGCTCGTCGCCAAGACTGCGCGCGCGATGCCGCTGAAGGGCG
ACGCCGATGCGGCTCTCACGCCCGCACAGACGCGCGCGCGGCTGATCGAACTCGATGCGCGCTGGGACGAGGTCGCCTAC
TGGCAGGCGATCGCCAAAAACGGCAGCGCCTATTCGCCGTTCTATCTGCTCGCCGCGCTCGATCACAAACAGGACGGCTT
CGGTAGCATCGTGCACGCCGATCCGGATCAGTCGATCTATCTGGCGATCTTCGGCCGCACGTTCGTGATCGGCATCGCCG
TGACGCTGTTCGCGCTGCTGCTCGGCTATCCGCTCGCCTACTGGATCTCGACGCTGTCGGAGCGGCGCGCGAACCTCGCG
ATGATCCTCGTGCTGATTCCGTTCTGGACCTCGGTGCTCGTGCGCGTGGCCGCGTGGATCGTGCTGCTGCAGAACGAAGG
GCTGATCAACAAGGCGCTGATCGGCAGCGGCCTGCTCGCGCATCCGCTCGCGCTGCTGTTCAACCGCGTGGGCGTCTATA
TCTCGATGACGCACATCCTGCTGCCGTTCATGATCCTGCCGCTGTACAGCGTGATGAAGTCGATTCCGCCGACCTACCAG
CGCGCGGCCGTCTCGCTCGGCAGCCATCCGTTCGCGGCGTTCTGGCGCGTGTACGTGCCGCAGACGTATCCGGGCGTCGG
CGCGGGCGCGCTGCTCGTGTTCATCCTCGCGATCGGCTACTACATCACGCCGGCGCTGCTCGGCGGCCCGAACGACCAGA
TGGTCAGCTACTACGTCGCGTACTTCACGAACGTGACGATCAACTGGGGGATGGCCTGCGCGCTCGGCGGGCTGCTGCTC
GCGGCGACGCTCGTGCTGTACGCGGTGTACGGGCGCTTCACGCGCACCAACGTGAGCCTCGGCTGA

Upstream 100 bases:

>100_bases
ACGAAGTAATCGCACCGCTGTCCCGATGCAACCGGCCGCGCGGCGCTTCGTTCGCGTCGCGCGGCATTCCGCCGGATCGC
GCTCCGGCCGGAGAGAACCG

Downstream 100 bases:

>100_bases
GCGCGGGCCGACAGGGAAGGAGAACGGAACCATGAAACTTGCCAGGCCGCTGTTTGCGCCGCACATGTCGTTCGTCGAGC
GCGCGTGGTACGTCGCGTTG

Product: spermidine/putrescine transport system permease protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MNTMTIASSPTSTAALKRELKAAEARKRTMALLLIAPLAIFLLLVFVVPIGTLLTRAVQNPEIAAALPNTVAALSHWDRK
TPPSDAAYAALATDMTKVADSDAMGALARRLNTEIPGYRSLVAKTARAMPLKGDADAALTPAQTRARLIELDARWDEVAY
WQAIAKNGSAYSPFYLLAALDHKQDGFGSIVHADPDQSIYLAIFGRTFVIGIAVTLFALLLGYPLAYWISTLSERRANLA
MILVLIPFWTSVLVRVAAWIVLLQNEGLINKALIGSGLLAHPLALLFNRVGVYISMTHILLPFMILPLYSVMKSIPPTYQ
RAAVSLGSHPFAAFWRVYVPQTYPGVGAGALLVFILAIGYYITPALLGGPNDQMVSYYVAYFTNVTINWGMACALGGLLL
AATLVLYAVYGRFTRTNVSLG

Sequences:

>Translated_421_residues
MNTMTIASSPTSTAALKRELKAAEARKRTMALLLIAPLAIFLLLVFVVPIGTLLTRAVQNPEIAAALPNTVAALSHWDRK
TPPSDAAYAALATDMTKVADSDAMGALARRLNTEIPGYRSLVAKTARAMPLKGDADAALTPAQTRARLIELDARWDEVAY
WQAIAKNGSAYSPFYLLAALDHKQDGFGSIVHADPDQSIYLAIFGRTFVIGIAVTLFALLLGYPLAYWISTLSERRANLA
MILVLIPFWTSVLVRVAAWIVLLQNEGLINKALIGSGLLAHPLALLFNRVGVYISMTHILLPFMILPLYSVMKSIPPTYQ
RAAVSLGSHPFAAFWRVYVPQTYPGVGAGALLVFILAIGYYITPALLGGPNDQMVSYYVAYFTNVTINWGMACALGGLLL
AATLVLYAVYGRFTRTNVSLG
>Mature_421_residues
MNTMTIASSPTSTAALKRELKAAEARKRTMALLLIAPLAIFLLLVFVVPIGTLLTRAVQNPEIAAALPNTVAALSHWDRK
TPPSDAAYAALATDMTKVADSDAMGALARRLNTEIPGYRSLVAKTARAMPLKGDADAALTPAQTRARLIELDARWDEVAY
WQAIAKNGSAYSPFYLLAALDHKQDGFGSIVHADPDQSIYLAIFGRTFVIGIAVTLFALLLGYPLAYWISTLSERRANLA
MILVLIPFWTSVLVRVAAWIVLLQNEGLINKALIGSGLLAHPLALLFNRVGVYISMTHILLPFMILPLYSVMKSIPPTYQ
RAAVSLGSHPFAAFWRVYVPQTYPGVGAGALLVFILAIGYYITPALLGGPNDQMVSYYVAYFTNVTINWGMACALGGLLL
AATLVLYAVYGRFTRTNVSLG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=225, Percent_Identity=33.3333333333333, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1787369, Length=223, Percent_Identity=30.4932735426009, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787713, Length=180, Percent_Identity=30.5555555555556, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 45563; Mature: 45563

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTMTIASSPTSTAALKRELKAAEARKRTMALLLIAPLAIFLLLVFVVPIGTLLTRAVQN
CCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PEIAAALPNTVAALSHWDRKTPPSDAAYAALATDMTKVADSDAMGALARRLNTEIPGYRS
CCHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHH
LVAKTARAMPLKGDADAALTPAQTRARLIELDARWDEVAYWQAIAKNGSAYSPFYLLAAL
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHEEEEC
DHKQDGFGSIVHADPDQSIYLAIFGRTFVIGIAVTLFALLLGYPLAYWISTLSERRANLA
CCCCCCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MILVLIPFWTSVLVRVAAWIVLLQNEGLINKALIGSGLLAHPLALLFNRVGVYISMTHIL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LPFMILPLYSVMKSIPPTYQRAAVSLGSHPFAAFWRVYVPQTYPGVGAGALLVFILAIGY
HHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
YITPALLGGPNDQMVSYYVAYFTNVTINWGMACALGGLLLAATLVLYAVYGRFTRTNVSL
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCC
G
C
>Mature Secondary Structure
MNTMTIASSPTSTAALKRELKAAEARKRTMALLLIAPLAIFLLLVFVVPIGTLLTRAVQN
CCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PEIAAALPNTVAALSHWDRKTPPSDAAYAALATDMTKVADSDAMGALARRLNTEIPGYRS
CCHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHH
LVAKTARAMPLKGDADAALTPAQTRARLIELDARWDEVAYWQAIAKNGSAYSPFYLLAAL
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHEEEEC
DHKQDGFGSIVHADPDQSIYLAIFGRTFVIGIAVTLFALLLGYPLAYWISTLSERRANLA
CCCCCCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MILVLIPFWTSVLVRVAAWIVLLQNEGLINKALIGSGLLAHPLALLFNRVGVYISMTHIL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LPFMILPLYSVMKSIPPTYQRAAVSLGSHPFAAFWRVYVPQTYPGVGAGALLVFILAIGY
HHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
YITPALLGGPNDQMVSYYVAYFTNVTINWGMACALGGLLLAATLVLYAVYGRFTRTNVSL
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]