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The map label for this gene is potD [H]

Identifier: 189352619

GI number: 189352619

Start: 704456

End: 705502

Strand: Direct

Name: potD [H]

Synonym: BMULJ_03850

Alternate gene names: 189352619

Gene position: 704456-705502 (Clockwise)

Preceding gene: 189352618

Following gene: 189352620

Centisome position: 28.48

GC content: 63.99

Gene sequence:

>1047_bases
ATGAACGGAACAGGTTTTGCGGTGCGCGGCACCGCGCTCGCATTGGCGCTGGCTGCCATCGGCGCATCGGCAACGGCGGC
GGAGCTGACGGTCGTCAACTTCGGCGGCGCGAACGGCGACGCGCAGAAGGTTGCCTTCAACCAGCCGTTCGAGAAGGCGA
CCGGCAACAAGGTCACCGCCGTCGAATACAACGGCGAGCAGGCCAAAGTGAAAGCGATGGTCGAAGCGAAGCACGTCAAC
TGGGACGTCGTCGAAGTCGAGTCGGGCGACCTGAATCGCGGCTGCGACGAAGGGCTCTACGAGAAGCTCGACTGGTCGAA
GATCGCGAAGAAGTCGGACCTGATTCCGGAAGCGCCGCAAGTGTGCGGCGTCGGCTTCTTCGTCTGGTCGACGGCGCTGT
CGTACAACGCCGACAAGCTGAAGACCGCGCCGACCGGCTGGGCCGATTTCTGGAACGTGAAGAAATTCCCGGGCAAGCGC
GGGATGCGCAAGGGCGCGCGCTACAACCTCGAGTTCGCGCTGATGGCCGACGGCGTCGCGCCGAAGGACGTGTACAAGGT
GCTCGGCACGAAGGCAGGCCAGGACCGTGCATTCAAGAAGCTCGACGAGCTGAAGCCGTATATCCAGTGGTGGGAAGCCG
GCGCGCAGCCGCCGCAGTTCCTCGTCGCCGGCGACGTCGTGATGTCGACCGCGTACAACGGCCGCATCGATGCCGCGCAG
AAGGAAGGCAAGAACCTGAAGGTCGTGTGGAACGGCAGCATCTACGATCTCGACTACTGGGCGATTCCGAAGGGCTCGCC
GAACAAGGCGCTTGCCGAGCAGTACATCGCCTACACGCTGACGCCGAAGCCGCAGCAGGCATACGCGCAGCACATCGCGT
ACGGGCCGGCGAACATCGCGGCGATCAAGTCGCTCGATGCGAAGACGCTTGCGAACCTGCCGAATTCGCCGGCGAACGGC
AAGAACGCGGTGCTCGAAGACATCGGGTTCTGGACCGATCACAGCGACGAGCTCGAGCAGCGCTTCGCCGCGTGGGCGAC
GAAGTAA

Upstream 100 bases:

>100_bases
CGCGTAGGCGTCCACGCCGAGCGAGCCGACGGCGCGCCGCATGTCGCCCATGACAACGTCACGCATTCACCACGACCACC
ACAAGAGGAGAGCAACGATC

Downstream 100 bases:

>100_bases
TCGCACCGCTGTCCCGATGCAACCGGCCGCGCGGCGCTTCGTTCGCGTCGCGCGGCATTCCGCCGGATCGCGCTCCGGCC
GGAGAGAACCGTTGAATACG

Product: spermidine/putrescine transport system substrate-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MNGTGFAVRGTALALALAAIGASATAAELTVVNFGGANGDAQKVAFNQPFEKATGNKVTAVEYNGEQAKVKAMVEAKHVN
WDVVEVESGDLNRGCDEGLYEKLDWSKIAKKSDLIPEAPQVCGVGFFVWSTALSYNADKLKTAPTGWADFWNVKKFPGKR
GMRKGARYNLEFALMADGVAPKDVYKVLGTKAGQDRAFKKLDELKPYIQWWEAGAQPPQFLVAGDVVMSTAYNGRIDAAQ
KEGKNLKVVWNGSIYDLDYWAIPKGSPNKALAEQYIAYTLTPKPQQAYAQHIAYGPANIAAIKSLDAKTLANLPNSPANG
KNAVLEDIGFWTDHSDELEQRFAAWATK

Sequences:

>Translated_348_residues
MNGTGFAVRGTALALALAAIGASATAAELTVVNFGGANGDAQKVAFNQPFEKATGNKVTAVEYNGEQAKVKAMVEAKHVN
WDVVEVESGDLNRGCDEGLYEKLDWSKIAKKSDLIPEAPQVCGVGFFVWSTALSYNADKLKTAPTGWADFWNVKKFPGKR
GMRKGARYNLEFALMADGVAPKDVYKVLGTKAGQDRAFKKLDELKPYIQWWEAGAQPPQFLVAGDVVMSTAYNGRIDAAQ
KEGKNLKVVWNGSIYDLDYWAIPKGSPNKALAEQYIAYTLTPKPQQAYAQHIAYGPANIAAIKSLDAKTLANLPNSPANG
KNAVLEDIGFWTDHSDELEQRFAAWATK
>Mature_348_residues
MNGTGFAVRGTALALALAAIGASATAAELTVVNFGGANGDAQKVAFNQPFEKATGNKVTAVEYNGEQAKVKAMVEAKHVN
WDVVEVESGDLNRGCDEGLYEKLDWSKIAKKSDLIPEAPQVCGVGFFVWSTALSYNADKLKTAPTGWADFWNVKKFPGKR
GMRKGARYNLEFALMADGVAPKDVYKVLGTKAGQDRAFKKLDELKPYIQWWEAGAQPPQFLVAGDVVMSTAYNGRIDAAQ
KEGKNLKVVWNGSIYDLDYWAIPKGSPNKALAEQYIAYTLTPKPQQAYAQHIAYGPANIAAIKSLDAKTLANLPNSPANG
KNAVLEDIGFWTDHSDELEQRFAAWATK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=271, Percent_Identity=26.5682656826568, Blast_Score=71, Evalue=8e-14,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37788; Mature: 37788

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGTGFAVRGTALALALAAIGASATAAELTVVNFGGANGDAQKVAFNQPFEKATGNKVTA
CCCCCEEEHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCEEECCCCHHHCCCCEEEE
VEYNGEQAKVKAMVEAKHVNWDVVEVESGDLNRGCDEGLYEKLDWSKIAKKSDLIPEAPQ
EEECCCHHEEEEEEEHCCCCEEEEEECCCCCCCCCHHHHHHHCCHHHHHHHCCCCCCCHH
VCGVGFFVWSTALSYNADKLKTAPTGWADFWNVKKFPGKRGMRKGARYNLEFALMADGVA
HHHHHHHHHHHHHHCCHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCC
PKDVYKVLGTKAGQDRAFKKLDELKPYIQWWEAGAQPPQFLVAGDVVMSTAYNGRIDAAQ
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHCCCCCCCCHH
KEGKNLKVVWNGSIYDLDYWAIPKGSPNKALAEQYIAYTLTPKPQQAYAQHIAYGPANIA
HCCCEEEEEECCCEEEEEEEECCCCCCCHHHHHHHEEEEECCCHHHHHHHHHHCCCCHHH
AIKSLDAKTLANLPNSPANGKNAVLEDIGFWTDHSDELEQRFAAWATK
HHHHCCHHHHHCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MNGTGFAVRGTALALALAAIGASATAAELTVVNFGGANGDAQKVAFNQPFEKATGNKVTA
CCCCCEEEHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCEEECCCCHHHCCCCEEEE
VEYNGEQAKVKAMVEAKHVNWDVVEVESGDLNRGCDEGLYEKLDWSKIAKKSDLIPEAPQ
EEECCCHHEEEEEEEHCCCCEEEEEECCCCCCCCCHHHHHHHCCHHHHHHHCCCCCCCHH
VCGVGFFVWSTALSYNADKLKTAPTGWADFWNVKKFPGKRGMRKGARYNLEFALMADGVA
HHHHHHHHHHHHHHCCHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCC
PKDVYKVLGTKAGQDRAFKKLDELKPYIQWWEAGAQPPQFLVAGDVVMSTAYNGRIDAAQ
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHCCCCCCCCHH
KEGKNLKVVWNGSIYDLDYWAIPKGSPNKALAEQYIAYTLTPKPQQAYAQHIAYGPANIA
HCCCEEEEEECCCEEEEEEEECCCCCCCHHHHHHHEEEEECCCHHHHHHHHHHCCCCHHH
AIKSLDAKTLANLPNSPANGKNAVLEDIGFWTDHSDELEQRFAAWATK
HHHHCCHHHHHCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]