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The map label for this gene is oprM [H]

Identifier: 189352599

GI number: 189352599

Start: 679804

End: 681288

Strand: Reverse

Name: oprM [H]

Synonym: BMULJ_03830

Alternate gene names: 189352599

Gene position: 681288-679804 (Counterclockwise)

Preceding gene: 189352600

Following gene: 189352598

Centisome position: 27.55

GC content: 71.18

Gene sequence:

>1485_bases
ATGAAGCCTCTCTTCCCGCCCCGCCGCCCCGCGCTCGCGCTCTGCACGGCCGCGTGCCTGCTCGCCGGCTGCACGGTCGG
CCCCGACTACCGCGGTGCACCCGCCGCGTCCGACACGCCGACCTTCGTCCGCGCGCCGGCCACCGGCATCGACGCGGGCG
CACCCGCGCCGAGCGCATGGTGGCATGCGCTGCACGATCGCCGGCTCGACGCGCTGATCGCCGCCGCGCTCGCGCACAAT
CCGGATCTGCATGCCGCACAGGCGCGATTGCGCGCCGCGCGCGCGCAGCTCGCGCAGCAGCGCGCGGCCGAACTGCCGAA
GGCATCCGCCACCGTCGCGGCGATCCGCATGCGCGAGCCCGACGTCAGTACACTCGGCGCGCTGTTGCCGTCGAACGGTT
CGGGCCAGTCGGGCAGCGCGCCGGCGACGCTCGGCACGGGCCCGCTGCAGCTCTATTCGGCCGGCTTCGACGCGACCTGG
GAAATCGATCTGTTCGGCGGCACGCGGCGCGCGATCGAGGCGGCGTCCGCGCAGGCCGACGCGGTCGACGCGGATCTGGC
CGACACGCAGGTGTCGATCGCGGCCGAAGTCGCGACCGCCTACGTCGACCTGCGCGACCGGCAGCAGCGTCTCGCGCTGT
CGCGCCGCACGGCCGAACTGCAGCAGCAGATGCTCGCGCTCACGCAGCAACGCCGCGCGCGCGGCGTTGCGGCCGACGCA
GACATCGAGCGGCTCACCACGCAGGTCGAAAACACGCGCGCGTCGTTGATTCCGCTCGATGCGCAGGTCACCGAATCGCT
CGACCGGCTCGCGATCCTGACCGGCCGCGAGCCGGGCGCGCTCGATGCCGAACTGTCGACCGACGAGGCATCGTTGCCGA
CGCTGCCCGCGTCGGCGCCGGTCGGCGATCCGGCCGCGCTGCTCAAGCGCCGCCCGGACATTCGCGCGGCCGAGCGGCGG
CTTGCGTCGAGCAATGCGCAGATCGGCGAGCATATCGCCGACTATTTTCCGAAGGTGACGCTGCTCGGCGATATCGGCTT
CAGCGCGACCGATCCCGCCCATCTGTTCCGCAAGCAGAATGCGTCGTGGATCGGCGCGCCGTATCTGCAGTGGAACATTC
TCGATTTCGGTCGCACGCGCGGCGCCGTGCGGGCGGCCGAAGCGTCGCGCGACGAAGCCGATGCGAACTACCGGAAAGCC
GTGCTCGGCGCGCTGCAGGACGCGAATACCGCGCTGCAGCGCTACGGGCACCAGCGCGACCATGTCGTTGCGCTGACCAA
GGTGCAGGCATCGGCCACGCATTCGGCGACGCTGATGAGCGAGCGCTATCGTGCGGGCGTCGCATCGATGATCGATCTGC
TCGATACGCAGCGCGAATCGCTCGCGGCCCGGCAGAACGTGATCGCGGCGCAGGCTGAGCTGATCAAGGACTACGTGTCG
GTGCAGAAAAGTCTCGGGCTCGGCTGGCAGGCGGGCGACGCGTAA

Upstream 100 bases:

>100_bases
TACCTGCTGGGACTCGCGCTGCTCGCCTGCGTGCCGCTCGCGCTGCTGCTGAAGACGCCGCGCACGCCGCAGCCGATGTC
GTCCGGCCACTGAACCCGCG

Downstream 100 bases:

>100_bases
CGCGTCGGTCGCATCGGCGTCGCCGGCCGGTCGCGGCAGGCGCGGAGACAGGACGGCGACACGGATTCGGCACGGCGGGA
TTCGGCTTCGGCACGGCGCC

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MKPLFPPRRPALALCTAACLLAGCTVGPDYRGAPAASDTPTFVRAPATGIDAGAPAPSAWWHALHDRRLDALIAAALAHN
PDLHAAQARLRAARAQLAQQRAAELPKASATVAAIRMREPDVSTLGALLPSNGSGQSGSAPATLGTGPLQLYSAGFDATW
EIDLFGGTRRAIEAASAQADAVDADLADTQVSIAAEVATAYVDLRDRQQRLALSRRTAELQQQMLALTQQRRARGVAADA
DIERLTTQVENTRASLIPLDAQVTESLDRLAILTGREPGALDAELSTDEASLPTLPASAPVGDPAALLKRRPDIRAAERR
LASSNAQIGEHIADYFPKVTLLGDIGFSATDPAHLFRKQNASWIGAPYLQWNILDFGRTRGAVRAAEASRDEADANYRKA
VLGALQDANTALQRYGHQRDHVVALTKVQASATHSATLMSERYRAGVASMIDLLDTQRESLAARQNVIAAQAELIKDYVS
VQKSLGLGWQAGDA

Sequences:

>Translated_494_residues
MKPLFPPRRPALALCTAACLLAGCTVGPDYRGAPAASDTPTFVRAPATGIDAGAPAPSAWWHALHDRRLDALIAAALAHN
PDLHAAQARLRAARAQLAQQRAAELPKASATVAAIRMREPDVSTLGALLPSNGSGQSGSAPATLGTGPLQLYSAGFDATW
EIDLFGGTRRAIEAASAQADAVDADLADTQVSIAAEVATAYVDLRDRQQRLALSRRTAELQQQMLALTQQRRARGVAADA
DIERLTTQVENTRASLIPLDAQVTESLDRLAILTGREPGALDAELSTDEASLPTLPASAPVGDPAALLKRRPDIRAAERR
LASSNAQIGEHIADYFPKVTLLGDIGFSATDPAHLFRKQNASWIGAPYLQWNILDFGRTRGAVRAAEASRDEADANYRKA
VLGALQDANTALQRYGHQRDHVVALTKVQASATHSATLMSERYRAGVASMIDLLDTQRESLAARQNVIAAQAELIKDYVS
VQKSLGLGWQAGDA
>Mature_494_residues
MKPLFPPRRPALALCTAACLLAGCTVGPDYRGAPAASDTPTFVRAPATGIDAGAPAPSAWWHALHDRRLDALIAAALAHN
PDLHAAQARLRAARAQLAQQRAAELPKASATVAAIRMREPDVSTLGALLPSNGSGQSGSAPATLGTGPLQLYSAGFDATW
EIDLFGGTRRAIEAASAQADAVDADLADTQVSIAAEVATAYVDLRDRQQRLALSRRTAELQQQMLALTQQRRARGVAADA
DIERLTTQVENTRASLIPLDAQVTESLDRLAILTGREPGALDAELSTDEASLPTLPASAPVGDPAALLKRRPDIRAAERR
LASSNAQIGEHIADYFPKVTLLGDIGFSATDPAHLFRKQNASWIGAPYLQWNILDFGRTRGAVRAAEASRDEADANYRKA
VLGALQDANTALQRYGHQRDHVVALTKVQASATHSATLMSERYRAGVASMIDLLDTQRESLAARQNVIAAQAELIKDYVS
VQKSLGLGWQAGDA

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=480, Percent_Identity=24.5833333333333, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI2367348, Length=498, Percent_Identity=27.1084337349398, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52462; Mature: 52462

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLFPPRRPALALCTAACLLAGCTVGPDYRGAPAASDTPTFVRAPATGIDAGAPAPSAW
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHH
WHALHDRRLDALIAAALAHNPDLHAAQARLRAARAQLAQQRAAELPKASATVAAIRMREP
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCC
DVSTLGALLPSNGSGQSGSAPATLGTGPLQLYSAGFDATWEIDLFGGTRRAIEAASAQAD
CHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHEECCCCCEEEEEECCCCHHHHHHHHCHHH
AVDADLADTQVSIAAEVATAYVDLRDRQQRLALSRRTAELQQQMLALTQQRRARGVAADA
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DIERLTTQVENTRASLIPLDAQVTESLDRLAILTGREPGALDAELSTDEASLPTLPASAP
HHHHHHHHHHHCCHHEEECCHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
VGDPAALLKRRPDIRAAERRLASSNAQIGEHIADYFPKVTLLGDIGFSATDPAHLFRKQN
CCCHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHCC
ASWIGAPYLQWNILDFGRTRGAVRAAEASRDEADANYRKAVLGALQDANTALQRYGHQRD
CCEEECCCEEEEEHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
HVVALTKVQASATHSATLMSERYRAGVASMIDLLDTQRESLAARQNVIAAQAELIKDYVS
CEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQKSLGLGWQAGDA
HHHHHCCCCCCCCC
>Mature Secondary Structure
MKPLFPPRRPALALCTAACLLAGCTVGPDYRGAPAASDTPTFVRAPATGIDAGAPAPSAW
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHH
WHALHDRRLDALIAAALAHNPDLHAAQARLRAARAQLAQQRAAELPKASATVAAIRMREP
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCC
DVSTLGALLPSNGSGQSGSAPATLGTGPLQLYSAGFDATWEIDLFGGTRRAIEAASAQAD
CHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHEECCCCCEEEEEECCCCHHHHHHHHCHHH
AVDADLADTQVSIAAEVATAYVDLRDRQQRLALSRRTAELQQQMLALTQQRRARGVAADA
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DIERLTTQVENTRASLIPLDAQVTESLDRLAILTGREPGALDAELSTDEASLPTLPASAP
HHHHHHHHHHHCCHHEEECCHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
VGDPAALLKRRPDIRAAERRLASSNAQIGEHIADYFPKVTLLGDIGFSATDPAHLFRKQN
CCCHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHCC
ASWIGAPYLQWNILDFGRTRGAVRAAEASRDEADANYRKAVLGALQDANTALQRYGHQRD
CCEEECCCEEEEEHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
HVVALTKVQASATHSATLMSERYRAGVASMIDLLDTQRESLAARQNVIAAQAELIKDYVS
CEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQKSLGLGWQAGDA
HHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]