Click here to switch to the map view.

The map label for this gene is phaZ1 [H]

Identifier: 189352413

GI number: 189352413

Start: 470873

End: 471982

Strand: Direct

Name: phaZ1 [H]

Synonym: BMULJ_03632

Alternate gene names: 189352413

Gene position: 470873-471982 (Clockwise)

Preceding gene: 189352408

Following gene: 189352414

Centisome position: 19.04

GC content: 71.44

Gene sequence:

>1110_bases
ATGACCAAAAGTCTGACGAAAATGTGGCTGGGGGGCATGAAACGCGTGCTATCTCCGGCCGCGAAGCGTGCCGCGCGCGA
TGCCCGTCGGCTCACGCGCGACGTGCTCGACGCCGCCGCGTCCCCGGCTGCATCCGAAGTATCCATGCCGCGCGAGTCGC
GCGTGCGTCCGCGCGCGGCCGCGTGGGCCGCCGGCGAGTGGACACGCGGCGAACATCCGATGGCGCCCGCGCTCGGGCGG
CTGGTCCAGCAGCTCGCCTACGCGCTCTACGTGCCGCCCGGGCACCGGCGCGAAGCGATGCCGCTCGTCGTGATGCTGCA
CGGCTGCCAGCAGACCGCCGACGCGTTCGCGCAGGGCACGCGGATGAACCTGCTCGCCGACAAGCACGGCTTCGCGGTGC
TGTACCCCGAGCAGTCGATGCGCGCGCATTCGCACGGCTGCTGGCACTGGTACGAAGATACCGACCGCGCGGGCCGCGGC
GAAGCCGATGCGGTCGCTTCGCTCGTCGACGCGCTCGTCGACGAGCACGGCTTCGATGCGTCACGCGTCTACGTCGCGGG
ACTGTCGGCCGGCGCCGGGCTCGCGTCGCTGCTGGCGCTGCACCACCCCGACCGGTTCGCCGCGGTCGCGCTGCATTCGG
GCCCCGCGCTCGGCGAAGCGAATTCCGGCATTTCGGCGATGGACGTGATGCGGCGCGGGCTGCGCGAGAACCCGGCGGCC
GTCGTCGACGCGCTCGTCGATGCCGACAGCTATCCCGGCATGCCGGCGCTGATCGTGCAGGGCGACGGCGATCGCGTCGT
CGCGCCGAAGAACGCCGATGAACTGGCCGTCCAGTTCATGCGCCTGAACGGGCTCGCAGACAGCCGCGGCGCGCTGCGCG
GCGGCGAGCGCGTGGAGACGCGGCAAGCCGGTGCGCGGATCGTCGACGTGCGGCGCGACGGCGAATCGGTCGTGCGGCTT
TGCCACGTGAAGGGGCTCGATCACGCATGGTCGGGCGGCGACGAAGCGGTGCCGTTCCATGCCGCCGTCGGTCCCGATGC
GAGCGCGATGGTCTGGTCCTTTTTCGAAACGCATCGGCGCACTGTGGCGACCGAACGACGCACCGACTGA

Upstream 100 bases:

>100_bases
GAGCAGGAAAGATCCCACGAAGAGTGCTATTGTGCATTGCACAAATACAGACGATACGCCTTGCGAGGCGTCCCCATTTT
CCTGACCGCGAGTCCGATCG

Downstream 100 bases:

>100_bases
CCGGCACTGGCATTCGACTAGGGTTTTCCCTATAATCAGGGAAAACCCTAGTCAACGAATGCGAGATCGACCATGTACCT
GCTGAGCCACCTGTTCCTGA

Product: poly(3-hydroxybutyrate) depolymerase

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MTKSLTKMWLGGMKRVLSPAAKRAARDARRLTRDVLDAAASPAASEVSMPRESRVRPRAAAWAAGEWTRGEHPMAPALGR
LVQQLAYALYVPPGHRREAMPLVVMLHGCQQTADAFAQGTRMNLLADKHGFAVLYPEQSMRAHSHGCWHWYEDTDRAGRG
EADAVASLVDALVDEHGFDASRVYVAGLSAGAGLASLLALHHPDRFAAVALHSGPALGEANSGISAMDVMRRGLRENPAA
VVDALVDADSYPGMPALIVQGDGDRVVAPKNADELAVQFMRLNGLADSRGALRGGERVETRQAGARIVDVRRDGESVVRL
CHVKGLDHAWSGGDEAVPFHAAVGPDASAMVWSFFETHRRTVATERRTD

Sequences:

>Translated_369_residues
MTKSLTKMWLGGMKRVLSPAAKRAARDARRLTRDVLDAAASPAASEVSMPRESRVRPRAAAWAAGEWTRGEHPMAPALGR
LVQQLAYALYVPPGHRREAMPLVVMLHGCQQTADAFAQGTRMNLLADKHGFAVLYPEQSMRAHSHGCWHWYEDTDRAGRG
EADAVASLVDALVDEHGFDASRVYVAGLSAGAGLASLLALHHPDRFAAVALHSGPALGEANSGISAMDVMRRGLRENPAA
VVDALVDADSYPGMPALIVQGDGDRVVAPKNADELAVQFMRLNGLADSRGALRGGERVETRQAGARIVDVRRDGESVVRL
CHVKGLDHAWSGGDEAVPFHAAVGPDASAMVWSFFETHRRTVATERRTD
>Mature_368_residues
TKSLTKMWLGGMKRVLSPAAKRAARDARRLTRDVLDAAASPAASEVSMPRESRVRPRAAAWAAGEWTRGEHPMAPALGRL
VQQLAYALYVPPGHRREAMPLVVMLHGCQQTADAFAQGTRMNLLADKHGFAVLYPEQSMRAHSHGCWHWYEDTDRAGRGE
ADAVASLVDALVDEHGFDASRVYVAGLSAGAGLASLLALHHPDRFAAVALHSGPALGEANSGISAMDVMRRGLRENPAAV
VDALVDADSYPGMPALIVQGDGDRVVAPKNADELAVQFMRLNGLADSRGALRGGERVETRQAGARIVDVRRDGESVVRLC
HVKGLDHAWSGGDEAVPFHAAVGPDASAMVWSFFETHRRTVATERRTD

Specific function: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P- nitrophenylbutyrate as substrates [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 39709; Mature: 39578

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSLTKMWLGGMKRVLSPAAKRAARDARRLTRDVLDAAASPAASEVSMPRESRVRPRAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHCCCCHHH
AWAAGEWTRGEHPMAPALGRLVQQLAYALYVPPGHRREAMPLVVMLHGCQQTADAFAQGT
HCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
RMNLLADKHGFAVLYPEQSMRAHSHGCWHWYEDTDRAGRGEADAVASLVDALVDEHGFDA
CEEEEECCCCCEEECCCHHHHHCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
SRVYVAGLSAGAGLASLLALHHPDRFAAVALHSGPALGEANSGISAMDVMRRGLRENPAA
CEEEEEECCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHH
VVDALVDADSYPGMPALIVQGDGDRVVAPKNADELAVQFMRLNGLADSRGALRGGERVET
HHHHHHCCCCCCCCCEEEEECCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
RQAGARIVDVRRDGESVVRLCHVKGLDHAWSGGDEAVPFHAAVGPDASAMVWSFFETHRR
HHCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHH
TVATERRTD
HHHHHCCCC
>Mature Secondary Structure 
TKSLTKMWLGGMKRVLSPAAKRAARDARRLTRDVLDAAASPAASEVSMPRESRVRPRAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHCCCCHHH
AWAAGEWTRGEHPMAPALGRLVQQLAYALYVPPGHRREAMPLVVMLHGCQQTADAFAQGT
HCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
RMNLLADKHGFAVLYPEQSMRAHSHGCWHWYEDTDRAGRGEADAVASLVDALVDEHGFDA
CEEEEECCCCCEEECCCHHHHHCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
SRVYVAGLSAGAGLASLLALHHPDRFAAVALHSGPALGEANSGISAMDVMRRGLRENPAA
CEEEEEECCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHH
VVDALVDADSYPGMPALIVQGDGDRVVAPKNADELAVQFMRLNGLADSRGALRGGERVET
HHHHHHCCCCCCCCCEEEEECCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
RQAGARIVDVRRDGESVVRLCHVKGLDHAWSGGDEAVPFHAAVGPDASAMVWSFFETHRR
HHCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHH
TVATERRTD
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8269961 [H]