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The map label for this gene is metY [H]

Identifier: 189352264

GI number: 189352264

Start: 309921

End: 311216

Strand: Reverse

Name: metY [H]

Synonym: BMULJ_03483

Alternate gene names: 189352264

Gene position: 311216-309921 (Counterclockwise)

Preceding gene: 189352266

Following gene: 189352258

Centisome position: 12.58

GC content: 66.28

Gene sequence:

>1296_bases
ATGACCGATCAGGCCTCTTCGAAGTGGCGGCTCGAAACGCTCGCCGTCCATGGCGGCTACCGCCCCGATCCGACGACGCG
CGCCGTCGCGGTGCCGATCTACCAGACCGTCGCGTATGCGTTCGACGACACGCAGCACGGCGCCGACCTATTCGACCTGA
AGGTCCAGGGCAACATCTATACGCGCATCATGAACCCGACGACCGACGTGCTCGAACAGCGGCTCGCCGCGCTCGAGGGC
GGCGTCGGCGCGCTCGCGCTGGCGTCGGGTCAGGCAGCCGTCACGTATGCGATCCAGACGATCGCGGAAGCGGGCGACAA
CATCGTCGCGGCCAGTTCGCTCTACGGCGGCACCTACAACCTGTTCGCGCATACGCTGCCGCAATACGGCATTACGACGC
GCTTCGCCGATCCGCGCGACCCCGCATCGTTCGCACCGCTGATCGATGCGCACACGAAGGCGATCTTCGCGGAATCGGTC
GGCAATCCGCTCGGCAACATCACCGATATCGCGGCGCTCGCCGACATCGCGCATCGCCACGGCATTCCGCTCATCGTCGA
CAACACGGTGCCGTCGCCGTATCTGCTGCGCCCGTTCGAGCATGGCGCCGACATCGTCGTGCATTCGCTGACGAAGTATC
TCGGCGGTCACGGCACGAGCCTCGGCGGCGCGATCGTCGATTCGGGCAGGTTCCCGTGGCCCGACCATGCGGACCGCTTC
AAGCGGCTCAACGAACCGGACGTCAGCTATCACGGCGTCGTCTATACGGAAGCGTTCGGGCCGGCCGCCTATATCGGCCG
CGCGCGCGTCGTGCCGCTGCGCAACATGGGCGCCGCGATCTCGCCGTTCAACGCGTTCCAGATTCTGCAAGGGATCGAGA
CGCTCGCGCTGCGCATCGAGCGCATCAGCGACAACGCGCTGAAGATCGCGCAGCATCTCGCGCGTCACGAACGCGTCGAG
TGGGTCAACTATGCGGGGCTGCCCGACCATCCCGATCATCCGCTCGTCGCGCGCTATCTGTCGGGACGTGCGCCGGGCCT
GCTGACGTTCGGCGTGAAAGGCGGCCGCGCAGGCGGTGCGAAGTTCCAGGATGCGCTGAAGCTGTTCACGCGGCTCGTCA
ACATCGGCGACACGAAATCGCTCGCGACGCATCCGGCGTCGACCACGCATCGGCAGCTGTCGCCGGCCGAACTCGCGAAG
GCCGGCGTGAAGGAGGAAACGGTGCGGCTGTCGATCGGCATCGAGCATATCGACGATCTGATCGCCGATCTCGATCAGGC
GCTCGCGCAGGTCTGA

Upstream 100 bases:

>100_bases
ATGCGCGCTGCGCCGATAAGCAACGCACCATTCGTTCTAAGCTTCGCGCGCGCTGCGCTCCTATAATCGCCCGATCATCA
CAACAACAGACGGAGCACGC

Downstream 100 bases:

>100_bases
TCGGGCCGCCGCGCGCGAGACGCGCCGCGATCGGCATGCATCACGCGCGGCCGCGTTTGCCGCGCCGGCGCTGCTCGAGC
CAGTCGAGCAGTTGCCGGCC

Product: O-acetylhomoserine (thiol)-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MTDQASSKWRLETLAVHGGYRPDPTTRAVAVPIYQTVAYAFDDTQHGADLFDLKVQGNIYTRIMNPTTDVLEQRLAALEG
GVGALALASGQAAVTYAIQTIAEAGDNIVAASSLYGGTYNLFAHTLPQYGITTRFADPRDPASFAPLIDAHTKAIFAESV
GNPLGNITDIAALADIAHRHGIPLIVDNTVPSPYLLRPFEHGADIVVHSLTKYLGGHGTSLGGAIVDSGRFPWPDHADRF
KRLNEPDVSYHGVVYTEAFGPAAYIGRARVVPLRNMGAAISPFNAFQILQGIETLALRIERISDNALKIAQHLARHERVE
WVNYAGLPDHPDHPLVARYLSGRAPGLLTFGVKGGRAGGAKFQDALKLFTRLVNIGDTKSLATHPASTTHRQLSPAELAK
AGVKEETVRLSIGIEHIDDLIADLDQALAQV

Sequences:

>Translated_431_residues
MTDQASSKWRLETLAVHGGYRPDPTTRAVAVPIYQTVAYAFDDTQHGADLFDLKVQGNIYTRIMNPTTDVLEQRLAALEG
GVGALALASGQAAVTYAIQTIAEAGDNIVAASSLYGGTYNLFAHTLPQYGITTRFADPRDPASFAPLIDAHTKAIFAESV
GNPLGNITDIAALADIAHRHGIPLIVDNTVPSPYLLRPFEHGADIVVHSLTKYLGGHGTSLGGAIVDSGRFPWPDHADRF
KRLNEPDVSYHGVVYTEAFGPAAYIGRARVVPLRNMGAAISPFNAFQILQGIETLALRIERISDNALKIAQHLARHERVE
WVNYAGLPDHPDHPLVARYLSGRAPGLLTFGVKGGRAGGAKFQDALKLFTRLVNIGDTKSLATHPASTTHRQLSPAELAK
AGVKEETVRLSIGIEHIDDLIADLDQALAQV
>Mature_430_residues
TDQASSKWRLETLAVHGGYRPDPTTRAVAVPIYQTVAYAFDDTQHGADLFDLKVQGNIYTRIMNPTTDVLEQRLAALEGG
VGALALASGQAAVTYAIQTIAEAGDNIVAASSLYGGTYNLFAHTLPQYGITTRFADPRDPASFAPLIDAHTKAIFAESVG
NPLGNITDIAALADIAHRHGIPLIVDNTVPSPYLLRPFEHGADIVVHSLTKYLGGHGTSLGGAIVDSGRFPWPDHADRFK
RLNEPDVSYHGVVYTEAFGPAAYIGRARVVPLRNMGAAISPFNAFQILQGIETLALRIERISDNALKIAQHLARHERVEW
VNYAGLPDHPDHPLVARYLSGRAPGLLTFGVKGGRAGGAKFQDALKLFTRLVNIGDTKSLATHPASTTHRQLSPAELAKA
GVKEETVRLSIGIEHIDDLIADLDQALAQV

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=404, Percent_Identity=33.9108910891089, Blast_Score=192, Evalue=5e-49,
Organism=Homo sapiens, GI299473759, Length=419, Percent_Identity=31.2649164677804, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI25453487, Length=418, Percent_Identity=31.1004784688995, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1790375, Length=416, Percent_Identity=30.2884615384615, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1789383, Length=429, Percent_Identity=27.039627039627, Blast_Score=135, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI71980708, Length=421, Percent_Identity=30.6413301662708, Blast_Score=204, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI17560056, Length=421, Percent_Identity=34.916864608076, Blast_Score=192, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17538055, Length=420, Percent_Identity=33.8095238095238, Blast_Score=189, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6323333, Length=434, Percent_Identity=46.5437788018433, Blast_Score=367, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6319307, Length=438, Percent_Identity=32.648401826484, Blast_Score=187, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6321170, Length=354, Percent_Identity=29.0960451977401, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6321254, Length=444, Percent_Identity=25.2252252252252, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321904, Length=436, Percent_Identity=25, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI19922554, Length=419, Percent_Identity=33.890214797136, Blast_Score=206, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46346; Mature: 46215

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDQASSKWRLETLAVHGGYRPDPTTRAVAVPIYQTVAYAFDDTQHGADLFDLKVQGNIY
CCCCCCCCEEEEEEEEECCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCEEEEEECCCEE
TRIMNPTTDVLEQRLAALEGGVGALALASGQAAVTYAIQTIAEAGDNIVAASSLYGGTYN
EEEECCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEHHHCCCHHH
LFAHTLPQYGITTRFADPRDPASFAPLIDAHTKAIFAESVGNPLGNITDIAALADIAHRH
HHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
GIPLIVDNTVPSPYLLRPFEHGADIVVHSLTKYLGGHGTSLGGAIVDSGRFPWPDHADRF
CCCEEECCCCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHH
KRLNEPDVSYHGVVYTEAFGPAAYIGRARVVPLRNMGAAISPFNAFQILQGIETLALRIE
HHCCCCCCCCCEEEEEECCCCHHHHCCEEEEEHHCCCCCCCCHHHHHHHHHHHHHHHHHH
RISDNALKIAQHLARHERVEWVNYAGLPDHPDHPLVARYLSGRAPGLLTFGVKGGRAGGA
HHCCHHHHHHHHHHHHHHHCEEEECCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCCCCH
KFQDALKLFTRLVNIGDTKSLATHPASTTHRQLSPAELAKAGVKEETVRLSIGIEHIDDL
HHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCHHHHHHCCCCCCEEEEEECHHHHHHH
IADLDQALAQV
HHHHHHHHHCC
>Mature Secondary Structure 
TDQASSKWRLETLAVHGGYRPDPTTRAVAVPIYQTVAYAFDDTQHGADLFDLKVQGNIY
CCCCCCCEEEEEEEEECCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCEEEEEECCCEE
TRIMNPTTDVLEQRLAALEGGVGALALASGQAAVTYAIQTIAEAGDNIVAASSLYGGTYN
EEEECCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEHHHCCCHHH
LFAHTLPQYGITTRFADPRDPASFAPLIDAHTKAIFAESVGNPLGNITDIAALADIAHRH
HHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
GIPLIVDNTVPSPYLLRPFEHGADIVVHSLTKYLGGHGTSLGGAIVDSGRFPWPDHADRF
CCCEEECCCCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHH
KRLNEPDVSYHGVVYTEAFGPAAYIGRARVVPLRNMGAAISPFNAFQILQGIETLALRIE
HHCCCCCCCCCEEEEEECCCCHHHHCCEEEEEHHCCCCCCCCHHHHHHHHHHHHHHHHHH
RISDNALKIAQHLARHERVEWVNYAGLPDHPDHPLVARYLSGRAPGLLTFGVKGGRAGGA
HHCCHHHHHHHHHHHHHHHCEEEECCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCCCCH
KFQDALKLFTRLVNIGDTKSLATHPASTTHRQLSPAELAKAGVKEETVRLSIGIEHIDDL
HHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCHHHHHHCCCCCCEEEEEECHHHHHHH
IADLDQALAQV
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]