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The map label for this gene is thiO [H]

Identifier: 189352235

GI number: 189352235

Start: 276201

End: 277352

Strand: Reverse

Name: thiO [H]

Synonym: BMULJ_03453

Alternate gene names: 189352235

Gene position: 277352-276201 (Counterclockwise)

Preceding gene: 189352236

Following gene: 189352234

Centisome position: 11.21

GC content: 72.92

Gene sequence:

>1152_bases
ATGAGTGAGACCGGTCATTACGACGTCGCTGTCGTCGGCGGCGGCCTGGTCGGCGCGTCGGCCGCGCTCGCGCTCGCGCG
GCGCGGGCTGCGCGTCGCGCTGTTCGAGCGCCGCGACTGCGGCGCGCAGGCGAGCGGCGTGAACTACGGCGGCGTGCGCT
GCCAGGGCCGTCCCGCCGAACAGCTGCCGCTCGCGCTGCGCGCACGGCGCATCTGGGATCGTCTGCCGGAACGGATCGGC
ATCGACGGCGAATTCGTCGTGTCGGGCCACCTGCGGCTCGCGCGCAGCGACGCCGATCTCGAGGCGCTCCACGCCTACGC
GGCGCTCGCAGCCGAACACGGGCTGCCGCTGCGCGTGATGCACGGCGACGCATTCCGCCGCCGCTATCCGTGGCTTGGCC
GCGCCGCGCTCGGCGGCTCGCTCTGCGAGACCGACGGCCACGCGAACCCGCGCATCGTGTCGCCGGCCTTCGCGCGTGCG
GCGCGCGCAGCCGGCGCGGACGTATTCGAGCACACGCCGGTCGACGACGTCCGGCACGACGGCACGCGCTTTCGGATCCA
TGCGGCCGGCCGCGCCTGCACGTCGACCTGGCTCGTCAATGCGGCCGGCGCCTGGGCGAACGCGATCGCCGAACGCTTCG
GCGAAGCGGTGCCGATGGAGCCCATCTACCCGAACATGTGGGTGACCGAGCCGCTGCCGCCGTTCATCGAGCACAACCTC
GGCGTCTACGGCGGCGGCGTCTATGCGCGCCAGGTCGCGCGCGGCAACTGCGTGATCGGCGGCGGACGCGGACGCGGCGA
CGGCGAGTTCGGCCAGCCGTCCGTCGACACGACGCGCGCCGTGATGCGCGACGCCTGCGCGCTGCTGCCCGCGCTGCGCG
ACGCGCTGCTGATCCGCACGTGGAGCGGCGTCGAAGGCTGCACGCCCGACCACAACCCGATCGTCGGCGCGAGCCGCACG
ACGCCGAAGCTGCTGCACGCGTTCGGTTTTTCGGGCGGCGGGTTTCTGCTCGCGCCCGGTGTCGGCGACGTGCTCGCCGA
TCTCGTGACGACCGGCGAAACCGTGACGCCGCTCGATGCGTTTTCGATCGGCCGCTTCGCACCGCAAGCCGAACCGACCA
TCCCTTGCCGTCAACAGGAGACCCAACGATGA

Upstream 100 bases:

>100_bases
GACGTCGGCCGGTTGCGTGCGCAACCGCCGGTGAAGCCGTTTCCGATCGCGGCCGCCCTCGCCGGCGACGACGTCGTCGC
GATTCCCGACGAGGCGCGCG

Downstream 100 bases:

>100_bases
AACTGACCGGAACGATCGCGGCGGCCGCCGCGCTCTGCATCGCAAGCGCGACCGCGTCCGCGCCTGCGTTCGCGCAAACG
AAGACGATCTACGTCGGCAT

Product: glycine/D-amino acid oxidase

Products: NA

Alternate protein names: GO [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MSETGHYDVAVVGGGLVGASAALALARRGLRVALFERRDCGAQASGVNYGGVRCQGRPAEQLPLALRARRIWDRLPERIG
IDGEFVVSGHLRLARSDADLEALHAYAALAAEHGLPLRVMHGDAFRRRYPWLGRAALGGSLCETDGHANPRIVSPAFARA
ARAAGADVFEHTPVDDVRHDGTRFRIHAAGRACTSTWLVNAAGAWANAIAERFGEAVPMEPIYPNMWVTEPLPPFIEHNL
GVYGGGVYARQVARGNCVIGGGRGRGDGEFGQPSVDTTRAVMRDACALLPALRDALLIRTWSGVEGCTPDHNPIVGASRT
TPKLLHAFGFSGGGFLLAPGVGDVLADLVTTGETVTPLDAFSIGRFAPQAEPTIPCRQQETQR

Sequences:

>Translated_383_residues
MSETGHYDVAVVGGGLVGASAALALARRGLRVALFERRDCGAQASGVNYGGVRCQGRPAEQLPLALRARRIWDRLPERIG
IDGEFVVSGHLRLARSDADLEALHAYAALAAEHGLPLRVMHGDAFRRRYPWLGRAALGGSLCETDGHANPRIVSPAFARA
ARAAGADVFEHTPVDDVRHDGTRFRIHAAGRACTSTWLVNAAGAWANAIAERFGEAVPMEPIYPNMWVTEPLPPFIEHNL
GVYGGGVYARQVARGNCVIGGGRGRGDGEFGQPSVDTTRAVMRDACALLPALRDALLIRTWSGVEGCTPDHNPIVGASRT
TPKLLHAFGFSGGGFLLAPGVGDVLADLVTTGETVTPLDAFSIGRFAPQAEPTIPCRQQETQR
>Mature_382_residues
SETGHYDVAVVGGGLVGASAALALARRGLRVALFERRDCGAQASGVNYGGVRCQGRPAEQLPLALRARRIWDRLPERIGI
DGEFVVSGHLRLARSDADLEALHAYAALAAEHGLPLRVMHGDAFRRRYPWLGRAALGGSLCETDGHANPRIVSPAFARAA
RAAGADVFEHTPVDDVRHDGTRFRIHAAGRACTSTWLVNAAGAWANAIAERFGEAVPMEPIYPNMWVTEPLPPFIEHNLG
VYGGGVYARQVARGNCVIGGGRGRGDGEFGQPSVDTTRAVMRDACALLPALRDALLIRTWSGVEGCTPDHNPIVGASRTT
PKLLHAFGFSGGGFLLAPGVGDVLADLVTTGETVTPLDAFSIGRFAPQAEPTIPCRQQETQR

Specific function: Catalyzes the FAD-dependent oxidative deamination of various amines and D-amino acids to yield the corresponding alpha- keto acids, ammonia/amine, and hydrogen peroxide. Oxidizes sarcosine (N-methylglycine), N-ethylglycine and glycine. Can also oxidize th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DAO family [H]

Homologues:

Organism=Homo sapiens, GI197927446, Length=383, Percent_Identity=25.5874673629243, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI21361378, Length=383, Percent_Identity=25.5874673629243, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI24797151, Length=382, Percent_Identity=25.3926701570681, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI1787298, Length=384, Percent_Identity=23.9583333333333, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71994045, Length=382, Percent_Identity=25.3926701570681, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71994052, Length=389, Percent_Identity=25.1928020565553, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI20130091, Length=400, Percent_Identity=25.25, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076
- InterPro:   IPR012727 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.4.3.19 [H]

Molecular weight: Translated: 40731; Mature: 40599

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETGHYDVAVVGGGLVGASAALALARRGLRVALFERRDCGAQASGVNYGGVRCQGRPAE
CCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEECCCCHH
QLPLALRARRIWDRLPERIGIDGEFVVSGHLRLARSDADLEALHAYAALAAEHGLPLRVM
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEECCCCCHHHHHHHHHHHHHCCCEEEEE
HGDAFRRRYPWLGRAALGGSLCETDGHANPRIVSPAFARAARAAGADVFEHTPVDDVRHD
CCHHHHHHCCCCCHHHHCCCCCCCCCCCCCCEECHHHHHHHHHCCCHHHHCCCHHHHHCC
GTRFRIHAAGRACTSTWLVNAAGAWANAIAERFGEAVPMEPIYPNMWVTEPLPPFIEHNL
CCEEEEEECCCHHHHHHHEECCHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCHHHHCCC
GVYGGGVYARQVARGNCVIGGGRGRGDGEFGQPSVDTTRAVMRDACALLPALRDALLIRT
CEECCCHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEE
WSGVEGCTPDHNPIVGASRTTPKLLHAFGFSGGGFLLAPGVGDVLADLVTTGETVTPLDA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEECCCHHHHHHHHHHCCCCCCCCHH
FSIGRFAPQAEPTIPCRQQETQR
HHHCCCCCCCCCCCCCCCHHCCC
>Mature Secondary Structure 
SETGHYDVAVVGGGLVGASAALALARRGLRVALFERRDCGAQASGVNYGGVRCQGRPAE
CCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEECCCCHH
QLPLALRARRIWDRLPERIGIDGEFVVSGHLRLARSDADLEALHAYAALAAEHGLPLRVM
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEECCCCCHHHHHHHHHHHHHCCCEEEEE
HGDAFRRRYPWLGRAALGGSLCETDGHANPRIVSPAFARAARAAGADVFEHTPVDDVRHD
CCHHHHHHCCCCCHHHHCCCCCCCCCCCCCCEECHHHHHHHHHCCCHHHHCCCHHHHHCC
GTRFRIHAAGRACTSTWLVNAAGAWANAIAERFGEAVPMEPIYPNMWVTEPLPPFIEHNL
CCEEEEEECCCHHHHHHHEECCHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCHHHHCCC
GVYGGGVYARQVARGNCVIGGGRGRGDGEFGQPSVDTTRAVMRDACALLPALRDALLIRT
CEECCCHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEE
WSGVEGCTPDHNPIVGASRTTPKLLHAFGFSGGGFLLAPGVGDVLADLVTTGETVTPLDA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEECCCHHHHHHHHHHCCCCCCCCHH
FSIGRFAPQAEPTIPCRQQETQR
HHHCCCCCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 9827558; 11744710 [H]