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The map label for this gene is gcvA [H]

Identifier: 189352153

GI number: 189352153

Start: 181782

End: 182675

Strand: Reverse

Name: gcvA [H]

Synonym: BMULJ_03371

Alternate gene names: 189352153

Gene position: 182675-181782 (Counterclockwise)

Preceding gene: 189352157

Following gene: 189352149

Centisome position: 7.39

GC content: 69.57

Gene sequence:

>894_bases
ATGCCGCCTCGCATGTCCCGCCTGCCGCCGCTCAATGCGCTTCGCGCGTTCGAAGTGGCCGCGCGCCATCTGAATTTCCG
CGCAGCGGCCGACGAGATCGGCGTCACGCAGGGCGCGGTCGCGCAGCAAGTGCGCCATCTGGAGGACGTGCTGGGGCTGA
AGCTGTTCGAGCGCCTGCCGCGCGGGCTCGCGCTCACGCACGACGGCGCCGCTTATTTCTCGGACGTGCAGCGCGCGCTG
CATGCGATCGCCGACGCGACCGATCGGCTCGTGAAGCGCCGCGCGACGCTGACGATCAGCACGACGCCGTCGTTCGCATC
GAAGTGGCTGATCCCGCGGCTCGCGCAGTTCACCGACGCGCATCCGGATATCGACGTGCGCGTGATCGCCGATCAGCAGC
TCGCGACGTTCCGTCACGACGGCGTCGATCTCGCGATCCGTTACGGAAAGCCGCCCTTCGGCAAGCACTTCGCCGCGCAC
GCGCTGTTTCCGCTCGACGTCTGCGCGGTATGCAGCCCCGCGCTGCTCGCCGCGCCTGCGTCGCCGCGCGCGCTGGCCGG
GCACGTGCTGCTGCACGATGCGCACGACTTGTGGCCCGCGTTCATCGCGGCGCTGCCCGAGCCCGTCGACGTCGATCCGC
ACAAAGGGCTGCGTTTCAACCAGACGTCGCTCGCGATCGATGCGGCCGTCGCCGGACAAGGCATCGCGCTTGCGACCGAT
CCGCTCGTCGAACGCGACATCGCGGCCGGACGCCTGTGCAAGCCGTTCGATTTCGCGTTTCCGCTGTCGGTCGGGTTCTA
TCTCGTGTACCCGGCCGAGCGGCGCGACGACGACGCGATCGCGGTGATGCGCGACTGGATGATCGGCGCGGCCGCGTCGG
ATCCGCGCGACTGA

Upstream 100 bases:

>100_bases
GCCGCCCGCCGGCGCGCCTCGGTGGTAATGTAGGCGCATCGCGCAGCGATGTCGAACCAGTATTTCTGCGCGCCGCCAAT
AGATTTTCTACAGACACCCG

Downstream 100 bases:

>100_bases
ACGCGCGGCCGCGCCGTCGCGTCAGTTGTAGCCGAGCACGGCCGGCGCAGCGTCGGCCGCGTCGCCCGCGTCGGCCGGTT
CGGCGCCGAAACGGCCGAGC

Product: LysR family glycine cleavage system transcriptional activator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MPPRMSRLPPLNALRAFEVAARHLNFRAAADEIGVTQGAVAQQVRHLEDVLGLKLFERLPRGLALTHDGAAYFSDVQRAL
HAIADATDRLVKRRATLTISTTPSFASKWLIPRLAQFTDAHPDIDVRVIADQQLATFRHDGVDLAIRYGKPPFGKHFAAH
ALFPLDVCAVCSPALLAAPASPRALAGHVLLHDAHDLWPAFIAALPEPVDVDPHKGLRFNQTSLAIDAAVAGQGIALATD
PLVERDIAAGRLCKPFDFAFPLSVGFYLVYPAERRDDDAIAVMRDWMIGAAASDPRD

Sequences:

>Translated_297_residues
MPPRMSRLPPLNALRAFEVAARHLNFRAAADEIGVTQGAVAQQVRHLEDVLGLKLFERLPRGLALTHDGAAYFSDVQRAL
HAIADATDRLVKRRATLTISTTPSFASKWLIPRLAQFTDAHPDIDVRVIADQQLATFRHDGVDLAIRYGKPPFGKHFAAH
ALFPLDVCAVCSPALLAAPASPRALAGHVLLHDAHDLWPAFIAALPEPVDVDPHKGLRFNQTSLAIDAAVAGQGIALATD
PLVERDIAAGRLCKPFDFAFPLSVGFYLVYPAERRDDDAIAVMRDWMIGAAASDPRD
>Mature_296_residues
PPRMSRLPPLNALRAFEVAARHLNFRAAADEIGVTQGAVAQQVRHLEDVLGLKLFERLPRGLALTHDGAAYFSDVQRALH
AIADATDRLVKRRATLTISTTPSFASKWLIPRLAQFTDAHPDIDVRVIADQQLATFRHDGVDLAIRYGKPPFGKHFAAHA
LFPLDVCAVCSPALLAAPASPRALAGHVLLHDAHDLWPAFIAALPEPVDVDPHKGLRFNQTSLAIDAAVAGQGIALATDP
LVERDIAAGRLCKPFDFAFPLSVGFYLVYPAERRDDDAIAVMRDWMIGAAASDPRD

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=295, Percent_Identity=40.6779661016949, Blast_Score=204, Evalue=7e-54,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=33.9100346020761, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1788706, Length=275, Percent_Identity=30.1818181818182, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI145693193, Length=299, Percent_Identity=27.7591973244147, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1787589, Length=287, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1786401, Length=262, Percent_Identity=26.3358778625954, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87081978, Length=262, Percent_Identity=27.8625954198473, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1787128, Length=264, Percent_Identity=24.2424242424242, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1789639, Length=253, Percent_Identity=25.6916996047431, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI157672245, Length=122, Percent_Identity=33.6065573770492, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32286; Mature: 32155

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPRMSRLPPLNALRAFEVAARHLNFRAAADEIGVTQGAVAQQVRHLEDVLGLKLFERLP
CCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
RGLALTHDGAAYFSDVQRALHAIADATDRLVKRRATLTISTTPSFASKWLIPRLAQFTDA
CCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHCCC
HPDIDVRVIADQQLATFRHDGVDLAIRYGKPPFGKHFAAHALFPLDVCAVCSPALLAAPA
CCCCEEEEEECCHHHHHHCCCEEEEEEECCCCCCCHHHHHHCCHHHHHHHCCCHHHCCCC
SPRALAGHVLLHDAHDLWPAFIAALPEPVDVDPHKGLRFNQTSLAIDAAVAGQGIALATD
CCHHHCCCEEEECHHHHHHHHHHHCCCCCCCCCCCCCEECCHHHEEEHHHCCCCEEEECC
PLVERDIAAGRLCKPFDFAFPLSVGFYLVYPAERRDDDAIAVMRDWMIGAAASDPRD
CHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PPRMSRLPPLNALRAFEVAARHLNFRAAADEIGVTQGAVAQQVRHLEDVLGLKLFERLP
CCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
RGLALTHDGAAYFSDVQRALHAIADATDRLVKRRATLTISTTPSFASKWLIPRLAQFTDA
CCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHCCC
HPDIDVRVIADQQLATFRHDGVDLAIRYGKPPFGKHFAAHALFPLDVCAVCSPALLAAPA
CCCCEEEEEECCHHHHHHCCCEEEEEEECCCCCCCHHHHHHCCHHHHHHHCCCHHHCCCC
SPRALAGHVLLHDAHDLWPAFIAALPEPVDVDPHKGLRFNQTSLAIDAAVAGQGIALATD
CCHHHCCCEEEECHHHHHHHHHHHCCCCCCCCCCCCCEECCHHHEEEHHHCCCCEEEECC
PLVERDIAAGRLCKPFDFAFPLSVGFYLVYPAERRDDDAIAVMRDWMIGAAASDPRD
CHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]