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The map label for this gene is gcvA [H]

Identifier: 189352140

GI number: 189352140

Start: 167397

End: 168350

Strand: Reverse

Name: gcvA [H]

Synonym: BMULJ_03358

Alternate gene names: 189352140

Gene position: 168350-167397 (Counterclockwise)

Preceding gene: 189352142

Following gene: 189352139

Centisome position: 6.81

GC content: 66.67

Gene sequence:

>954_bases
ATGCGCAAATTCAAGATCCCGAACATGGGCGCGCTCGTGGCCTTCGAAGCGGCCGCGCGTCATGAAAGCTTCACGCACGC
GGCCAAGGAACTGTTCCTGACCGAAAGCGCCGTCTCGCGGCAGATCGCGACGCTCGAATCGAGCCTCGGCGTGCGGCTGT
TCGCACGCGTGAAGCAGCGCGTCGTGCTCACGCGAGCGGGCAAGCTCTACGGCACGCAGGTGCGGCGCGCGCTCGAGGCG
CTCGACCGCGACACGCTATCGATCATCGCGCACGGCAGCGGCGGCGGGTATCTGGAACTCGCCGTGCTGCCGACGTTCGC
GTCGCACTGGCTGATTCCGCGCATCAAGAGCTTCTACGATCGCACGCCGGACGTGCGCGTCAACATGGGCAGCCGCACCG
ACCTGTTCTCGTTCGAGGACACCCATTTCGAAGCGGCGATCCACTACGGCAAGCCGACCTGGCCCGGCACGTCGTCCGAC
TATCTGTTCGGCGAGGAAGTCGTGCCGATCTGCTCGCCGGCGCTGCTCGACGGGCCCGTCACGCGCGTCGACGACCTGCT
CGCGTATCCGCTGCTGCACTCGACGACGCGCCCCGGCGCGTGGGCGCAATGGTTCGAGGCGCACGGCGTCGAGGACATCC
GCACGATGCAGGGCGTGCGCTACGAATTGCATACGATGCTGATCAGCGCGGCGGCGGCGGGCCTCGGCGTCGCGCTCGTG
CCGAAGTTCTTCGTCGAAGGACAGCTGCAGCAGCTCGGGCTCGTCGTACCCTGCGACGCCGCGACCGTGGGCGACTCGGC
CTACTACCTCGTGTATCCGACGGAGTTCAGTCACAGCAAGCCGCTGGAGTTGTTTCGTGCGTGGCTGCTCGAACAAGCGA
GCGCTTACGCGGCGCCGCGGCACGAAGCCGTGACGGTGGAGACCGATGCAGAGGATGACGAGGATGTCGAGTAA

Upstream 100 bases:

>100_bases
AAAGCGAGATTAAATTGACACGTGATGAGTTTTTGGAATTATTCGCGGGCCGCCGCCCTGCCGTTCGGGGCGCCTCCGTC
GACTAGACGAACCGAGCATC

Downstream 100 bases:

>100_bases
CGGCGCGATGGGCAGGCATCCGGACAAGCCGGCGCGCTCCTGGTCCGGCGCGCGCCGGGCCGGGTAACCCCGACCCGGCG
CTCCGGGCAGCCGACCTCGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MRKFKIPNMGALVAFEAAARHESFTHAAKELFLTESAVSRQIATLESSLGVRLFARVKQRVVLTRAGKLYGTQVRRALEA
LDRDTLSIIAHGSGGGYLELAVLPTFASHWLIPRIKSFYDRTPDVRVNMGSRTDLFSFEDTHFEAAIHYGKPTWPGTSSD
YLFGEEVVPICSPALLDGPVTRVDDLLAYPLLHSTTRPGAWAQWFEAHGVEDIRTMQGVRYELHTMLISAAAAGLGVALV
PKFFVEGQLQQLGLVVPCDAATVGDSAYYLVYPTEFSHSKPLELFRAWLLEQASAYAAPRHEAVTVETDAEDDEDVE

Sequences:

>Translated_317_residues
MRKFKIPNMGALVAFEAAARHESFTHAAKELFLTESAVSRQIATLESSLGVRLFARVKQRVVLTRAGKLYGTQVRRALEA
LDRDTLSIIAHGSGGGYLELAVLPTFASHWLIPRIKSFYDRTPDVRVNMGSRTDLFSFEDTHFEAAIHYGKPTWPGTSSD
YLFGEEVVPICSPALLDGPVTRVDDLLAYPLLHSTTRPGAWAQWFEAHGVEDIRTMQGVRYELHTMLISAAAAGLGVALV
PKFFVEGQLQQLGLVVPCDAATVGDSAYYLVYPTEFSHSKPLELFRAWLLEQASAYAAPRHEAVTVETDAEDDEDVE
>Mature_317_residues
MRKFKIPNMGALVAFEAAARHESFTHAAKELFLTESAVSRQIATLESSLGVRLFARVKQRVVLTRAGKLYGTQVRRALEA
LDRDTLSIIAHGSGGGYLELAVLPTFASHWLIPRIKSFYDRTPDVRVNMGSRTDLFSFEDTHFEAAIHYGKPTWPGTSSD
YLFGEEVVPICSPALLDGPVTRVDDLLAYPLLHSTTRPGAWAQWFEAHGVEDIRTMQGVRYELHTMLISAAAAGLGVALV
PKFFVEGQLQQLGLVVPCDAATVGDSAYYLVYPTEFSHSKPLELFRAWLLEQASAYAAPRHEAVTVETDAEDDEDVE

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=295, Percent_Identity=36.271186440678, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1786448, Length=288, Percent_Identity=32.6388888888889, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI1788706, Length=292, Percent_Identity=29.4520547945205, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=26.6211604095563, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI157672245, Length=118, Percent_Identity=38.9830508474576, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786401, Length=248, Percent_Identity=25.4032258064516, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787589, Length=296, Percent_Identity=23.9864864864865, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693105, Length=122, Percent_Identity=31.1475409836066, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35042; Mature: 35042

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKFKIPNMGALVAFEAAARHESFTHAAKELFLTESAVSRQIATLESSLGVRLFARVKQR
CCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
VVLTRAGKLYGTQVRRALEALDRDTLSIIAHGSGGGYLELAVLPTFASHWLIPRIKSFYD
HHHHHCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEEEHHHHHHHHHHHHHHHHC
RTPDVRVNMGSRTDLFSFEDTHFEAAIHYGKPTWPGTSSDYLFGEEVVPICSPALLDGPV
CCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHCCCHHCCCCH
TRVDDLLAYPLLHSTTRPGAWAQWFEAHGVEDIRTMQGVRYELHTMLISAAAAGLGVALV
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
PKFFVEGQLQQLGLVVPCDAATVGDSAYYLVYPTEFSHSKPLELFRAWLLEQASAYAAPR
HHHHHCCCHHHCCEEEECCCCEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCC
HEAVTVETDAEDDEDVE
CCEEEEECCCCCCCCCC
>Mature Secondary Structure
MRKFKIPNMGALVAFEAAARHESFTHAAKELFLTESAVSRQIATLESSLGVRLFARVKQR
CCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
VVLTRAGKLYGTQVRRALEALDRDTLSIIAHGSGGGYLELAVLPTFASHWLIPRIKSFYD
HHHHHCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEEEHHHHHHHHHHHHHHHHC
RTPDVRVNMGSRTDLFSFEDTHFEAAIHYGKPTWPGTSSDYLFGEEVVPICSPALLDGPV
CCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHCCCHHCCCCH
TRVDDLLAYPLLHSTTRPGAWAQWFEAHGVEDIRTMQGVRYELHTMLISAAAAGLGVALV
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
PKFFVEGQLQQLGLVVPCDAATVGDSAYYLVYPTEFSHSKPLELFRAWLLEQASAYAAPR
HHHHHCCCHHHCCEEEECCCCEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCC
HEAVTVETDAEDDEDVE
CCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]