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The map label for this gene is nodW [H]

Identifier: 189352115

GI number: 189352115

Start: 141797

End: 142438

Strand: Direct

Name: nodW [H]

Synonym: BMULJ_03333

Alternate gene names: 189352115

Gene position: 141797-142438 (Clockwise)

Preceding gene: 189352114

Following gene: 189352117

Centisome position: 5.73

GC content: 63.4

Gene sequence:

>642_bases
ATGTCAGATGCAGCGATAAAGGACGATTCGACGGGCGTCGTATACGTCGTTGACGACGATCCGGCCATGCGTGCGTCGCT
CGATACGCTGTTGCGCTCGATGGGGCTGCGCGTCGCGACGTTCGCGAGCACCGCGGAATTGCGCGCGGCGGAACGGGAGG
ACGTGTCGAGCTGCTTGCTGCTCGACGTTCGCTTGCGCGGCGAGAGCGGGCTCGTGTTCCAGCAGGCACACGACCGTCCG
CCGGTTCCGATCGTCGTCATCACGGGCTTTGCCGACGTCGAAGTGTGTCGGAAGGCATTGAAAGGCGGGGCGGTCGACTT
TCTGGTGAAACCCTTTACCGAGCAGGAACTGATCGACGCGGTCAATATCGCGTTGTCGCTCGATGCCGCGCGGCGCAGCC
GCAACGACACGCGCCAGCAGTTGCAGCAGTCGTTCGAGTTGCTCACGCGTCGCGAGCGCGAAGTCCTGGCCTACGTGGTG
AGCGGCGCACTGAACAAGCAGATCGCTTCGCACCTCGGATTGAGCCTGATTACGATCAAGCAGCATCGCGCGTCCGTGAT
GCGCAAGATGAATGCATCGTCGCTCGCGGATCTCGTGCGCAAGTCGGAGGCGATCGGCGTGCCGCTGTCGGCCGGTTGCT
AG

Upstream 100 bases:

>100_bases
GTGCTCGGCGTGCTTGGCACGCTCGAACGCGAGGCGCTGAGCGGCGCGATGAGGGTCGCGACGCCGAACGAAAACCGAAT
GGACGATTGGGACGCAGGAT

Downstream 100 bases:

>100_bases
CGGCAGCTCGTCACGCGGAGCGGCAATCCGCTTGCACGTGCGCGGTATGCGCTGCACGCGGGACGCCGCGCCGCGCTGCG
GTCATTCGGCCGGATCGGGC

Product: putative response regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MSDAAIKDDSTGVVYVVDDDPAMRASLDTLLRSMGLRVATFASTAELRAAEREDVSSCLLLDVRLRGESGLVFQQAHDRP
PVPIVVITGFADVEVCRKALKGGAVDFLVKPFTEQELIDAVNIALSLDAARRSRNDTRQQLQQSFELLTRREREVLAYVV
SGALNKQIASHLGLSLITIKQHRASVMRKMNASSLADLVRKSEAIGVPLSAGC

Sequences:

>Translated_213_residues
MSDAAIKDDSTGVVYVVDDDPAMRASLDTLLRSMGLRVATFASTAELRAAEREDVSSCLLLDVRLRGESGLVFQQAHDRP
PVPIVVITGFADVEVCRKALKGGAVDFLVKPFTEQELIDAVNIALSLDAARRSRNDTRQQLQQSFELLTRREREVLAYVV
SGALNKQIASHLGLSLITIKQHRASVMRKMNASSLADLVRKSEAIGVPLSAGC
>Mature_212_residues
SDAAIKDDSTGVVYVVDDDPAMRASLDTLLRSMGLRVATFASTAELRAAEREDVSSCLLLDVRLRGESGLVFQQAHDRPP
VPIVVITGFADVEVCRKALKGGAVDFLVKPFTEQELIDAVNIALSLDAARRSRNDTRQQLQQSFELLTRREREVLAYVVS
GALNKQIASHLGLSLITIKQHRASVMRKMNASSLADLVRKSEAIGVPLSAGC

Specific function: Member of the two-component regulatory system nodV/nodW probably involved in the regulation of the transcription of genes involved in the nodulation process [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=176, Percent_Identity=28.9772727272727, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR011990
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23162; Mature: 23031

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAAIKDDSTGVVYVVDDDPAMRASLDTLLRSMGLRVATFASTAELRAAEREDVSSCLL
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEE
LDVRLRGESGLVFQQAHDRPPVPIVVITGFADVEVCRKALKGGAVDFLVKPFTEQELIDA
EEEEECCCCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHCCCCEEEHCCCCCHHHHHHH
VNIALSLDAARRSRNDTRQQLQQSFELLTRREREVLAYVVSGALNKQIASHLGLSLITIK
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHH
QHRASVMRKMNASSLADLVRKSEAIGVPLSAGC
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SDAAIKDDSTGVVYVVDDDPAMRASLDTLLRSMGLRVATFASTAELRAAEREDVSSCLL
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEE
LDVRLRGESGLVFQQAHDRPPVPIVVITGFADVEVCRKALKGGAVDFLVKPFTEQELIDA
EEEEECCCCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHCCCCEEEHCCCCCHHHHHHH
VNIALSLDAARRSRNDTRQQLQQSFELLTRREREVLAYVVSGALNKQIASHLGLSLITIK
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHH
QHRASVMRKMNASSLADLVRKSEAIGVPLSAGC
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2320582; 11157954; 12597275 [H]