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The map label for this gene is folE [H]

Identifier: 189352013

GI number: 189352013

Start: 31793

End: 32422

Strand: Reverse

Name: folE [H]

Synonym: BMULJ_03230

Alternate gene names: 189352013

Gene position: 32422-31793 (Counterclockwise)

Preceding gene: 189352014

Following gene: 189352012

Centisome position: 1.31

GC content: 64.13

Gene sequence:

>630_bases
ATGGGAAAAAAAACCGCGCGGTCCGAGCCGGCCGCATCCCGCCCGAGTCGTGAAGAAGCCGAGGACGCCGTACGCGTCCT
GCTGCGCTGGGCCGGCGACGATCCGACGCGCGAAGGGCTGATCGATACGCCCGCGCGCGTCGTGCGCGCCTACGAGCAGT
TCTTCGCCGGCTATTCGATCGAGCCGCGCGACATCCTCGCGCGCACGTTCAGCGAAGTCGACGGCTACGACGAAATGATC
GTGCTGAAGGACATCCGCTTCGAGAGCTACTGCGAGCATCACATGGTGCCGATCATCGGCCGCGCGCACGTCGCGTATCT
GCCGAATCATCGTGTGGTCGGCATCTCGAAGCTCGCGCGCCTCGTCGATGCGTTCGCGAAGCGCCTGCAGATCCAGGAAA
AGATGACGGTGCAGATCGCCGACACGCTGTTCGATGTGCTGCAGCCGAAAGGCGTCGGCGTGATACTCGAAGCCGCGCAT
CAGTGCATTTCGACGCGCGGCGTGCACAAGCCGGGCGTCGAGATGGTCACGTCGCGGATGCTCGGCACGTTCCGCACCGA
TCCGTCGACGCGGCGCGAATTCCTGTCGATCGTCGCGAACCCGTCTTCCGTGAACCTGACGAATACGTAA

Upstream 100 bases:

>100_bases
GGCCGAAGTGAGCGCCGGGCCGCACGCACGAAGCGCGAAAGCATGAAACAATGCCCGGATACCGCGCCGCCGCAAACGGC
GCCTTCGATGGAGCAACGCT

Downstream 100 bases:

>100_bases
ATGCAGTCAACGAAGCAAGCGGCGGATGCGCCCGTCGTGCTGGTGACCGGCGCCGCGCGCCGTGCCGGCCGCGCATTCGC
CGAGTATTTCGCCGCGCACG

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MGKKTARSEPAASRPSREEAEDAVRVLLRWAGDDPTREGLIDTPARVVRAYEQFFAGYSIEPRDILARTFSEVDGYDEMI
VLKDIRFESYCEHHMVPIIGRAHVAYLPNHRVVGISKLARLVDAFAKRLQIQEKMTVQIADTLFDVLQPKGVGVILEAAH
QCISTRGVHKPGVEMVTSRMLGTFRTDPSTRREFLSIVANPSSVNLTNT

Sequences:

>Translated_209_residues
MGKKTARSEPAASRPSREEAEDAVRVLLRWAGDDPTREGLIDTPARVVRAYEQFFAGYSIEPRDILARTFSEVDGYDEMI
VLKDIRFESYCEHHMVPIIGRAHVAYLPNHRVVGISKLARLVDAFAKRLQIQEKMTVQIADTLFDVLQPKGVGVILEAAH
QCISTRGVHKPGVEMVTSRMLGTFRTDPSTRREFLSIVANPSSVNLTNT
>Mature_208_residues
GKKTARSEPAASRPSREEAEDAVRVLLRWAGDDPTREGLIDTPARVVRAYEQFFAGYSIEPRDILARTFSEVDGYDEMIV
LKDIRFESYCEHHMVPIIGRAHVAYLPNHRVVGISKLARLVDAFAKRLQIQEKMTVQIADTLFDVLQPKGVGVILEAAHQ
CISTRGVHKPGVEMVTSRMLGTFRTDPSTRREFLSIVANPSSVNLTNT

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=176, Percent_Identity=46.5909090909091, Blast_Score=177, Evalue=7e-45,
Organism=Homo sapiens, GI4503949, Length=176, Percent_Identity=46.5909090909091, Blast_Score=177, Evalue=7e-45,
Organism=Homo sapiens, GI66932970, Length=146, Percent_Identity=42.4657534246575, Blast_Score=134, Evalue=8e-32,
Organism=Homo sapiens, GI66932972, Length=136, Percent_Identity=44.8529411764706, Blast_Score=132, Evalue=2e-31,
Organism=Escherichia coli, GI1788476, Length=175, Percent_Identity=36, Blast_Score=113, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17560486, Length=177, Percent_Identity=44.0677966101695, Blast_Score=178, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6321706, Length=180, Percent_Identity=45, Blast_Score=175, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24656782, Length=188, Percent_Identity=45.2127659574468, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24656796, Length=188, Percent_Identity=45.2127659574468, Blast_Score=161, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24656791, Length=188, Percent_Identity=45.2127659574468, Blast_Score=160, Evalue=4e-40,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 23407; Mature: 23276

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKKTARSEPAASRPSREEAEDAVRVLLRWAGDDPTREGLIDTPARVVRAYEQFFAGYSI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
EPRDILARTFSEVDGYDEMIVLKDIRFESYCEHHMVPIIGRAHVAYLPNHRVVGISKLAR
CHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHCCCCHHCCCEEEECCCCCEECHHHHHH
LVDAFAKRLQIQEKMTVQIADTLFDVLQPKGVGVILEAAHQCISTRGVHKPGVEMVTSRM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHH
LGTFRTDPSTRREFLSIVANPSSVNLTNT
HHHHCCCHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
GKKTARSEPAASRPSREEAEDAVRVLLRWAGDDPTREGLIDTPARVVRAYEQFFAGYSI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
EPRDILARTFSEVDGYDEMIVLKDIRFESYCEHHMVPIIGRAHVAYLPNHRVVGISKLAR
CHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHCCCCHHCCCEEEECCCCCEECHHHHHH
LVDAFAKRLQIQEKMTVQIADTLFDVLQPKGVGVILEAAHQCISTRGVHKPGVEMVTSRM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHH
LGTFRTDPSTRREFLSIVANPSSVNLTNT
HHHHCCCHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]