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The map label for this gene is iciA [H]

Identifier: 189352006

GI number: 189352006

Start: 26110

End: 27003

Strand: Direct

Name: iciA [H]

Synonym: BMULJ_03223

Alternate gene names: 189352006

Gene position: 26110-27003 (Clockwise)

Preceding gene: 189352003

Following gene: 189352010

Centisome position: 1.06

GC content: 69.46

Gene sequence:

>894_bases
ATGCTCGACTATGCATTGCTCGACGCGCTGGCCGCGGTGATCCGGCACGGGTCGTTCGAACGCGCAGCCAAGGAACTGAA
CGTGACGCCATCGGCCGTATCGCAGCGCGTGAAACTGCTGGAGGAGCGCGTCGGCAGCGTGCTCGTGAAGCGCGGTCAGC
CGTGCGTCGCGACGACCTCGGGCGCGCTGCTGTGCCGGCATACCGAACGCGTGCAACTGCTCGAGGCCGAGCTGTCGGGC
CGGATGCCCGCGCTGCCGGGGCCGCTCGCGTCGACGTGGCCGACGCTGCGCGTGGCCGTCAACGACGACAGCGTCGCGAC
CTGGTTCGTCGACGCCGTCGCGCCGTTCTGCGCCGAACGCGAGACCTTGCTCGATCTGGTAATCGACGACCAGGACTATA
CGGCCGAGCGCATCCGCGACGGCAGCGTGCAGGGCGCGGTCACCGCGCAGGCCGAGCCGATCCAGGGCTGCCGCTCGACG
CGGCTCGGGCGCATCCGCTACCGCGCGGTCTGTTCGCCCGCGTTTCATGCGCGCTACTTCGGCGCGGGTATCACGCGCGA
CGCACTGCGCCGCGCGCCGTGCGTGATGTTCAATCCGAAGGACGGATTGCAGGCGCGTTTCATCCGCCGCGTGACGCGGG
CGGATCTCGATCCGCCGCAGCACTGGATACCGCACGTCGCGGGCTATCTGCGCGCCTGCGAAACCGGGCTGGGATGGGGA
ATGTGCCCGGACCGGATGGTCGACCGTCAACTGGCGGCGGGCGAGCTGGTCGACATGTCGGCCGGGCGCACCGTCGACAT
CGAGCTTTACTGGCAGAGCTGGCGCCTGTCGATCGGCTGGCTCGACGATTTCAGCGCGGCGCTGAAGGCGCGCGCCGCGC
TGTTTCTCGATTGA

Upstream 100 bases:

>100_bases
TGGTGGATGAGCAGTAGACGGATGGATCGGGTAGCGCTAGTCTGCGGGCATCGCTTGAATAAGAGAAGCTAAATCAGCTA
ATGTCGATTAAGGAACGCTA

Downstream 100 bases:

>100_bases
CGATGCGGCGGGGAAGGCGGGAGAGGGGCGCTAAGGCGGCGCGCAGTACAGGCGCCGCGCGTCGCACGATCACACGTGAT
GCAGGCCGTCGAAGTCGACG

Product: chromosome replication initiation inhibitor protein

Products: NA

Alternate protein names: OriC replication inhibitor [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MLDYALLDALAAVIRHGSFERAAKELNVTPSAVSQRVKLLEERVGSVLVKRGQPCVATTSGALLCRHTERVQLLEAELSG
RMPALPGPLASTWPTLRVAVNDDSVATWFVDAVAPFCAERETLLDLVIDDQDYTAERIRDGSVQGAVTAQAEPIQGCRST
RLGRIRYRAVCSPAFHARYFGAGITRDALRRAPCVMFNPKDGLQARFIRRVTRADLDPPQHWIPHVAGYLRACETGLGWG
MCPDRMVDRQLAAGELVDMSAGRTVDIELYWQSWRLSIGWLDDFSAALKARAALFLD

Sequences:

>Translated_297_residues
MLDYALLDALAAVIRHGSFERAAKELNVTPSAVSQRVKLLEERVGSVLVKRGQPCVATTSGALLCRHTERVQLLEAELSG
RMPALPGPLASTWPTLRVAVNDDSVATWFVDAVAPFCAERETLLDLVIDDQDYTAERIRDGSVQGAVTAQAEPIQGCRST
RLGRIRYRAVCSPAFHARYFGAGITRDALRRAPCVMFNPKDGLQARFIRRVTRADLDPPQHWIPHVAGYLRACETGLGWG
MCPDRMVDRQLAAGELVDMSAGRTVDIELYWQSWRLSIGWLDDFSAALKARAALFLD
>Mature_297_residues
MLDYALLDALAAVIRHGSFERAAKELNVTPSAVSQRVKLLEERVGSVLVKRGQPCVATTSGALLCRHTERVQLLEAELSG
RMPALPGPLASTWPTLRVAVNDDSVATWFVDAVAPFCAERETLLDLVIDDQDYTAERIRDGSVQGAVTAQAEPIQGCRST
RLGRIRYRAVCSPAFHARYFGAGITRDALRRAPCVMFNPKDGLQARFIRRVTRADLDPPQHWIPHVAGYLRACETGLGWG
MCPDRMVDRQLAAGELVDMSAGRTVDIELYWQSWRLSIGWLDDFSAALKARAALFLD

Specific function: Specific inhibitor of chromosomal initiation of replication in vitro. Binds the three 13-mers in the origin (oriC) to block initiation of replication [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789283, Length=275, Percent_Identity=37.8181818181818, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1786401, Length=295, Percent_Identity=26.1016949152542, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR017685
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32820; Mature: 32820

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDYALLDALAAVIRHGSFERAAKELNVTPSAVSQRVKLLEERVGSVLVKRGQPCVATTS
CCHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
GALLCRHTERVQLLEAELSGRMPALPGPLASTWPTLRVAVNDDSVATWFVDAVAPFCAER
CCEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCH
ETLLDLVIDDQDYTAERIRDGSVQGAVTAQAEPIQGCRSTRLGRIRYRAVCSPAFHARYF
HHHHHHEECCCCCHHHHHCCCCCCCEEECCCCHHHHHHHCCCCHHHHEECCCHHHHHHHH
GAGITRDALRRAPCVMFNPKDGLQARFIRRVTRADLDPPQHWIPHVAGYLRACETGLGWG
CCCCCHHHHHHCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCC
MCPDRMVDRQLAAGELVDMSAGRTVDIELYWQSWRLSIGWLDDFSAALKARAALFLD
CCHHHHHHHHHHCCCEEECCCCCEEEEEEEEEEEEEEEECHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDYALLDALAAVIRHGSFERAAKELNVTPSAVSQRVKLLEERVGSVLVKRGQPCVATTS
CCHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
GALLCRHTERVQLLEAELSGRMPALPGPLASTWPTLRVAVNDDSVATWFVDAVAPFCAER
CCEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCH
ETLLDLVIDDQDYTAERIRDGSVQGAVTAQAEPIQGCRSTRLGRIRYRAVCSPAFHARYF
HHHHHHEECCCCCHHHHHCCCCCCCEEECCCCHHHHHHHCCCCHHHHEECCCHHHHHHHH
GAGITRDALRRAPCVMFNPKDGLQARFIRRVTRADLDPPQHWIPHVAGYLRACETGLGWG
CCCCCHHHHHHCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCC
MCPDRMVDRQLAAGELVDMSAGRTVDIELYWQSWRLSIGWLDDFSAALKARAALFLD
CCHHHHHHHHHHCCCEEECCCCCEEEEEEEEEEEEEEEECHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA