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The map label for this gene is traD [H]

Identifier: 189184520

GI number: 189184520

Start: 1641810

End: 1642820

Strand: Direct

Name: traD [H]

Synonym: OTT_1613

Alternate gene names: 189184520

Gene position: 1641810-1642820 (Clockwise)

Preceding gene: 189184519

Following gene: 189184523

Centisome position: 81.72

GC content: 33.83

Gene sequence:

>1011_bases
TTGGAAAAAAATAGTGAACAATGGTTGCCTTGGAATGATTGTTTTGAAGCAGCTGATTTTCATGATATAGCGAGTAGTTT
TAGTAATTATACTCCTAAACTTGATGACTTTTTTGCTAAAAATGCTGAATTAGTCTTATCTGAAGCATTGAAGCTATATA
AGGATGATAAAGATATCATAAAACTAATTCATACAATCGTTTACTCTGATAATAGACAATTTGCAAAAGCTTTTAGAAAC
ACAGCTGTATCAGGTATTATAAGCGAAAGCGCGCTCGAAACTTCTGCAGGAATTCAGTCTACTCTTGGAAAGAATATTAC
TTCGCTACAATATTTAAAGCCTGGAGGTAGTTTTAGCATCAAGGAATGGTTTAGTAATTCGAATGAAACTGGCTGGCTAT
TTATCACAGCTAACCCAAGTCAAAGAGCTACTTTATGCCCACTTATTTCAGCCTGGATAAGCATAGCTATCAAGGCTTTG
ATGTGTAGAAATCCTAATCATGATAACAAAAACATGTGGTTTATACTCGATGAATTGCCAGCTCTACAAAAAGTTTCATC
TTTACCAGTTGCTTTAGCTGAAAGTAGAAAGTATGGAGGCTGCTTTGTTGCTGGCTTGCAAAACATTCATCAATTAGAAG
CAATATATGGGGCTGCTGAATGTGCTTCTATGCTGGATTTGTTTAATAGTAAATTTATTTTTCGAGTTAGTGATCAGGTT
ACAGCTTATAAGTCAGCATTAACACTAGGTGAGCAAGAAATTATTGAAACTCAAGAGAACTTGTCATATGGATCAAATAC
TATGCGAGATGGGGTAAATATGAATAATGTTGAGCGTAAAAAGATTTTAGTTATGCCATCTGAAATTATGAACCTACCAG
ACCTTACATGTTATGTAAAACTTGCTGGTAACTTTCCCATCACAAAACTAACTATGCAGCTACAAAACTTAAATACAGCT
TTTGTTTGTGAATATAAATTGCTCAAAAAACTTAAGTTAGTAGAGTATTAA

Upstream 100 bases:

>100_bases
CACAAATTCGATTACACAAAGATCGAGCAATAATGTAGACACTATTGAAGCTTTTACTGATAGATTTTTTGACCCTAAAT
GTGATAAACTGCTTAATCCT

Downstream 100 bases:

>100_bases
TTCGAAAAATTAATACTCTAGTTATTTTTTAAATTAATCACTTCTTCTTTTGACAATCCGGTATTTTCAGAAATAAATTC
AACTGAAAAGCCAGCTTTTA

Product: putative conjugative transfer protein TraD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MEKNSEQWLPWNDCFEAADFHDIASSFSNYTPKLDDFFAKNAELVLSEALKLYKDDKDIIKLIHTIVYSDNRQFAKAFRN
TAVSGIISESALETSAGIQSTLGKNITSLQYLKPGGSFSIKEWFSNSNETGWLFITANPSQRATLCPLISAWISIAIKAL
MCRNPNHDNKNMWFILDELPALQKVSSLPVALAESRKYGGCFVAGLQNIHQLEAIYGAAECASMLDLFNSKFIFRVSDQV
TAYKSALTLGEQEIIETQENLSYGSNTMRDGVNMNNVERKKILVMPSEIMNLPDLTCYVKLAGNFPITKLTMQLQNLNTA
FVCEYKLLKKLKLVEY

Sequences:

>Translated_336_residues
MEKNSEQWLPWNDCFEAADFHDIASSFSNYTPKLDDFFAKNAELVLSEALKLYKDDKDIIKLIHTIVYSDNRQFAKAFRN
TAVSGIISESALETSAGIQSTLGKNITSLQYLKPGGSFSIKEWFSNSNETGWLFITANPSQRATLCPLISAWISIAIKAL
MCRNPNHDNKNMWFILDELPALQKVSSLPVALAESRKYGGCFVAGLQNIHQLEAIYGAAECASMLDLFNSKFIFRVSDQV
TAYKSALTLGEQEIIETQENLSYGSNTMRDGVNMNNVERKKILVMPSEIMNLPDLTCYVKLAGNFPITKLTMQLQNLNTA
FVCEYKLLKKLKLVEY
>Mature_336_residues
MEKNSEQWLPWNDCFEAADFHDIASSFSNYTPKLDDFFAKNAELVLSEALKLYKDDKDIIKLIHTIVYSDNRQFAKAFRN
TAVSGIISESALETSAGIQSTLGKNITSLQYLKPGGSFSIKEWFSNSNETGWLFITANPSQRATLCPLISAWISIAIKAL
MCRNPNHDNKNMWFILDELPALQKVSSLPVALAESRKYGGCFVAGLQNIHQLEAIYGAAECASMLDLFNSKFIFRVSDQV
TAYKSALTLGEQEIIETQENLSYGSNTMRDGVNMNNVERKKILVMPSEIMNLPDLTCYVKLAGNFPITKLTMQLQNLNTA
FVCEYKLLKKLKLVEY

Specific function: TraD is a cell envelope protein. The function of traD is unknown but it is essential for DNA transfer. It may form or may be part of a non-specific pore for DNA export, or it may directly energize DNA transport [H]

COG id: COG3505

COG function: function code U; Type IV secretory pathway, VirD4 components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To traD of plasmid IncFII R100 [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014128
- InterPro:   IPR019476
- InterPro:   IPR022585 [H]

Pfam domain/function: PF12615 TraD_N; PF10412 TrwB_AAD_bind [H]

EC number: NA

Molecular weight: Translated: 37799; Mature: 37799

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKNSEQWLPWNDCFEAADFHDIASSFSNYTPKLDDFFAKNAELVLSEALKLYKDDKDII
CCCCCCCCCCHHHHHHHCHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHCCCHHHH
KLIHTIVYSDNRQFAKAFRNTAVSGIISESALETSAGIQSTLGKNITSLQYLKPGGSFSI
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCH
KEWFSNSNETGWLFITANPSQRATLCPLISAWISIAIKALMCRNPNHDNKNMWFILDELP
HHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
ALQKVSSLPVALAESRKYGGCFVAGLQNIHQLEAIYGAAECASMLDLFNSKFIFRVSDQV
HHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHH
TAYKSALTLGEQEIIETQENLSYGSNTMRDGVNMNNVERKKILVMPSEIMNLPDLTCYVK
HHHHHHHHCCHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCEEEEECHHHCCCCCCEEEEE
LAGNFPITKLTMQLQNLNTAFVCEYKLLKKLKLVEY
EECCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKNSEQWLPWNDCFEAADFHDIASSFSNYTPKLDDFFAKNAELVLSEALKLYKDDKDII
CCCCCCCCCCHHHHHHHCHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHCCCHHHH
KLIHTIVYSDNRQFAKAFRNTAVSGIISESALETSAGIQSTLGKNITSLQYLKPGGSFSI
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCH
KEWFSNSNETGWLFITANPSQRATLCPLISAWISIAIKALMCRNPNHDNKNMWFILDELP
HHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
ALQKVSSLPVALAESRKYGGCFVAGLQNIHQLEAIYGAAECASMLDLFNSKFIFRVSDQV
HHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHH
TAYKSALTLGEQEIIETQENLSYGSNTMRDGVNMNNVERKKILVMPSEIMNLPDLTCYVK
HHHHHHHHCCHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCEEEEECHHHCCCCCCEEEEE
LAGNFPITKLTMQLQNLNTAFVCEYKLLKKLKLVEY
EECCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2680768; 7915817; 10464182; 3323526; 2164585; 2163400; 1618779; 10498725 [H]