Click here to switch to the map view.

The map label for this gene is phrB [H]

Identifier: 189184449

GI number: 189184449

Start: 1570996

End: 1572513

Strand: Direct

Name: phrB [H]

Synonym: OTT_1542

Alternate gene names: 189184449

Gene position: 1570996-1572513 (Clockwise)

Preceding gene: 189184448

Following gene: 189184455

Centisome position: 78.2

GC content: 29.25

Gene sequence:

>1518_bases
ATGAATATGAAAAAGATATCTATAGTATGGCTACGTCGTAACTTAAGGCTAGAAGATAATAAGCCTTTTGCAGAAGCATT
AAAATCTTCAAAGAAAATCATACCTATTTTTATTTTTGATACTACTATTCTCCAAAATTTTTCTAATCCTTTAGATCGTC
GACTATCTTTTTTAGCAAATACTATTTACAACCTAAATAGCGAATTACAAGAATTAGAAGGTAACCTGTTGGTATTATAT
GGTAATTCAGTGGAAATCATTCCTAAGCTTATAGAGAAATTGAATATTCAAACAATTTATGCTGATGAGGAGTATGATCC
AGAGAATGTTATCCGTGATCAAAAAATAACCAATCTTTTAGAGTTTAACGGAAAATCTAAATTGGAACTAGTTTGTGATC
ATTTATTGCTAAAGCCTGGTTCTATTGTAACACACAAAAAGCAGTATTATATGAGATATAATCCATATAATACTGCTTTT
AAACAATATTTAAGGAAGAATTGTTCATGTATAGAAAGATCTAATTATAGATTAGATCATAGATTAGTATCATTGGAGAG
CATAGATTTATCTAGTTTAAATTTAAAAATTATTAACCTTAACCATGGTGCAAGTGAAATATTAAATCAAATTGGTTATA
CTTACCAAAAAGATAATATTTGGCAGCCAATAAAGGCAAAAAATATCTTGAATAATTTTATTCAGAACAACATAACTAGT
TATAGAACTAACAATACTAACCAAAACCTTTTATATCTTAACAATAGTAGTACTATTTCACCTTATTTAAGATTTGGATT
AGTCTCAATAAGAGAATGTTTTTCTAAGGCTATAGCAGCTGATAGCAAGGCTTTAATCACAGATGAAGCTATAGGTAAAA
CTGAGAAAGAAAAAGGCTGGATCGATGGATTAATTCACAGAGAATTCTATGCAAGTCTTCTTTTTAGATTTCCAAATACT
GTAAGTGAGGAATTTCAAGATAAATACCGTAATAATATACCTTGGGAGAAGGATTTAGAAATTTATTGTAAATTTACTGA
AGGCAAAACTGGTTATCCAATGATAGATGCATCAATTAAGCAATTATTAAGAGATGGTTGGATGCATAATCTTGCACGTA
CTATTGTTGCAAATTTCTTCACTAAGTATTTACTGCTTGATTGGCGTTTGGGAGAGCATTTTTTTTCACAATATTTAATG
GATTATGAACTTGCTTCAAATGTGGGTAGTTGGCAATGGGCTGCATCATGTGGTACCGATATGGAACATTTTGTCCGTAT
GTTTTGTCCTTTTGCTCAAGGAAGAAAATTTGATCACGAAGCAAAGTATATAAAAAAATACTTACCTAACCTTGAAAATG
TTTCTGCTAAGGTTATTCATGACGTGCATTTTTCAAAAAAATATCCTAATTACCTAACTCCAATAATAGATTATCAATTA
TCAAAAGATAAATCTAGTAACTTTTTTAAACAGATAAAAAAGTTAAAGAACCAGCATCAAACTTTACTTAGCAAGTAA

Upstream 100 bases:

>100_bases
CAATATATATTTTGTTACTTACAATGGGTTTGTAAGCAGAGAGATGGTAATAAGCAATAAATAGTATAGTAATGCAATAA
TGCTATATCATTAAAAGGTG

Downstream 100 bases:

>100_bases
ATCACAATTATATAAAGTTATTATTCTATTTTTTTTAGAAAATCTTGAACATCTAAAGCTGCCATACAGCCGCTAGATGC
TGCTGTTACTGCTTGTCTAT

Product: deoxyribodipyrimidine photolyase, DNA photolyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MNMKKISIVWLRRNLRLEDNKPFAEALKSSKKIIPIFIFDTTILQNFSNPLDRRLSFLANTIYNLNSELQELEGNLLVLY
GNSVEIIPKLIEKLNIQTIYADEEYDPENVIRDQKITNLLEFNGKSKLELVCDHLLLKPGSIVTHKKQYYMRYNPYNTAF
KQYLRKNCSCIERSNYRLDHRLVSLESIDLSSLNLKIINLNHGASEILNQIGYTYQKDNIWQPIKAKNILNNFIQNNITS
YRTNNTNQNLLYLNNSSTISPYLRFGLVSIRECFSKAIAADSKALITDEAIGKTEKEKGWIDGLIHREFYASLLFRFPNT
VSEEFQDKYRNNIPWEKDLEIYCKFTEGKTGYPMIDASIKQLLRDGWMHNLARTIVANFFTKYLLLDWRLGEHFFSQYLM
DYELASNVGSWQWAASCGTDMEHFVRMFCPFAQGRKFDHEAKYIKKYLPNLENVSAKVIHDVHFSKKYPNYLTPIIDYQL
SKDKSSNFFKQIKKLKNQHQTLLSK

Sequences:

>Translated_505_residues
MNMKKISIVWLRRNLRLEDNKPFAEALKSSKKIIPIFIFDTTILQNFSNPLDRRLSFLANTIYNLNSELQELEGNLLVLY
GNSVEIIPKLIEKLNIQTIYADEEYDPENVIRDQKITNLLEFNGKSKLELVCDHLLLKPGSIVTHKKQYYMRYNPYNTAF
KQYLRKNCSCIERSNYRLDHRLVSLESIDLSSLNLKIINLNHGASEILNQIGYTYQKDNIWQPIKAKNILNNFIQNNITS
YRTNNTNQNLLYLNNSSTISPYLRFGLVSIRECFSKAIAADSKALITDEAIGKTEKEKGWIDGLIHREFYASLLFRFPNT
VSEEFQDKYRNNIPWEKDLEIYCKFTEGKTGYPMIDASIKQLLRDGWMHNLARTIVANFFTKYLLLDWRLGEHFFSQYLM
DYELASNVGSWQWAASCGTDMEHFVRMFCPFAQGRKFDHEAKYIKKYLPNLENVSAKVIHDVHFSKKYPNYLTPIIDYQL
SKDKSSNFFKQIKKLKNQHQTLLSK
>Mature_505_residues
MNMKKISIVWLRRNLRLEDNKPFAEALKSSKKIIPIFIFDTTILQNFSNPLDRRLSFLANTIYNLNSELQELEGNLLVLY
GNSVEIIPKLIEKLNIQTIYADEEYDPENVIRDQKITNLLEFNGKSKLELVCDHLLLKPGSIVTHKKQYYMRYNPYNTAF
KQYLRKNCSCIERSNYRLDHRLVSLESIDLSSLNLKIINLNHGASEILNQIGYTYQKDNIWQPIKAKNILNNFIQNNITS
YRTNNTNQNLLYLNNSSTISPYLRFGLVSIRECFSKAIAADSKALITDEAIGKTEKEKGWIDGLIHREFYASLLFRFPNT
VSEEFQDKYRNNIPWEKDLEIYCKFTEGKTGYPMIDASIKQLLRDGWMHNLARTIVANFFTKYLLLDWRLGEHFFSQYLM
DYELASNVGSWQWAASCGTDMEHFVRMFCPFAQGRKFDHEAKYIKKYLPNLENVSAKVIHDVHFSKKYPNYLTPIIDYQL
SKDKSSNFFKQIKKLKNQHQTLLSK

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI4758072, Length=479, Percent_Identity=27.1398747390397, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI188536100, Length=484, Percent_Identity=25.6198347107438, Blast_Score=160, Evalue=4e-39,
Organism=Homo sapiens, GI188536103, Length=434, Percent_Identity=25.5760368663594, Blast_Score=140, Evalue=4e-33,
Organism=Escherichia coli, GI1786926, Length=508, Percent_Identity=30.1181102362205, Blast_Score=214, Evalue=8e-57,
Organism=Saccharomyces cerevisiae, GI6324962, Length=507, Percent_Identity=24.4575936883629, Blast_Score=137, Evalue=3e-33,
Organism=Drosophila melanogaster, GI17137248, Length=484, Percent_Identity=26.4462809917355, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24585455, Length=484, Percent_Identity=26.4462809917355, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24648152, Length=225, Percent_Identity=31.1111111111111, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR019947
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 59284; Mature: 59284

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMKKISIVWLRRNLRLEDNKPFAEALKSSKKIIPIFIFDTTILQNFSNPLDRRLSFLAN
CCCCEEEEEEEECCCCCCCCCCHHHHHHCCCCEEEEEEEHHHHHHHCCCHHHHHHHHHHH
TIYNLNSELQELEGNLLVLYGNSVEIIPKLIEKLNIQTIYADEEYDPENVIRDQKITNLL
HHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHH
EFNGKSKLELVCDHLLLKPGSIVTHKKQYYMRYNPYNTAFKQYLRKNCSCIERSNYRLDH
HCCCCCHHHHHHHHHHCCCCCEEEECHHEEEEECCCHHHHHHHHHHCCCHHHCCCCCHHH
RLVSLESIDLSSLNLKIINLNHGASEILNQIGYTYQKDNIWQPIKAKNILNNFIQNNITS
HEEEEECCCCCEEEEEEEEECCCHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHCCCCE
YRTNNTNQNLLYLNNSSTISPYLRFGLVSIRECFSKAIAADSKALITDEAIGKTEKEKGW
EECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHCCCCHHCHH
IDGLIHREFYASLLFRFPNTVSEEFQDKYRNNIPWEKDLEIYCKFTEGKTGYPMIDASIK
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCHHHHHH
QLLRDGWMHNLARTIVANFFTKYLLLDWRLGEHFFSQYLMDYELASNVGSWQWAASCGTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHCCCC
MEHFVRMFCPFAQGRKFDHEAKYIKKYLPNLENVSAKVIHDVHFSKKYPNYLTPIIDYQL
HHHHHHHHCCHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHCHHHHHHC
SKDKSSNFFKQIKKLKNQHQTLLSK
CCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNMKKISIVWLRRNLRLEDNKPFAEALKSSKKIIPIFIFDTTILQNFSNPLDRRLSFLAN
CCCCEEEEEEEECCCCCCCCCCHHHHHHCCCCEEEEEEEHHHHHHHCCCHHHHHHHHHHH
TIYNLNSELQELEGNLLVLYGNSVEIIPKLIEKLNIQTIYADEEYDPENVIRDQKITNLL
HHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHH
EFNGKSKLELVCDHLLLKPGSIVTHKKQYYMRYNPYNTAFKQYLRKNCSCIERSNYRLDH
HCCCCCHHHHHHHHHHCCCCCEEEECHHEEEEECCCHHHHHHHHHHCCCHHHCCCCCHHH
RLVSLESIDLSSLNLKIINLNHGASEILNQIGYTYQKDNIWQPIKAKNILNNFIQNNITS
HEEEEECCCCCEEEEEEEEECCCHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHCCCCE
YRTNNTNQNLLYLNNSSTISPYLRFGLVSIRECFSKAIAADSKALITDEAIGKTEKEKGW
EECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHCCCCHHCHH
IDGLIHREFYASLLFRFPNTVSEEFQDKYRNNIPWEKDLEIYCKFTEGKTGYPMIDASIK
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCHHHHHH
QLLRDGWMHNLARTIVANFFTKYLLLDWRLGEHFFSQYLMDYELASNVGSWQWAASCGTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHCCCC
MEHFVRMFCPFAQGRKFDHEAKYIKKYLPNLENVSAKVIHDVHFSKKYPNYLTPIIDYQL
HHHHHHHHCCHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHCHHHHHHC
SKDKSSNFFKQIKKLKNQHQTLLSK
CCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2837735; 2110564; 9360600 [H]