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The map label for this gene is dnaB [H]

Identifier: 189184371

GI number: 189184371

Start: 1495966

End: 1497420

Strand: Direct

Name: dnaB [H]

Synonym: OTT_1464

Alternate gene names: 189184371

Gene position: 1495966-1497420 (Clockwise)

Preceding gene: 189184370

Following gene: 189184372

Centisome position: 74.46

GC content: 31.68

Gene sequence:

>1455_bases
ATGCCAGAGATCAAATTAAATCTAAGACCGAATCTTTTACGTCTATTTCGATATTTATCAGCAATAATTTTGAACTACCT
TAACCAGTTTCGAAAGATGTCTATTAACGAATTTTTACTGCCGGAACATTTTTATGAACCATTACATAGTAAAATATATA
AGTCAATTAATCTCATTATTAGTAAAGGAATTAGTGCTACTGTAATTTCGCTCAAAAATATGCTAGGCAATGAACTCGCA
TTTGAGGAAATAGGTGGAGTGGATTATCTAGCTAAACTTACAACGTTAGCATTAAGTATAGTTAATGTTAATGAGTACGG
CAAAATAGTATATGATCTTGCACTGCGGCGTTATTTAATTGAAATTGGAAAAAAAATAGTAACAAATGCATATTCTTCTA
CTTTAGCAGATTTAGCTATAACTCAGATCGAAACTGCTGAATCTCAATTATATGATCTAGGTTCAAGAGGAACTTTAAGT
AAAGGATTTACAAAATTACAAACTTCAATTGAAGAATCATGGACATCAATTTCATCTGCTATTAAAAATAAAAACTCTAT
TAACGGTATTAGTAGTGGACTACTTGACCTTGATTCAAAGCTTGGAGGATTTAAAAATTCTGACCTAATAATATTAGCTG
GCAGACCTTCAATGGGTAAAACTGCTTTAGGAGTTAACTTAGCAATAAATGCTTGTAAATATTTTTTATCATCATCTACT
CAACAAAATAGCAAAGTTTCTAATATAACACCATCAGTTGGATTCTTTTCTTTAGAAATGTCATCTCAGCAAATCTCAAC
TCGAATTCTTTCTATAGAATCAGAAATTAACAGCTCTGCATTATTTAACGGTAAAATAGATGAACAAGATGTTGATAAGT
TGAAGACTGTACAAGATGAAATACAAAAGTGGAATTTTTTTATAGATGATGCTCCAGCAATCTCGATATCTGCAATTAGA
TCTCGAGCTCGTAGACTTAAACGTACTCATAATTTAGCAATATTATTTATTAATTATTTACAGCTAATAAAAATTGATTC
CAGAGGAAGTCAGTATAATCGAGTACAGGAGATTTCTGAAATTACTCAGAGCTTAAAAGCTCTTGCTAAAGAGCTCAATA
TTTCAATAATTGCGTTATCTCAATTATCTATAGCTGTAGAACAAAGGTCAGATAAAAAGCCTATTCTCTCAGATCTACGA
GAATCAGGCTCCATTGAACAAGATGCCGATATTGTAATGCTCATATATCGGGACGAATATTACTTGTCTAGATCAGAACC
AGATCCAGGTACTCGAGAACACATAGAATGGAAAGCTAGACAAAATAAATGTTATAACACTGCTGAAATAATTGTTGCTA
AACACCGTAATGGGCCAGTTGGTACAGTGAAGTTGCACTATAATAGTAGGTATTCTAAATTTGGCAATATTGTTAAAAAC
TATCAGCAAGCTTAA

Upstream 100 bases:

>100_bases
GCAGAGCAAATGATACTTGGAGCAATTCTGATTAACAATCGTGCGCTATATAACATTAGATCTCTTTCGAAACTGGTTAA
AAGGTAGTTCAAAATTATAA

Downstream 100 bases:

>100_bases
TAAACACTAGATTATGCATAAATGAAAAAATGCTGGTCCAAAAAAAAAATTACAGCAAAATTTTACTCATTAACACCAGA
GATATGCAACTTAAGGTTAA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MPEIKLNLRPNLLRLFRYLSAIILNYLNQFRKMSINEFLLPEHFYEPLHSKIYKSINLIISKGISATVISLKNMLGNELA
FEEIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEIGKKIVTNAYSSTLADLAITQIETAESQLYDLGSRGTLS
KGFTKLQTSIEESWTSISSAIKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLSSST
QQNSKVSNITPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIDEQDVDKLKTVQDEIQKWNFFIDDAPAISISAIR
SRARRLKRTHNLAILFINYLQLIKIDSRGSQYNRVQEISEITQSLKALAKELNISIIALSQLSIAVEQRSDKKPILSDLR
ESGSIEQDADIVMLIYRDEYYLSRSEPDPGTREHIEWKARQNKCYNTAEIIVAKHRNGPVGTVKLHYNSRYSKFGNIVKN
YQQA

Sequences:

>Translated_484_residues
MPEIKLNLRPNLLRLFRYLSAIILNYLNQFRKMSINEFLLPEHFYEPLHSKIYKSINLIISKGISATVISLKNMLGNELA
FEEIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEIGKKIVTNAYSSTLADLAITQIETAESQLYDLGSRGTLS
KGFTKLQTSIEESWTSISSAIKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLSSST
QQNSKVSNITPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIDEQDVDKLKTVQDEIQKWNFFIDDAPAISISAIR
SRARRLKRTHNLAILFINYLQLIKIDSRGSQYNRVQEISEITQSLKALAKELNISIIALSQLSIAVEQRSDKKPILSDLR
ESGSIEQDADIVMLIYRDEYYLSRSEPDPGTREHIEWKARQNKCYNTAEIIVAKHRNGPVGTVKLHYNSRYSKFGNIVKN
YQQA
>Mature_483_residues
PEIKLNLRPNLLRLFRYLSAIILNYLNQFRKMSINEFLLPEHFYEPLHSKIYKSINLIISKGISATVISLKNMLGNELAF
EEIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEIGKKIVTNAYSSTLADLAITQIETAESQLYDLGSRGTLSK
GFTKLQTSIEESWTSISSAIKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLSSSTQ
QNSKVSNITPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIDEQDVDKLKTVQDEIQKWNFFIDDAPAISISAIRS
RARRLKRTHNLAILFINYLQLIKIDSRGSQYNRVQEISEITQSLKALAKELNISIIALSQLSIAVEQRSDKKPILSDLRE
SGSIEQDADIVMLIYRDEYYLSRSEPDPGTREHIEWKARQNKCYNTAEIIVAKHRNGPVGTVKLHYNSRYSKFGNIVKNY
QQA

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=457, Percent_Identity=35.0109409190372, Blast_Score=263, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 54402; Mature: 54271

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEIKLNLRPNLLRLFRYLSAIILNYLNQFRKMSINEFLLPEHFYEPLHSKIYKSINLII
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHH
SKGISATVISLKNMLGNELAFEEIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLI
HCCCHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHH
EIGKKIVTNAYSSTLADLAITQIETAESQLYDLGSRGTLSKGFTKLQTSIEESWTSISSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLSSST
HHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCC
QQNSKVSNITPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIDEQDVDKLKTVQDE
CCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHH
IQKWNFFIDDAPAISISAIRSRARRLKRTHNLAILFINYLQLIKIDSRGSQYNRVQEISE
HHHCCEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEECCCCCHHHHHHHHHH
ITQSLKALAKELNISIIALSQLSIAVEQRSDKKPILSDLRESGSIEQDADIVMLIYRDEY
HHHHHHHHHHHCCEEEEEEHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCE
YLSRSEPDPGTREHIEWKARQNKCYNTAEIIVAKHRNGPVGTVKLHYNSRYSKFGNIVKN
EECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEEEECCHHHHHHHHHHH
YQQA
HCCC
>Mature Secondary Structure 
PEIKLNLRPNLLRLFRYLSAIILNYLNQFRKMSINEFLLPEHFYEPLHSKIYKSINLII
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHH
SKGISATVISLKNMLGNELAFEEIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLI
HCCCHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHH
EIGKKIVTNAYSSTLADLAITQIETAESQLYDLGSRGTLSKGFTKLQTSIEESWTSISSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLSSST
HHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCC
QQNSKVSNITPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIDEQDVDKLKTVQDE
CCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHH
IQKWNFFIDDAPAISISAIRSRARRLKRTHNLAILFINYLQLIKIDSRGSQYNRVQEISE
HHHCCEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEECCCCCHHHHHHHHHH
ITQSLKALAKELNISIIALSQLSIAVEQRSDKKPILSDLRESGSIEQDADIVMLIYRDEY
HHHHHHHHHHHCCEEEEEEHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCE
YLSRSEPDPGTREHIEWKARQNKCYNTAEIIVAKHRNGPVGTVKLHYNSRYSKFGNIVKN
EECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEEEECCHHHHHHHHHHH
YQQA
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA