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The map label for this gene is nrdF [H]

Identifier: 189184364

GI number: 189184364

Start: 1490268

End: 1491347

Strand: Direct

Name: nrdF [H]

Synonym: OTT_1457

Alternate gene names: 189184364

Gene position: 1490268-1491347 (Clockwise)

Preceding gene: 189184363

Following gene: 189184365

Centisome position: 74.18

GC content: 31.57

Gene sequence:

>1080_bases
ATGAATGCTTGGCATGCCAGTAATTATAATTTAGGAAAGAGAACAATGTCACTACTTAATGCACGACCAATTTACAAGCC
ATTTTCATACCCTTGGGCATATAAAGCATGGCACACTCAGCAAAAAATTCACTGGCTACCAGAAGAAGTTCCATTAGCTG
ATGATATCAAAGATTGGAAATATAACTTAACTCCAGGAGAAAAGCATCTACTAACACAAATCTTTCGATTTTTTACTCAA
GCAGACATAGAGGTGAACAATTGTTATATGAAGCACTATGCTAGAGTTTTTCAACCAACTGAAGTACAAATGATGCTATC
TGCTTTTTCAAATATGGAAACTATTCATATAGCTGCATATTCACATCTGTTAGATACTATTGGCATGCCTGAAACGGAAT
ATCAGGCATTTATGAAATATAAAGCAATGAAGGATAAATATGATTACATGCAACGGTTTAATGTTGATAATAAAGAAGAA
ATTGCTACGACTTTAGCAGTTTTTGGAGCTTTCACAGAGGGGGTTCAACTATTTGCTTCATTTGCTATTTTACTAAATTT
TCCAAGATATAACAAAATGAAAGGAATGGGCCAAATTATTACATGGTCAGTTAGAGATGAAACATTACACACTAACTCTA
TTATTACGTTATTTAAAACCTTTATTCAAGAATATCCTGAAGTTTGGACAGAACAGTTAAAAAGTAGAATTTATGAGGCA
TGCTGTACTATTATTCATTTCGAAGATGCATTTATTGATTTAGCTTTTGAAGTTGGTGGAGTTGAAGGGTTAGAGGCTAG
AGATGTCAAAAAATATATTCGTTATATAGCTGACAGACGGTTATTACAACTTGGGTTAAAAGAAAATTATCTAATTGAAC
AAAACCCTTTAACATGGCTTGATCAAATCCTAAATGGAGTAGAACATACCAATTTCTTTGAAAATAGAGTTACTGAATAT
ACCAGAAGCGCTACTACTGGATCATGGGATGAAGTATTTGCTAAGATAGATAATAATAATAATAATAATAATAATAATAA
TAATAATAATAATAATAATAATAATCAGCCTACCGTATAA

Upstream 100 bases:

>100_bases
ACTTCTATTCAGCGCGCAGAAAAGGTTTCACATGTGGTTACAATTGATGAATTAGCAAAATTAACAGATAAAGAACAGCT
AGCAACAAGTAATAACGACG

Downstream 100 bases:

>100_bases
GTATTATGGCTAACATTCGCGATATTATTAGCACATTATTTGTTTTTATATAGCTGTATTCAGTCTCTTGGAACATAATA
TGTAATAACATGATCTATAG

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MNAWHASNYNLGKRTMSLLNARPIYKPFSYPWAYKAWHTQQKIHWLPEEVPLADDIKDWKYNLTPGEKHLLTQIFRFFTQ
ADIEVNNCYMKHYARVFQPTEVQMMLSAFSNMETIHIAAYSHLLDTIGMPETEYQAFMKYKAMKDKYDYMQRFNVDNKEE
IATTLAVFGAFTEGVQLFASFAILLNFPRYNKMKGMGQIITWSVRDETLHTNSIITLFKTFIQEYPEVWTEQLKSRIYEA
CCTIIHFEDAFIDLAFEVGGVEGLEARDVKKYIRYIADRRLLQLGLKENYLIEQNPLTWLDQILNGVEHTNFFENRVTEY
TRSATTGSWDEVFAKIDNNNNNNNNNNNNNNNNNNQPTV

Sequences:

>Translated_359_residues
MNAWHASNYNLGKRTMSLLNARPIYKPFSYPWAYKAWHTQQKIHWLPEEVPLADDIKDWKYNLTPGEKHLLTQIFRFFTQ
ADIEVNNCYMKHYARVFQPTEVQMMLSAFSNMETIHIAAYSHLLDTIGMPETEYQAFMKYKAMKDKYDYMQRFNVDNKEE
IATTLAVFGAFTEGVQLFASFAILLNFPRYNKMKGMGQIITWSVRDETLHTNSIITLFKTFIQEYPEVWTEQLKSRIYEA
CCTIIHFEDAFIDLAFEVGGVEGLEARDVKKYIRYIADRRLLQLGLKENYLIEQNPLTWLDQILNGVEHTNFFENRVTEY
TRSATTGSWDEVFAKIDNNNNNNNNNNNNNNNNNNQPTV
>Mature_359_residues
MNAWHASNYNLGKRTMSLLNARPIYKPFSYPWAYKAWHTQQKIHWLPEEVPLADDIKDWKYNLTPGEKHLLTQIFRFFTQ
ADIEVNNCYMKHYARVFQPTEVQMMLSAFSNMETIHIAAYSHLLDTIGMPETEYQAFMKYKAMKDKYDYMQRFNVDNKEE
IATTLAVFGAFTEGVQLFASFAILLNFPRYNKMKGMGQIITWSVRDETLHTNSIITLFKTFIQEYPEVWTEQLKSRIYEA
CCTIIHFEDAFIDLAFEVGGVEGLEARDVKKYIRYIADRRLLQLGLKENYLIEQNPLTWLDQILNGVEHTNFFENRVTEY
TRSATTGSWDEVFAKIDNNNNNNNNNNNNNNNNNNQPTV

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI42544136, Length=318, Percent_Identity=29.874213836478, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI289177074, Length=313, Percent_Identity=30.0319488817891, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI4557845, Length=310, Percent_Identity=29.0322580645161, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI260064013, Length=310, Percent_Identity=28.7096774193548, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI289177076, Length=292, Percent_Identity=29.7945205479452, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1789031, Length=309, Percent_Identity=24.5954692556634, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17551968, Length=286, Percent_Identity=29.020979020979, Blast_Score=109, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6322434, Length=309, Percent_Identity=27.1844660194175, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI18079279, Length=318, Percent_Identity=27.9874213836478, Blast_Score=105, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 42203; Mature: 42203

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAWHASNYNLGKRTMSLLNARPIYKPFSYPWAYKAWHTQQKIHWLPEEVPLADDIKDWK
CCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCHHHHCCCCCCCCCCCCCCCE
YNLTPGEKHLLTQIFRFFTQADIEVNNCYMKHYARVFQPTEVQMMLSAFSNMETIHIAAY
ECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEHHHHH
SHLLDTIGMPETEYQAFMKYKAMKDKYDYMQRFNVDNKEEIATTLAVFGAFTEGVQLFAS
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FAILLNFPRYNKMKGMGQIITWSVRDETLHTNSIITLFKTFIQEYPEVWTEQLKSRIYEA
HHHHHCCCCHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CCTIIHFEDAFIDLAFEVGGVEGLEARDVKKYIRYIADRRLLQLGLKENYLIEQNPLTWL
HHHHHEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHH
DQILNGVEHTNFFENRVTEYTRSATTGSWDEVFAKIDNNNNNNNNNNNNNNNNNNQPTV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNAWHASNYNLGKRTMSLLNARPIYKPFSYPWAYKAWHTQQKIHWLPEEVPLADDIKDWK
CCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCHHHHCCCCCCCCCCCCCCCE
YNLTPGEKHLLTQIFRFFTQADIEVNNCYMKHYARVFQPTEVQMMLSAFSNMETIHIAAY
ECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEHHHHH
SHLLDTIGMPETEYQAFMKYKAMKDKYDYMQRFNVDNKEEIATTLAVFGAFTEGVQLFAS
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FAILLNFPRYNKMKGMGQIITWSVRDETLHTNSIITLFKTFIQEYPEVWTEQLKSRIYEA
HHHHHCCCCHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CCTIIHFEDAFIDLAFEVGGVEGLEARDVKKYIRYIADRRLLQLGLKENYLIEQNPLTWL
HHHHHEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHH
DQILNGVEHTNFFENRVTEYTRSATTGSWDEVFAKIDNNNNNNNNNNNNNNNNNNQPTV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]