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The map label for this gene is truB [H]

Identifier: 189184269

GI number: 189184269

Start: 1391051

End: 1391959

Strand: Reverse

Name: truB [H]

Synonym: OTT_1362

Alternate gene names: 189184269

Gene position: 1391959-1391051 (Counterclockwise)

Preceding gene: 189184271

Following gene: 189184268

Centisome position: 69.29

GC content: 32.78

Gene sequence:

>909_bases
ATGAAAAGCAAAATTAATGGATGGATTAACTTATATAAGCCAGCTGGTATAAGTTCAGCACAAGCTGTAGCAAAAATTAA
ACACTGGCTCAATTTATCTAAAGTAGGGCATGCTGGTACACTTGATGTTGAAGCCGAAGGAGTGCTGCCAATAGCAATTG
GAGAAGCTACTAAGTTAGTTCAATATTTAATGGAAGCAACAAAAGAATATCATTTTACCATGAAGTTTGGCGCTAAAACT
GATAGCGGAGATGTATCAGGTAAGGTAGTGCAAGTAACTAGCAATATACCTACTTATGATGAATGTTTATCAGTAACAAA
GAAATTTATTGGTGAAATTGTTCAGGTTCCACCAAGCTATTCTGCATTAAAAATCAATGGTATTAGAGCATATAATCTTG
CTAGAAGTGGGCATAAAGTAATTCTTTCTCCTAGGAAAGTAAAAATTTTTAGCCTAAAATTGGAGTCTTATGATTCGATA
AACCTTACAGCAACATATTCAACTGAATGTTCAAAAGGTACTTATATCAGAACTTTAAGTCAAGATATAGCTTTATGCTT
GCAAAGCTTAAGTTTTGTGGTAAAATTAGCCCGTACTCGAGTAGGATGTTTTAACTATAAGCATGGCAGTCTTTATCTAA
ATGCATTGAATAGTTTTAGCTTCGCTAAAACTATAGCATGCCTTAAGGATGGTATTATGGATACTAAATGTGTATTAAGT
AACATTCCATCACTAAGTATAGATGATGTAGTGGCAAGGAAAATTTATTTCGGACAGCTAGTACAGTTGCCTAGATTACA
TAATTGCTCATTGGTATGGTTACAATATAATAATCAGCTAATTGCAATAGGAAAGATGGAGGATGGTTATTTTAGATCAT
CAAAAGTATTTAATTTTATAACTTTATAA

Upstream 100 bases:

>100_bases
TTTATTGCAATTTCACTTATTTTTATCATACAAGAAATGCTATTTTAGTTGCTAATATTCATAGATAATTATATAATTTA
TAATCATTAAATAACTTTAA

Downstream 100 bases:

>100_bases
GAAGATGTAAGGATTTTATTAATATGTCAATTACAGTTGAACGCAAAAAGGCACTAATTAGCGAATACGCAGATTTAGAA
AAAAACACTGGTTCAGTTGA

Product: tRNA pseudouridine 55 synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MKSKINGWINLYKPAGISSAQAVAKIKHWLNLSKVGHAGTLDVEAEGVLPIAIGEATKLVQYLMEATKEYHFTMKFGAKT
DSGDVSGKVVQVTSNIPTYDECLSVTKKFIGEIVQVPPSYSALKINGIRAYNLARSGHKVILSPRKVKIFSLKLESYDSI
NLTATYSTECSKGTYIRTLSQDIALCLQSLSFVVKLARTRVGCFNYKHGSLYLNALNSFSFAKTIACLKDGIMDTKCVLS
NIPSLSIDDVVARKIYFGQLVQLPRLHNCSLVWLQYNNQLIAIGKMEDGYFRSSKVFNFITL

Sequences:

>Translated_302_residues
MKSKINGWINLYKPAGISSAQAVAKIKHWLNLSKVGHAGTLDVEAEGVLPIAIGEATKLVQYLMEATKEYHFTMKFGAKT
DSGDVSGKVVQVTSNIPTYDECLSVTKKFIGEIVQVPPSYSALKINGIRAYNLARSGHKVILSPRKVKIFSLKLESYDSI
NLTATYSTECSKGTYIRTLSQDIALCLQSLSFVVKLARTRVGCFNYKHGSLYLNALNSFSFAKTIACLKDGIMDTKCVLS
NIPSLSIDDVVARKIYFGQLVQLPRLHNCSLVWLQYNNQLIAIGKMEDGYFRSSKVFNFITL
>Mature_302_residues
MKSKINGWINLYKPAGISSAQAVAKIKHWLNLSKVGHAGTLDVEAEGVLPIAIGEATKLVQYLMEATKEYHFTMKFGAKT
DSGDVSGKVVQVTSNIPTYDECLSVTKKFIGEIVQVPPSYSALKINGIRAYNLARSGHKVILSPRKVKIFSLKLESYDSI
NLTATYSTECSKGTYIRTLSQDIALCLQSLSFVVKLARTRVGCFNYKHGSLYLNALNSFSFAKTIACLKDGIMDTKCVLS
NIPSLSIDDVVARKIYFGQLVQLPRLHNCSLVWLQYNNQLIAIGKMEDGYFRSSKVFNFITL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=225, Percent_Identity=32.8888888888889, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI215599015, Length=263, Percent_Identity=26.615969581749, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4503337, Length=263, Percent_Identity=26.615969581749, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI2367200, Length=200, Percent_Identity=36.5, Blast_Score=132, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17553978, Length=200, Percent_Identity=28, Blast_Score=67, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6324037, Length=203, Percent_Identity=33.4975369458128, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323204, Length=266, Percent_Identity=25.187969924812, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281364189, Length=262, Percent_Identity=27.4809160305344, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI281364187, Length=262, Percent_Identity=27.4809160305344, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI281364185, Length=262, Percent_Identity=27.4809160305344, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI281364183, Length=262, Percent_Identity=27.4809160305344, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI62471759, Length=262, Percent_Identity=27.4809160305344, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI17975520, Length=262, Percent_Identity=27.4809160305344, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 33459; Mature: 33459

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSKINGWINLYKPAGISSAQAVAKIKHWLNLSKVGHAGTLDVEAEGVLPIAIGEATKLV
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCHHHHH
QYLMEATKEYHFTMKFGAKTDSGDVSGKVVQVTSNIPTYDECLSVTKKFIGEIVQVPPSY
HHHHHHHHCEEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCC
SALKINGIRAYNLARSGHKVILSPRKVKIFSLKLESYDSINLTATYSTECSKGTYIRTLS
CEEEECCEEEEEECCCCCEEEECCCEEEEEEEEECCCCCEEEEEEECCCCCCCCEEEHHH
QDIALCLQSLSFVVKLARTRVGCFNYKHGSLYLNALNSFSFAKTIACLKDGIMDTKCVLS
HHHHHHHHHHHHHHHHHHHHHCEEECCCCEEEEEECCCHHHHHHHHHHHCCCCCHHHHHH
NIPSLSIDDVVARKIYFGQLVQLPRLHNCSLVWLQYNNQLIAIGKMEDGYFRSSKVFNFI
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCEEEEEEECCCCCCCCCCCEEEE
TL
EC
>Mature Secondary Structure
MKSKINGWINLYKPAGISSAQAVAKIKHWLNLSKVGHAGTLDVEAEGVLPIAIGEATKLV
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCHHHHH
QYLMEATKEYHFTMKFGAKTDSGDVSGKVVQVTSNIPTYDECLSVTKKFIGEIVQVPPSY
HHHHHHHHCEEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCC
SALKINGIRAYNLARSGHKVILSPRKVKIFSLKLESYDSINLTATYSTECSKGTYIRTLS
CEEEECCEEEEEECCCCCEEEECCCEEEEEEEEECCCCCEEEEEEECCCCCCCCEEEHHH
QDIALCLQSLSFVVKLARTRVGCFNYKHGSLYLNALNSFSFAKTIACLKDGIMDTKCVLS
HHHHHHHHHHHHHHHHHHHHHCEEECCCCEEEEEECCCHHHHHHHHHHHCCCCCHHHHHH
NIPSLSIDDVVARKIYFGQLVQLPRLHNCSLVWLQYNNQLIAIGKMEDGYFRSSKVFNFI
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCEEEEEEECCCCCCCCCCCEEEE
TL
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA