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The map label for this gene is rpoH [H]

Identifier: 189184203

GI number: 189184203

Start: 1313831

End: 1314730

Strand: Direct

Name: rpoH [H]

Synonym: OTT_1296

Alternate gene names: 189184203

Gene position: 1313831-1314730 (Clockwise)

Preceding gene: 189184202

Following gene: 189184208

Centisome position: 65.4

GC content: 32.78

Gene sequence:

>900_bases
ATGGATAATGAAATAGATAATTTACCAATATCAGCTTCAGATACAGATAATGGATTTTATATTTACTTACAAAAGATTAA
TAGAATCCCTTCTTTGACTGCTGAGGAGGAATATATGCTTGCAAAGTCTTATTTTGAGCAAAATAACTTAAAAGCAGCGC
ATTCATTAGTCAAAAGCCATCTTAAGCTGGTTGCTAAAATAGCTTTGTCTTATCGTGGCTATGGTCTTCCAGTAGTTGAT
TTAGTTTCCGAGGGGAATATAGGGTTACTACAAGCAGTAAAAAAATATAATCCTGAACTTGGCTTTAGGTTGTCTACATA
TGCAATTTGGTGGATTAAAGCTGCAATACAGGATTATATTTTACGATCATGGTCTCTAGTAAAATTAGGAACAACAGCAG
CTCAAAAAAAACTGTTTTTTAATTTAGGTAAAATTAAAAATAAAATTACTAAGCTGCATGCAAGAGCTATTAATTCATCT
GATTATAAGCAAATTGCTGATGAGTCAGGAGTATCAATAGCAGAAGTATCAGAAGTTAATCAACGCTTATCAAACTCCGA
TCTATCGCTTAATACTCCTATTAGTTATCACGAAGATGATGCTACTGAATTAGTTGAATTGGTACCAGAGCATAGACCAA
ATCAAGAAATAATTTTAGCTAATCAGCAGGATTTAAACTTAAAACAAGAAGTTTTGCAAAATGCTTTACAAACTCTTACA
CAAAGAGAATTATATATTATTCGTGCACGAAAGCTACAAGATGAGCCTGATACGCTAGAAAAATTAAGTTTGCACTTTAA
TGTCTCCAAGGAGAGGATTCGCCAGATAGAAAGCAAAGCTTTTGAGAAGCTACAGCAGTATGCAATGAACACTAAGCATA
AGTTATTACATTTTGTTTAG

Upstream 100 bases:

>100_bases
CCTAAAGGTGGAAGATTTTATGAGCTACTAAAAGTTCAATATGTTGATTTTTAAAGTAATGCATTTAATTCATAGTTACA
TATAAAATAAAGAAATTGTT

Downstream 100 bases:

>100_bases
TAATGTAGAGTAAGGCTTGATTTTTTTGCTGTAGTATAATACTTCAATTTCATTATTACTATATAAGCTTTGATGCAAGA
TTTATACTTTATTACTTGAA

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MDNEIDNLPISASDTDNGFYIYLQKINRIPSLTAEEEYMLAKSYFEQNNLKAAHSLVKSHLKLVAKIALSYRGYGLPVVD
LVSEGNIGLLQAVKKYNPELGFRLSTYAIWWIKAAIQDYILRSWSLVKLGTTAAQKKLFFNLGKIKNKITKLHARAINSS
DYKQIADESGVSIAEVSEVNQRLSNSDLSLNTPISYHEDDATELVELVPEHRPNQEIILANQQDLNLKQEVLQNALQTLT
QRELYIIRARKLQDEPDTLEKLSLHFNVSKERIRQIESKAFEKLQQYAMNTKHKLLHFV

Sequences:

>Translated_299_residues
MDNEIDNLPISASDTDNGFYIYLQKINRIPSLTAEEEYMLAKSYFEQNNLKAAHSLVKSHLKLVAKIALSYRGYGLPVVD
LVSEGNIGLLQAVKKYNPELGFRLSTYAIWWIKAAIQDYILRSWSLVKLGTTAAQKKLFFNLGKIKNKITKLHARAINSS
DYKQIADESGVSIAEVSEVNQRLSNSDLSLNTPISYHEDDATELVELVPEHRPNQEIILANQQDLNLKQEVLQNALQTLT
QRELYIIRARKLQDEPDTLEKLSLHFNVSKERIRQIESKAFEKLQQYAMNTKHKLLHFV
>Mature_299_residues
MDNEIDNLPISASDTDNGFYIYLQKINRIPSLTAEEEYMLAKSYFEQNNLKAAHSLVKSHLKLVAKIALSYRGYGLPVVD
LVSEGNIGLLQAVKKYNPELGFRLSTYAIWWIKAAIQDYILRSWSLVKLGTTAAQKKLFFNLGKIKNKITKLHARAINSS
DYKQIADESGVSIAEVSEVNQRLSNSDLSLNTPISYHEDDATELVELVPEHRPNQEIILANQQDLNLKQEVLQNALQTLT
QRELYIIRARKLQDEPDTLEKLSLHFNVSKERIRQIESKAFEKLQQYAMNTKHKLLHFV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=277, Percent_Identity=38.2671480144404, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI1789098, Length=267, Percent_Identity=30.7116104868914, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1789448, Length=267, Percent_Identity=29.2134831460674, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34243; Mature: 34243

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNEIDNLPISASDTDNGFYIYLQKINRIPSLTAEEEYMLAKSYFEQNNLKAAHSLVKSH
CCCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHH
LKLVAKIALSYRGYGLPVVDLVSEGNIGLLQAVKKYNPELGFRLSTYAIWWIKAAIQDYI
HHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHH
LRSWSLVKLGTTAAQKKLFFNLGKIKNKITKLHARAINSSDYKQIADESGVSIAEVSEVN
HHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHH
QRLSNSDLSLNTPISYHEDDATELVELVPEHRPNQEIILANQQDLNLKQEVLQNALQTLT
HHHCCCCCEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
QRELYIIRARKLQDEPDTLEKLSLHFNVSKERIRQIESKAFEKLQQYAMNTKHKLLHFV
HHHHHEEEHHHCCCCCHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHCC
>Mature Secondary Structure
MDNEIDNLPISASDTDNGFYIYLQKINRIPSLTAEEEYMLAKSYFEQNNLKAAHSLVKSH
CCCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHH
LKLVAKIALSYRGYGLPVVDLVSEGNIGLLQAVKKYNPELGFRLSTYAIWWIKAAIQDYI
HHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHH
LRSWSLVKLGTTAAQKKLFFNLGKIKNKITKLHARAINSSDYKQIADESGVSIAEVSEVN
HHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHH
QRLSNSDLSLNTPISYHEDDATELVELVPEHRPNQEIILANQQDLNLKQEVLQNALQTLT
HHHCCCCCEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
QRELYIIRARKLQDEPDTLEKLSLHFNVSKERIRQIESKAFEKLQQYAMNTKHKLLHFV
HHHHHEEEHHHCCCCCHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606166 [H]