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The map label for this gene is traD [H]

Identifier: 189183999

GI number: 189183999

Start: 1122923

End: 1123909

Strand: Reverse

Name: traD [H]

Synonym: OTT_1092

Alternate gene names: 189183999

Gene position: 1123909-1122923 (Counterclockwise)

Preceding gene: 189184000

Following gene: 189183997

Centisome position: 55.94

GC content: 33.54

Gene sequence:

>987_bases
TTGCCTTGGAATGATTGTTTTGAAGCAGCTGATTTTCATGATATAGCTAGTAGTTTTAGTAATTATACCCATAAAATTGA
TGACTTTTTTACTAAAAATGCTGAATTAGTCTTGTCTGAAGCGTTGAAGCTATATAAGGATGATAAAGATATCATAAAAC
TAATTCATACAATCATTTACTCTGATAATAGACAATTTGCAAAAGCTTTTAGAAACACTGCTGTATCAGGTATTATAAGC
GAAAGCGCACTTGAAACTTCTGCAGGAATTCAATCTACTCTTGGAAAGAATATTACTTCGCTACAATATTTAAAGCCTGG
AGGTAGTTTTAGCATTAAGAAATGGTTTAGTAATTCTGCTGAAACTGGATGGTTATTTATAACAGCTAACCCAAGTCAAA
AAGCTACTTTATGCCCACTTATTTCAGCATGGATAAGCATAGCTATCAAGGCTTTGATGTACAGAAACCCTAATCATGAT
AACAAAAACATGTGGTTTATACTCGATGAACTGCCAGCTCTACAAAAAGTTTCGTCTTTACCAGTTGCTTTAGCTGAAAG
TAGAAAGTATGGAGGCTGCTTTGTTGCTGGATTGCAGAACATTCATCAATTAGAAGCAATATATGGTTCTGCTGAATGTG
CTTCTATGCTTGATTTGTTTAATAGTAAATTTATTTTTCGAGTTAGCGACCAGGTTACAGCTTATAAATCAGCATTAACA
CTAGGTGAGCAAGAGATAATTGAAACTCAAGAAAACTTGTCCTATGGCTCAAATACCATGCGAGATGGAGTAAATATGAA
TAATGTTGAGCGTAAAAAGCTCTTAGTTATGCCATCTGAAATTATGAATCTACCAGACCTTACATGTTATGTAAAGCTTG
CTGGTAACTTTCCCATCACAAAACTAACTATGCAGCTACAAAACTTAAATATAGCTTTTGTTTGGGGATATACATTGCTC
AAAAAACTTAAGTTAGTGGATTATTAA

Upstream 100 bases:

>100_bases
TAAGTTAGCAATTGGTGAACATTTTTATGATATCGATCAAATTTTGATTCTAAATGCGATAAACTGCTTAATCCTTTGGA
AAAAAAATAGTGAGCAATGG

Downstream 100 bases:

>100_bases
TTTAAAAGTTAATAATCCATGTTATTTTTTACATTAACCACTTCTTCTTTTGATAACCCAGTAGTTTCAGAAATAAATTC
AACTGAAAAGCCAGCTTTTA

Product: putative conjugative transfer protein TraD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MPWNDCFEAADFHDIASSFSNYTHKIDDFFTKNAELVLSEALKLYKDDKDIIKLIHTIIYSDNRQFAKAFRNTAVSGIIS
ESALETSAGIQSTLGKNITSLQYLKPGGSFSIKKWFSNSAETGWLFITANPSQKATLCPLISAWISIAIKALMYRNPNHD
NKNMWFILDELPALQKVSSLPVALAESRKYGGCFVAGLQNIHQLEAIYGSAECASMLDLFNSKFIFRVSDQVTAYKSALT
LGEQEIIETQENLSYGSNTMRDGVNMNNVERKKLLVMPSEIMNLPDLTCYVKLAGNFPITKLTMQLQNLNIAFVWGYTLL
KKLKLVDY

Sequences:

>Translated_328_residues
MPWNDCFEAADFHDIASSFSNYTHKIDDFFTKNAELVLSEALKLYKDDKDIIKLIHTIIYSDNRQFAKAFRNTAVSGIIS
ESALETSAGIQSTLGKNITSLQYLKPGGSFSIKKWFSNSAETGWLFITANPSQKATLCPLISAWISIAIKALMYRNPNHD
NKNMWFILDELPALQKVSSLPVALAESRKYGGCFVAGLQNIHQLEAIYGSAECASMLDLFNSKFIFRVSDQVTAYKSALT
LGEQEIIETQENLSYGSNTMRDGVNMNNVERKKLLVMPSEIMNLPDLTCYVKLAGNFPITKLTMQLQNLNIAFVWGYTLL
KKLKLVDY
>Mature_327_residues
PWNDCFEAADFHDIASSFSNYTHKIDDFFTKNAELVLSEALKLYKDDKDIIKLIHTIIYSDNRQFAKAFRNTAVSGIISE
SALETSAGIQSTLGKNITSLQYLKPGGSFSIKKWFSNSAETGWLFITANPSQKATLCPLISAWISIAIKALMYRNPNHDN
KNMWFILDELPALQKVSSLPVALAESRKYGGCFVAGLQNIHQLEAIYGSAECASMLDLFNSKFIFRVSDQVTAYKSALTL
GEQEIIETQENLSYGSNTMRDGVNMNNVERKKLLVMPSEIMNLPDLTCYVKLAGNFPITKLTMQLQNLNIAFVWGYTLLK
KLKLVDY

Specific function: TraD is a cell envelope protein. The function of traD is unknown but it is essential for DNA transfer. It may form or may be part of a non-specific pore for DNA export, or it may directly energize DNA transport [H]

COG id: COG3505

COG function: function code U; Type IV secretory pathway, VirD4 components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To traD of plasmid IncFII R100 [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014128
- InterPro:   IPR019476
- InterPro:   IPR022585 [H]

Pfam domain/function: PF12615 TraD_N; PF10412 TrwB_AAD_bind [H]

EC number: NA

Molecular weight: Translated: 36854; Mature: 36723

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPWNDCFEAADFHDIASSFSNYTHKIDDFFTKNAELVLSEALKLYKDDKDIIKLIHTIIY
CCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
SDNRQFAKAFRNTAVSGIISESALETSAGIQSTLGKNITSLQYLKPGGSFSIKKWFSNSA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCC
ETGWLFITANPSQKATLCPLISAWISIAIKALMYRNPNHDNKNMWFILDELPALQKVSSL
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHC
PVALAESRKYGGCFVAGLQNIHQLEAIYGSAECASMLDLFNSKFIFRVSDQVTAYKSALT
CHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHH
LGEQEIIETQENLSYGSNTMRDGVNMNNVERKKLLVMPSEIMNLPDLTCYVKLAGNFPIT
CCHHHHHHHHHHHCCCCCHHHCCCCCCCCCHHEEEEECHHHCCCCCCEEEEEEECCCCHH
KLTMQLQNLNIAFVWGYTLLKKLKLVDY
HHEEEHHCCCEEEEHHHHHHHHHHHCCC
>Mature Secondary Structure 
PWNDCFEAADFHDIASSFSNYTHKIDDFFTKNAELVLSEALKLYKDDKDIIKLIHTIIY
CCHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
SDNRQFAKAFRNTAVSGIISESALETSAGIQSTLGKNITSLQYLKPGGSFSIKKWFSNSA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCC
ETGWLFITANPSQKATLCPLISAWISIAIKALMYRNPNHDNKNMWFILDELPALQKVSSL
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHC
PVALAESRKYGGCFVAGLQNIHQLEAIYGSAECASMLDLFNSKFIFRVSDQVTAYKSALT
CHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHH
LGEQEIIETQENLSYGSNTMRDGVNMNNVERKKLLVMPSEIMNLPDLTCYVKLAGNFPIT
CCHHHHHHHHHHHCCCCCHHHCCCCCCCCCHHEEEEECHHHCCCCCCEEEEEEECCCCHH
KLTMQLQNLNIAFVWGYTLLKKLKLVDY
HHEEEHHCCCEEEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2680768; 7915817; 10464182; 3323526; 2164585; 2163400; 1618779; 10498725 [H]