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The map label for this gene is dnaB [H]

Identifier: 189183940

GI number: 189183940

Start: 1073521

End: 1074969

Strand: Reverse

Name: dnaB [H]

Synonym: OTT_1033

Alternate gene names: 189183940

Gene position: 1074969-1073521 (Counterclockwise)

Preceding gene: 189183941

Following gene: 189183939

Centisome position: 53.51

GC content: 32.16

Gene sequence:

>1449_bases
ATGGAAAAAGATTTAACTATTGCAAAGATTGCTCCAAGTAATATTCAGGCGGAGCAAATGATACTTGGGGCAATTCTGAT
TAACAATCGTGCGCTATATAACATTAACGAATTTTTACTGCCAGAACATTTTTACGAGCCATTACATGGTAAAATATATA
AGTCAATTAATCTCATTATTAGTAAAGGAATTAGTGCTACTGTAATTTCGCTCAAAAATATGCTAGGCAATGAACTCGCA
TTTGAGGAAATAGGTGGAGTAGATTATCTAGCTAAACTTACAACTTTAGCATTAAGTATAGTTAATGTTAATGAGTACGG
CAAAATAGTATATGATCTTGCGCTGCGGCGTTATTTAATTGAAATTGGAAAAAAAATAGTAACAAATGCATATTCTTCTA
CTTTAGCAGATTTAGCTATAACTCAGATCGAAACTGCTGAATCTCAATTATATGATCTAGGTTCAAGAGGAACTTTAAGT
AAAGGATTTACAAAATTACAAACTTCAATTGAAGAATCATGGACATCAATTTCATCTGCTATTAAAAATAAAAACTCTAT
TAACGGTATTAGTAGTGGACTACTTGACCTTGATTCAAAGCTTGGAGGATTTAAAAATTCTGACCTAATAATATTAGCTG
GCAGGCCATCAATGGGTAAAACTGCTTTAGGAGTTAACTTAGCAATAAATGCTTGTAAATATTTTTTATCATCATCTACT
CAACAAAATAGCAAAGTTTCTAATATAACACCATCAGTTGGATTCTTTTCTTTAGAAATGTCATCTCAGCAAATCTCAAC
TCGAATTCTTTCTATAGAATCTGAAATTAATAGCTCTGCATTATTTAATGGTAAAATAGGTGAACAAGATGTTGATAAGT
TAAAGACTGTACAAGACGAAATACAAAAGTGGAATTTTTTTGATGCTCCAGCAATCTCGATATCTGCAATTAGATCTCGA
GCTCGTAGACTTAAACGTACTCATAATTTAGCAATATTATTTATTAATTATTTACAGCTAATAAAAATTGATTCCAGAGG
AAGTCAGTATAATCGAGTACAGGAGATTTCTGAAATTACTCAGAGCTTAAAAGCTCTTGCTAAAGAGCTCAATATTTCAA
TAATTGCGTTATCTCAATTATCTATAGCTGTAGAACAAAGGTCAGATAAAAAGCCTATTCTCTCAGATCTACGAGAATCA
GGCTCCATTGAACAAGATGCCGATATTGTAATGCTCATATATCGGGACGAATATTACTTGTCTAGATCAGAACCAGATCC
AGGTACTCGAGAACACATAGAATGGAAAGCTAGACAAAATAAATGTTATAACACTGCTGAAATAATTGTTGCTAAACACC
GTAATGGGCCAGTTGGTACAGTGAAGTTGCACTATAATAGTAGGTATTCTAAATTTGGCAATATTGTTAAAAACTATCAG
CAAGCTTAA

Upstream 100 bases:

>100_bases
CAAGCAACCATATGGAAAATCTTGCGCACGCTTTTAAAGCTCAAGGATTTTCTTTTGAGTTAGTTAAGTTTAGTAATTTT
AATAAAATTTAAAGGGTGAT

Downstream 100 bases:

>100_bases
TAAACACTAGATTATGCATAAATGAAAAAATGCTGGTCCAAAAAAAAAATTACAGCAAAATTTTACTCATTAACACCAGA
GATATGCAACTTAAGGTTAA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MEKDLTIAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISATVISLKNMLGNELA
FEEIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEIGKKIVTNAYSSTLADLAITQIETAESQLYDLGSRGTLS
KGFTKLQTSIEESWTSISSAIKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLSSST
QQNSKVSNITPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIGEQDVDKLKTVQDEIQKWNFFDAPAISISAIRSR
ARRLKRTHNLAILFINYLQLIKIDSRGSQYNRVQEISEITQSLKALAKELNISIIALSQLSIAVEQRSDKKPILSDLRES
GSIEQDADIVMLIYRDEYYLSRSEPDPGTREHIEWKARQNKCYNTAEIIVAKHRNGPVGTVKLHYNSRYSKFGNIVKNYQ
QA

Sequences:

>Translated_482_residues
MEKDLTIAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISATVISLKNMLGNELA
FEEIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEIGKKIVTNAYSSTLADLAITQIETAESQLYDLGSRGTLS
KGFTKLQTSIEESWTSISSAIKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLSSST
QQNSKVSNITPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIGEQDVDKLKTVQDEIQKWNFFDAPAISISAIRSR
ARRLKRTHNLAILFINYLQLIKIDSRGSQYNRVQEISEITQSLKALAKELNISIIALSQLSIAVEQRSDKKPILSDLRES
GSIEQDADIVMLIYRDEYYLSRSEPDPGTREHIEWKARQNKCYNTAEIIVAKHRNGPVGTVKLHYNSRYSKFGNIVKNYQ
QA
>Mature_482_residues
MEKDLTIAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISATVISLKNMLGNELA
FEEIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEIGKKIVTNAYSSTLADLAITQIETAESQLYDLGSRGTLS
KGFTKLQTSIEESWTSISSAIKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLSSST
QQNSKVSNITPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIGEQDVDKLKTVQDEIQKWNFFDAPAISISAIRSR
ARRLKRTHNLAILFINYLQLIKIDSRGSQYNRVQEISEITQSLKALAKELNISIIALSQLSIAVEQRSDKKPILSDLRES
GSIEQDADIVMLIYRDEYYLSRSEPDPGTREHIEWKARQNKCYNTAEIIVAKHRNGPVGTVKLHYNSRYSKFGNIVKNYQ
QA

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=484, Percent_Identity=34.504132231405, Blast_Score=275, Evalue=7e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 53677; Mature: 53677

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKDLTIAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLII
CCCCCEEEEECCCCCCHHHHHHHHHEECCCEEECCHHHHCCHHHHCCHHHHHHHHHHHHH
SKGISATVISLKNMLGNELAFEEIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLI
HCCCHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHH
EIGKKIVTNAYSSTLADLAITQIETAESQLYDLGSRGTLSKGFTKLQTSIEESWTSISSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLSSST
HHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHCCCC
QQNSKVSNITPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIGEQDVDKLKTVQDE
CCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHH
IQKWNFFDAPAISISAIRSRARRLKRTHNLAILFINYLQLIKIDSRGSQYNRVQEISEIT
HHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEECCCCCHHHHHHHHHHHH
QSLKALAKELNISIIALSQLSIAVEQRSDKKPILSDLRESGSIEQDADIVMLIYRDEYYL
HHHHHHHHHCCEEEEEEEHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCEEE
SRSEPDPGTREHIEWKARQNKCYNTAEIIVAKHRNGPVGTVKLHYNSRYSKFGNIVKNYQ
CCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEEEECCHHHHHHHHHHHHC
QA
CC
>Mature Secondary Structure
MEKDLTIAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLII
CCCCCEEEEECCCCCCHHHHHHHHHEECCCEEECCHHHHCCHHHHCCHHHHHHHHHHHHH
SKGISATVISLKNMLGNELAFEEIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLI
HCCCHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHH
EIGKKIVTNAYSSTLADLAITQIETAESQLYDLGSRGTLSKGFTKLQTSIEESWTSISSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLSSST
HHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHCCCC
QQNSKVSNITPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIGEQDVDKLKTVQDE
CCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHH
IQKWNFFDAPAISISAIRSRARRLKRTHNLAILFINYLQLIKIDSRGSQYNRVQEISEIT
HHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEECCCCCHHHHHHHHHHHH
QSLKALAKELNISIIALSQLSIAVEQRSDKKPILSDLRESGSIEQDADIVMLIYRDEYYL
HHHHHHHHHCCEEEEEEEHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCEEE
SRSEPDPGTREHIEWKARQNKCYNTAEIIVAKHRNGPVGTVKLHYNSRYSKFGNIVKNYQ
CCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEEEECCHHHHHHHHHHHHC
QA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA