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The map label for this gene is recF [H]

Identifier: 189183858

GI number: 189183858

Start: 995380

End: 996516

Strand: Direct

Name: recF [H]

Synonym: OTT_0951

Alternate gene names: 189183858

Gene position: 995380-996516 (Clockwise)

Preceding gene: 189183857

Following gene: 189183860

Centisome position: 49.55

GC content: 29.73

Gene sequence:

>1137_bases
ATGTTAGGTACGAATTCAATATGTAGAATAACAAAGTTAGTACTACATAATTACCGCAATTTAACTGAATTAATAGTATC
TCCTCAATGTGATAAAATATTAATCATTGGTAAAAATGGTAGCGGTAAAACAAATTTGTTAGAATCAATATCATTATTTG
CTCCTGGGCGAGGATTACGTGGAGCTAAATATAGTGATATTTTAAGAAAGGAAGCGAACTTATCAAGTAATAGTAGTAAT
TGTCATAATGCTTATAGTCAATGGATAGCTGAAATCACATTACAAACAGCTATTAATATTGTAAAAATCTCGACTAATTA
CTATCAAAATACTACTAAGCGTAATATAAAGCTTAATGATAATACAATTACAAGCCATAAGTTGTTGGAGCTTGTGAATA
TGATCTGTATTACTCCGCAAATGGAAAGTGTTTTTCTTAATGGTGCTACTCAACGCAGAAAATTACTTGATAGAATAGTT
TATTTATTTGACTATAAACATGCTGAGCGTGTTAATAAATATGAGTATTACTTGCGAGAAAGAATGATTTTACTAAGAAG
TAATTCTAGCCAAACTAGATGGATTAATGTGATAGAAAATTGCTTAGCAAGTATATCACTAGAAATTGCTTCATGTCGCT
ATAATGCAATTAAGCAATTGCAACTATATCTTGATGAAATTGATGCTCCGTTTCCTAAAGTTAAACTCGATATTCAATGC
CAGATAGCAGAGCTATATTTGCAGCAATCGCCAAAACTATTAGAGTTGATTAATAGTAGATTTTGTAATTCTCGTACAAT
CGATTGTAACTCTGGAAAAAGCAACTTTGGAGTTCATCGTAGTGATTTTAAAGTAATTCATAGTGTCAAAAACCAACTAG
CTCAATTTTGTTCTACTGGTGAGCAAAAAGCTTTATTAATTTCACTACTGATTGCGCAAATGTTACAATCTAGAAAAAAT
TATAATAGGTTTCCAATACTACTATTAGATGAATTATTTATTCACTTAGACATAGAAAAAAGGCAATATTTAGCAAAATT
TTTAGCTCAATTTCCAGTTCAATGTTGGATTTCATCAACAGAGCTAGATGATGCAAATCTATTTAGTAATAATTGCGATA
TAGTTAATCTTCATTAA

Upstream 100 bases:

>100_bases
CTGATAAGTTTTTTAATCAAAAATATAAGGTCTTAGATTTACGAGACTCGTCAAAATATTATATTGAATATTATAATTAA
CAGAATTGTACATAATAATG

Downstream 100 bases:

>100_bases
TCATAGTTTGATATAACTCTAAATTATGATAATAGCAAGTTAGAAAGGTATAATATAATGGAACAAGCTACAAAAGCTTT
TGGTTTCTAGGTTATCTTTT

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MLGTNSICRITKLVLHNYRNLTELIVSPQCDKILIIGKNGSGKTNLLESISLFAPGRGLRGAKYSDILRKEANLSSNSSN
CHNAYSQWIAEITLQTAINIVKISTNYYQNTTKRNIKLNDNTITSHKLLELVNMICITPQMESVFLNGATQRRKLLDRIV
YLFDYKHAERVNKYEYYLRERMILLRSNSSQTRWINVIENCLASISLEIASCRYNAIKQLQLYLDEIDAPFPKVKLDIQC
QIAELYLQQSPKLLELINSRFCNSRTIDCNSGKSNFGVHRSDFKVIHSVKNQLAQFCSTGEQKALLISLLIAQMLQSRKN
YNRFPILLLDELFIHLDIEKRQYLAKFLAQFPVQCWISSTELDDANLFSNNCDIVNLH

Sequences:

>Translated_378_residues
MLGTNSICRITKLVLHNYRNLTELIVSPQCDKILIIGKNGSGKTNLLESISLFAPGRGLRGAKYSDILRKEANLSSNSSN
CHNAYSQWIAEITLQTAINIVKISTNYYQNTTKRNIKLNDNTITSHKLLELVNMICITPQMESVFLNGATQRRKLLDRIV
YLFDYKHAERVNKYEYYLRERMILLRSNSSQTRWINVIENCLASISLEIASCRYNAIKQLQLYLDEIDAPFPKVKLDIQC
QIAELYLQQSPKLLELINSRFCNSRTIDCNSGKSNFGVHRSDFKVIHSVKNQLAQFCSTGEQKALLISLLIAQMLQSRKN
YNRFPILLLDELFIHLDIEKRQYLAKFLAQFPVQCWISSTELDDANLFSNNCDIVNLH
>Mature_378_residues
MLGTNSICRITKLVLHNYRNLTELIVSPQCDKILIIGKNGSGKTNLLESISLFAPGRGLRGAKYSDILRKEANLSSNSSN
CHNAYSQWIAEITLQTAINIVKISTNYYQNTTKRNIKLNDNTITSHKLLELVNMICITPQMESVFLNGATQRRKLLDRIV
YLFDYKHAERVNKYEYYLRERMILLRSNSSQTRWINVIENCLASISLEIASCRYNAIKQLQLYLDEIDAPFPKVKLDIQC
QIAELYLQQSPKLLELINSRFCNSRTIDCNSGKSNFGVHRSDFKVIHSVKNQLAQFCSTGEQKALLISLLIAQMLQSRKN
YNRFPILLLDELFIHLDIEKRQYLAKFLAQFPVQCWISSTELDDANLFSNNCDIVNLH

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 43485; Mature: 43485

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS00024 HEMOPEXIN ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGTNSICRITKLVLHNYRNLTELIVSPQCDKILIIGKNGSGKTNLLESISLFAPGRGLR
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCC
GAKYSDILRKEANLSSNSSNCHNAYSQWIAEITLQTAINIVKISTNYYQNTTKRNIKLND
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCHHCCCCCCEEEECC
NTITSHKLLELVNMICITPQMESVFLNGATQRRKLLDRIVYLFDYKHAERVNKYEYYLRE
CCCHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RMILLRSNSSQTRWINVIENCLASISLEIASCRYNAIKQLQLYLDEIDAPFPKVKLDIQC
HHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
QIAELYLQQSPKLLELINSRFCNSRTIDCNSGKSNFGVHRSDFKVIHSVKNQLAQFCSTG
HHHHHHHHCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
EQKALLISLLIAQMLQSRKNYNRFPILLLDELFIHLDIEKRQYLAKFLAQFPVQCWISST
CHHHHHHHHHHHHHHHHHCCCCCCCHHEEEHHHHEECHHHHHHHHHHHHHCCHHEEEECC
ELDDANLFSNNCDIVNLH
CCCCCHHCCCCCCEEEEC
>Mature Secondary Structure
MLGTNSICRITKLVLHNYRNLTELIVSPQCDKILIIGKNGSGKTNLLESISLFAPGRGLR
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCC
GAKYSDILRKEANLSSNSSNCHNAYSQWIAEITLQTAINIVKISTNYYQNTTKRNIKLND
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCHHCCCCCCEEEECC
NTITSHKLLELVNMICITPQMESVFLNGATQRRKLLDRIVYLFDYKHAERVNKYEYYLRE
CCCHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RMILLRSNSSQTRWINVIENCLASISLEIASCRYNAIKQLQLYLDEIDAPFPKVKLDIQC
HHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
QIAELYLQQSPKLLELINSRFCNSRTIDCNSGKSNFGVHRSDFKVIHSVKNQLAQFCSTG
HHHHHHHHCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
EQKALLISLLIAQMLQSRKNYNRFPILLLDELFIHLDIEKRQYLAKFLAQFPVQCWISST
CHHHHHHHHHHHHHHHHHCCCCCCCHHEEEHHHHEECHHHHHHHHHHHHHCCHHEEEECC
ELDDANLFSNNCDIVNLH
CCCCCHHCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA