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The map label for this gene is phoB [H]

Identifier: 189183702

GI number: 189183702

Start: 839631

End: 840380

Strand: Reverse

Name: phoB [H]

Synonym: OTT_0795

Alternate gene names: 189183702

Gene position: 840380-839631 (Counterclockwise)

Preceding gene: 189183707

Following gene: 189183701

Centisome position: 41.83

GC content: 30.13

Gene sequence:

>750_bases
ATGTCTGCTTCTGAAAAGTTACCACCGCAAATTTTGGTAATCGAGCTAGATGAGATACAACGAATTGCTATTTGTAATAT
CATAGAAAGGTCACTATTTAATGTTATTAGATTAAGCAATATTAATGATATCGAAACAAGCAGCATCAACGCAGTGCTTA
TATTTGCTCCAAATGTAATTATTGTTAGCACCTCTATCAAAGAGGTTGTTCCAATTGATACATTTATTCAAAAGCTTAGA
ACTACCGCACAAAAATCTCATCGAATAGAAATACCTATAATTATTATTCTTAATCAAAACGATAATGTAGAAAATTATCA
AAAATTTGATAATGGTTTGATAGAAATTGTACAATATCCTTATACTGATTCTGAAATTATATTAGCAGTAAAGTCTTTGT
TACGCAGGTCTAGACCAATATTAAAAAACAGGCTGCTTAGATATAAAGACTTAACAATGGATCTGTCCAATTATAAAGTA
TATCGTGGTAATAAACCAATTCGTTTAGGTCCAACTGAATTTAAAATATTGCAGCTCTTATTAGAGTCACCTAAAGTTAT
ACTTTCACGTGAGAAAATTATAGATTATGTATGGGGTCATGGACATAAAATTGAGCTTAGAACAGTTGATGTACATGTTA
ATAGATTAAGGAATGTTATCAAAAACAGTAAATTTGAACCAGCTTTAATTAAAACTGTTCGAGCTTCAGGATATTGTTTA
AAATTACCAGGAGAATCAGATTACGCTTGA

Upstream 100 bases:

>100_bases
GTATCTTTAAAAGTACTTTAAAAGTACTTGATTTTTTAATCAAGGGTTGTATTATAATGACCTTATTGCAATAATTGTTT
AATAATTTGGTATAATACTT

Downstream 100 bases:

>100_bases
CAATACTGTAGTATATATAAATACATACTTTATATTATTTTATATAAATAACATAAGTTATTCATAAGTGTATTTATTGT
AATGTAACATAATAGGAATC

Product: response regulator PhoB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MSASEKLPPQILVIELDEIQRIAICNIIERSLFNVIRLSNINDIETSSINAVLIFAPNVIIVSTSIKEVVPIDTFIQKLR
TTAQKSHRIEIPIIIILNQNDNVENYQKFDNGLIEIVQYPYTDSEIILAVKSLLRRSRPILKNRLLRYKDLTMDLSNYKV
YRGNKPIRLGPTEFKILQLLLESPKVILSREKIIDYVWGHGHKIELRTVDVHVNRLRNVIKNSKFEPALIKTVRASGYCL
KLPGESDYA

Sequences:

>Translated_249_residues
MSASEKLPPQILVIELDEIQRIAICNIIERSLFNVIRLSNINDIETSSINAVLIFAPNVIIVSTSIKEVVPIDTFIQKLR
TTAQKSHRIEIPIIIILNQNDNVENYQKFDNGLIEIVQYPYTDSEIILAVKSLLRRSRPILKNRLLRYKDLTMDLSNYKV
YRGNKPIRLGPTEFKILQLLLESPKVILSREKIIDYVWGHGHKIELRTVDVHVNRLRNVIKNSKFEPALIKTVRASGYCL
KLPGESDYA
>Mature_248_residues
SASEKLPPQILVIELDEIQRIAICNIIERSLFNVIRLSNINDIETSSINAVLIFAPNVIIVSTSIKEVVPIDTFIQKLRT
TAQKSHRIEIPIIIILNQNDNVENYQKFDNGLIEIVQYPYTDSEIILAVKSLLRRSRPILKNRLLRYKDLTMDLSNYKVY
RGNKPIRLGPTEFKILQLLLESPKVILSREKIIDYVWGHGHKIELRTVDVHVNRLRNVIKNSKFEPALIKTVRASGYCLK
LPGESDYA

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=229, Percent_Identity=25.764192139738, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1786784, Length=124, Percent_Identity=36.2903225806452, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789809, Length=162, Percent_Identity=23.4567901234568, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1788394, Length=153, Percent_Identity=26.1437908496732, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87082012, Length=165, Percent_Identity=29.6969696969697, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1786911, Length=225, Percent_Identity=22.6666666666667, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790860, Length=152, Percent_Identity=25.6578947368421, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787229, Length=162, Percent_Identity=27.1604938271605, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 28642; Mature: 28511

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASEKLPPQILVIELDEIQRIAICNIIERSLFNVIRLSNINDIETSSINAVLIFAPNVI
CCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCEE
IVSTSIKEVVPIDTFIQKLRTTAQKSHRIEIPIIIILNQNDNVENYQKFDNGLIEIVQYP
EEECCHHHHCCHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCHHHHHHHCCCCEEEEECC
YTDSEIILAVKSLLRRSRPILKNRLLRYKDLTMDLSNYKVYRGNKPIRLGPTEFKILQLL
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCEEECCCEEEEECCCCEEECCHHHHHHHHH
LESPKVILSREKIIDYVWGHGHKIELRTVDVHVNRLRNVIKNSKFEPALIKTVRASGYCL
HHCCHHEEEHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHCCCCCHHHHHHHHCCCEEE
KLPGESDYA
ECCCCCCCC
>Mature Secondary Structure 
SASEKLPPQILVIELDEIQRIAICNIIERSLFNVIRLSNINDIETSSINAVLIFAPNVI
CCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCEE
IVSTSIKEVVPIDTFIQKLRTTAQKSHRIEIPIIIILNQNDNVENYQKFDNGLIEIVQYP
EEECCHHHHCCHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCHHHHHHHCCCCEEEEECC
YTDSEIILAVKSLLRRSRPILKNRLLRYKDLTMDLSNYKVYRGNKPIRLGPTEFKILQLL
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCEEECCCEEEEECCCCEEECCHHHHHHHHH
LESPKVILSREKIIDYVWGHGHKIELRTVDVHVNRLRNVIKNSKFEPALIKTVRASGYCL
HHCCHHEEEHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHCCCCCHHHHHHHHCCCEEE
KLPGESDYA
ECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]