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The map label for this gene is lipB [H]

Identifier: 189183579

GI number: 189183579

Start: 700553

End: 701179

Strand: Reverse

Name: lipB [H]

Synonym: OTT_0672

Alternate gene names: 189183579

Gene position: 701179-700553 (Counterclockwise)

Preceding gene: 189183584

Following gene: 189183577

Centisome position: 34.9

GC content: 30.94

Gene sequence:

>627_bases
ATGGTAGAGTGGATAGAAATTCAGTATCCTATCGAATATGGCGAAGCTTATAAAATGATGAAATCAAGATTAACAGGAAT
ACTAAACGGTACTGCTTCAGAAGCTGTATTTATTCTTGAGCATCAAGATGTTTATACTGCTGGAATTAGCGCTAAGAATG
ATGAATTATTAAATTGCTATGATATTCCAGTACATCATACTGATCGAGGAGGAAAGTTTACTTATCATGGTCCTGGGCAA
ATTATAATATATCCTGTTATCAATTTAGCAGTTAACGGAAGGGTAAAAGATATTAGAAATTATGTAAACAATCTTGCTTC
TCTTGTTATTAATTCTCTAAAGTTCTTTAATATTACAGGTATAACTGTTCAAGATACTATTGGAGTTTGGATAGATAGTG
AGTTTGGTAGAAAAAAAATAGCATCAATAGGAGTGCGAATACATAAGTGGATCACTTATCACGGAGTAGCTATTAATGTA
TGTCCAGATTTAAAAAAGTTTAAAGGAATAATTCCGTGTGGAGACCGTGACACTATTGTAACTTCAATTAGTGAATTACT
TGATCAAAAGATAGATCTAGATTACTATAAAGCTATATTAAAGCAAGAATTCTATAAGATATTTTAA

Upstream 100 bases:

>100_bases
TGAATATAGTCTAATCACGTTTTTTTAAATAAATTTAATTGTTTTTGATAATAGTTATGCATATTTTATATTATTATTTT
TTCTTAACAAAGTTTTTCTT

Downstream 100 bases:

>100_bases
TATAAGGTAGACTAAAATTTAACTCAAAAATTAAGAAATATATAAAGTATAATTGTGTGAGTAGACCTAACCAATCGCTT
AATTAGGCCCCTACGCGCAG

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MVEWIEIQYPIEYGEAYKMMKSRLTGILNGTASEAVFILEHQDVYTAGISAKNDELLNCYDIPVHHTDRGGKFTYHGPGQ
IIIYPVINLAVNGRVKDIRNYVNNLASLVINSLKFFNITGITVQDTIGVWIDSEFGRKKIASIGVRIHKWITYHGVAINV
CPDLKKFKGIIPCGDRDTIVTSISELLDQKIDLDYYKAILKQEFYKIF

Sequences:

>Translated_208_residues
MVEWIEIQYPIEYGEAYKMMKSRLTGILNGTASEAVFILEHQDVYTAGISAKNDELLNCYDIPVHHTDRGGKFTYHGPGQ
IIIYPVINLAVNGRVKDIRNYVNNLASLVINSLKFFNITGITVQDTIGVWIDSEFGRKKIASIGVRIHKWITYHGVAINV
CPDLKKFKGIIPCGDRDTIVTSISELLDQKIDLDYYKAILKQEFYKIF
>Mature_208_residues
MVEWIEIQYPIEYGEAYKMMKSRLTGILNGTASEAVFILEHQDVYTAGISAKNDELLNCYDIPVHHTDRGGKFTYHGPGQ
IIIYPVINLAVNGRVKDIRNYVNNLASLVINSLKFFNITGITVQDTIGVWIDSEFGRKKIASIGVRIHKWITYHGVAINV
CPDLKKFKGIIPCGDRDTIVTSISELLDQKIDLDYYKAILKQEFYKIF

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=192, Percent_Identity=32.2916666666667, Blast_Score=87, Evalue=9e-18,
Organism=Escherichia coli, GI87081767, Length=152, Percent_Identity=38.1578947368421, Blast_Score=105, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323268, Length=134, Percent_Identity=35.0746268656716, Blast_Score=84, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21355483, Length=194, Percent_Identity=33.5051546391753, Blast_Score=116, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 23649; Mature: 23649

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEWIEIQYPIEYGEAYKMMKSRLTGILNGTASEAVFILEHQDVYTAGISAKNDELLNCY
CCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEECCCCCCCCEEEEE
DIPVHHTDRGGKFTYHGPGQIIIYPVINLAVNGRVKDIRNYVNNLASLVINSLKFFNITG
ECCEEECCCCCEEEEECCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHEEEEEC
ITVQDTIGVWIDSEFGRKKIASIGVRIHKWITYHGVAINVCPDLKKFKGIIPCGDRDTIV
EEEECEEEEEEECCCCHHHHHHHHHHEEEEEEECCEEEEECCCHHHHCCCCCCCCCHHHH
TSISELLDQKIDLDYYKAILKQEFYKIF
HHHHHHHHHCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVEWIEIQYPIEYGEAYKMMKSRLTGILNGTASEAVFILEHQDVYTAGISAKNDELLNCY
CCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEECCCCCCCCEEEEE
DIPVHHTDRGGKFTYHGPGQIIIYPVINLAVNGRVKDIRNYVNNLASLVINSLKFFNITG
ECCEEECCCCCEEEEECCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHEEEEEC
ITVQDTIGVWIDSEFGRKKIASIGVRIHKWITYHGVAINVCPDLKKFKGIIPCGDRDTIV
EEEECEEEEEEECCCCHHHHHHHHHHEEEEEEECCEEEEECCCHHHHCCCCCCCCCHHHH
TSISELLDQKIDLDYYKAILKQEFYKIF
HHHHHHHHHCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA