Click here to switch to the map view.

The map label for this gene is ruvB [H]

Identifier: 189183541

GI number: 189183541

Start: 662655

End: 663632

Strand: Reverse

Name: ruvB [H]

Synonym: OTT_0634

Alternate gene names: 189183541

Gene position: 663632-662655 (Counterclockwise)

Preceding gene: 189183542

Following gene: 189183540

Centisome position: 33.03

GC content: 31.8

Gene sequence:

>978_bases
ATGAAAAATCAATTATTAGATGCTAAAGTAGCATCAGAAGAACAGGAGACAGTTTTACGACCTAGTTTGCTAAATGATTT
TACTGGACAAAATCAAATGAAGAGTAATTTGAAAATCTTTATTACGTCTTCAATTGAAAGAGATGAATCATTGGATCATA
CACTATTACATGGTCCGCCTGGATTAGGTAAAACTACCATTGCTCAAATTATTGCAAAAGAAAAAAATGTTAATTTAAAA
TCTACAGCTGGACCAATATTATCCAAAGCTGCTGATCTTGCTGCTATTTTAACTAATTTACAAAAAAATGATGTTTTATT
CATTGATGAAATTCATCGTCTAAACATTCATGTTGAAGAAATATTATATTCCGCTATGGAAGATTTTTCGCTTGATATTA
TGATTGGTGAAGGTCCATCTGCTAGGTCTGTAAAAATTTATTTACCAAAATTTACGTTAATAGGTGCTACAACTAGATTA
GGACTTATCAGCAAACCATTATGTGACCGATTTGGTATTCACCTAAAGCTTAATTTTTATAGTTGTGAAGAACTAACTAA
AATTGTTGAACGCGGAGCAAAAGTGTTAAACGTGGCTCTTGATACTAACGCAGCAATAGAGATTGCCAATCGTTCTCGTG
GTACTCCAAGAATAGCATTACGATTATTAAAGAGAGTACGAGATTTCGGAATTTATCAAAATATTAATCCATTAAATCAA
CAAATAACTGATTATGCTCTTAATCAGTTAGGAATTGATAAATTAGGATTAGATAGCAGCGATCATAAATATCTAAGATT
TATTGCTGAAAATTATGATGGTGGACCAGTAGGTATTGATACTATTGCTGCTGCTTTATCAGAACAACGAGATACTATAG
AAGAAATGATAGAACCTTACTTAATCCAAATTGGATTCGTGCAACGAACTCAGCGTGGTAGAGTTATTACTGCTAATGCT
CTTAAACATTTACATTAA

Upstream 100 bases:

>100_bases
GAGCTGAAGCAACTCAAATACTGTCTGATATTTATGCATTATCTCCTAGTATATCAGTTAATGAATTAGTTAGAACGGCC
TTGCAGCGAAGAGCTAAGTA

Downstream 100 bases:

>100_bases
ATTACATTTAAATCTTGACTATAACACATGCTATGCAGTATAAAATAATAATAGCAAAATCTCTTAACATAGTCTAGAAC
TGCGTTATTTTATAATGTTA

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MKNQLLDAKVASEEQETVLRPSLLNDFTGQNQMKSNLKIFITSSIERDESLDHTLLHGPPGLGKTTIAQIIAKEKNVNLK
STAGPILSKAADLAAILTNLQKNDVLFIDEIHRLNIHVEEILYSAMEDFSLDIMIGEGPSARSVKIYLPKFTLIGATTRL
GLISKPLCDRFGIHLKLNFYSCEELTKIVERGAKVLNVALDTNAAIEIANRSRGTPRIALRLLKRVRDFGIYQNINPLNQ
QITDYALNQLGIDKLGLDSSDHKYLRFIAENYDGGPVGIDTIAAALSEQRDTIEEMIEPYLIQIGFVQRTQRGRVITANA
LKHLH

Sequences:

>Translated_325_residues
MKNQLLDAKVASEEQETVLRPSLLNDFTGQNQMKSNLKIFITSSIERDESLDHTLLHGPPGLGKTTIAQIIAKEKNVNLK
STAGPILSKAADLAAILTNLQKNDVLFIDEIHRLNIHVEEILYSAMEDFSLDIMIGEGPSARSVKIYLPKFTLIGATTRL
GLISKPLCDRFGIHLKLNFYSCEELTKIVERGAKVLNVALDTNAAIEIANRSRGTPRIALRLLKRVRDFGIYQNINPLNQ
QITDYALNQLGIDKLGLDSSDHKYLRFIAENYDGGPVGIDTIAAALSEQRDTIEEMIEPYLIQIGFVQRTQRGRVITANA
LKHLH
>Mature_325_residues
MKNQLLDAKVASEEQETVLRPSLLNDFTGQNQMKSNLKIFITSSIERDESLDHTLLHGPPGLGKTTIAQIIAKEKNVNLK
STAGPILSKAADLAAILTNLQKNDVLFIDEIHRLNIHVEEILYSAMEDFSLDIMIGEGPSARSVKIYLPKFTLIGATTRL
GLISKPLCDRFGIHLKLNFYSCEELTKIVERGAKVLNVALDTNAAIEIANRSRGTPRIALRLLKRVRDFGIYQNINPLNQ
QITDYALNQLGIDKLGLDSSDHKYLRFIAENYDGGPVGIDTIAAALSEQRDTIEEMIEPYLIQIGFVQRTQRGRVITANA
LKHLH

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family [H]

Homologues:

Organism=Escherichia coli, GI1788167, Length=309, Percent_Identity=54.0453074433657, Blast_Score=350, Evalue=6e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- ProDom:   PD005323 [H]

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 36224; Mature: 36224

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNQLLDAKVASEEQETVLRPSLLNDFTGQNQMKSNLKIFITSSIERDESLDHTLLHGPP
CCCCCCCHHHCCCHHHHHHCHHHHHHCCCCHHHHCCEEEEEEECCCCCCCCCCCEECCCC
GLGKTTIAQIIAKEKNVNLKSTAGPILSKAADLAAILTNLQKNDVLFIDEIHRLNIHVEE
CCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECHHHHCEEHHH
ILYSAMEDFSLDIMIGEGPSARSVKIYLPKFTLIGATTRLGLISKPLCDRFGIHLKLNFY
HHHHHHHCCEEEEEECCCCCCCEEEEEECCEEEEECHHHHHHHCCCHHHHCCCEEEEEEE
SCEELTKIVERGAKVLNVALDTNAAIEIANRSRGTPRIALRLLKRVRDFGIYQNINPLNQ
CHHHHHHHHHCCHHEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHH
QITDYALNQLGIDKLGLDSSDHKYLRFIAENYDGGPVGIDTIAAALSEQRDTIEEMIEPY
HHHHHHHHHCCCHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LIQIGFVQRTQRGRVITANALKHLH
HHHHHHHHHCCCCCEEEHHHHHHCC
>Mature Secondary Structure
MKNQLLDAKVASEEQETVLRPSLLNDFTGQNQMKSNLKIFITSSIERDESLDHTLLHGPP
CCCCCCCHHHCCCHHHHHHCHHHHHHCCCCHHHHCCEEEEEEECCCCCCCCCCCEECCCC
GLGKTTIAQIIAKEKNVNLKSTAGPILSKAADLAAILTNLQKNDVLFIDEIHRLNIHVEE
CCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECHHHHCEEHHH
ILYSAMEDFSLDIMIGEGPSARSVKIYLPKFTLIGATTRLGLISKPLCDRFGIHLKLNFY
HHHHHHHCCEEEEEECCCCCCCEEEEEECCEEEEECHHHHHHHCCCHHHHCCCEEEEEEE
SCEELTKIVERGAKVLNVALDTNAAIEIANRSRGTPRIALRLLKRVRDFGIYQNINPLNQ
CHHHHHHHHHCCHHEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHH
QITDYALNQLGIDKLGLDSSDHKYLRFIAENYDGGPVGIDTIAAALSEQRDTIEEMIEPY
HHHHHHHHHCCCHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LIQIGFVQRTQRGRVITANALKHLH
HHHHHHHHHCCCCCEEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA