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The map label for this gene is tme [C]

Identifier: 189183489

GI number: 189183489

Start: 595505

End: 596806

Strand: Reverse

Name: tme [C]

Synonym: OTT_0582

Alternate gene names: 189183489

Gene position: 596806-595505 (Counterclockwise)

Preceding gene: 189183490

Following gene: 189183488

Centisome position: 29.71

GC content: 34.18

Gene sequence:

>1302_bases
ATGACTAATAACTTATCTAAAAATGATTCATTGATCTCGAAGTTAAGTCTTGAATATCATAAAAAAGATAACCTGCCAGG
CAAAATAGCTATGATAACTACCAAGCCACTTGCAACTCAACATGACTTAGCGCTAGCTTATTCTCCTGGTGTATCTGCTC
CATGTCTAAAGATCGTTGATAATATTAATGCAATTTATGACTATACCTCTCGAGGTAACACAGTTGCAGTAATTTCAAAT
GGTACAGCAGTTCTAGGGCTAGGTAATTTAGGAGCTGCTGCTGCTAAACCTGTTATGGAAGGTAAAGCGGCATTATTTAA
AAAATTTGCTGATATTGATGCTATAGATCTTGAGATAGATACTGAAGATCCTTATGAAATTATTAAGCTTATTCAATATC
TTAAATACAATTGGGGGGGTATTAATTTAGAAGATATTAAGGCTCCTGAATGCTTTATTATAGAAAATAAGCTACAAGAA
CTTGTAGATATTCCAGTATTTCATGATGACCAACATGGTACAGCTGTTGCAGTTACGGCAGGAGTTATTAATGCTGCTGA
TCTTACAGGACGTCAGAATTTAAGAAATTTAAAAGTTATCATTAATGGAGCTGGAGCTGCTGGAATTGCTTGTGCAGAAT
TATTAAAGTCTATAGGTATAGAAAATGTAATATTATGCGATAGCCAGGGCGTAATTTATAAAGGACGAACTAAAGGCATG
AATGTATGGAAAGAAGCTCATGCTATCGATACTAAGTATAGAACTCTTAAAGAAGCAATAATTGGGGCTGATTTATTTTT
AGGGTTATCAGTTAAAGGTGCTTTAACACAAGAAATGTTAGCTACTATGGCTGATAAGCCAATTATCTTTGCCTTAGCTA
ATCCAGATCCAGAAATTATGCCTGATGAAGTTAAGTTTGTACGTCCGAATGCTATTGTTGCTACAGGAAGATCTGATTTT
AGTAACCAAATAAATAATGTAATAGTGTTTCCTTATATTTTTAGAGGAGCATTAGATGTTATGGCAACAACAATAAATAA
CTCAATGAAGATAGCTGCAGCTAAGGAAATAGCAGCACTTGCTACTCATCCAGTACCAGATTTAGTTACTAAAGCTCATT
TAGGTCGAAAAATGGAATATGGATTTGATTACATTCTTCCTTCACCATTTGACCCAAGGCTACTTTATGCTATTCCACCA
TTAGTAGCTAGTACAGCAATGAAAACTGGAGTAGCTAGAAAAAATATTGATCTTCAAAAATATAAAAACCATCTTTTTGA
TAAAGCTGCATTTGCTGAGTAA

Upstream 100 bases:

>100_bases
GACTCCATGGCAACAATCATTACTTTTACTACAATATTTTCCTTATTGTCTTTATCAATTATTATGTATATCTTTGCTTA
AAATTAATAGGATAATCTTT

Downstream 100 bases:

>100_bases
ATAGTTATATAAGTAGCTATAATAACGTAGATAATAGTAAAGAAGATGGCAATTTTTTTGTACACATCTAAACTTTACAA
ATTCTTACGGCTATTTTATC

Product: malate oxidoreductase and phosphate acetyltransferase

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MTNNLSKNDSLISKLSLEYHKKDNLPGKIAMITTKPLATQHDLALAYSPGVSAPCLKIVDNINAIYDYTSRGNTVAVISN
GTAVLGLGNLGAAAAKPVMEGKAALFKKFADIDAIDLEIDTEDPYEIIKLIQYLKYNWGGINLEDIKAPECFIIENKLQE
LVDIPVFHDDQHGTAVAVTAGVINAADLTGRQNLRNLKVIINGAGAAGIACAELLKSIGIENVILCDSQGVIYKGRTKGM
NVWKEAHAIDTKYRTLKEAIIGADLFLGLSVKGALTQEMLATMADKPIIFALANPDPEIMPDEVKFVRPNAIVATGRSDF
SNQINNVIVFPYIFRGALDVMATTINNSMKIAAAKEIAALATHPVPDLVTKAHLGRKMEYGFDYILPSPFDPRLLYAIPP
LVASTAMKTGVARKNIDLQKYKNHLFDKAAFAE

Sequences:

>Translated_433_residues
MTNNLSKNDSLISKLSLEYHKKDNLPGKIAMITTKPLATQHDLALAYSPGVSAPCLKIVDNINAIYDYTSRGNTVAVISN
GTAVLGLGNLGAAAAKPVMEGKAALFKKFADIDAIDLEIDTEDPYEIIKLIQYLKYNWGGINLEDIKAPECFIIENKLQE
LVDIPVFHDDQHGTAVAVTAGVINAADLTGRQNLRNLKVIINGAGAAGIACAELLKSIGIENVILCDSQGVIYKGRTKGM
NVWKEAHAIDTKYRTLKEAIIGADLFLGLSVKGALTQEMLATMADKPIIFALANPDPEIMPDEVKFVRPNAIVATGRSDF
SNQINNVIVFPYIFRGALDVMATTINNSMKIAAAKEIAALATHPVPDLVTKAHLGRKMEYGFDYILPSPFDPRLLYAIPP
LVASTAMKTGVARKNIDLQKYKNHLFDKAAFAE
>Mature_432_residues
TNNLSKNDSLISKLSLEYHKKDNLPGKIAMITTKPLATQHDLALAYSPGVSAPCLKIVDNINAIYDYTSRGNTVAVISNG
TAVLGLGNLGAAAAKPVMEGKAALFKKFADIDAIDLEIDTEDPYEIIKLIQYLKYNWGGINLEDIKAPECFIIENKLQEL
VDIPVFHDDQHGTAVAVTAGVINAADLTGRQNLRNLKVIINGAGAAGIACAELLKSIGIENVILCDSQGVIYKGRTKGMN
VWKEAHAIDTKYRTLKEAIIGADLFLGLSVKGALTQEMLATMADKPIIFALANPDPEIMPDEVKFVRPNAIVATGRSDFS
NQINNVIVFPYIFRGALDVMATTINNSMKIAAAKEIAALATHPVPDLVTKAHLGRKMEYGFDYILPSPFDPRLLYAIPPL
VASTAMKTGVARKNIDLQKYKNHLFDKAAFAE

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=383, Percent_Identity=26.3707571801567, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI62420880, Length=383, Percent_Identity=26.3707571801567, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI239049447, Length=383, Percent_Identity=26.3707571801567, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI4505143, Length=345, Percent_Identity=26.9565217391304, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1788806, Length=438, Percent_Identity=52.7397260273973, Blast_Score=450, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6322823, Length=233, Percent_Identity=32.1888412017167, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI78707242, Length=412, Percent_Identity=25.9708737864078, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI78707238, Length=412, Percent_Identity=25.9708737864078, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24646388, Length=348, Percent_Identity=27.2988505747126, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI78707240, Length=350, Percent_Identity=26.8571428571429, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24646386, Length=348, Percent_Identity=27.2988505747126, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 46951; Mature: 46820

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNNLSKNDSLISKLSLEYHKKDNLPGKIAMITTKPLATQHDLALAYSPGVSAPCLKIVD
CCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEECCCCCCHHHHHHH
NINAIYDYTSRGNTVAVISNGTAVLGLGNLGAAAAKPVMEGKAALFKKFADIDAIDLEID
CCHHEEEECCCCCEEEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
TEDPYEIIKLIQYLKYNWGGINLEDIKAPECFIIENKLQELVDIPVFHDDQHGTAVAVTA
CCCHHHHHHHHHHHHCCCCCCCHHHCCCCCEEEEHHHHHHHHCCCCEECCCCCCEEEEEE
GVINAADLTGRQNLRNLKVIINGAGAAGIACAELLKSIGIENVILCDSQGVIYKGRTKGM
CCEECHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCC
NVWKEAHAIDTKYRTLKEAIIGADLFLGLSVKGALTQEMLATMADKPIIFALANPDPEIM
HHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCC
PDEVKFVRPNAIVATGRSDFSNQINNVIVFPYIFRGALDVMATTINNSMKIAAAKEIAAL
CCCCEEECCCEEEEECCHHHHHHHCCEEEEHHHHHHHHHHHHHHHCCCEEEHHHHHHHHH
ATHPVPDLVTKAHLGRKMEYGFDYILPSPFDPRLLYAIPPLVASTAMKTGVARKNIDLQK
HCCCCHHHHHHHHHCCCHHCCCCEECCCCCCCEEEEECCHHHHHHHHHHCCHHCCCCHHH
YKNHLFDKAAFAE
HHHHHHHHHHCCC
>Mature Secondary Structure 
TNNLSKNDSLISKLSLEYHKKDNLPGKIAMITTKPLATQHDLALAYSPGVSAPCLKIVD
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEECCCCCCHHHHHHH
NINAIYDYTSRGNTVAVISNGTAVLGLGNLGAAAAKPVMEGKAALFKKFADIDAIDLEID
CCHHEEEECCCCCEEEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
TEDPYEIIKLIQYLKYNWGGINLEDIKAPECFIIENKLQELVDIPVFHDDQHGTAVAVTA
CCCHHHHHHHHHHHHCCCCCCCHHHCCCCCEEEEHHHHHHHHCCCCEECCCCCCEEEEEE
GVINAADLTGRQNLRNLKVIINGAGAAGIACAELLKSIGIENVILCDSQGVIYKGRTKGM
CCEECHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCC
NVWKEAHAIDTKYRTLKEAIIGADLFLGLSVKGALTQEMLATMADKPIIFALANPDPEIM
HHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCC
PDEVKFVRPNAIVATGRSDFSNQINNVIVFPYIFRGALDVMATTINNSMKIAAAKEIAAL
CCCCEEECCCEEEEECCHHHHHHHCCEEEEHHHHHHHHHHHHHHHCCCEEEHHHHHHHHH
ATHPVPDLVTKAHLGRKMEYGFDYILPSPFDPRLLYAIPPLVASTAMKTGVARKNIDLQK
HCCCCHHHHHHHHHCCCHHCCCCEECCCCCCCEEEEECCHHHHHHHHHHCCHHCCCCHHH
YKNHLFDKAAFAE
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]