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The map label for this gene is rne [H]

Identifier: 189183199

GI number: 189183199

Start: 294486

End: 296513

Strand: Reverse

Name: rne [H]

Synonym: OTT_0292

Alternate gene names: 189183199

Gene position: 296513-294486 (Counterclockwise)

Preceding gene: 189183206

Following gene: 189183198

Centisome position: 14.76

GC content: 29.44

Gene sequence:

>2028_bases
ATGACTAAACGAATTCTAGTAGATGCAAGTTACCCTAATGAAACTAGAGTAGCTTTAATAGATAATAATTGCCGTATAGC
AGAAATTGAATATGAAACAGCAAACAAACATTTAATCAAGGGGAATATATATCTTGCAACAATTACTAAGATAGAACCTT
CTTTGCAAGCTGCATTTATTGATTATAGTAATGGAAAAAGCGGCTTTCTTCCATTTAGTGAAATACACCCAGATTATTAT
AATAATGTTACTAACAGCTTACCATTTGATACTATTCCTTTAGTAGAAATAACTTCAGAGGAGATTAAGGAGCAAGAATC
ATGCAAAAATATTCAAAATGAAGAAGACATCGATACAGAAGAATCTGATGCTGATGACAGTAAAATAATTACAGCATTTA
AAAATACTACAAATATCCAAAACGATGAAATTGAACCAGTAAGCAAACAAAACAGCCAAGAAATTGCAGAATCTTTGCCA
CCTCATAAGCAACAAGAAATTCAAGATGTAATTAGCAGCGGGCAGATTGTTCTAGTTCAAGCAACTAAGGAATCTAGAGG
TAATAAAAATGCATCCTTTACCACTTATATTTCTTTATCAGGTAAATATTGTATACTAACCCCTAACTTATCAAACCATA
GTGGCATTTCAAGAAGAATTACAAATTCAGAAGATAGAAAACAATTAAAAACAATCGTAGGCAATCTAATATCAAAAATA
ACAAAGACATCAAGCGTTATTATTAGAACTGCTGGCTCTCGTAGAACTGCATATGAAATTAAAAAAGATTTTAATTATTT
ATTAAAGCTCTGGAATGAAATTATCAATACAGCTAAGCAAGCTAAGCCACCTGCATTCATTTATGTTGAAAATGATATTA
TCAGAAAAACTATTCTTGATATTTATAATATTTCTGTGCAAGAAATGATTATACAAGGTAAAGCAGCTTATGATTATGCT
GTTAAGTTTATGAATGCTATTTTGCCATCAGAAGTACATAAAATTAAAGAACATAAATCTACAATTCCAATCTTTGCTCA
ATATAATCTAGAAGAGCAGCTTGCAACATTATATAAACCAATAGTTACTCTTCCATCTGGAGGGTATATTGTTATTAATC
CAACAGAAGCTTTAATCGCAATTGATGTAAACTCTGGCAAAGATACTTCTGAACAAAATATCGAAGAAACAGCTGTCAAA
ACTAATTTAGAGGCAGCAAAAGAAATTAGTCACCAAATTAAGTTAAGAAACTTATCAGGACTAATTACTATAGACTTTAT
TTGCATGACAGATTCTAAGAATCATAAATTAATTGAAAAAAGCTTCAAAAAATATCTTGCTAAAGATAAAGCAAAAATAC
AAGTTGATTCTATTAACTCTTTTGGAGTATTACAACTGTCTAGGCAAAGATTAAGACCATCTTTCTTAGAATCTAACTCA
GTTATATGCTCACATTGCAGTGGTAAAGGAATAGTTAGAGCTGAAGAATCTAATGCTATGATTATATTACGTACTATCGA
AAATGAAATACATACATCTAAATCAAATATTATAAATGTTTATGCTCACTTACATTCAGTTACATATATTCTAAATAATA
AGCGAGCTGAAATAGCTGATATAGAAAAAAAGTATAATGTACAGTTGAAATTTTACAATGATTTTCAATCTACTTCAGAT
AGTTTTTCTATAGAAAAAGTTACATTATCTGCAAATACTACTAAAAGCGCTCTTACAGAACAGCCAGTAGTAACTAGTGC
TAAATTATTGGAGAATATAGAAAACCCTGGTTCACAAGCAATATCTAATACTAATATTTCAGTCTCTAAAATATTATCAA
TAAAATCAAATAAAAGGAGACCAAGGAAACCAACTCTTAAACTAGATTTACCAGCAGAACAATCAGTTAAACAAATATCT
AAACCAGTAAATGATAAAAAAGATAATGCTCCAAATAATAAAAACCAACCTACTAGGCCAATAAAAAGAAGAACAAAACC
AAGTATACTAGAGACTACAAAATCATAA

Upstream 100 bases:

>100_bases
CATACTTACAACAAGTATGCAATAATAACCTGCATGACTTCAAATTGTTAAATTTGTAAATTATAGAACAGTCAGTAGAG
TTTATGGAGAATTTATAAAG

Downstream 100 bases:

>100_bases
TTGTGATGTCCTACTATAGCAAATAACAGTAGCTAGCTATATATGGTAGGTGAGTCAAGATAAGGAGCATTATAATCTTA
CTGGTAAAAGTCCAGTTATG

Product: ribonuclease E

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 675; Mature: 674

Protein sequence:

>675_residues
MTKRILVDASYPNETRVALIDNNCRIAEIEYETANKHLIKGNIYLATITKIEPSLQAAFIDYSNGKSGFLPFSEIHPDYY
NNVTNSLPFDTIPLVEITSEEIKEQESCKNIQNEEDIDTEESDADDSKIITAFKNTTNIQNDEIEPVSKQNSQEIAESLP
PHKQQEIQDVISSGQIVLVQATKESRGNKNASFTTYISLSGKYCILTPNLSNHSGISRRITNSEDRKQLKTIVGNLISKI
TKTSSVIIRTAGSRRTAYEIKKDFNYLLKLWNEIINTAKQAKPPAFIYVENDIIRKTILDIYNISVQEMIIQGKAAYDYA
VKFMNAILPSEVHKIKEHKSTIPIFAQYNLEEQLATLYKPIVTLPSGGYIVINPTEALIAIDVNSGKDTSEQNIEETAVK
TNLEAAKEISHQIKLRNLSGLITIDFICMTDSKNHKLIEKSFKKYLAKDKAKIQVDSINSFGVLQLSRQRLRPSFLESNS
VICSHCSGKGIVRAEESNAMIILRTIENEIHTSKSNIINVYAHLHSVTYILNNKRAEIADIEKKYNVQLKFYNDFQSTSD
SFSIEKVTLSANTTKSALTEQPVVTSAKLLENIENPGSQAISNTNISVSKILSIKSNKRRPRKPTLKLDLPAEQSVKQIS
KPVNDKKDNAPNNKNQPTRPIKRRTKPSILETTKS

Sequences:

>Translated_675_residues
MTKRILVDASYPNETRVALIDNNCRIAEIEYETANKHLIKGNIYLATITKIEPSLQAAFIDYSNGKSGFLPFSEIHPDYY
NNVTNSLPFDTIPLVEITSEEIKEQESCKNIQNEEDIDTEESDADDSKIITAFKNTTNIQNDEIEPVSKQNSQEIAESLP
PHKQQEIQDVISSGQIVLVQATKESRGNKNASFTTYISLSGKYCILTPNLSNHSGISRRITNSEDRKQLKTIVGNLISKI
TKTSSVIIRTAGSRRTAYEIKKDFNYLLKLWNEIINTAKQAKPPAFIYVENDIIRKTILDIYNISVQEMIIQGKAAYDYA
VKFMNAILPSEVHKIKEHKSTIPIFAQYNLEEQLATLYKPIVTLPSGGYIVINPTEALIAIDVNSGKDTSEQNIEETAVK
TNLEAAKEISHQIKLRNLSGLITIDFICMTDSKNHKLIEKSFKKYLAKDKAKIQVDSINSFGVLQLSRQRLRPSFLESNS
VICSHCSGKGIVRAEESNAMIILRTIENEIHTSKSNIINVYAHLHSVTYILNNKRAEIADIEKKYNVQLKFYNDFQSTSD
SFSIEKVTLSANTTKSALTEQPVVTSAKLLENIENPGSQAISNTNISVSKILSIKSNKRRPRKPTLKLDLPAEQSVKQIS
KPVNDKKDNAPNNKNQPTRPIKRRTKPSILETTKS
>Mature_674_residues
TKRILVDASYPNETRVALIDNNCRIAEIEYETANKHLIKGNIYLATITKIEPSLQAAFIDYSNGKSGFLPFSEIHPDYYN
NVTNSLPFDTIPLVEITSEEIKEQESCKNIQNEEDIDTEESDADDSKIITAFKNTTNIQNDEIEPVSKQNSQEIAESLPP
HKQQEIQDVISSGQIVLVQATKESRGNKNASFTTYISLSGKYCILTPNLSNHSGISRRITNSEDRKQLKTIVGNLISKIT
KTSSVIIRTAGSRRTAYEIKKDFNYLLKLWNEIINTAKQAKPPAFIYVENDIIRKTILDIYNISVQEMIIQGKAAYDYAV
KFMNAILPSEVHKIKEHKSTIPIFAQYNLEEQLATLYKPIVTLPSGGYIVINPTEALIAIDVNSGKDTSEQNIEETAVKT
NLEAAKEISHQIKLRNLSGLITIDFICMTDSKNHKLIEKSFKKYLAKDKAKIQVDSINSFGVLQLSRQRLRPSFLESNSV
ICSHCSGKGIVRAEESNAMIILRTIENEIHTSKSNIINVYAHLHSVTYILNNKRAEIADIEKKYNVQLKFYNDFQSTSDS
FSIEKVTLSANTTKSALTEQPVVTSAKLLENIENPGSQAISNTNISVSKILSIKSNKRRPRKPTLKLDLPAEQSVKQISK
PVNDKKDNAPNNKNQPTRPIKRRTKPSILETTKS

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. It also cleaves RNA I, a molecule that controls the replication of colE1 plasmid DNA. It is the major endoribonuclease participating in mRNA turnover in E.coli. It initiates the decay of RNAs by

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=386, Percent_Identity=39.6373056994819, Blast_Score=291, Evalue=7e-80,
Organism=Escherichia coli, GI87082235, Length=397, Percent_Identity=28.2115869017632, Blast_Score=179, Evalue=5e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 75885; Mature: 75754

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRILVDASYPNETRVALIDNNCRIAEIEYETANKHLIKGNIYLATITKIEPSLQAAFI
CCCEEEEECCCCCCCEEEEEECCCEEEEEEECCCCCEEEECCEEEEEEEEECCCEEEEEE
DYSNGKSGFLPFSEIHPDYYNNVTNSLPFDTIPLVEITSEEIKEQESCKNIQNEEDIDTE
EECCCCCCCCCHHHCCCHHHHCCCCCCCCCCCCEEEECHHHHHHHHHHHCCCCCCCCCCC
ESDADDSKIITAFKNTTNIQNDEIEPVSKQNSQEIAESLPPHKQQEIQDVISSGQIVLVQ
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCEEEEE
ATKESRGNKNASFTTYISLSGKYCILTPNLSNHSGISRRITNSEDRKQLKTIVGNLISKI
ECCCCCCCCCCEEEEEEEECCCEEEECCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHH
TKTSSVIIRTAGSRRTAYEIKKDFNYLLKLWNEIINTAKQAKPPAFIYVENDIIRKTILD
HCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHH
IYNISVQEMIIQGKAAYDYAVKFMNAILPSEVHKIKEHKSTIPIFAQYNLEEQLATLYKP
HHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHH
IVTLPSGGYIVINPTEALIAIDVNSGKDTSEQNIEETAVKTNLEAAKEISHQIKLRNLSG
HEECCCCCEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCC
LITIDFICMTDSKNHKLIEKSFKKYLAKDKAKIQVDSINSFGVLQLSRQRLRPSFLESNS
EEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCHHHHCCCC
VICSHCSGKGIVRAEESNAMIILRTIENEIHTSKSNIINVYAHLHSVTYILNNKRAEIAD
EEEEECCCCCEEEEECCCCEEEEEECCHHHHCCHHHHHHHHHHHHHHEEEECCCCCHHHH
IEKKYNVQLKFYNDFQSTSDSFSIEKVTLSANTTKSALTEQPVVTSAKLLENIENPGSQA
CCHHEEEEEEEECCCCCCCCCCEEEEEEEECCCCHHHHCCCCCHHHHHHHHHHCCCCHHH
ISNTNISVSKILSIKSNKRRPRKPTLKLDLPAEQSVKQISKPVNDKKDNAPNNKNQPTRP
HCCCCCCHHHHEEECCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCCCH
IKRRTKPSILETTKS
HHHCCCCCHHHCCCC
>Mature Secondary Structure 
TKRILVDASYPNETRVALIDNNCRIAEIEYETANKHLIKGNIYLATITKIEPSLQAAFI
CCEEEEECCCCCCCEEEEEECCCEEEEEEECCCCCEEEECCEEEEEEEEECCCEEEEEE
DYSNGKSGFLPFSEIHPDYYNNVTNSLPFDTIPLVEITSEEIKEQESCKNIQNEEDIDTE
EECCCCCCCCCHHHCCCHHHHCCCCCCCCCCCCEEEECHHHHHHHHHHHCCCCCCCCCCC
ESDADDSKIITAFKNTTNIQNDEIEPVSKQNSQEIAESLPPHKQQEIQDVISSGQIVLVQ
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCEEEEE
ATKESRGNKNASFTTYISLSGKYCILTPNLSNHSGISRRITNSEDRKQLKTIVGNLISKI
ECCCCCCCCCCEEEEEEEECCCEEEECCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHH
TKTSSVIIRTAGSRRTAYEIKKDFNYLLKLWNEIINTAKQAKPPAFIYVENDIIRKTILD
HCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHH
IYNISVQEMIIQGKAAYDYAVKFMNAILPSEVHKIKEHKSTIPIFAQYNLEEQLATLYKP
HHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHH
IVTLPSGGYIVINPTEALIAIDVNSGKDTSEQNIEETAVKTNLEAAKEISHQIKLRNLSG
HEECCCCCEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCC
LITIDFICMTDSKNHKLIEKSFKKYLAKDKAKIQVDSINSFGVLQLSRQRLRPSFLESNS
EEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCHHHHCCCC
VICSHCSGKGIVRAEESNAMIILRTIENEIHTSKSNIINVYAHLHSVTYILNNKRAEIAD
EEEEECCCCCEEEEECCCCEEEEEECCHHHHCCHHHHHHHHHHHHHHEEEECCCCCHHHH
IEKKYNVQLKFYNDFQSTSDSFSIEKVTLSANTTKSALTEQPVVTSAKLLENIENPGSQA
CCHHEEEEEEEECCCCCCCCCCEEEEEEEECCCCHHHHCCCCCHHHHHHHHHHCCCCHHH
ISNTNISVSKILSIKSNKRRPRKPTLKLDLPAEQSVKQISKPVNDKKDNAPNNKNQPTRP
HCCCCCCHHHHEEECCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCCCH
IKRRTKPSILETTKS
HHHCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 1447789; 1704367; 2011493; 8415644 [H]