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The map label for this gene is dnaB [H]

Identifier: 189183177

GI number: 189183177

Start: 269215

End: 270600

Strand: Direct

Name: dnaB [H]

Synonym: OTT_0270

Alternate gene names: 189183177

Gene position: 269215-270600 (Clockwise)

Preceding gene: 189183176

Following gene: 189183180

Centisome position: 13.4

GC content: 31.53

Gene sequence:

>1386_bases
ATGGAAAAAGATCTTGCAAAGATTGCTCCAAGTAATATTCAGGCAGAGCAAATGATACTTGGAGCAATTCTGATTAACAA
TCGTGCGCTATATAACATTAACGAATTTTTACTGCCAGAACATTTTTATGAACCATTACATGGCAAAATATACAAGTCAA
TTAATCTCATTATTAGTAAAGGAATTAGTGCTAGTGTAATTTCGCTCAAAAATATGCTAGGCAATGAACTCGCATTTGAG
GAAATAGGTGGAGTGGATTATCTAGCTAAACTTACAACTTTAGCATTAAGTATAGTTAATGTTAATGAGTACGGCAAAAT
AGTATATGATCTTGCGCTGCGGCGTTATTTTATTGAAATTGGAGAAAAAATAGTAACAAATGCATATTCTTCTACTTTAG
CAGATTTAGCTATAACTCAGATCGAAACTGCTGAATCTCAATTATATGATCTAGGTTCAAGAGGAACTTTAAGTAAAGGA
TTTACAAAATTACAAACTTCAATTGAAGAATCATGGACATCAATTTCATCTGCTATTAAAAATAAAAACTCTATTAACGG
TATTAGTAGTGGACTACTTGACCTTGATTCAAAGCTTGGAGGATTTAAAAATTCTGACCTAATAATATTAGCTGGCAGAC
CTTCAATGGGTAAAACTGCTTTAGGAGTTAACTTAGCAATAAATGCTTGTAAATATTTTTTATCATCATCTACTCAACAA
AATAGCAAAGTTTCTAATATAACACCATCAGTTGGATTCTTTTCTTTAGAAATGTCATCTCAGCAAATCTCAACTCGAAT
TCTTTCTATAGAATCAGAAATTAACAGCTCTGCATTATTTAACGGTAAAATAGATGAACAAGATGTTGATAAGTTGAAGA
CTGTACAAAATGAAATACAAAAGTGGAATTTTTTTATAGATGATGCTCCAGCAATCTCGATATCTGCAATTAGATCTCGA
GCTCGTAGACTTAAACGTACTCATAATTTAGCAATATTATTTGTTGATTATTTACAGCTAATAAAAATTGATAACAGAAG
AAGTCAGTATAATAGAGTGCAGGAGATTTCTGAAATTACTCAGAGCTTAAAAGCTCTTGCTAAAGAGCTCAATATTTCAA
TCATTGCGTTATCTCAATTATCTAGAGCTGTAGAACAAAGGTCAGATAAAAAGCCTCTACTATCAGATCTACGAGAATCA
GGCTCCATTGAACAAGATGCCGATATTGTAATGCTTATATATCGTGACGAATATTACTTGTCTAGATCAGAACCAGATCT
TTTAGATATTTTTATATATCTATATGTCCCTGAAATTTCTTTCAGGTTGGGACGAAAATTTTTTTCTGGTAAGGCTATAT
TTTCTTCTTTTCCTTTTTCACTATAG

Upstream 100 bases:

>100_bases
GAGAGAACTATCTGAAAGATCTTGAGCCCGCTTTTAAAGCTCAAGGATTTTCTTTCGAGTTAGTTAAGTTTAGTAATTTT
AATAAAATTTAAAGGGTGAT

Downstream 100 bases:

>100_bases
TGCTGAAAATTTTTCAGAATTTTTATGCAAGGGACATTACGTAACTTAACATTGTCTTTAATTATTGTCCTATTTTCAAC
TTCAATAAAACCTGCATCTC

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISASVISLKNMLGNELAFE
EIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYFIEIGEKIVTNAYSSTLADLAITQIETAESQLYDLGSRGTLSKG
FTKLQTSIEESWTSISSAIKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLSSSTQQ
NSKVSNITPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIDEQDVDKLKTVQNEIQKWNFFIDDAPAISISAIRSR
ARRLKRTHNLAILFVDYLQLIKIDNRRSQYNRVQEISEITQSLKALAKELNISIIALSQLSRAVEQRSDKKPLLSDLRES
GSIEQDADIVMLIYRDEYYLSRSEPDLLDIFIYLYVPEISFRLGRKFFSGKAIFSSFPFSL

Sequences:

>Translated_461_residues
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISASVISLKNMLGNELAFE
EIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYFIEIGEKIVTNAYSSTLADLAITQIETAESQLYDLGSRGTLSKG
FTKLQTSIEESWTSISSAIKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLSSSTQQ
NSKVSNITPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIDEQDVDKLKTVQNEIQKWNFFIDDAPAISISAIRSR
ARRLKRTHNLAILFVDYLQLIKIDNRRSQYNRVQEISEITQSLKALAKELNISIIALSQLSRAVEQRSDKKPLLSDLRES
GSIEQDADIVMLIYRDEYYLSRSEPDLLDIFIYLYVPEISFRLGRKFFSGKAIFSSFPFSL
>Mature_461_residues
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISASVISLKNMLGNELAFE
EIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYFIEIGEKIVTNAYSSTLADLAITQIETAESQLYDLGSRGTLSKG
FTKLQTSIEESWTSISSAIKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLSSSTQQ
NSKVSNITPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIDEQDVDKLKTVQNEIQKWNFFIDDAPAISISAIRSR
ARRLKRTHNLAILFVDYLQLIKIDNRRSQYNRVQEISEITQSLKALAKELNISIIALSQLSRAVEQRSDKKPLLSDLRES
GSIEQDADIVMLIYRDEYYLSRSEPDLLDIFIYLYVPEISFRLGRKFFSGKAIFSSFPFSL

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=425, Percent_Identity=35.5294117647059, Blast_Score=255, Evalue=5e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 51530; Mature: 51530

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISK
CCCCHHHCCCCCCCHHHHHHHHHHCCCCEEECCHHHHCCHHHHCCHHHHHHHHHHHHHHC
GISASVISLKNMLGNELAFEEIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYFIEI
CCCHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHH
GEKIVTNAYSSTLADLAITQIETAESQLYDLGSRGTLSKGFTKLQTSIEESWTSISSAIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLSSSTQQ
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCC
NSKVSNITPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIDEQDVDKLKTVQNEIQ
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHH
KWNFFIDDAPAISISAIRSRARRLKRTHNLAILFVDYLQLIKIDNRRSQYNRVQEISEIT
HCCEEECCCCCHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHH
QSLKALAKELNISIIALSQLSRAVEQRSDKKPLLSDLRESGSIEQDADIVMLIYRDEYYL
HHHHHHHHHHCEEEEEHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCEEE
SRSEPDLLDIFIYLYVPEISFRLGRKFFSGKAIFSSFPFSL
CCCCCCHHHEEEHHHCCHHHHHHHHHHHCCCHHHCCCCCCC
>Mature Secondary Structure
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISK
CCCCHHHCCCCCCCHHHHHHHHHHCCCCEEECCHHHHCCHHHHCCHHHHHHHHHHHHHHC
GISASVISLKNMLGNELAFEEIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYFIEI
CCCHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHH
GEKIVTNAYSSTLADLAITQIETAESQLYDLGSRGTLSKGFTKLQTSIEESWTSISSAIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLSSSTQQ
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCC
NSKVSNITPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIDEQDVDKLKTVQNEIQ
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHH
KWNFFIDDAPAISISAIRSRARRLKRTHNLAILFVDYLQLIKIDNRRSQYNRVQEISEIT
HCCEEECCCCCHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHH
QSLKALAKELNISIIALSQLSRAVEQRSDKKPLLSDLRESGSIEQDADIVMLIYRDEYYL
HHHHHHHHHHCEEEEEHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCEEE
SRSEPDLLDIFIYLYVPEISFRLGRKFFSGKAIFSSFPFSL
CCCCCCHHHEEEHHHCCHHHHHHHHHHHCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA