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The map label for this gene is groEL

Identifier: 189183166

GI number: 189183166

Start: 255061

End: 256728

Strand: Direct

Name: groEL

Synonym: OTT_0259

Alternate gene names: 189183166

Gene position: 255061-256728 (Clockwise)

Preceding gene: 189183165

Following gene: 189183170

Centisome position: 12.7

GC content: 36.39

Gene sequence:

>1668_bases
ATGAGTAAACAAATTGTACATGGCGATCAATGTCGTAAAAAAATAATAGAAGGCATAAATGTTGTAGCTAATGCAGTAGG
TATAACATTAGGCCCCAAAGGTAGATGTGTAGCAATAGAACAATCTTATGGCCCACCTAAAATTACTAAAGATGGAGTTA
GTGTTGCTAAAGCAATTCAACTTAAGGATAAATCTCTTAATGTCGGAGCGCAATTTGTTATATCAGTTGCTAGTAAAACA
GCTGATGTAGCTGGTGATGGTACAACTACTGCAACAGTTATAGCTGATGCTGCAGTTAGAGAGTTAAATAAAGCTGAGGT
CGCAGGCATTGATATACAGGAAGTAAGAAAAGGCGCAGAAAAAGCTGTTGAAGCAGTTATTGCAGATGTACGTAAAAACA
GCAGCCCTGTTAAAAATGAGGAAGAAATTGCTCAAGTAGCTACTGTTTCATCTAATGGAGACCGCGAAATTGGTGAAAAG
ATTGCTAATGCAATGAAACAAGTTGGCCAAGAAGGAGTGATAACTGTTGAAGATTCTAAAAATTTTAACTTTGAAGTTGA
AGTTGTTAAAGGAATGAGATTTGACCGCGGATATATATCACAGTACTTTGCAACGAATCGTGAAAAGATGATTACCGAAT
TTGAAAATCCTTATATTTTGCTATTGGATCAGAAGGTATCTACAGTGCAGCCACTGGTTCCTGTGCTTGAAGCTGTTGCT
CACACTGGCAAGCCATTAGTATTGATTGCTGATGATGTAGACGGAGAAGCTCTTACTGCATTGATATTAAACAATTTAAA
AGGTTCTATTAAAGTAGTAGCTGTGAAGGCACCTGGTTTTGGTGATCGAAAAAAAGAAATGTTAGAAGATATTGCTATTT
TAACGAACGGAGAAGTAATTACTGAACAACTAGGAATTAAACTAGAAAAGGTTAATGATACATCTAAGTTAGGAACTGCA
AATAGAGTTATTGTTACAAAAGATCATACTACTATAGTTCATGATAAGAATAATAGTGATATAGAGAAGAAAGTTAATTC
TCGTTGTGAACAGATCCGTGAAGCAATTAAAGATACAACTTCAGATTATGAAAAAGAAAAATTACAAGAGCGCTTAGCAA
AACTTAGAAACGGTGTAGCTGTACTAAAAGTTGGTGGTGCTACTGAAGTAGAACAAAAAGAACGTAAGGATAGAGTAGAA
GATGCTTTACATGCTACTAGAGCAGCTGTTGAAGAAGGTATAGTGCCTGGTGGTGGTGTTGCGCTATTTTATGCTTCTCG
TGTTTTAGATAGTTTGAAGTTTGATAATGAAGATCAAAGGGTAGGTATTAACATTATCAAAAAAGTATTAGAAGCTCCGG
TAAGACAAATAGTTAAAAATGCTGGAGGCAAAGAGGATGTTGTTGTTAATGAACTAAGCAAGAGTAACGATAAAAACCGT
GGATTTGATGCTAGAACAATGCAATATGTTGATATGATAAAAGCAGGTATTGTGGATCCAACTAAAGTTGTACGTACTGC
ATTACAAGATGCTTTTTCTGTGGCTTCATTAGTAATTGCTACCTCAGCTATGATTACTGATCATGAGGAAGATAATAACA
CTGGTAACCGCAGTGGTGGTGGAGTAGGTGGCGGACATCATGGTGGTATGGGCGGAATGGATTTCTAA

Upstream 100 bases:

>100_bases
ATCAGATATATTATTAGTTAAAAGCTAAAATGCTTAATGCATTAATAATTAAAGGATGCTAGACTAATCAAATTATAAAA
TATTTTAAGGAGTTTTCAGT

Downstream 100 bases:

>100_bases
TCCGCCCATGTTTACCTTCAAAAAAAAAATAGAGTATAACTTTTGTATAAGGTTGCTAGTTATGTATTTTTTTACAAAAT
TGCAGTATAATATTTCAAGC

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60

Number of amino acids: Translated: 555; Mature: 554

Protein sequence:

>555_residues
MSKQIVHGDQCRKKIIEGINVVANAVGITLGPKGRCVAIEQSYGPPKITKDGVSVAKAIQLKDKSLNVGAQFVISVASKT
ADVAGDGTTTATVIADAAVRELNKAEVAGIDIQEVRKGAEKAVEAVIADVRKNSSPVKNEEEIAQVATVSSNGDREIGEK
IANAMKQVGQEGVITVEDSKNFNFEVEVVKGMRFDRGYISQYFATNREKMITEFENPYILLLDQKVSTVQPLVPVLEAVA
HTGKPLVLIADDVDGEALTALILNNLKGSIKVVAVKAPGFGDRKKEMLEDIAILTNGEVITEQLGIKLEKVNDTSKLGTA
NRVIVTKDHTTIVHDKNNSDIEKKVNSRCEQIREAIKDTTSDYEKEKLQERLAKLRNGVAVLKVGGATEVEQKERKDRVE
DALHATRAAVEEGIVPGGGVALFYASRVLDSLKFDNEDQRVGINIIKKVLEAPVRQIVKNAGGKEDVVVNELSKSNDKNR
GFDARTMQYVDMIKAGIVDPTKVVRTALQDAFSVASLVIATSAMITDHEEDNNTGNRSGGGVGGGHHGGMGGMDF

Sequences:

>Translated_555_residues
MSKQIVHGDQCRKKIIEGINVVANAVGITLGPKGRCVAIEQSYGPPKITKDGVSVAKAIQLKDKSLNVGAQFVISVASKT
ADVAGDGTTTATVIADAAVRELNKAEVAGIDIQEVRKGAEKAVEAVIADVRKNSSPVKNEEEIAQVATVSSNGDREIGEK
IANAMKQVGQEGVITVEDSKNFNFEVEVVKGMRFDRGYISQYFATNREKMITEFENPYILLLDQKVSTVQPLVPVLEAVA
HTGKPLVLIADDVDGEALTALILNNLKGSIKVVAVKAPGFGDRKKEMLEDIAILTNGEVITEQLGIKLEKVNDTSKLGTA
NRVIVTKDHTTIVHDKNNSDIEKKVNSRCEQIREAIKDTTSDYEKEKLQERLAKLRNGVAVLKVGGATEVEQKERKDRVE
DALHATRAAVEEGIVPGGGVALFYASRVLDSLKFDNEDQRVGINIIKKVLEAPVRQIVKNAGGKEDVVVNELSKSNDKNR
GFDARTMQYVDMIKAGIVDPTKVVRTALQDAFSVASLVIATSAMITDHEEDNNTGNRSGGGVGGGHHGGMGGMDF
>Mature_554_residues
SKQIVHGDQCRKKIIEGINVVANAVGITLGPKGRCVAIEQSYGPPKITKDGVSVAKAIQLKDKSLNVGAQFVISVASKTA
DVAGDGTTTATVIADAAVRELNKAEVAGIDIQEVRKGAEKAVEAVIADVRKNSSPVKNEEEIAQVATVSSNGDREIGEKI
ANAMKQVGQEGVITVEDSKNFNFEVEVVKGMRFDRGYISQYFATNREKMITEFENPYILLLDQKVSTVQPLVPVLEAVAH
TGKPLVLIADDVDGEALTALILNNLKGSIKVVAVKAPGFGDRKKEMLEDIAILTNGEVITEQLGIKLEKVNDTSKLGTAN
RVIVTKDHTTIVHDKNNSDIEKKVNSRCEQIREAIKDTTSDYEKEKLQERLAKLRNGVAVLKVGGATEVEQKERKDRVED
ALHATRAAVEEGIVPGGGVALFYASRVLDSLKFDNEDQRVGINIIKKVLEAPVRQIVKNAGGKEDVVVNELSKSNDKNRG
FDARTMQYVDMIKAGIVDPTKVVRTALQDAFSVASLVIATSAMITDHEEDNNTGNRSGGGVGGGHHGGMGGMDF

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family

Homologues:

Organism=Homo sapiens, GI41399285, Length=527, Percent_Identity=51.4231499051233, Blast_Score=500, Evalue=1e-141,
Organism=Homo sapiens, GI31542947, Length=527, Percent_Identity=51.4231499051233, Blast_Score=500, Evalue=1e-141,
Organism=Escherichia coli, GI1790586, Length=526, Percent_Identity=53.6121673003802, Blast_Score=540, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI17555558, Length=528, Percent_Identity=50.9469696969697, Blast_Score=501, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI193210679, Length=209, Percent_Identity=53.5885167464115, Blast_Score=214, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6323288, Length=528, Percent_Identity=50.5681818181818, Blast_Score=504, Evalue=1e-143,
Organism=Drosophila melanogaster, GI24641193, Length=527, Percent_Identity=49.7153700189753, Blast_Score=483, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24641191, Length=527, Percent_Identity=49.7153700189753, Blast_Score=483, Evalue=1e-136,
Organism=Drosophila melanogaster, GI45550936, Length=528, Percent_Identity=48.6742424242424, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI45550132, Length=528, Percent_Identity=48.6742424242424, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI45550935, Length=528, Percent_Identity=48.6742424242424, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI17864606, Length=556, Percent_Identity=39.2086330935252, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24584129, Length=530, Percent_Identity=36.0377358490566, Blast_Score=299, Evalue=3e-81,
Organism=Drosophila melanogaster, GI19921262, Length=530, Percent_Identity=36.0377358490566, Blast_Score=299, Evalue=3e-81,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): CH60_ORITI (B3CQ26)

Other databases:

- EMBL:   AP008981
- RefSeq:   YP_001936951.1
- GeneID:   6337843
- GenomeReviews:   AP008981_GR
- KEGG:   ott:OTT_0259
- HOGENOM:   HBG625289
- OMA:   NSDTSIG
- ProtClustDB:   PRK00013
- GO:   GO:0005737
- HAMAP:   MF_00600
- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423
- PANTHER:   PTHR11353
- PRINTS:   PR00298
- TIGRFAMs:   TIGR02348

Pfam domain/function: PF00118 Cpn60_TCP1; SSF48592 GroEL-ATPase

EC number: NA

Molecular weight: Translated: 59743; Mature: 59612

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQIVHGDQCRKKIIEGINVVANAVGITLGPKGRCVAIEQSYGPPKITKDGVSVAKAIQ
CCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHH
LKDKSLNVGAQFVISVASKTADVAGDGTTTATVIADAAVRELNKAEVAGIDIQEVRKGAE
HCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHH
KAVEAVIADVRKNSSPVKNEEEIAQVATVSSNGDREIGEKIANAMKQVGQEGVITVEDSK
HHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCC
NFNFEVEVVKGMRFDRGYISQYFATNREKMITEFENPYILLLDQKVSTVQPLVPVLEAVA
CCCEEEEEECCCCCCHHHHHHHHHCCHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHH
HTGKPLVLIADDVDGEALTALILNNLKGSIKVVAVKAPGFGDRKKEMLEDIAILTNGEVI
HCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCHHH
TEQLGIKLEKVNDTSKLGTANRVIVTKDHTTIVHDKNNSDIEKKVNSRCEQIREAIKDTT
HHHHCCEEEECCCCHHCCCCCEEEEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
SDYEKEKLQERLAKLRNGVAVLKVGGATEVEQKERKDRVEDALHATRAAVEEGIVPGGGV
CHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
ALFYASRVLDSLKFDNEDQRVGINIIKKVLEAPVRQIVKNAGGKEDVVVNELSKSNDKNR
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCC
GFDARTMQYVDMIKAGIVDPTKVVRTALQDAFSVASLVIATSAMITDHEEDNNTGNRSGG
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GVGGGHHGGMGGMDF
CCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKQIVHGDQCRKKIIEGINVVANAVGITLGPKGRCVAIEQSYGPPKITKDGVSVAKAIQ
CCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHH
LKDKSLNVGAQFVISVASKTADVAGDGTTTATVIADAAVRELNKAEVAGIDIQEVRKGAE
HCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHH
KAVEAVIADVRKNSSPVKNEEEIAQVATVSSNGDREIGEKIANAMKQVGQEGVITVEDSK
HHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCC
NFNFEVEVVKGMRFDRGYISQYFATNREKMITEFENPYILLLDQKVSTVQPLVPVLEAVA
CCCEEEEEECCCCCCHHHHHHHHHCCHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHH
HTGKPLVLIADDVDGEALTALILNNLKGSIKVVAVKAPGFGDRKKEMLEDIAILTNGEVI
HCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCHHH
TEQLGIKLEKVNDTSKLGTANRVIVTKDHTTIVHDKNNSDIEKKVNSRCEQIREAIKDTT
HHHHCCEEEECCCCHHCCCCCEEEEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
SDYEKEKLQERLAKLRNGVAVLKVGGATEVEQKERKDRVEDALHATRAAVEEGIVPGGGV
CHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
ALFYASRVLDSLKFDNEDQRVGINIIKKVLEAPVRQIVKNAGGKEDVVVNELSKSNDKNR
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCC
GFDARTMQYVDMIKAGIVDPTKVVRTALQDAFSVASLVIATSAMITDHEEDNNTGNRSGG
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GVGGGHHGGMGGMDF
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA