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The map label for this gene is dnaB [H]

Identifier: 189183159

GI number: 189183159

Start: 243525

End: 244694

Strand: Reverse

Name: dnaB [H]

Synonym: OTT_0252

Alternate gene names: 189183159

Gene position: 244694-243525 (Counterclockwise)

Preceding gene: 189183160

Following gene: 189183158

Centisome position: 12.18

GC content: 31.11

Gene sequence:

>1170_bases
ATGGAAAAAGATCTTGCAAAGATTGCTCCAAGTAATATTCAGGCAGAGCAAATGATACTTGGAGCAATTCTGATTAACAA
TCGTGCGCTATATAACATTAACGAATTTTTACTGCCAGAACATTTTTATGAACCATTACATGGCAAAATATACAAGTCAA
TTAATCTCATTATTAGTAAAGGAATTAGTGCTAGTGTAATTTCGCTCAAAAATATGCTAGGCAATGAACTCGCATTTGAG
GAAATAGGTGGAGTGGATTATCTAGCTAAACTTACAACTTTAGCATTAAGTATAGTTAATGTTAATGAGTACGGCAAAAT
AGTATATGATCTTGCGCTGCGGCGTTATTTTATTGAAATTGGAGAAAAAATAGTAACAAATGCATATTCTTCTACTTTAG
CAGATTTAGCTATAACTCAGATCGAAACTGCTGAATCTCAATTATATGATCTAGGTTCAAGAGGAACTTTAAGTAAAGGA
TTTACAAAATTACAAACTTCAATTGAAGAATCATGGACATCAATTTCATCTGCTATTAAAAATAAAAACTCTATTAACGG
TATTAGTAGTGGACTACTTGACCTTGATTCAAAGCTTGGAGGATTTAAAAATTCTGACCTAATAATATTAGCTGGCAGAC
CTTCAATGGGTAAAACTGCTTTAGGAGTTAACTTAGCAATAAATGCTTGTAAATATTTTTTATCATCATCTACTCAACAA
AATAGCAAAGTTTCTAATATAACACCATCAGTTGGATTCTTTTCTTTAGAAATGTCATCTCAGCAAATCTCAACTCGAAT
TCTTTCTATAGAATCAGAAATTAACAGCTCTGCATTATTTAACGGTAAAATAGATGAACAAGATGTTGATAAGTTGAAGA
CTGTACAAAATGAAATACAAAAGTGGAATTTTTTTATAGATGATGCTCCAGCAATCTCGATATCTGCAATTAGATCTCGA
GCTCGTAGACTTAAACGTACTCATAATTTAGCAATATTATTTGTTGATTATTTACAGCTAATAAAAATTGATAACAGAAG
AAGTCAGTATAATAGAGTGCAGGAGATTTCTGAAATTACTCAGAGCTTAAAAGATCTTGCTAAAGAGCTGAATATTTCAG
TAATTGCATTATCTCAATTGTCTAGAGCTTGTAGAACAAATGTCAGATAA

Upstream 100 bases:

>100_bases
GAGAGAACTATCTGAAAGATCTTGAGCCCGCTTTTAAAGCTCAAGGATTTTCTTTCGAGTTAGTTAAGTTTAGTAATTTT
AATAAAATTTAAAGGGTGAT

Downstream 100 bases:

>100_bases
AAAGCCTATTCTCTCAGATCTAAGAGAATCAGGCTCAATTGAACAAGACGCCGATATTGTAATGCTCATATATCGTGACG
AATATTACTTGTCTAGATCA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISASVISLKNMLGNELAFE
EIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYFIEIGEKIVTNAYSSTLADLAITQIETAESQLYDLGSRGTLSKG
FTKLQTSIEESWTSISSAIKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLSSSTQQ
NSKVSNITPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIDEQDVDKLKTVQNEIQKWNFFIDDAPAISISAIRSR
ARRLKRTHNLAILFVDYLQLIKIDNRRSQYNRVQEISEITQSLKDLAKELNISVIALSQLSRACRTNVR

Sequences:

>Translated_389_residues
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISASVISLKNMLGNELAFE
EIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYFIEIGEKIVTNAYSSTLADLAITQIETAESQLYDLGSRGTLSKG
FTKLQTSIEESWTSISSAIKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLSSSTQQ
NSKVSNITPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIDEQDVDKLKTVQNEIQKWNFFIDDAPAISISAIRSR
ARRLKRTHNLAILFVDYLQLIKIDNRRSQYNRVQEISEITQSLKDLAKELNISVIALSQLSRACRTNVR
>Mature_389_residues
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISASVISLKNMLGNELAFE
EIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYFIEIGEKIVTNAYSSTLADLAITQIETAESQLYDLGSRGTLSKG
FTKLQTSIEESWTSISSAIKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLSSSTQQ
NSKVSNITPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIDEQDVDKLKTVQNEIQKWNFFIDDAPAISISAIRSR
ARRLKRTHNLAILFVDYLQLIKIDNRRSQYNRVQEISEITQSLKDLAKELNISVIALSQLSRACRTNVR

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=385, Percent_Identity=31.9480519480519, Blast_Score=194, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 43145; Mature: 43145

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISK
CCCCHHHHCCCCCCHHHHHHHHHHCCCCEEECHHHHHCCHHHHCCHHHHHHHHHHHHHHC
GISASVISLKNMLGNELAFEEIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYFIEI
CCCHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GEKIVTNAYSSTLADLAITQIETAESQLYDLGSRGTLSKGFTKLQTSIEESWTSISSAIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLSSSTQQ
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCC
NSKVSNITPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIDEQDVDKLKTVQNEIQ
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHH
KWNFFIDDAPAISISAIRSRARRLKRTHNLAILFVDYLQLIKIDNRRSQYNRVQEISEIT
HCCEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
QSLKDLAKELNISVIALSQLSRACRTNVR
HHHHHHHHHCCCCEEEHHHHHHHHHCCCC
>Mature Secondary Structure
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISK
CCCCHHHHCCCCCCHHHHHHHHHHCCCCEEECHHHHHCCHHHHCCHHHHHHHHHHHHHHC
GISASVISLKNMLGNELAFEEIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYFIEI
CCCHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GEKIVTNAYSSTLADLAITQIETAESQLYDLGSRGTLSKGFTKLQTSIEESWTSISSAIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLSSSTQQ
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCC
NSKVSNITPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIDEQDVDKLKTVQNEIQ
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHH
KWNFFIDDAPAISISAIRSRARRLKRTHNLAILFVDYLQLIKIDNRRSQYNRVQEISEIT
HCCEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
QSLKDLAKELNISVIALSQLSRACRTNVR
HHHHHHHHHCCCCEEEHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA