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The map label for this gene is traG [H]

Identifier: 189183110

GI number: 189183110

Start: 195607

End: 196806

Strand: Reverse

Name: traG [H]

Synonym: OTT_0203

Alternate gene names: 189183110

Gene position: 196806-195607 (Counterclockwise)

Preceding gene: 189183111

Following gene: 189183109

Centisome position: 9.8

GC content: 35.75

Gene sequence:

>1200_bases
ATGTTTGCTTCTCTTTCCTCGCAAACGAGTTATTTTGTATCTAAAATGTTGGAAAATCATCTTTTACCGGCTTATGAAGG
ACTATCGAGCAGAAAAACTGGTATTATGTTTGGAGCTAAGGCTGTAGCTAAGATTCGAGATGTGCAAATACATGATCCAG
TAACTTTAACTAATACTAAGGAATTTCTTAGACAATGCTTTATGAAGCCTTACATCATAGGTAATATCTTAGGTAAAAAA
GCAGCTGCTCAACAAACTAATGATATCATAGGATTTATCGAGCAGAATATACCTAATAATTTCGGTATTTATTATCGCGA
ACCTAGTAATTTAGGTATTAGTTTCAAGACATGCAGACAAGCTACTCCATTAATTAAGGCAGCAATTCATAAAGAATTAA
ACGAAGGACTTCTAACCAAGTTTGCAGCTGCGATTGGAGTTCAATCTGATCAGTCACACATGTTAAGTCAAAGACTAAAA
GTCATGACTGGAGATACACTAAAATATCTACAAAGAGAACAACAAGACATTCATGAATGGATGAAGCAAGCTATGCTACT
TAATGCTAATCGTGAGTCATATGATGACTGGCGTGAGAAGTTTTCATTATCACGAATTTATCCTAATCTGGTGAGTATGC
ATGCAATCAGAGGGCTCTTTCAAAAATCATTTTCGTATCTAGTCGCTGGGGAAATGGCTGCTCACATGATGCCTATTTTA
CAATCTGTTTTTTTTGCATTAGTAGTCTCAATGATCTTTATTGTATTTCCAATGGGCCTATTACCTGGCGGCTATAACAT
ACTCAAAACATGGATTTTGTTAATAATATGGGTCAGCAGCTGGCCAGTATTTTTTACAATAATCCATTGTCTAGGAATGA
TCAGCTTATCCAGTAAATCTGGAGCTTTCGGTAGTGATTATGGTCTGAATATGCTATCGCAAGGAAGCTTTGCAGAGATG
ATTTTATATAGCTATGCGACATTTCAAATGCTTGCATCATCAATACCAATGCTTTCTTGGTCAGTGATAAAAGCTTGTGC
TCATGCTACAGCTAACTTAGCCAGCCAGTTCTCTCCTATACAAGTTGCTAGCAGCTTAGGAAGTAATATAGTCGACAATA
ACTTGAGTAAAGAATTCGAGAATTTTACTATTGCAAAAGACTATGAGCAAAAACTACAGATTTTGAAAAAATACTCTTGA

Upstream 100 bases:

>100_bases
TTAATCAATCAGAAATAATAATTTTATAAAAACTTACATCTTTTTTATAAAATTATTATTTCTTTTCTTGATTCTTATCA
CGGTATCTATAGGTATTGCA

Downstream 100 bases:

>100_bases
TATAGATGAATTTTCAGAATCAAGGAAATTTCACCAGGGGCTCGCAGTTATTTGCCCATAAGTTAAGAATGTTTGGGCAA
GGTAGTATCAATGTTTTTAC

Product: putative conjugative transfer protein TraG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MFASLSSQTSYFVSKMLENHLLPAYEGLSSRKTGIMFGAKAVAKIRDVQIHDPVTLTNTKEFLRQCFMKPYIIGNILGKK
AAAQQTNDIIGFIEQNIPNNFGIYYREPSNLGISFKTCRQATPLIKAAIHKELNEGLLTKFAAAIGVQSDQSHMLSQRLK
VMTGDTLKYLQREQQDIHEWMKQAMLLNANRESYDDWREKFSLSRIYPNLVSMHAIRGLFQKSFSYLVAGEMAAHMMPIL
QSVFFALVVSMIFIVFPMGLLPGGYNILKTWILLIIWVSSWPVFFTIIHCLGMISLSSKSGAFGSDYGLNMLSQGSFAEM
ILYSYATFQMLASSIPMLSWSVIKACAHATANLASQFSPIQVASSLGSNIVDNNLSKEFENFTIAKDYEQKLQILKKYS

Sequences:

>Translated_399_residues
MFASLSSQTSYFVSKMLENHLLPAYEGLSSRKTGIMFGAKAVAKIRDVQIHDPVTLTNTKEFLRQCFMKPYIIGNILGKK
AAAQQTNDIIGFIEQNIPNNFGIYYREPSNLGISFKTCRQATPLIKAAIHKELNEGLLTKFAAAIGVQSDQSHMLSQRLK
VMTGDTLKYLQREQQDIHEWMKQAMLLNANRESYDDWREKFSLSRIYPNLVSMHAIRGLFQKSFSYLVAGEMAAHMMPIL
QSVFFALVVSMIFIVFPMGLLPGGYNILKTWILLIIWVSSWPVFFTIIHCLGMISLSSKSGAFGSDYGLNMLSQGSFAEM
ILYSYATFQMLASSIPMLSWSVIKACAHATANLASQFSPIQVASSLGSNIVDNNLSKEFENFTIAKDYEQKLQILKKYS
>Mature_399_residues
MFASLSSQTSYFVSKMLENHLLPAYEGLSSRKTGIMFGAKAVAKIRDVQIHDPVTLTNTKEFLRQCFMKPYIIGNILGKK
AAAQQTNDIIGFIEQNIPNNFGIYYREPSNLGISFKTCRQATPLIKAAIHKELNEGLLTKFAAAIGVQSDQSHMLSQRLK
VMTGDTLKYLQREQQDIHEWMKQAMLLNANRESYDDWREKFSLSRIYPNLVSMHAIRGLFQKSFSYLVAGEMAAHMMPIL
QSVFFALVVSMIFIVFPMGLLPGGYNILKTWILLIIWVSSWPVFFTIIHCLGMISLSSKSGAFGSDYGLNMLSQGSFAEM
ILYSYATFQMLASSIPMLSWSVIKACAHATANLASQFSPIQVASSLGSNIVDNNLSKEFENFTIAKDYEQKLQILKKYS

Specific function: Plays a crucial role in donor-recipient cell interactions. Required for two stages of the conjugation process:pilus biosynthesis and mating aggregate stabilization. May interact with traN [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012931 [H]

Pfam domain/function: PF07916 TraG_N [H]

EC number: NA

Molecular weight: Translated: 44875; Mature: 44875

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCHHHHCHHHHHHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHCCHHHHCHHHHHHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1348105; 7915817 [H]