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The map label for this gene is traD [H]

Identifier: 189182960

GI number: 189182960

Start: 58207

End: 59799

Strand: Reverse

Name: traD [H]

Synonym: OTT_0053

Alternate gene names: 189182960

Gene position: 59799-58207 (Counterclockwise)

Preceding gene: 189182961

Following gene: 189182958

Centisome position: 2.98

GC content: 32.96

Gene sequence:

>1593_bases
TTGTATTATTTTGTAATTGAAAGATATGTGCAGCTTAAGCTAGCAATTGGTGAGCATTTTTATGATATCGATCAAATAGG
CATTAAGTTTTATAGTTTAAGGTTTAAAAAATGGATGCACCTCAATGCTCAAGATTTTTTGCATGAGTTTTATACAGGCC
AACATGGATTTAAAATACAGCAATTATTGGAATTCTTAATTAATTCAGCATTGTTAGAAAGCTTAGTTGTTTTTACTATT
GGTGTAATAATTTCAATTGTTTTCTTTACAGCTCAAGGCAAAAACACGATTATTAAGGCCAAAATTAGAGGCGCTGATTT
TGTGGAGTATAAATACCTATCTAAAATGCTAAAAAGCGCTAAAAAGGCCTCAAAAATCTGTTTTGGAGGCTTGCCATTAG
TAAAGAATAGTAAAAGGCTGCACATTCTTATTACTGGAACAACAGGTACTGGTAAAACTAATATGCTTAATGAACTGCTG
CCACAAATTCGATTACACAAAGACCGAGCAATAATTGTAGACACAACTGGAGCTTTTACTGATAGATTTTTTGACTCTAA
ATGTGATAAGCTGCTTAATCCTTTGGAAAAAAATAGTGAGCAATGGTTGCCTTGGAATGATTGTTTTGAAGCAGCTGATT
TTCATGATATAGCTAGTAGTTTTAGTAATTATACTCCTAAACTTGATGACTTTTTTGCTAAAAATGCTGAATTAGTCTTA
TCTGAAGCATTGAAGCTATATCAGGATGATAAAGATATCATAAAACTAATTCATACAATCATTTACTCTGATAATAGACA
ATTTGCAAAAGCTTTTAGAAACACAGCTGTATCAGGTATTATAAGCGAAAGTGCGCTTGAAACTTCTGCAGGAATTCAGT
CTACGCTTGGAAAGAATATTACTTCGCTACAATATTTAAAGCCTGGAGGTAGTTTTAGCATAAAAGAATGGTTTAGTAAT
TCAAATGAAACTAGCTGGCTATTTATCACAGCTAACCCAAATCAAAGAGCTACTTTATGCCCACTTATTTCAGCATGGAT
AAGCATAGCTATCAAAGCTTTGATGTGTAGAAATCCTAATCATGATAACAAAAACATGTGGTTTATACTTGATGAATTGC
CAGCTCTACAAAAAGTTTCGTCTTTACCAGTTGCTTTGGCTGAAAGTAGAAAGTATGGAGGCTGCTTTGTTGCTGGATTG
CAGAACATTCATCAATTAGAAGCAATATATGGGGCTGCTGAATGTGCTTCTATGCTGGATTTGTTTAATAGTAAATTTAT
TTTTCGAGTTAGCGATCAGGTTACAGCTTATAAGTCAGCATTAACACTAGGTGAGCAAGAAATTATTGAAACTCAAGAAA
ACTTGTCATATGGATCAAATACTATGCGAGATGGAGTAAATATGAATAATGTTGAGCGTAAAAGGATTTTAGTTATGCCA
TCTGAAATTATGAACCTACCAGACCTTACATGTTATGTAAAGCTTGCCGGTAACTTTCCCATCACAAAACTAACTATGCA
GCTACAAAACTTAAATACAGCTTTTGTTTGGGGATATAAATTGCTCAAAAAACTTAAATTAGTAGAGTATTAA

Upstream 100 bases:

>100_bases
GAAATAGCCTTTTTATCTTCGATTGGAGATATATTTCCAATTTATGATTATATCACATTAGAATCTATTTCTGGTATATC
AAAAAGTATTTCTGAGCAGT

Downstream 100 bases:

>100_bases
TTCGAAAAATTAATACTCTATGTTATTTTTTAAATTAATCACTTCTTCTTTTGACAATCCAGTATTTTCAGAAATAAATT
CAACTGAAAAGCCAGCTTTT

Product: putative conjugative transfer protein TraD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MYYFVIERYVQLKLAIGEHFYDIDQIGIKFYSLRFKKWMHLNAQDFLHEFYTGQHGFKIQQLLEFLINSALLESLVVFTI
GVIISIVFFTAQGKNTIIKAKIRGADFVEYKYLSKMLKSAKKASKICFGGLPLVKNSKRLHILITGTTGTGKTNMLNELL
PQIRLHKDRAIIVDTTGAFTDRFFDSKCDKLLNPLEKNSEQWLPWNDCFEAADFHDIASSFSNYTPKLDDFFAKNAELVL
SEALKLYQDDKDIIKLIHTIIYSDNRQFAKAFRNTAVSGIISESALETSAGIQSTLGKNITSLQYLKPGGSFSIKEWFSN
SNETSWLFITANPNQRATLCPLISAWISIAIKALMCRNPNHDNKNMWFILDELPALQKVSSLPVALAESRKYGGCFVAGL
QNIHQLEAIYGAAECASMLDLFNSKFIFRVSDQVTAYKSALTLGEQEIIETQENLSYGSNTMRDGVNMNNVERKRILVMP
SEIMNLPDLTCYVKLAGNFPITKLTMQLQNLNTAFVWGYKLLKKLKLVEY

Sequences:

>Translated_530_residues
MYYFVIERYVQLKLAIGEHFYDIDQIGIKFYSLRFKKWMHLNAQDFLHEFYTGQHGFKIQQLLEFLINSALLESLVVFTI
GVIISIVFFTAQGKNTIIKAKIRGADFVEYKYLSKMLKSAKKASKICFGGLPLVKNSKRLHILITGTTGTGKTNMLNELL
PQIRLHKDRAIIVDTTGAFTDRFFDSKCDKLLNPLEKNSEQWLPWNDCFEAADFHDIASSFSNYTPKLDDFFAKNAELVL
SEALKLYQDDKDIIKLIHTIIYSDNRQFAKAFRNTAVSGIISESALETSAGIQSTLGKNITSLQYLKPGGSFSIKEWFSN
SNETSWLFITANPNQRATLCPLISAWISIAIKALMCRNPNHDNKNMWFILDELPALQKVSSLPVALAESRKYGGCFVAGL
QNIHQLEAIYGAAECASMLDLFNSKFIFRVSDQVTAYKSALTLGEQEIIETQENLSYGSNTMRDGVNMNNVERKRILVMP
SEIMNLPDLTCYVKLAGNFPITKLTMQLQNLNTAFVWGYKLLKKLKLVEY
>Mature_530_residues
MYYFVIERYVQLKLAIGEHFYDIDQIGIKFYSLRFKKWMHLNAQDFLHEFYTGQHGFKIQQLLEFLINSALLESLVVFTI
GVIISIVFFTAQGKNTIIKAKIRGADFVEYKYLSKMLKSAKKASKICFGGLPLVKNSKRLHILITGTTGTGKTNMLNELL
PQIRLHKDRAIIVDTTGAFTDRFFDSKCDKLLNPLEKNSEQWLPWNDCFEAADFHDIASSFSNYTPKLDDFFAKNAELVL
SEALKLYQDDKDIIKLIHTIIYSDNRQFAKAFRNTAVSGIISESALETSAGIQSTLGKNITSLQYLKPGGSFSIKEWFSN
SNETSWLFITANPNQRATLCPLISAWISIAIKALMCRNPNHDNKNMWFILDELPALQKVSSLPVALAESRKYGGCFVAGL
QNIHQLEAIYGAAECASMLDLFNSKFIFRVSDQVTAYKSALTLGEQEIIETQENLSYGSNTMRDGVNMNNVERKRILVMP
SEIMNLPDLTCYVKLAGNFPITKLTMQLQNLNTAFVWGYKLLKKLKLVEY

Specific function: TraD is a cell envelope protein. The function of traD is unknown but it is essential for DNA transfer. It may form or may be part of a non-specific pore for DNA export, or it may directly energize DNA transport [H]

COG id: COG3505

COG function: function code U; Type IV secretory pathway, VirD4 components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To traD of plasmid IncFII R100 [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014128
- InterPro:   IPR019476
- InterPro:   IPR022585 [H]

Pfam domain/function: PF12615 TraD_N; PF10412 TrwB_AAD_bind [H]

EC number: NA

Molecular weight: Translated: 60210; Mature: 60210

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYYFVIERYVQLKLAIGEHFYDIDQIGIKFYSLRFKKWMHLNAQDFLHEFYTGQHGFKIQ
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHH
QLLEFLINSALLESLVVFTIGVIISIVFFTAQGKNTIIKAKIRGADFVEYKYLSKMLKSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHH
KKASKICFGGLPLVKNSKRLHILITGTTGTGKTNMLNELLPQIRLHKDRAIIVDTTGAFT
HHHHHHEECCCCEEECCCEEEEEEEECCCCCCHHHHHHHHHHHEECCCCEEEEECCCCHH
DRFFDSKCDKLLNPLEKNSEQWLPWNDCFEAADFHDIASSFSNYTPKLDDFFAKNAELVL
HHHHHHHHHHHHCHHHCCCCCCCCHHHHHHHCHHHHHHHHHHCCCCCHHHHHHCCHHHHH
SEALKLYQDDKDIIKLIHTIIYSDNRQFAKAFRNTAVSGIISESALETSAGIQSTLGKNI
HHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCC
TSLQYLKPGGSFSIKEWFSNSNETSWLFITANPNQRATLCPLISAWISIAIKALMCRNPN
CEEEEECCCCCCCHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC
HDNKNMWFILDELPALQKVSSLPVALAESRKYGGCFVAGLQNIHQLEAIYGAAECASMLD
CCCCEEEEEECCCCHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LFNSKFIFRVSDQVTAYKSALTLGEQEIIETQENLSYGSNTMRDGVNMNNVERKRILVMP
HHCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCEEEEEC
SEIMNLPDLTCYVKLAGNFPITKLTMQLQNLNTAFVWGYKLLKKLKLVEY
HHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYYFVIERYVQLKLAIGEHFYDIDQIGIKFYSLRFKKWMHLNAQDFLHEFYTGQHGFKIQ
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHH
QLLEFLINSALLESLVVFTIGVIISIVFFTAQGKNTIIKAKIRGADFVEYKYLSKMLKSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHH
KKASKICFGGLPLVKNSKRLHILITGTTGTGKTNMLNELLPQIRLHKDRAIIVDTTGAFT
HHHHHHEECCCCEEECCCEEEEEEEECCCCCCHHHHHHHHHHHEECCCCEEEEECCCCHH
DRFFDSKCDKLLNPLEKNSEQWLPWNDCFEAADFHDIASSFSNYTPKLDDFFAKNAELVL
HHHHHHHHHHHHCHHHCCCCCCCCHHHHHHHCHHHHHHHHHHCCCCCHHHHHHCCHHHHH
SEALKLYQDDKDIIKLIHTIIYSDNRQFAKAFRNTAVSGIISESALETSAGIQSTLGKNI
HHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCC
TSLQYLKPGGSFSIKEWFSNSNETSWLFITANPNQRATLCPLISAWISIAIKALMCRNPN
CEEEEECCCCCCCHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC
HDNKNMWFILDELPALQKVSSLPVALAESRKYGGCFVAGLQNIHQLEAIYGAAECASMLD
CCCCEEEEEECCCCHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LFNSKFIFRVSDQVTAYKSALTLGEQEIIETQENLSYGSNTMRDGVNMNNVERKRILVMP
HHCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCEEEEEC
SEIMNLPDLTCYVKLAGNFPITKLTMQLQNLNTAFVWGYKLLKKLKLVEY
HHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2680768; 7915817; 10464182; 3323526; 2164585; 2163400; 1618779; 10498725 [H]