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The map label for this gene is virB4 [H]

Identifier: 189182909

GI number: 189182909

Start: 4977

End: 7430

Strand: Reverse

Name: virB4 [H]

Synonym: OTT_0002

Alternate gene names: 189182909

Gene position: 7430-4977 (Counterclockwise)

Preceding gene: 189182914

Following gene: 189182908

Centisome position: 0.37

GC content: 29.14

Gene sequence:

>2454_bases
ATGGGGTTTATACATAAATTTTTACATAGTAGAAAAATAGATGCAAAACAGTTTGATAGCAATGCTCAGAACTTTATTCC
TATAACTTGCCACTACAATGATGAAACTTTATTAACTAAAAATGGCGAATTAATTCAAATATTACAAATAAAAGGTATTA
ATTCTGAATATACTAATAAGGATTTATTGGGTATCAGAGCAGCAATACGTAATGCTATTAAAAATCATATTAATAATAGC
AACTTTGCAGTTTGGATACATGTTGTAAGGCGCTGTGATAATATTGATGACTCTACTCCTTACAGTCAAGCATTGCCAAA
TTTTATTCATAACATTTGGAGCAAAAAAAACTACTGGCAAGATAAATTTGTTAGTACATTATATATCTCAATTGTGCATC
ATGGATGTAACTTAAATATTAGAAACTTAGATAGTTTTTTGAACTCTTTATCGTTTAAGACATTGGATAGTTTTTATGAT
AAGTTCATAGCATCTGCATTAAAAAATTTATCCATGGCTATAAATAATATAATAACTGAGCTGCAAGGTTATAATCCATG
CAAGTTAAGTATTAAAATTGATAATAATAAAGTATATTCTGACCTTTTAAATTTTTATAAAAAAATAATACAACTTAATA
CTGGGTTAATAGAAGTAAAAGATGTTGATTGTGCTGTACAGTTAGCCACTCATAAATATTCTTTTGGAGGAGACAAAATA
GAGGTTATACATAAACAAGAAAAAAAGTTTGCATCTATTTTAGGTATCAAGGAATATCAAGATATTAAAGATGAATTAGT
GAGTAAGTGCTTACATGCTCCAGTCGAATTTATTGCTACAGAAATTTTTTATTTTGCTGACCAAGTAAAAGCTATTAATA
GTTACTCTCAACAAAGTAAAGTTATTAGTGCAAGTCGTGACAGTGAACTAGCTCTATTCAAAGGAATAGAGCCAATTGTG
AATTCTGATAACACATTACCACAGTTTTGCCATCAACAAATTAGCATAATGATTATGGCTGATAGCATTGATCTGCTTGA
GAGTAGAGTAGCTTATATGTCGCAAAGATTAGTAAAACAAGGTATAATACATGTTCGAGAGGATATTAATCTAGCGCAAA
CATTTTGGTCTCAGTTACCAGGTAATTTTAATTACATTAGACGTGTTTCTTATAATATTATTGATAATGTAGCTGCTTTA
GCAGCACTACATCATTACCCTGTTGGCATGCAGCATAGTCTATGGGGTAGATCTGTCACTATATTTCGAACAGAATGCGG
TACTCCTTATTTCATGAATTTTCATGATGAGAGTGCAAAAGGGCATACTTGTATAATTGGTGGAGCTCAATCTGGTCGAG
CTACTATCGCTAATTTTTTATTGTCTGAAGCATCAAAATATTCTCCAACTATACTTTATTTAACTAATAGAAATAATGCT
CAAGTTTTTATAAAAGGAATAGAAGGAACATGGCACGATGGTGATTTACCATTTAATCCATTAAGCTACTTAGAAAGCTC
TGATGACTTATTAGAACTATTTAAGATAATAACAGGTAATTATTTTAATAATTTAACTGATAGTGAAATTGAATGCATTA
CAGAGTTAGCTATTGCAGTTATGGATATGCCACCTGAATCGCGTTCTTTGATTAAAATTATCAAAGATTTTAATTTTAAG
GCTTATAGTGCTGGAGCTAGTGTAAAGAAGCGTATTAAGTTATTTATTAGTGATGATAAATGTAGTAAAATTTTTAATCA
ATCTTCTACTTTAGAATTGCAAGATAATAATGTAACTGCTATAAACTTAGCTGATTATACTGATTATCAATTTAATCAAT
CTTATACACCTCAAGTAGGAGAAAAGCCGGAAATTTATCATAACAAATTACAAACTTTGCACTGTCTAAGGTCTGGAATT
ATATTTTCTTTAATAAAAAAATTCAGCTTACTAGCCTCTCCAGAGCCTAAAATACTAGTTGTTGATAATATGCCTGATTT
AATAGTAGAGCAAGTGTTTATGAACTTAATTAAACAAATCACTGAACAACTATCTGAAAATAATGGAATAATTCTTTCTG
TTGTTCAAGTAAATCAGAATGATCAATTTTATTATTCTGATTTCTGGAAGAAATGGTTAAAGTTTAATAATACTAAAATT
ATACTACCAACTAATATAAGAAATTTACCTATTGCTGAAATCTGTAACCTAAGTACTAGAGAAGCTAAAAAATTTAATAC
TATTTCACCAAATTCTAGATTATTTATGGTAAAACAAGATAATAATTATGTTATTGTAGAACTTAATTTGGAAGGATTTC
CAGCAATTAGAAAGCTTCTATCAGCTGAAGAAGAAGATTTGAATCTTTTTCATAAAATTATGCAAAAAATAGGCGATGAT
GCTCCTTCAGCTTGGTTAAGTGATGTTTATGATCAGTTTCAGAACAGTGAGTAA

Upstream 100 bases:

>100_bases
TGTAATATTTACATTTTTTATTTAATTCTTGAAATGAAAATGTAGTAAATATCATATTAAATTAATATAGTATGCTAATA
ACAATATAAGCTTATAATTT

Downstream 100 bases:

>100_bases
TGAAGCCACAACATATCTTTTTAGTTATAATTGTTGCTATAATTAATTTTATAACTGTTGCTAATGCAACAGCTTCGATT
TGTACATGGTTTCGTGGAAA

Product: type IV secretion system protein VirB4

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 817; Mature: 816

Protein sequence:

>817_residues
MGFIHKFLHSRKIDAKQFDSNAQNFIPITCHYNDETLLTKNGELIQILQIKGINSEYTNKDLLGIRAAIRNAIKNHINNS
NFAVWIHVVRRCDNIDDSTPYSQALPNFIHNIWSKKNYWQDKFVSTLYISIVHHGCNLNIRNLDSFLNSLSFKTLDSFYD
KFIASALKNLSMAINNIITELQGYNPCKLSIKIDNNKVYSDLLNFYKKIIQLNTGLIEVKDVDCAVQLATHKYSFGGDKI
EVIHKQEKKFASILGIKEYQDIKDELVSKCLHAPVEFIATEIFYFADQVKAINSYSQQSKVISASRDSELALFKGIEPIV
NSDNTLPQFCHQQISIMIMADSIDLLESRVAYMSQRLVKQGIIHVREDINLAQTFWSQLPGNFNYIRRVSYNIIDNVAAL
AALHHYPVGMQHSLWGRSVTIFRTECGTPYFMNFHDESAKGHTCIIGGAQSGRATIANFLLSEASKYSPTILYLTNRNNA
QVFIKGIEGTWHDGDLPFNPLSYLESSDDLLELFKIITGNYFNNLTDSEIECITELAIAVMDMPPESRSLIKIIKDFNFK
AYSAGASVKKRIKLFISDDKCSKIFNQSSTLELQDNNVTAINLADYTDYQFNQSYTPQVGEKPEIYHNKLQTLHCLRSGI
IFSLIKKFSLLASPEPKILVVDNMPDLIVEQVFMNLIKQITEQLSENNGIILSVVQVNQNDQFYYSDFWKKWLKFNNTKI
ILPTNIRNLPIAEICNLSTREAKKFNTISPNSRLFMVKQDNNYVIVELNLEGFPAIRKLLSAEEEDLNLFHKIMQKIGDD
APSAWLSDVYDQFQNSE

Sequences:

>Translated_817_residues
MGFIHKFLHSRKIDAKQFDSNAQNFIPITCHYNDETLLTKNGELIQILQIKGINSEYTNKDLLGIRAAIRNAIKNHINNS
NFAVWIHVVRRCDNIDDSTPYSQALPNFIHNIWSKKNYWQDKFVSTLYISIVHHGCNLNIRNLDSFLNSLSFKTLDSFYD
KFIASALKNLSMAINNIITELQGYNPCKLSIKIDNNKVYSDLLNFYKKIIQLNTGLIEVKDVDCAVQLATHKYSFGGDKI
EVIHKQEKKFASILGIKEYQDIKDELVSKCLHAPVEFIATEIFYFADQVKAINSYSQQSKVISASRDSELALFKGIEPIV
NSDNTLPQFCHQQISIMIMADSIDLLESRVAYMSQRLVKQGIIHVREDINLAQTFWSQLPGNFNYIRRVSYNIIDNVAAL
AALHHYPVGMQHSLWGRSVTIFRTECGTPYFMNFHDESAKGHTCIIGGAQSGRATIANFLLSEASKYSPTILYLTNRNNA
QVFIKGIEGTWHDGDLPFNPLSYLESSDDLLELFKIITGNYFNNLTDSEIECITELAIAVMDMPPESRSLIKIIKDFNFK
AYSAGASVKKRIKLFISDDKCSKIFNQSSTLELQDNNVTAINLADYTDYQFNQSYTPQVGEKPEIYHNKLQTLHCLRSGI
IFSLIKKFSLLASPEPKILVVDNMPDLIVEQVFMNLIKQITEQLSENNGIILSVVQVNQNDQFYYSDFWKKWLKFNNTKI
ILPTNIRNLPIAEICNLSTREAKKFNTISPNSRLFMVKQDNNYVIVELNLEGFPAIRKLLSAEEEDLNLFHKIMQKIGDD
APSAWLSDVYDQFQNSE
>Mature_816_residues
GFIHKFLHSRKIDAKQFDSNAQNFIPITCHYNDETLLTKNGELIQILQIKGINSEYTNKDLLGIRAAIRNAIKNHINNSN
FAVWIHVVRRCDNIDDSTPYSQALPNFIHNIWSKKNYWQDKFVSTLYISIVHHGCNLNIRNLDSFLNSLSFKTLDSFYDK
FIASALKNLSMAINNIITELQGYNPCKLSIKIDNNKVYSDLLNFYKKIIQLNTGLIEVKDVDCAVQLATHKYSFGGDKIE
VIHKQEKKFASILGIKEYQDIKDELVSKCLHAPVEFIATEIFYFADQVKAINSYSQQSKVISASRDSELALFKGIEPIVN
SDNTLPQFCHQQISIMIMADSIDLLESRVAYMSQRLVKQGIIHVREDINLAQTFWSQLPGNFNYIRRVSYNIIDNVAALA
ALHHYPVGMQHSLWGRSVTIFRTECGTPYFMNFHDESAKGHTCIIGGAQSGRATIANFLLSEASKYSPTILYLTNRNNAQ
VFIKGIEGTWHDGDLPFNPLSYLESSDDLLELFKIITGNYFNNLTDSEIECITELAIAVMDMPPESRSLIKIIKDFNFKA
YSAGASVKKRIKLFISDDKCSKIFNQSSTLELQDNNVTAINLADYTDYQFNQSYTPQVGEKPEIYHNKLQTLHCLRSGII
FSLIKKFSLLASPEPKILVVDNMPDLIVEQVFMNLIKQITEQLSENNGIILSVVQVNQNDQFYYSDFWKKWLKFNNTKII
LPTNIRNLPIAEICNLSTREAKKFNTISPNSRLFMVKQDNNYVIVELNLEGFPAIRKLLSAEEEDLNLFHKIMQKIGDDA
PSAWLSDVYDQFQNSE

Specific function: The type IV secretion system VirB/VirD4 is a major virulence determinant for subversion of human endothelial cell (HEC) function. VirB-dependent changes of HEC include massive cytoskeletal rearrangements, a proinflammatory activation by nuclear factor NF-

COG id: COG3451

COG function: function code U; Type IV secretory pathway, VirB4 components

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trbE/virB4 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004346
- InterPro:   IPR018145 [H]

Pfam domain/function: PF03135 CagE_TrbE_VirB [H]

EC number: NA

Molecular weight: Translated: 93401; Mature: 93270

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFIHKFLHSRKIDAKQFDSNAQNFIPITCHYNDETLLTKNGELIQILQIKGINSEYTNK
CCHHHHHHHHCCCCHHHHCCCCCCEEEEEEEECCCEEEECCCCEEEEEEEECCCCCCCCC
DLLGIRAAIRNAIKNHINNSNFAVWIHVVRRCDNIDDSTPYSQALPNFIHNIWSKKNYWQ
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHH
DKFVSTLYISIVHHGCNLNIRNLDSFLNSLSFKTLDSFYDKFIASALKNLSMAINNIITE
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQGYNPCKLSIKIDNNKVYSDLLNFYKKIIQLNTGLIEVKDVDCAVQLATHKYSFGGDKI
HCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEHEEECCCCCEE
EVIHKQEKKFASILGIKEYQDIKDELVSKCLHAPVEFIATEIFYFADQVKAINSYSQQSK
EEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VISASRDSELALFKGIEPIVNSDNTLPQFCHQQISIMIMADSIDLLESRVAYMSQRLVKQ
HHHCCCCCCCHHHHCCCHHHCCCCCHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHHH
GIIHVREDINLAQTFWSQLPGNFNYIRRVSYNIIDNVAALAALHHYPVGMQHSLWGRSVT
HHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
IFRTECGTPYFMNFHDESAKGHTCIIGGAQSGRATIANFLLSEASKYSPTILYLTNRNNA
EEEECCCCCEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCC
QVFIKGIEGTWHDGDLPFNPLSYLESSDDLLELFKIITGNYFNNLTDSEIECITELAIAV
EEEEEECCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHHH
MDMPPESRSLIKIIKDFNFKAYSAGASVKKRIKLFISDDKCSKIFNQSSTLELQDNNVTA
HCCCCCHHHHHHHHHHCCCEEEECCHHHHEEEEEEECCCHHHHHHCCCCEEEEECCCEEE
INLADYTDYQFNQSYTPQVGEKPEIYHNKLQTLHCLRSGIIFSLIKKFSLLASPEPKILV
EEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
VDNMPDLIVEQVFMNLIKQITEQLSENNGIILSVVQVNQNDQFYYSDFWKKWLKFNNTKI
ECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCEE
ILPTNIRNLPIAEICNLSTREAKKFNTISPNSRLFMVKQDNNYVIVELNLEGFPAIRKLL
EEECCCCCCCHHHHHCCCHHHHHHCCCCCCCCEEEEEEECCCEEEEEECCCCCHHHHHHH
SAEEEDLNLFHKIMQKIGDDAPSAWLSDVYDQFQNSE
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GFIHKFLHSRKIDAKQFDSNAQNFIPITCHYNDETLLTKNGELIQILQIKGINSEYTNK
CHHHHHHHHCCCCHHHHCCCCCCEEEEEEEECCCEEEECCCCEEEEEEEECCCCCCCCC
DLLGIRAAIRNAIKNHINNSNFAVWIHVVRRCDNIDDSTPYSQALPNFIHNIWSKKNYWQ
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHH
DKFVSTLYISIVHHGCNLNIRNLDSFLNSLSFKTLDSFYDKFIASALKNLSMAINNIITE
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQGYNPCKLSIKIDNNKVYSDLLNFYKKIIQLNTGLIEVKDVDCAVQLATHKYSFGGDKI
HCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEHEEECCCCCEE
EVIHKQEKKFASILGIKEYQDIKDELVSKCLHAPVEFIATEIFYFADQVKAINSYSQQSK
EEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VISASRDSELALFKGIEPIVNSDNTLPQFCHQQISIMIMADSIDLLESRVAYMSQRLVKQ
HHHCCCCCCCHHHHCCCHHHCCCCCHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHHH
GIIHVREDINLAQTFWSQLPGNFNYIRRVSYNIIDNVAALAALHHYPVGMQHSLWGRSVT
HHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
IFRTECGTPYFMNFHDESAKGHTCIIGGAQSGRATIANFLLSEASKYSPTILYLTNRNNA
EEEECCCCCEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCC
QVFIKGIEGTWHDGDLPFNPLSYLESSDDLLELFKIITGNYFNNLTDSEIECITELAIAV
EEEEEECCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHHH
MDMPPESRSLIKIIKDFNFKAYSAGASVKKRIKLFISDDKCSKIFNQSSTLELQDNNVTA
HCCCCCHHHHHHHHHHCCCEEEECCHHHHEEEEEEECCCHHHHHHCCCCEEEEECCCEEE
INLADYTDYQFNQSYTPQVGEKPEIYHNKLQTLHCLRSGIIFSLIKKFSLLASPEPKILV
EEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
VDNMPDLIVEQVFMNLIKQITEQLSENNGIILSVVQVNQNDQFYYSDFWKKWLKFNNTKI
ECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCEE
ILPTNIRNLPIAEICNLSTREAKKFNTISPNSRLFMVKQDNNYVIVELNLEGFPAIRKLL
EEECCCCCCCHHHHHCCCHHHHHHCCCCCCCCEEEEEEECCCEEEEEECCCCCHHHHHHH
SAEEEDLNLFHKIMQKIGDDAPSAWLSDVYDQFQNSE
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10749166; 10882236; 7494028 [H]