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The map label for this gene is murD

Identifier: 189026126

GI number: 189026126

Start: 986325

End: 987923

Strand: Direct

Name: murD

Synonym: TPASS_0903

Alternate gene names: 189026126

Gene position: 986325-987923 (Clockwise)

Preceding gene: 189026100

Following gene: 189026127

Centisome position: 86.56

GC content: 57.72

Gene sequence:

>1599_bases
GTGGAGCAGGCGCGCGCACTTCTTCAAGGCAAAACGGTAACCATCATGGGCCTCGGCGTGCACGGCGGGGGCTGCGCGGC
AGCCTGCTTTTGTGCAGAAGCGGGAGCGCGCCTTACGGTTACCGACCTGCGAAATGCTGATGCGCTTACCCCTTCCCTGA
AAAGACTTCGTGCGTACCCGTCCATCCGCTTTACGCTCGGTGAACACCGGCTGGAAGATTTTGAAAACGCGCATGTTGTC
ATCAAAAATCCCATCGTGAAGGGTGCACACAACATCTACCTTTCCGCTGCTCAACGCGCCGGCGCGCGCATCGAAACGGA
TATCTCCCTTTTCCTACGCCTTTCTCCCGCCCCGCTGCTAGCAGTAAGCGGCAGCAAGGGAAAATCCTCCACTGCCAGCG
CCCTGTGCTACAGCTTGCGTGCGCTGGGTTTCCCCGCCTTTCTAGGGGGGAATAGCACAGTCAGCCCGCTGGAATTTGTG
CGTCACACAACGCCTGCAACACCAGTGGTTCTGGAACTGTCGAGCTGGCAGCTTGCAGACCTACGCGCAGTCGATGCACA
GGACCACACTGTACACCACGCCGGTCTGCTGCGCCCGGAGATTGCCATCATGACTCCCATTATGGCTGACCATCAAAACT
GGTATGCGGATATGGAAAGCTACGTGGCAGACAAACAGGTGCTATACGCACACCAGGGAACGCACGATACACTGCTGTGT
AACGCAGATGACGGGTGGGGACCCCGCTTTGCCTGCGAAGCGCAGAAAAACGGAGTGCGCGTCTTTTGGTATACGGCCCA
GTCGCCTGAGACCGCTTGCCGCGCGTGCACCCCGCGCCTCATGGAGAGAGCTCTATGGAGAGCGACGGACGGAACCTACT
GGGCACGTTTTGCAGAAGGTGACCGCGCCTGCATGCTCATCCCACCGCAGCTGCACGTACCGGGCCGTGTCCTCCAAACG
CAAGTGGCGAGTGCAGCGCTTGCAGCGCTCCTGTTCGCGCAGCGGCATTCTCTACCTCCTTCCTCCTGCCCCCCGTGTTT
TTGCGCACACTCCCATTCCCCCGCGTACGCGAATCACGCGTCCCCCCCAGACTACGCTTGTCCGTCCGCTCACAGTCCCT
TCCAAGAGCACACCCGGCGTCTTGCACAAGCGCTGGAGTCCTACACGGGCATCGAACACCGACTAGAGTTTTTTTACGAA
AAGGGGGGACTGCGTTTTTATAACGATTCTGCATCCACTGTACCTGAGGCAACAATTGCAGCGCTGGAAGCCTTTGATGA
AAGCGTGGTTCTTATCGTTGGCGGCACTGATAAGAATGCAGACTACCAACCCTTGGCACAGGCAGCAGCCAAGGCGCACG
CATTGTACTTGCTTGCAGGAAGTGCTACGGCACGGTTGCAACCACTTCTTCATGCAGCACAGGTACCTTTTTACGGACCG
TTTACTTCCCTTGAGGTACTCCTGCAGGATCTCCGGGCGCGGCAGAAAAGCCCAGGCGTGATCGTGTTCTCTCCTGGGGC
AGCGAGTTTTGAACTTTTCGCCCACGAGTTTGAGCGAGGTACTACCTTTAAATCCCAGGTGCGTATCATTTTTGAATAA

Upstream 100 bases:

>100_bases
GGTAGTAAAAAAGCAACAAAAAGTAAAGAGAAACCGTGCCGCGAGCACCGCACGCGTCTCTTGTATTGTCACGCGTTGCG
CGGCTACAATCGGCCGCATC

Downstream 100 bases:

>100_bases
GAACACCCAAGACAAAGGCACACCCGCTGCACTGCAGGTTTTCCTTCTCCCCCAATACAACCGTTACAGCGCAGAAATGA
CGCGTCACGTGCCGCTCCTC

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MEQARALLQGKTVTIMGLGVHGGGCAAACFCAEAGARLTVTDLRNADALTPSLKRLRAYPSIRFTLGEHRLEDFENAHVV
IKNPIVKGAHNIYLSAAQRAGARIETDISLFLRLSPAPLLAVSGSKGKSSTASALCYSLRALGFPAFLGGNSTVSPLEFV
RHTTPATPVVLELSSWQLADLRAVDAQDHTVHHAGLLRPEIAIMTPIMADHQNWYADMESYVADKQVLYAHQGTHDTLLC
NADDGWGPRFACEAQKNGVRVFWYTAQSPETACRACTPRLMERALWRATDGTYWARFAEGDRACMLIPPQLHVPGRVLQT
QVASAALAALLFAQRHSLPPSSCPPCFCAHSHSPAYANHASPPDYACPSAHSPFQEHTRRLAQALESYTGIEHRLEFFYE
KGGLRFYNDSASTVPEATIAALEAFDESVVLIVGGTDKNADYQPLAQAAAKAHALYLLAGSATARLQPLLHAAQVPFYGP
FTSLEVLLQDLRARQKSPGVIVFSPGAASFELFAHEFERGTTFKSQVRIIFE

Sequences:

>Translated_532_residues
MEQARALLQGKTVTIMGLGVHGGGCAAACFCAEAGARLTVTDLRNADALTPSLKRLRAYPSIRFTLGEHRLEDFENAHVV
IKNPIVKGAHNIYLSAAQRAGARIETDISLFLRLSPAPLLAVSGSKGKSSTASALCYSLRALGFPAFLGGNSTVSPLEFV
RHTTPATPVVLELSSWQLADLRAVDAQDHTVHHAGLLRPEIAIMTPIMADHQNWYADMESYVADKQVLYAHQGTHDTLLC
NADDGWGPRFACEAQKNGVRVFWYTAQSPETACRACTPRLMERALWRATDGTYWARFAEGDRACMLIPPQLHVPGRVLQT
QVASAALAALLFAQRHSLPPSSCPPCFCAHSHSPAYANHASPPDYACPSAHSPFQEHTRRLAQALESYTGIEHRLEFFYE
KGGLRFYNDSASTVPEATIAALEAFDESVVLIVGGTDKNADYQPLAQAAAKAHALYLLAGSATARLQPLLHAAQVPFYGP
FTSLEVLLQDLRARQKSPGVIVFSPGAASFELFAHEFERGTTFKSQVRIIFE
>Mature_532_residues
MEQARALLQGKTVTIMGLGVHGGGCAAACFCAEAGARLTVTDLRNADALTPSLKRLRAYPSIRFTLGEHRLEDFENAHVV
IKNPIVKGAHNIYLSAAQRAGARIETDISLFLRLSPAPLLAVSGSKGKSSTASALCYSLRALGFPAFLGGNSTVSPLEFV
RHTTPATPVVLELSSWQLADLRAVDAQDHTVHHAGLLRPEIAIMTPIMADHQNWYADMESYVADKQVLYAHQGTHDTLLC
NADDGWGPRFACEAQKNGVRVFWYTAQSPETACRACTPRLMERALWRATDGTYWARFAEGDRACMLIPPQLHVPGRVLQT
QVASAALAALLFAQRHSLPPSSCPPCFCAHSHSPAYANHASPPDYACPSAHSPFQEHTRRLAQALESYTGIEHRLEFFYE
KGGLRFYNDSASTVPEATIAALEAFDESVVLIVGGTDKNADYQPLAQAAAKAHALYLLAGSATARLQPLLHAAQVPFYGP
FTSLEVLLQDLRARQKSPGVIVFSPGAASFELFAHEFERGTTFKSQVRIIFE

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=237, Percent_Identity=29.957805907173, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_TREPA (O83873)

Other databases:

- EMBL:   AE000520
- PIR:   D71267
- RefSeq:   NP_219338.1
- ProteinModelPortal:   O83873
- SMR:   O83873
- IntAct:   O83873
- GeneID:   2611814
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0903
- NMPDR:   fig|243276.1.peg.897
- TIGR:   TP_0903
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   CLSK218917
- BioCyc:   TPAL243276:TP_0903-MONOMER
- BRENDA:   6.3.2.9
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 57848; Mature: 57848

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQARALLQGKTVTIMGLGVHGGGCAAACFCAEAGARLTVTDLRNADALTPSLKRLRAYP
CCHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHCCC
SIRFTLGEHRLEDFENAHVVIKNPIVKGAHNIYLSAAQRAGARIETDISLFLRLSPAPLL
CEEEEECHHHHHCCCCCEEEEECCCCCCCHHEEEEEHHHCCCEEEECEEEEEEECCCCEE
AVSGSKGKSSTASALCYSLRALGFPAFLGGNSTVSPLEFVRHTTPATPVVLELSSWQLAD
EEECCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHCCCCCCEEEEECCCCEEE
LRAVDAQDHTVHHAGLLRPEIAIMTPIMADHQNWYADMESYVADKQVLYAHQGTHDTLLC
EEEECCCCCEEHHHCCCCCCHHHCCCHHHCCHHHHHHHHHHHCCCEEEEEECCCCCEEEE
NADDGWGPRFACEAQKNGVRVFWYTAQSPETACRACTPRLMERALWRATDGTYWARFAEG
ECCCCCCCCEEEECCCCCEEEEEEECCCCHHHHHHCCHHHHHHHHHHCCCCCEEEEECCC
DRACMLIPPQLHVPGRVLQTQVASAALAALLFAQRHSLPPSSCPPCFCAHSHSPAYANHA
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCC
SPPDYACPSAHSPFQEHTRRLAQALESYTGIEHRLEFFYEKGGLRFYNDSASTVPEATIA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEECCCCCCCCHHHHH
ALEAFDESVVLIVGGTDKNADYQPLAQAAAKAHALYLLAGSATARLQPLLHAAQVPFYGP
HHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHCCCCCCC
FTSLEVLLQDLRARQKSPGVIVFSPGAASFELFAHEFERGTTFKSQVRIIFE
HHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCEEECCEEEEEC
>Mature Secondary Structure
MEQARALLQGKTVTIMGLGVHGGGCAAACFCAEAGARLTVTDLRNADALTPSLKRLRAYP
CCHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHCCC
SIRFTLGEHRLEDFENAHVVIKNPIVKGAHNIYLSAAQRAGARIETDISLFLRLSPAPLL
CEEEEECHHHHHCCCCCEEEEECCCCCCCHHEEEEEHHHCCCEEEECEEEEEEECCCCEE
AVSGSKGKSSTASALCYSLRALGFPAFLGGNSTVSPLEFVRHTTPATPVVLELSSWQLAD
EEECCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHCCCCCCEEEEECCCCEEE
LRAVDAQDHTVHHAGLLRPEIAIMTPIMADHQNWYADMESYVADKQVLYAHQGTHDTLLC
EEEECCCCCEEHHHCCCCCCHHHCCCHHHCCHHHHHHHHHHHCCCEEEEEECCCCCEEEE
NADDGWGPRFACEAQKNGVRVFWYTAQSPETACRACTPRLMERALWRATDGTYWARFAEG
ECCCCCCCCEEEECCCCCEEEEEEECCCCHHHHHHCCHHHHHHHHHHCCCCCEEEEECCC
DRACMLIPPQLHVPGRVLQTQVASAALAALLFAQRHSLPPSSCPPCFCAHSHSPAYANHA
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCC
SPPDYACPSAHSPFQEHTRRLAQALESYTGIEHRLEFFYEKGGLRFYNDSASTVPEATIA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEECCCCCCCCHHHHH
ALEAFDESVVLIVGGTDKNADYQPLAQAAAKAHALYLLAGSATARLQPLLHAAQVPFYGP
HHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHCCCCCCC
FTSLEVLLQDLRARQKSPGVIVFSPGAASFELFAHEFERGTTFKSQVRIIFE
HHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876