Click here to switch to the map view.

The map label for this gene is nifS2 [C]

Identifier: 189026087

GI number: 189026087

Start: 941795

End: 943078

Strand: Direct

Name: nifS2 [C]

Synonym: TPASS_0863

Alternate gene names: 189026087

Gene position: 941795-943078 (Clockwise)

Preceding gene: 189026084

Following gene: 189026091

Centisome position: 82.65

GC content: 57.94

Gene sequence:

>1284_bases
ATGAACCTCTATTTGGATTGGGCTGCAGCTGCTATACCCGAAACCCGGCTTATCCTACGCGAAACGCAACAGGCCTGTAC
TCTCTTTGCCAACCCTTCTGCTGTGCATTGCTTGGGAAACGATGCGCGCGATGCGCTCGAACGAGCACGACACACGTGTG
CCCAGAGCTTAGGCATCAATCCAGAACGCCTTATATTCACTTCAGGCGGGAGCGAAGCAAATCAACTTGCCCTCCTTTCT
GTCCTTACTCGTCTTCCTCACGCAGAAATCAGCGTCAGTATGCTAGAGCACGCGTCGGTCACTGCACTTTTGCCCCGGCT
TGAGCGGTTGCAGGTATCCGTACGCCACATCCCCGTCAATGCCCGCGGTTTCATTACCCCTGAGGCTGTACGTGCAACGC
TCAGTCCCCGTACCACGCTAGTGTGCGTGAGCGCCGTACATAGTGAAACCGGCGCCATCCAGCCGCTCCCTGCTATTGCG
CACGTGCTTGCACATACAGGCACACGCGGACGCTCTATCCAGCTCCACGTAGACGCCGCACAGGCCTTTGGGAAAATACC
GCTCAATCTGTATATGGACCTTCCGCGCATAGAGGAACATGCACAGGAAAACAACGCGCCACAGACACCACCGGGCTACC
CCGCACCCACTGCACAACGCGCGCTTACCTACTCGGTAGCAATCAGTGGCCACAAAATAGGCGCACCACGGGGTATTGGG
CTACTGTGCGCACACCGTTCATTTACCCCCTTTGTCCTGGGAGGCGGACAGGAAAAAGAGCGCCGCCCGGGAACTGAGAA
CCTTGCAGGTGCGCTCGCGCTCGCCGCTTGCGTGCGCGAAGGCGCCTTCTTCCGTACTCTACATACCACTCCGGAAGGCC
CTACACCGCATACGAAGCCCACAGCTCCTGCAGGGTTACGCAGTGTCCGAGCGCGTACGTGCGCCTTTGTGCGTGCACTC
AGCGATTTACCGCGGGTGCAACTAGTCCCTGCAACGCGCAAAGAAGACGAAGCGCACTTCTCTCCCTACATCGTCTGCTG
TGCGGTACAAGACGCCAGCGGCGAGGCATTAGTACGTGCATTCTCAGACGCAGGTGTGTGCATCTCCACCGGTTCTGCCT
GCTCGACAAAGAAAGGTGGCGTTTCAACACGCCTTCTGCGTGCACTCGGGGTAGAATCCCGCGCAACGCGCGGCGTGCTG
CGTTTCTCTTTTGGTCCACACACCACCGCCGAAGATCTCGATCGGGTCTTAACGCTTTTCCGTACCCTGCTGCAAAAACT
ATGA

Upstream 100 bases:

>100_bases
GGCGACCAGCCTACCCCATCCGTACTTCACACGTCAAGGAGATAGCTAATGCCCCGATCCTTCCTTGAGCCACACCCACT
TTTCAGGCATCGTACGCACC

Downstream 100 bases:

>100_bases
CCGCTCCACACAGAAAGGGTACACGCACAGGAAGCACTCAAGCGCCGCACACAACCCCTTATTTTGTCAAAATTTTTAAC
GGATTTACCACCGACCCGTT

Product: nitrogen fixation protein

Products: L-alanine; S [C]

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MNLYLDWAAAAIPETRLILRETQQACTLFANPSAVHCLGNDARDALERARHTCAQSLGINPERLIFTSGGSEANQLALLS
VLTRLPHAEISVSMLEHASVTALLPRLERLQVSVRHIPVNARGFITPEAVRATLSPRTTLVCVSAVHSETGAIQPLPAIA
HVLAHTGTRGRSIQLHVDAAQAFGKIPLNLYMDLPRIEEHAQENNAPQTPPGYPAPTAQRALTYSVAISGHKIGAPRGIG
LLCAHRSFTPFVLGGGQEKERRPGTENLAGALALAACVREGAFFRTLHTTPEGPTPHTKPTAPAGLRSVRARTCAFVRAL
SDLPRVQLVPATRKEDEAHFSPYIVCCAVQDASGEALVRAFSDAGVCISTGSACSTKKGGVSTRLLRALGVESRATRGVL
RFSFGPHTTAEDLDRVLTLFRTLLQKL

Sequences:

>Translated_427_residues
MNLYLDWAAAAIPETRLILRETQQACTLFANPSAVHCLGNDARDALERARHTCAQSLGINPERLIFTSGGSEANQLALLS
VLTRLPHAEISVSMLEHASVTALLPRLERLQVSVRHIPVNARGFITPEAVRATLSPRTTLVCVSAVHSETGAIQPLPAIA
HVLAHTGTRGRSIQLHVDAAQAFGKIPLNLYMDLPRIEEHAQENNAPQTPPGYPAPTAQRALTYSVAISGHKIGAPRGIG
LLCAHRSFTPFVLGGGQEKERRPGTENLAGALALAACVREGAFFRTLHTTPEGPTPHTKPTAPAGLRSVRARTCAFVRAL
SDLPRVQLVPATRKEDEAHFSPYIVCCAVQDASGEALVRAFSDAGVCISTGSACSTKKGGVSTRLLRALGVESRATRGVL
RFSFGPHTTAEDLDRVLTLFRTLLQKL
>Mature_427_residues
MNLYLDWAAAAIPETRLILRETQQACTLFANPSAVHCLGNDARDALERARHTCAQSLGINPERLIFTSGGSEANQLALLS
VLTRLPHAEISVSMLEHASVTALLPRLERLQVSVRHIPVNARGFITPEAVRATLSPRTTLVCVSAVHSETGAIQPLPAIA
HVLAHTGTRGRSIQLHVDAAQAFGKIPLNLYMDLPRIEEHAQENNAPQTPPGYPAPTAQRALTYSVAISGHKIGAPRGIG
LLCAHRSFTPFVLGGGQEKERRPGTENLAGALALAACVREGAFFRTLHTTPEGPTPHTKPTAPAGLRSVRARTCAFVRAL
SDLPRVQLVPATRKEDEAHFSPYIVCCAVQDASGEALVRAFSDAGVCISTGSACSTKKGGVSTRLLRALGVESRATRGVL
RFSFGPHTTAEDLDRVLTLFRTLLQKL

Specific function: Fe-S cluster biosynthesis [C]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=423, Percent_Identity=27.6595744680851, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI156713448, Length=460, Percent_Identity=26.5217391304348, Blast_Score=102, Evalue=9e-22,
Organism=Escherichia coli, GI48994898, Length=438, Percent_Identity=28.0821917808219, Blast_Score=136, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI25143064, Length=429, Percent_Identity=26.5734265734266, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17533177, Length=242, Percent_Identity=28.9256198347107, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319831, Length=422, Percent_Identity=27.4881516587678, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI20129463, Length=429, Percent_Identity=28.6713286713287, Blast_Score=125, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: NA

Molecular weight: Translated: 45733; Mature: 45733

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLYLDWAAAAIPETRLILRETQQACTLFANPSAVHCLGNDARDALERARHTCAQSLGIN
CCEEEECHHHHCCHHHHHHHHHHHHHEEECCCCEEEECCCHHHHHHHHHHHHHHHHHCCC
PERLIFTSGGSEANQLALLSVLTRLPHAEISVSMLEHASVTALLPRLERLQVSVRHIPVN
CCEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCC
ARGFITPEAVRATLSPRTTLVCVSAVHSETGAIQPLPAIAHVLAHTGTRGRSIQLHVDAA
CCCCCCHHHHHHHCCCCEEEEEEEHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEHH
QAFGKIPLNLYMDLPRIEEHAQENNAPQTPPGYPAPTAQRALTYSVAISGHKIGAPRGIG
HHHCCCCCEEEECCHHHHHHHHHCCCCCCCCCCCCCCHHHEEEEEEEECCCCCCCCCCCE
LLCAHRSFTPFVLGGGQEKERRPGTENLAGALALAACVREGAFFRTLHTTPEGPTPHTKP
EEEECCCCCCEEECCCCCHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCC
TAPAGLRSVRARTCAFVRALSDLPRVQLVPATRKEDEAHFSPYIVCCAVQDASGEALVRA
CCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCEEEEEEEECCCCHHHHHH
FSDAGVCISTGSACSTKKGGVSTRLLRALGVESRATRGVLRFSFGPHTTAEDLDRVLTLF
HCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCHHCCCEEEEECCCCCCHHHHHHHHHHH
RTLLQKL
HHHHHHC
>Mature Secondary Structure
MNLYLDWAAAAIPETRLILRETQQACTLFANPSAVHCLGNDARDALERARHTCAQSLGIN
CCEEEECHHHHCCHHHHHHHHHHHHHEEECCCCEEEECCCHHHHHHHHHHHHHHHHHCCC
PERLIFTSGGSEANQLALLSVLTRLPHAEISVSMLEHASVTALLPRLERLQVSVRHIPVN
CCEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCC
ARGFITPEAVRATLSPRTTLVCVSAVHSETGAIQPLPAIAHVLAHTGTRGRSIQLHVDAA
CCCCCCHHHHHHHCCCCEEEEEEEHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEHH
QAFGKIPLNLYMDLPRIEEHAQENNAPQTPPGYPAPTAQRALTYSVAISGHKIGAPRGIG
HHHCCCCCEEEECCHHHHHHHHHCCCCCCCCCCCCCCHHHEEEEEEEECCCCCCCCCCCE
LLCAHRSFTPFVLGGGQEKERRPGTENLAGALALAACVREGAFFRTLHTTPEGPTPHTKP
EEEECCCCCCEEECCCCCHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCC
TAPAGLRSVRARTCAFVRALSDLPRVQLVPATRKEDEAHFSPYIVCCAVQDASGEALVRA
CCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCEEEEEEEECCCCHHHHHH
FSDAGVCISTGSACSTKKGGVSTRLLRALGVESRATRGVLRFSFGPHTTAEDLDRVLTLF
HCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCHHCCCEEEEECCCCCCHHHHHHHHHHH
RTLLQKL
HHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-cysteine [C]

Specific reaction: L-cysteine = L-alanine + S [C]

General reaction: transfers sulfane sulfur to ThiI during in vitro biosynthesis of 4-thiouridine in E. coli tRNA [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8031904 [H]