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The map label for this gene is truA

Identifier: 189026054

GI number: 189026054

Start: 898075

End: 898917

Strand: Direct

Name: truA

Synonym: TPASS_0830

Alternate gene names: 189026054

Gene position: 898075-898917 (Clockwise)

Preceding gene: 189026053

Following gene: 189026057

Centisome position: 78.82

GC content: 50.89

Gene sequence:

>843_bases
ATGAATGATGTGCGCAAAATTCTCTTGCGTATTTCGTACGATGGAACACGATTTTGCGGATGGCAAAAACAGGTCTCAGG
CTCACGGGAACGTGCTCCCTCTGTCCAAGGTGAGTTGGAAAAAGTTGCTGAGAAAATTCACCACCAAAAGATAGCAGTCA
TCGGTTCAGGGAGAACAGACTCTGGCGTACACGCAGTAGGACAGGCAGCACATTTTTGTACCCCCATGAGAAATATACTC
GCGTATCGCTTTATCCCTGCATTTAATTCGCTGCTCCCGCACTCCATTCGCATTACAGACGCACGCGAAGTCTCCTCTCA
ACTCCACGCACGCTTCTCTGCCGTCATGCGCACGTACCGTTACCACCTCCACTGCGCACCCGTCGCATACGCGCACGAAC
TGCCTTACTGCTGGCACATTGCGAGAATGCCCGATATACACTTGCTCAATCAATATGCTGCAACACTCAAGGGAGAACTA
GACTGCACAAGCTTTGCTGCTGCAGGAGATAAAAGTGCGAGTAAATCGCGTTATTTTTACGACACACACTTTTCTTTCAA
CCATCGCGTACTGACCTTCGAAATCTCTGCTAATGCCTTTCTCTGGAAAATGGTGCGCTCTCTTACAGGAACCCTACTAC
ACTGCGAAAAGAAGCGGTGCTCCGTGCGCGAATTCGTCCGCATTTTGCACGCGAAAGACAGGCGCTTGCAGGGCCCACCG
CACCGCCGCATGGGCTATTCCTATGGAACATCCGTTACCCCGAACACTTACTCCGTGCAGAATAGGAACACCCTCGCACG
TGAACTGGCATCCACAGGCAATGCAAGGTGGAAGACGTATTAA

Upstream 100 bases:

>100_bases
TAGGAAAGTCTTCAAAAGAGAAGCCAGGTCCATTGCTGGAACTCGCCCGACAATTGTACGAAAACCTGCTCGCAGAATTA
CACGAAGACAGTGAAACTAC

Downstream 100 bases:

>100_bases
GCATGCACGTTACATCTCTTCAAGAAAAGGAATCAGCACCAGACGCATAGCTGTTCTCAGCACTATGCGCACCGCACGCA
CAAGTTCAAGCCTTGCACAC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MNDVRKILLRISYDGTRFCGWQKQVSGSRERAPSVQGELEKVAEKIHHQKIAVIGSGRTDSGVHAVGQAAHFCTPMRNIL
AYRFIPAFNSLLPHSIRITDAREVSSQLHARFSAVMRTYRYHLHCAPVAYAHELPYCWHIARMPDIHLLNQYAATLKGEL
DCTSFAAAGDKSASKSRYFYDTHFSFNHRVLTFEISANAFLWKMVRSLTGTLLHCEKKRCSVREFVRILHAKDRRLQGPP
HRRMGYSYGTSVTPNTYSVQNRNTLARELASTGNARWKTY

Sequences:

>Translated_280_residues
MNDVRKILLRISYDGTRFCGWQKQVSGSRERAPSVQGELEKVAEKIHHQKIAVIGSGRTDSGVHAVGQAAHFCTPMRNIL
AYRFIPAFNSLLPHSIRITDAREVSSQLHARFSAVMRTYRYHLHCAPVAYAHELPYCWHIARMPDIHLLNQYAATLKGEL
DCTSFAAAGDKSASKSRYFYDTHFSFNHRVLTFEISANAFLWKMVRSLTGTLLHCEKKRCSVREFVRILHAKDRRLQGPP
HRRMGYSYGTSVTPNTYSVQNRNTLARELASTGNARWKTY
>Mature_280_residues
MNDVRKILLRISYDGTRFCGWQKQVSGSRERAPSVQGELEKVAEKIHHQKIAVIGSGRTDSGVHAVGQAAHFCTPMRNIL
AYRFIPAFNSLLPHSIRITDAREVSSQLHARFSAVMRTYRYHLHCAPVAYAHELPYCWHIARMPDIHLLNQYAATLKGEL
DCTSFAAAGDKSASKSRYFYDTHFSFNHRVLTFEISANAFLWKMVRSLTGTLLHCEKKRCSVREFVRILHAKDRRLQGPP
HRRMGYSYGTSVTPNTYSVQNRNTLARELASTGNARWKTY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=257, Percent_Identity=29.5719844357977, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1788657, Length=263, Percent_Identity=32.319391634981, Blast_Score=113, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_TREPA (O83802)

Other databases:

- EMBL:   AE000520
- PIR:   H71276
- RefSeq:   NP_219266.1
- ProteinModelPortal:   O83802
- SMR:   O83802
- IntAct:   O83802
- GeneID:   2610952
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0830
- NMPDR:   fig|243276.1.peg.825
- TIGR:   TP_0830
- HOGENOM:   HBG621020
- OMA:   NNYKLTI
- ProtClustDB:   PRK00021
- BioCyc:   TPAL243276:TP_0830-MONOMER
- BRENDA:   5.4.99.12
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 32002; Mature: 32002

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: NA

Important sites: ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDVRKILLRISYDGTRFCGWQKQVSGSRERAPSVQGELEKVAEKIHHQKIAVIGSGRTD
CCHHHEEHHHCCCCCCEECCCHHHHCCCHHCCCCHHHHHHHHHHHHHHCEEEEEECCCCC
SGVHAVGQAAHFCTPMRNILAYRFIPAFNSLLPHSIRITDAREVSSQLHARFSAVMRTYR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHH
YHLHCAPVAYAHELPYCWHIARMPDIHLLNQYAATLKGELDCTSFAAAGDKSASKSRYFY
HHEEECCHHHHHCCCCEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCEEE
DTHFSFNHRVLTFEISANAFLWKMVRSLTGTLLHCEKKRCSVREFVRILHAKDRRLQGPP
EEEEEECCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCC
HRRMGYSYGTSVTPNTYSVQNRNTLARELASTGNARWKTY
CHHCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNDVRKILLRISYDGTRFCGWQKQVSGSRERAPSVQGELEKVAEKIHHQKIAVIGSGRTD
CCHHHEEHHHCCCCCCEECCCHHHHCCCHHCCCCHHHHHHHHHHHHHHCEEEEEECCCCC
SGVHAVGQAAHFCTPMRNILAYRFIPAFNSLLPHSIRITDAREVSSQLHARFSAVMRTYR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHH
YHLHCAPVAYAHELPYCWHIARMPDIHLLNQYAATLKGELDCTSFAAAGDKSASKSRYFY
HHEEECCHHHHHCCCCEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCEEE
DTHFSFNHRVLTFEISANAFLWKMVRSLTGTLLHCEKKRCSVREFVRILHAKDRRLQGPP
EEEEEECCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCC
HRRMGYSYGTSVTPNTYSVQNRNTLARELASTGNARWKTY
CHHCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876