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The map label for this gene is cheB

Identifier: 189025857

GI number: 189025857

Start: 690371

End: 691582

Strand: Direct

Name: cheB

Synonym: TPASS_0631

Alternate gene names: 189025857

Gene position: 690371-691582 (Clockwise)

Preceding gene: 189025856

Following gene: 189025858

Centisome position: 60.59

GC content: 53.38

Gene sequence:

>1212_bases
ATGAAAAATACTAATGATATTGCTGTTTTAATCGTGGACGATTCTGCGCTCATGAGAAAGGTTATTGGAAAAGTCATTGA
AGGAGCGCCGGGTCTTAGCATAGCGGGCAAGGCCATGAATGGGCGCTTTGCGCTTGACATGCTAGAGCGTGTACAGCCAG
ATGTCATCTTGCTCGACCTGGAAATGCCGCACATGAACGGGCTTCAGTTTCTTGAACAGCGGAAACGTCTACGCATTGAT
ATTCCAGTAATCATTCTCTCTAGCATTGCAAAAGAAGGTGCACGGGTCACGATGCAATGTTTGGAGCTGGGGGCAAGTGA
CTTTGTCACCAAGCCTTTTGGATCTGAGTCTGCGCATTTGCGCACGGTTTCGCGCAAGATTGTCGATTACGTTACCGCGT
ATGGGAGACGCTACAAGTTGCTGCGCCGCACGCGCCGCTGTCTTGCCATGGACACACCTGTTGAGAGACCTGCCGGCGAG
GAGGACTTGAATTGTTTAGATACCCAAGAGCGAGCCTCTCTGCCGTCTGTATGTGCACGTGCGCCCGCGCGTGACCGTGC
ATCCTACACAATCACCCCCACAGAGGGAGCGCGCCAAACGCGCATCGTGCCACTGCGTGAAAGTGGGGCACTGCAGATCA
TTGCAATCGGCGTATCAACGGGAGGACCTAGCGCGCTGCGTCATATCTTTGCACAGCTCGATGCAGATTTGCCGCAGCCG
GTGGTAGTAGTGCAGCACATGCCTGCAGGTTTCACGCGTGAATTTGCGTACAGTCTTAACCAGGTATGTGCGCTGGAAGT
AAAAGAAGCGCAAGAAGGAGACTTGGTGCGGCGTGGGCGTGTTTTGATTGCCCCCGGGGATCGCCATCTGACGGTAGAGC
GGCGCTCCCTGGCAACTGTTGCGCACATCAACTCGGATGAACCGGAAAACGGTCACCGACCCAGCGTTGATGTGTTATTT
GAGTCAGTAGCTCGCCATTTTGAAAACCGCGCATTGGGAATTTTAATGACAGGAATGGGAAGGGACGGTGCCGCGCAGCT
TGCGCGTTTATACACAGAAGGCTCGCGTACAATTGCGCAGGACGCAGATTCGTGCATTGTATATGGAATGCCGCGTGTAG
CCTGCGAACTTGGAGCAGTTGGCGAACAGGTGAGTCTTGATGATATGGCAGCGACAATAAATCGCTACGGCAAGGTGTTT
GCCTCTTCCTGA

Upstream 100 bases:

>100_bases
TGCCTGTACCAGAAAAACGATGAAGGGTCAGCGCATGAATGCCACGCGCGGTAAGGCTGCACACCAGAGCGTATACAGGC
AGGGGGCCACAGACTACCGT

Downstream 100 bases:

>100_bases
GACGGACACGCACCCGTGATATAGAGGAGGGCTGCGCGGTCGGCGCACGTGTTCCTGCGCCTGCACTCTTGAATACTGGA
CGGACCTTGATTTACAGTCT

Product: protein-glutamate methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MKNTNDIAVLIVDDSALMRKVIGKVIEGAPGLSIAGKAMNGRFALDMLERVQPDVILLDLEMPHMNGLQFLEQRKRLRID
IPVIILSSIAKEGARVTMQCLELGASDFVTKPFGSESAHLRTVSRKIVDYVTAYGRRYKLLRRTRRCLAMDTPVERPAGE
EDLNCLDTQERASLPSVCARAPARDRASYTITPTEGARQTRIVPLRESGALQIIAIGVSTGGPSALRHIFAQLDADLPQP
VVVVQHMPAGFTREFAYSLNQVCALEVKEAQEGDLVRRGRVLIAPGDRHLTVERRSLATVAHINSDEPENGHRPSVDVLF
ESVARHFENRALGILMTGMGRDGAAQLARLYTEGSRTIAQDADSCIVYGMPRVACELGAVGEQVSLDDMAATINRYGKVF
ASS

Sequences:

>Translated_403_residues
MKNTNDIAVLIVDDSALMRKVIGKVIEGAPGLSIAGKAMNGRFALDMLERVQPDVILLDLEMPHMNGLQFLEQRKRLRID
IPVIILSSIAKEGARVTMQCLELGASDFVTKPFGSESAHLRTVSRKIVDYVTAYGRRYKLLRRTRRCLAMDTPVERPAGE
EDLNCLDTQERASLPSVCARAPARDRASYTITPTEGARQTRIVPLRESGALQIIAIGVSTGGPSALRHIFAQLDADLPQP
VVVVQHMPAGFTREFAYSLNQVCALEVKEAQEGDLVRRGRVLIAPGDRHLTVERRSLATVAHINSDEPENGHRPSVDVLF
ESVARHFENRALGILMTGMGRDGAAQLARLYTEGSRTIAQDADSCIVYGMPRVACELGAVGEQVSLDDMAATINRYGKVF
ASS
>Mature_403_residues
MKNTNDIAVLIVDDSALMRKVIGKVIEGAPGLSIAGKAMNGRFALDMLERVQPDVILLDLEMPHMNGLQFLEQRKRLRID
IPVIILSSIAKEGARVTMQCLELGASDFVTKPFGSESAHLRTVSRKIVDYVTAYGRRYKLLRRTRRCLAMDTPVERPAGE
EDLNCLDTQERASLPSVCARAPARDRASYTITPTEGARQTRIVPLRESGALQIIAIGVSTGGPSALRHIFAQLDADLPQP
VVVVQHMPAGFTREFAYSLNQVCALEVKEAQEGDLVRRGRVLIAPGDRHLTVERRSLATVAHINSDEPENGHRPSVDVLF
ESVARHFENRALGILMTGMGRDGAAQLARLYTEGSRTIAQDADSCIVYGMPRVACELGAVGEQVSLDDMAATINRYGKVF
ASS

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=388, Percent_Identity=36.5979381443299, Blast_Score=221, Evalue=9e-59,
Organism=Escherichia coli, GI1786599, Length=123, Percent_Identity=34.1463414634146, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788550, Length=112, Percent_Identity=32.1428571428571, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB_TREPA (O83639)

Other databases:

- EMBL:   AE000520
- PIR:   E71300
- RefSeq:   NP_219069.1
- ProteinModelPortal:   O83639
- SMR:   O83639
- IntAct:   O83639
- GeneID:   2611478
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0631
- NMPDR:   fig|243276.1.peg.628
- TIGR:   TP_0631
- HOGENOM:   HBG705324
- OMA:   FVTKPKL
- ProtClustDB:   CLSK218660
- BioCyc:   TPAL243276:TP_0631-MONOMER
- BRENDA:   3.1.1.61
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 44099; Mature: 44099

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 219-219 ACT_SITE 246-246 ACT_SITE 342-342

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNTNDIAVLIVDDSALMRKVIGKVIEGAPGLSIAGKAMNGRFALDMLERVQPDVILLDL
CCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCCEEEEEE
EMPHMNGLQFLEQRKRLRIDIPVIILSSIAKEGARVTMQCLELGASDFVTKPFGSESAHL
CCCCCCHHHHHHHHCCEEECCHHHHHHHHHHCCHHHHHHHHHCCCHHCCCCCCCCCCHHH
RTVSRKIVDYVTAYGRRYKLLRRTRRCLAMDTPVERPAGEEDLNCLDTQERASLPSVCAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHC
APARDRASYTITPTEGARQTRIVPLRESGALQIIAIGVSTGGPSALRHIFAQLDADLPQP
CCCCCCCCEEECCCCCCCCEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCC
VVVVQHMPAGFTREFAYSLNQVCALEVKEAQEGDLVRRGRVLIAPGDRHLTVERRSLATV
EEEEECCCCCHHHHHHHHHHHHEEEEECCCCCCCHHHCCEEEEECCCCEEEEEHHHHEEE
AHINSDEPENGHRPSVDVLFESVARHFENRALGILMTGMGRDGAAQLARLYTEGSRTIAQ
EECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHHH
DADSCIVYGMPRVACELGAVGEQVSLDDMAATINRYGKVFASS
CCCCEEEECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKNTNDIAVLIVDDSALMRKVIGKVIEGAPGLSIAGKAMNGRFALDMLERVQPDVILLDL
CCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCCEEEEEE
EMPHMNGLQFLEQRKRLRIDIPVIILSSIAKEGARVTMQCLELGASDFVTKPFGSESAHL
CCCCCCHHHHHHHHCCEEECCHHHHHHHHHHCCHHHHHHHHHCCCHHCCCCCCCCCCHHH
RTVSRKIVDYVTAYGRRYKLLRRTRRCLAMDTPVERPAGEEDLNCLDTQERASLPSVCAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHC
APARDRASYTITPTEGARQTRIVPLRESGALQIIAIGVSTGGPSALRHIFAQLDADLPQP
CCCCCCCCEEECCCCCCCCEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCC
VVVVQHMPAGFTREFAYSLNQVCALEVKEAQEGDLVRRGRVLIAPGDRHLTVERRSLATV
EEEEECCCCCHHHHHHHHHHHHEEEEECCCCCCCHHHCCEEEEECCCCEEEEEHHHHEEE
AHINSDEPENGHRPSVDVLFESVARHFENRALGILMTGMGRDGAAQLARLYTEGSRTIAQ
EECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHHH
DADSCIVYGMPRVACELGAVGEQVSLDDMAATINRYGKVFASS
CCCCEEEECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876