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The map label for this gene is sbcD [H]

Identifier: 189025852

GI number: 189025852

Start: 682455

End: 683630

Strand: Direct

Name: sbcD [H]

Synonym: TPASS_0626

Alternate gene names: 189025852

Gene position: 682455-683630 (Clockwise)

Preceding gene: 189025851

Following gene: 189025853

Centisome position: 59.89

GC content: 49.15

Gene sequence:

>1176_bases
ATGAAGATCCTGCACACAGCGGACCTACATCTAGGCAAAACACTCCATGAAGTATCGCTTTTTGCGTCACAGAAAAAAAT
GCTCGGCGATCTGTGCACCCTCCTTGCGCAGGACAACTACGCCGCGCTCATCATCGCAGGCGACATCTATGACCGCTGTG
TACCCTCTGCAGAGAGTGTCAGTCTTTTTAGTTCTTTTTTGCAAAATATCAAACGGTCCATGCCACGGCTCCCGATATAT
CTCATCCCCGGCAACCATGATTCTGCGCAACGTCTCTCCTTTGCCCAGGAGCTACTTAAGCAGCAGGGAGTATTCATTGC
GCAGGATCCTGAAGAGAGCACCCGTCCCCATCTCCTCTGTCACGAGGGGGAAACAGTGCAGTTATTTTTACTTCCCTTTC
TCCACGCAGGTGCCTTTTCCTATCTTGATGAGGAAAACACCACTTGTCTCATTCACACCCAATCCGAACTCCTTCAAGAA
GCCTCGCGTCGCTTGCAGCGTGCAGTATCGTTGGACACCCCTTCTATCCTTGTCGCACACCTATTTACCCAAAAAGGTAT
TAGCTGCGAAAGTGAACGCCCGTTTGTTGGCAATGCCGTTTACGCTGACCCACACTGGTTTGACTTTTTCACCTATGTTG
CACTTGGTCATTTACACAAATGTCAAAAAATCACCGAACGCATGTACTATTCCGGATCTCCTTTGCCCTATTCGTTTGAC
GAAGCAAATACCCAAAAGGTTGCGCTTTCTGTAGAGATTCACTGCAACACAAAGGGATTCCCCATCCATGTGACTCCCCT
TCCACTTGAGCCACTTATCCCTCTTCGCACCATACGCGACTCATTCCACGCACTATATACCGGTGATCGCTATCTCCTTT
ATCAACGTGATTTTTTAGAAATCACCCTGACCGACCCGGCGCTCGTGCACAATCCTATTGGCCTTTTGAAGCCGCGCTAT
CCAGGATTGCTCAGTATCAAGCAGGAAAATGCGTTCGCCTTTGATATACCCCCCCCCTACTCCTCTAACGAGGGGATAGC
GCCCTGCACACACCACTCATTGCGCACACACTTTGATGTATTTATGCACGAAGTAAGCCCCACTCCTGATGACAGAGAAA
AGGGCGCTCTCTTTCAGGAACTTTTTGACGAAATGCAACAGGAATTCTCATCGTGA

Upstream 100 bases:

>100_bases
ACGCGCGCGATACCGCAAAAAAGAAGACAGACCAAGAAGAACACACCATGCGCAAAGCAAACGCGCCTAAAACCAAAGCG
TCTGGAGAAGCACCCACCCC

Downstream 100 bases:

>100_bases
AGCCGATGCGTCTTACGCTCCACAACATCGGTCCTTTCGTTGGCACCCATACAGTTGACTTCACCGCGCTCGGTCCTATT
TTTCTAGTGTGTGGGAAAAC

Product: exonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MKILHTADLHLGKTLHEVSLFASQKKMLGDLCTLLAQDNYAALIIAGDIYDRCVPSAESVSLFSSFLQNIKRSMPRLPIY
LIPGNHDSAQRLSFAQELLKQQGVFIAQDPEESTRPHLLCHEGETVQLFLLPFLHAGAFSYLDEENTTCLIHTQSELLQE
ASRRLQRAVSLDTPSILVAHLFTQKGISCESERPFVGNAVYADPHWFDFFTYVALGHLHKCQKITERMYYSGSPLPYSFD
EANTQKVALSVEIHCNTKGFPIHVTPLPLEPLIPLRTIRDSFHALYTGDRYLLYQRDFLEITLTDPALVHNPIGLLKPRY
PGLLSIKQENAFAFDIPPPYSSNEGIAPCTHHSLRTHFDVFMHEVSPTPDDREKGALFQELFDEMQQEFSS

Sequences:

>Translated_391_residues
MKILHTADLHLGKTLHEVSLFASQKKMLGDLCTLLAQDNYAALIIAGDIYDRCVPSAESVSLFSSFLQNIKRSMPRLPIY
LIPGNHDSAQRLSFAQELLKQQGVFIAQDPEESTRPHLLCHEGETVQLFLLPFLHAGAFSYLDEENTTCLIHTQSELLQE
ASRRLQRAVSLDTPSILVAHLFTQKGISCESERPFVGNAVYADPHWFDFFTYVALGHLHKCQKITERMYYSGSPLPYSFD
EANTQKVALSVEIHCNTKGFPIHVTPLPLEPLIPLRTIRDSFHALYTGDRYLLYQRDFLEITLTDPALVHNPIGLLKPRY
PGLLSIKQENAFAFDIPPPYSSNEGIAPCTHHSLRTHFDVFMHEVSPTPDDREKGALFQELFDEMQQEFSS
>Mature_391_residues
MKILHTADLHLGKTLHEVSLFASQKKMLGDLCTLLAQDNYAALIIAGDIYDRCVPSAESVSLFSSFLQNIKRSMPRLPIY
LIPGNHDSAQRLSFAQELLKQQGVFIAQDPEESTRPHLLCHEGETVQLFLLPFLHAGAFSYLDEENTTCLIHTQSELLQE
ASRRLQRAVSLDTPSILVAHLFTQKGISCESERPFVGNAVYADPHWFDFFTYVALGHLHKCQKITERMYYSGSPLPYSFD
EANTQKVALSVEIHCNTKGFPIHVTPLPLEPLIPLRTIRDSFHALYTGDRYLLYQRDFLEITLTDPALVHNPIGLLKPRY
PGLLSIKQENAFAFDIPPPYSSNEGIAPCTHHSLRTHFDVFMHEVSPTPDDREKGALFQELFDEMQQEFSS

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=257, Percent_Identity=26.8482490272374, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 44298; Mature: 44298

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILHTADLHLGKTLHEVSLFASQKKMLGDLCTLLAQDNYAALIIAGDIYDRCVPSAESV
CCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHCCCHHHH
SLFSSFLQNIKRSMPRLPIYLIPGNHDSAQRLSFAQELLKQQGVFIAQDPEESTRPHLLC
HHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEE
HEGETVQLFLLPFLHAGAFSYLDEENTTCLIHTQSELLQEASRRLQRAVSLDTPSILVAH
ECCCEEEEEEHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHH
LFTQKGISCESERPFVGNAVYADPHWFDFFTYVALGHLHKCQKITERMYYSGSPLPYSFD
HHHHCCCCCCCCCCEECCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
EANTQKVALSVEIHCNTKGFPIHVTPLPLEPLIPLRTIRDSFHALYTGDRYLLYQRDFLE
CCCCEEEEEEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHEEEECCEEEEEECCEEE
ITLTDPALVHNPIGLLKPRYPGLLSIKQENAFAFDIPPPYSSNEGIAPCTHHSLRTHFDV
EEECCCHHHHCCHHHCCCCCCCEEEEECCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHH
FMHEVSPTPDDREKGALFQELFDEMQQEFSS
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKILHTADLHLGKTLHEVSLFASQKKMLGDLCTLLAQDNYAALIIAGDIYDRCVPSAESV
CCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHCCCHHHH
SLFSSFLQNIKRSMPRLPIYLIPGNHDSAQRLSFAQELLKQQGVFIAQDPEESTRPHLLC
HHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEE
HEGETVQLFLLPFLHAGAFSYLDEENTTCLIHTQSELLQEASRRLQRAVSLDTPSILVAH
ECCCEEEEEEHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHH
LFTQKGISCESERPFVGNAVYADPHWFDFFTYVALGHLHKCQKITERMYYSGSPLPYSFD
HHHHCCCCCCCCCCEECCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
EANTQKVALSVEIHCNTKGFPIHVTPLPLEPLIPLRTIRDSFHALYTGDRYLLYQRDFLE
CCCCEEEEEEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHEEEECCEEEEEECCEEE
ITLTDPALVHNPIGLLKPRYPGLLSIKQENAFAFDIPPPYSSNEGIAPCTHHSLRTHFDV
EEECCCHHHHCCHHHCCCCCCCEEEEECCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHH
FMHEVSPTPDDREKGALFQELFDEMQQEFSS
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA