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The map label for this gene is prfB

Identifier: 189025803

GI number: 189025803

Start: 627252

End: 628358

Strand: Direct

Name: prfB

Synonym: TPASS_0576

Alternate gene names: 189025803

Gene position: 627252-628358 (Clockwise)

Preceding gene: 189025801

Following gene: 189025804

Centisome position: 55.05

GC content: 56.01

Gene sequence:

>1107_bases
TTGGTCCACAAATCACCGCCCTCGAGGCGCGCGTGCAGGAAGTATGGGGGAGTCTTTGACGTTGCCGCATACGAGGCGCG
CATAGCAACGCTTGAGGCTGCTGCAGCAGCGCCTGACTTTTGGAGCGAACGCGCGCGTGCCGAAGCGCTGTTAGCGGAAC
TGAAAACGCTACGCGCAACGCTTGAGCCGTGGCGTGCGCTGCGCCGTGAGAGCGCAGATCTGCGCGCGTTGTACGAGCTT
GCCCGCGAGGCGCAAGACGCATCGCTGGAGCCAGAACTTTCCTCCCTTTTTTCAGACATTTCTGCTCGTTTCGAAGAGGC
ATCGCTTACCCGTCTCCTGCACGAAGAGGTAGACCGCCTCGACGCGTTTGTTACCATCCACTCCGGCGCAGGAGGAGTGG
AGGCCTGCGACTGGGCACAGATGCTCATGCGCATGTACACGCGCTGGGCAGAGCGGCGCAGCTTTTGCGTACACATAGTT
GACTTACTTGAGTCAGAAGGGGGAGTAAAATCGGTGACGTTAAAAATTTGCGGGTCACACGCCTTTGGTTTTCTCAAGGG
AGAAACGGGGGTACACCGGCTCGTGCGCATCAGTCCGTTTGACTCTGCCGCGCGCAGACATACCTCTTTTACCTCCACCT
ACGTCTTCCCCGTATTAGACGATCACGTTGAGGTGCACATACGGAGCGAAGACATGCGGGTAGATACCTACCGCTCAGGG
GGAGCAGGCGGTCAACATGTCAATAAAACGGACTCTGCCGTGCGCATCACGCATCTGCCTACAGGGATAGTAGTCACCTG
CCAGAACGAGCGCAGCCAAATCAGCAACCGTGCAACGGCGCTGAGCTTGTTACGCGCCCGCCTGTACGCCTATGAACGGC
AAAAAAAACAGCAGGAACATCAACGGTTTGCTTCTGAAAAGAAGGATATTTCGTGGGGAAATCAGATTCGCTCGTACGTC
TTTCATCCCTACACCATGGTTAAAGATCACCGCAGCAAGTGCGAAACGGGGAATATTCACGCAGTCATGGACGGAGCGTT
AGAACCGTTCATCCGTTCCTACTTGGAGTTTCTGTGTACCAGTACCCAGTGTGTAGAACCACAGTGA

Upstream 100 bases:

>100_bases
AGACGAGGAAGAACCCTCACCCCCTCGATCCCCATAGCGGAGGAGGCCTCAGGCGTTTTCTCCATACACAGAAAGGAAAA
GGCAATGGAAATCGAAGAAT

Downstream 100 bases:

>100_bases
ACGGGAGTTACGCGCAATCATTTGCAGCACTGCTTTTCTTTCCCCAAATCGCGGTCGCGTTTAGTGCAACGGCACCGGCG
CCGTCCTTTGACTCTTTCCT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MVHKSPPSRRACRKYGGVFDVAAYEARIATLEAAAAAPDFWSERARAEALLAELKTLRATLEPWRALRRESADLRALYEL
AREAQDASLEPELSSLFSDISARFEEASLTRLLHEEVDRLDAFVTIHSGAGGVEACDWAQMLMRMYTRWAERRSFCVHIV
DLLESEGGVKSVTLKICGSHAFGFLKGETGVHRLVRISPFDSAARRHTSFTSTYVFPVLDDHVEVHIRSEDMRVDTYRSG
GAGGQHVNKTDSAVRITHLPTGIVVTCQNERSQISNRATALSLLRARLYAYERQKKQQEHQRFASEKKDISWGNQIRSYV
FHPYTMVKDHRSKCETGNIHAVMDGALEPFIRSYLEFLCTSTQCVEPQ

Sequences:

>Translated_368_residues
MVHKSPPSRRACRKYGGVFDVAAYEARIATLEAAAAAPDFWSERARAEALLAELKTLRATLEPWRALRRESADLRALYEL
AREAQDASLEPELSSLFSDISARFEEASLTRLLHEEVDRLDAFVTIHSGAGGVEACDWAQMLMRMYTRWAERRSFCVHIV
DLLESEGGVKSVTLKICGSHAFGFLKGETGVHRLVRISPFDSAARRHTSFTSTYVFPVLDDHVEVHIRSEDMRVDTYRSG
GAGGQHVNKTDSAVRITHLPTGIVVTCQNERSQISNRATALSLLRARLYAYERQKKQQEHQRFASEKKDISWGNQIRSYV
FHPYTMVKDHRSKCETGNIHAVMDGALEPFIRSYLEFLCTSTQCVEPQ
>Mature_368_residues
MVHKSPPSRRACRKYGGVFDVAAYEARIATLEAAAAAPDFWSERARAEALLAELKTLRATLEPWRALRRESADLRALYEL
AREAQDASLEPELSSLFSDISARFEEASLTRLLHEEVDRLDAFVTIHSGAGGVEACDWAQMLMRMYTRWAERRSFCVHIV
DLLESEGGVKSVTLKICGSHAFGFLKGETGVHRLVRISPFDSAARRHTSFTSTYVFPVLDDHVEVHIRSEDMRVDTYRSG
GAGGQHVNKTDSAVRITHLPTGIVVTCQNERSQISNRATALSLLRARLYAYERQKKQQEHQRFASEKKDISWGNQIRSYV
FHPYTMVKDHRSKCETGNIHAVMDGALEPFIRSYLEFLCTSTQCVEPQ

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=324, Percent_Identity=33.641975308642, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI34577120, Length=285, Percent_Identity=33.3333333333333, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI166795305, Length=223, Percent_Identity=33.6322869955157, Blast_Score=108, Evalue=8e-24,
Organism=Escherichia coli, GI2367172, Length=343, Percent_Identity=46.3556851311953, Blast_Score=321, Evalue=5e-89,
Organism=Escherichia coli, GI1787462, Length=332, Percent_Identity=38.855421686747, Blast_Score=191, Evalue=9e-50,
Organism=Caenorhabditis elegans, GI17542784, Length=329, Percent_Identity=30.3951367781155, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321295, Length=288, Percent_Identity=33.3333333333333, Blast_Score=140, Evalue=3e-34,
Organism=Drosophila melanogaster, GI19921226, Length=315, Percent_Identity=34.6031746031746, Blast_Score=153, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF2_TREPA (O83585)

Other databases:

- EMBL:   AE000520
- PIR:   F71306
- RefSeq:   NP_219014.1
- ProteinModelPortal:   O83585
- SMR:   O83585
- IntAct:   O83585
- GeneID:   2611749
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0576
- NMPDR:   fig|243276.1.peg.573
- TIGR:   TP_0576
- HOGENOM:   HBG629764
- OMA:   TEMLLRM
- ProtClustDB:   CLSK2300251
- BioCyc:   TPAL243276:TP_0576-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00094
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374
- PANTHER:   PTHR11075:SF6
- SMART:   SM00937
- TIGRFAMs:   TIGR00020

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 41597; Mature: 41597

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVHKSPPSRRACRKYGGVFDVAAYEARIATLEAAAAAPDFWSERARAEALLAELKTLRAT
CCCCCCCHHHHHHHHCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LEPWRALRRESADLRALYELAREAQDASLEPELSSLFSDISARFEEASLTRLLHEEVDRL
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAFVTIHSGAGGVEACDWAQMLMRMYTRWAERRSFCVHIVDLLESEGGVKSVTLKICGSH
HEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCC
AFGFLKGETGVHRLVRISPFDSAARRHTSFTSTYVFPVLDDHVEVHIRSEDMRVDTYRSG
CCEEEECCCCHHHEEEECCCHHHHHHHCCCHHEEEEEEECCCEEEEEECCCEEEECCCCC
GAGGQHVNKTDSAVRITHLPTGIVVTCQNERSQISNRATALSLLRARLYAYERQKKQQEH
CCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRFASEKKDISWGNQIRSYVFHPYTMVKDHRSKCETGNIHAVMDGALEPFIRSYLEFLCT
HHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHC
STQCVEPQ
CCCCCCCC
>Mature Secondary Structure
MVHKSPPSRRACRKYGGVFDVAAYEARIATLEAAAAAPDFWSERARAEALLAELKTLRAT
CCCCCCCHHHHHHHHCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LEPWRALRRESADLRALYELAREAQDASLEPELSSLFSDISARFEEASLTRLLHEEVDRL
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAFVTIHSGAGGVEACDWAQMLMRMYTRWAERRSFCVHIVDLLESEGGVKSVTLKICGSH
HEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCC
AFGFLKGETGVHRLVRISPFDSAARRHTSFTSTYVFPVLDDHVEVHIRSEDMRVDTYRSG
CCEEEECCCCHHHEEEECCCHHHHHHHCCCHHEEEEEEECCCEEEEEECCCEEEECCCCC
GAGGQHVNKTDSAVRITHLPTGIVVTCQNERSQISNRATALSLLRARLYAYERQKKQQEH
CCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRFASEKKDISWGNQIRSYVFHPYTMVKDHRSKCETGNIHAVMDGALEPFIRSYLEFLCT
HHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHC
STQCVEPQ
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876