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The map label for this gene is trmE

Identifier: 189025778

GI number: 189025778

Start: 597016

End: 598503

Strand: Reverse

Name: trmE

Synonym: TPASS_0550

Alternate gene names: 189025778

Gene position: 598503-597016 (Counterclockwise)

Preceding gene: 189025779

Following gene: 189025775

Centisome position: 52.53

GC content: 56.18

Gene sequence:

>1488_bases
ATGCGCGCACATGAGTATGCGCTCGACGATGACATCGTCGCCATTGCCACGGCGCTCAGTCCTGCCGCGTTAGGTATTGT
CCGCACGAGTGGGAGCAGCTCCATTGAGCGTGTTGCGTCCTTTTTTTCTCGTGCGCAGGCTCTGACGCGCGCACGTGCGC
ATACGTTTCTCCACGGATGGATTTTGGACGGAAAGACACGGGTGGATGAGGTAGTACTGCTGGTGTACCGCGCACCGCAC
AGTTTTACGGGAGAACATGCGGTAGAAATTATTTGTCATGGGGGCGTGCGTACCGTACAGGCTGTGTATCGGCTCTGTCT
TGCGCAGGGGTTCCGTGCCGCCCAGCGAGGTGAATTTAGTTTTCGTTCATTCTTTCATGGTAAAAGGGATTTGACGCGTA
TTGAGGCGATTCAGTCACTAGTTGATGCACGCACGTGTGCGGCGCAGCAGCAAGCGGTGTTGCACCTTTCAGGTGCGCTG
CAGCAGGAGATAGCAGCGCTTACCCGTGCGTTACTCGCGTTTTCAGCCACGCTTCAGGGGGAAATTGAGTATCCGGAAGA
TGAAGAAACGCGTGTGCACGATATCGATATGCGTGAACTTGAACCGTTGGTAGAACGTCTGCGCAGGCTCCGTGCCTGCT
GGCAGGAGCGTGCGCTGCAGCGAACGGGAGTGCGCATCGTGCTCGGCGGTTGTCCAAATGCGGGTAAATCATCTCTTTTC
AACGCTCTTTTAGGACAGGATCGTGCCATCGTTTCGTCCGTTCCGGGTACAACGCGCGATTGGCTTGAAGCAGACCTTGA
TCTATCGGGCATTCCAGTGCGTCTGTGTGATACGGCAGGCTTACGAGTAACTGACAATCCTATTGAGGCACAGGGAGTGG
TACGCAGCGAGCAACTCTTGCAGGGAGCAGATTGCGTTTTTTACATTATTAATGGACGTGCAGGTGTGCAGGCTGCAGAT
TGCGCTTTTCTTTCGGACTGTGCAGTACCCTTGGTAGTGGTAGTTACCCACAACGATTTGATGTCTATGTCTGAGCGCAT
ACAGGTGTGTCAGGCAGTGCAGCCGTTTATATCGGCACCGGTGTTGTCGTGTGCGCGCAGTCAGGACGCGCGCGGGGCGG
GGGAGCAGTGTTTAGCAGGGGGAAAGAACGGGGAGGTGAGGGATCGTGCGCCGCGCGCATTCGTGTGTGTAAGCGCAAAG
ACTCACGCGGGGTTGGACGCGCTCCGTGCACAGACACTCCACCTGCTGCACGGCGGTCAGGTGCCGTACGAGGAGCTTTC
TCTTGGCTCAGAACGCCAGTACGTATTGGTAGACGCCGCAGTGCAGGCGTTGGAGCATGCACAGGAGGCGTATGCACGTG
GGTTCGGGTTGGATGCAGTGGTGCACGATCTGGAAGAGGCGCTCTATCATTGTGGGGCGCTTACTGGAGAAGTGCACAGC
GAAGATATCCTAGATGCGCTGTTTGAGAAGCTCTGTGTGGGAAAGTGA

Upstream 100 bases:

>100_bases
GAGTACGGGAGTGGTGCCGCTGCTTTTTCTCCGTGTGGGACTTGCAGGCGGCTTGCCTTTTCACGCACCTGCGCCCACGG
CCGTGAGGGAACGGTAGCTG

Downstream 100 bases:

>100_bases
CCTGCAGGTACGGGAGATACGGGCGCGTGTGACTGATTAGTTATATTTCTTGGGGATGCAGCGTCAGGTAACTTTTTTCG
GTAGAAAGGTGGAGGCGCGC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MRAHEYALDDDIVAIATALSPAALGIVRTSGSSSIERVASFFSRAQALTRARAHTFLHGWILDGKTRVDEVVLLVYRAPH
SFTGEHAVEIICHGGVRTVQAVYRLCLAQGFRAAQRGEFSFRSFFHGKRDLTRIEAIQSLVDARTCAAQQQAVLHLSGAL
QQEIAALTRALLAFSATLQGEIEYPEDEETRVHDIDMRELEPLVERLRRLRACWQERALQRTGVRIVLGGCPNAGKSSLF
NALLGQDRAIVSSVPGTTRDWLEADLDLSGIPVRLCDTAGLRVTDNPIEAQGVVRSEQLLQGADCVFYIINGRAGVQAAD
CAFLSDCAVPLVVVVTHNDLMSMSERIQVCQAVQPFISAPVLSCARSQDARGAGEQCLAGGKNGEVRDRAPRAFVCVSAK
THAGLDALRAQTLHLLHGGQVPYEELSLGSERQYVLVDAAVQALEHAQEAYARGFGLDAVVHDLEEALYHCGALTGEVHS
EDILDALFEKLCVGK

Sequences:

>Translated_495_residues
MRAHEYALDDDIVAIATALSPAALGIVRTSGSSSIERVASFFSRAQALTRARAHTFLHGWILDGKTRVDEVVLLVYRAPH
SFTGEHAVEIICHGGVRTVQAVYRLCLAQGFRAAQRGEFSFRSFFHGKRDLTRIEAIQSLVDARTCAAQQQAVLHLSGAL
QQEIAALTRALLAFSATLQGEIEYPEDEETRVHDIDMRELEPLVERLRRLRACWQERALQRTGVRIVLGGCPNAGKSSLF
NALLGQDRAIVSSVPGTTRDWLEADLDLSGIPVRLCDTAGLRVTDNPIEAQGVVRSEQLLQGADCVFYIINGRAGVQAAD
CAFLSDCAVPLVVVVTHNDLMSMSERIQVCQAVQPFISAPVLSCARSQDARGAGEQCLAGGKNGEVRDRAPRAFVCVSAK
THAGLDALRAQTLHLLHGGQVPYEELSLGSERQYVLVDAAVQALEHAQEAYARGFGLDAVVHDLEEALYHCGALTGEVHS
EDILDALFEKLCVGK
>Mature_495_residues
MRAHEYALDDDIVAIATALSPAALGIVRTSGSSSIERVASFFSRAQALTRARAHTFLHGWILDGKTRVDEVVLLVYRAPH
SFTGEHAVEIICHGGVRTVQAVYRLCLAQGFRAAQRGEFSFRSFFHGKRDLTRIEAIQSLVDARTCAAQQQAVLHLSGAL
QQEIAALTRALLAFSATLQGEIEYPEDEETRVHDIDMRELEPLVERLRRLRACWQERALQRTGVRIVLGGCPNAGKSSLF
NALLGQDRAIVSSVPGTTRDWLEADLDLSGIPVRLCDTAGLRVTDNPIEAQGVVRSEQLLQGADCVFYIINGRAGVQAAD
CAFLSDCAVPLVVVVTHNDLMSMSERIQVCQAVQPFISAPVLSCARSQDARGAGEQCLAGGKNGEVRDRAPRAFVCVSAK
THAGLDALRAQTLHLLHGGQVPYEELSLGSERQYVLVDAAVQALEHAQEAYARGFGLDAVVHDLEEALYHCGALTGEVHS
EDILDALFEKLCVGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=504, Percent_Identity=30.952380952381, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI193082993, Length=493, Percent_Identity=31.237322515213, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI193082988, Length=536, Percent_Identity=29.1044776119403, Blast_Score=152, Evalue=5e-37,
Organism=Escherichia coli, GI2367268, Length=497, Percent_Identity=30.1810865191147, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI87082120, Length=141, Percent_Identity=32.6241134751773, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17507259, Length=490, Percent_Identity=29.1836734693878, Blast_Score=156, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6323665, Length=466, Percent_Identity=27.4678111587983, Blast_Score=150, Evalue=6e-37,
Organism=Drosophila melanogaster, GI45550813, Length=500, Percent_Identity=26.4, Blast_Score=148, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_TREPA (O83561)

Other databases:

- EMBL:   AE000520
- PIR:   B71311
- RefSeq:   NP_218989.1
- ProteinModelPortal:   O83561
- SMR:   O83561
- IntAct:   O83561
- GeneID:   2611188
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0550
- NMPDR:   fig|243276.1.peg.548
- TIGR:   TP_0550
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- BioCyc:   TPAL243276:TP_0550-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 53782; Mature: 53782

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00070 ALDEHYDE_DEHYDR_CYS

Important sites: BINDING 28-28 BINDING 89-89 BINDING 128-128 BINDING 495-495

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAHEYALDDDIVAIATALSPAALGIVRTSGSSSIERVASFFSRAQALTRARAHTFLHGW
CCCCCCCCCCCHHHHHHHHCHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEE
ILDGKTRVDEVVLLVYRAPHSFTGEHAVEIICHGGVRTVQAVYRLCLAQGFRAAQRGEFS
EECCCCHHHHHEEEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCHHHHCCCCH
FRSFFHGKRDLTRIEAIQSLVDARTCAAQQQAVLHLSGALQQEIAALTRALLAFSATLQG
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EIEYPEDEETRVHDIDMRELEPLVERLRRLRACWQERALQRTGVRIVLGGCPNAGKSSLF
CCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHH
NALLGQDRAIVSSVPGTTRDWLEADLDLSGIPVRLCDTAGLRVTDNPIEAQGVVRSEQLL
HHHHCCCCHHHHCCCCCCHHHHHHCCCCCCCCEEEECCCCCEEECCCCCHHHHHHHHHHH
QGADCVFYIINGRAGVQAADCAFLSDCAVPLVVVVTHNDLMSMSERIQVCQAVQPFISAP
CCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
VLSCARSQDARGAGEQCLAGGKNGEVRDRAPRAFVCVSAKTHAGLDALRAQTLHLLHGGQ
HHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCEEEEEECCHHCCHHHHHHHHHHHHCCCC
VPYEELSLGSERQYVLVDAAVQALEHAQEAYARGFGLDAVVHDLEEALYHCGALTGEVHS
CCHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCH
EDILDALFEKLCVGK
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MRAHEYALDDDIVAIATALSPAALGIVRTSGSSSIERVASFFSRAQALTRARAHTFLHGW
CCCCCCCCCCCHHHHHHHHCHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEE
ILDGKTRVDEVVLLVYRAPHSFTGEHAVEIICHGGVRTVQAVYRLCLAQGFRAAQRGEFS
EECCCCHHHHHEEEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCHHHHCCCCH
FRSFFHGKRDLTRIEAIQSLVDARTCAAQQQAVLHLSGALQQEIAALTRALLAFSATLQG
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EIEYPEDEETRVHDIDMRELEPLVERLRRLRACWQERALQRTGVRIVLGGCPNAGKSSLF
CCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHH
NALLGQDRAIVSSVPGTTRDWLEADLDLSGIPVRLCDTAGLRVTDNPIEAQGVVRSEQLL
HHHHCCCCHHHHCCCCCCHHHHHHCCCCCCCCEEEECCCCCEEECCCCCHHHHHHHHHHH
QGADCVFYIINGRAGVQAADCAFLSDCAVPLVVVVTHNDLMSMSERIQVCQAVQPFISAP
CCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
VLSCARSQDARGAGEQCLAGGKNGEVRDRAPRAFVCVSAKTHAGLDALRAQTLHLLHGGQ
HHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCEEEEEECCHHCCHHHHHHHHHHHHCCCC
VPYEELSLGSERQYVLVDAAVQALEHAQEAYARGFGLDAVVHDLEEALYHCGALTGEVHS
CCHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCH
EDILDALFEKLCVGK
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876