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The map label for this gene is ruvC

Identifier: 189025747

GI number: 189025747

Start: 559336

End: 559926

Strand: Reverse

Name: ruvC

Synonym: TPASS_0517

Alternate gene names: 189025747

Gene position: 559926-559336 (Counterclockwise)

Preceding gene: 189025748

Following gene: 189025746

Centisome position: 49.14

GC content: 46.87

Gene sequence:

>591_bases
GTGCACGCACAGAACGTAGATATTGCGCCGGGCAGTACCTCGACCGTTTCCATCATAGTGGGTATTGACCCAGGACTTGA
ATCTACCGGATACGGCGTTATAGAAGCAGGGGGAGGCAGTCTGCGCTGTCTTACCTACGGGGTGATTGTTACCCAAAGCA
ATCAGCCATCTGCTGCACGACTCAGACACATCTTCGATACCCTGCAACAGGTAATCTCAATATATCAACCTCAGTATTGC
GCAGTGGAGACAATCTATTTCGCAAAGAATGTAACCAGTGCGTTGTGTGTTGCGCAAGCGCGTGGGGTTGTATTACTTGC
TATGGCACAACAGCACATTTCAGTAGCTGAATACGCACCGAATGCGATTAAAAAAGCAATAACTGGTATTGCCCAAGCAG
AAAAAAGACAGGTACAGCATTTGGTAAAAATTTTACTCAATCTTAAGGATATACCTCATCCTGATCACGCTGCTGATGCC
CTAGCGGTTGCTGTTACCCATGTACACTGTTGTATGTCTTCAAACTATGCGGTAGGTTCAACGCGCTCTAGGGGAGCGTA
CGTTACGCTGTACAAAAAAGGTAAGAGATGA

Upstream 100 bases:

>100_bases
GCGCACCTGTCGTGCGCGTCGAAGCACACCGGGGGAGATTTTTGCTTTTCCTTTCTCCCCTCGGACGTTACACCATTGAT
CATCACGAGCGGGGTATTGC

Downstream 100 bases:

>100_bases
AAAGCAAGAGTTCTTTGTTGAAAAGTGGGTTGCTGCTTTCTCTTTTAACACTTGTCTCTCGTGTATTGGGTTTAGCGCGA
GAAGTAGTGAAGTCTACGCT

Product: Holliday junction nuclease, subunit C

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MHAQNVDIAPGSTSTVSIIVGIDPGLESTGYGVIEAGGGSLRCLTYGVIVTQSNQPSAARLRHIFDTLQQVISIYQPQYC
AVETIYFAKNVTSALCVAQARGVVLLAMAQQHISVAEYAPNAIKKAITGIAQAEKRQVQHLVKILLNLKDIPHPDHAADA
LAVAVTHVHCCMSSNYAVGSTRSRGAYVTLYKKGKR

Sequences:

>Translated_196_residues
MHAQNVDIAPGSTSTVSIIVGIDPGLESTGYGVIEAGGGSLRCLTYGVIVTQSNQPSAARLRHIFDTLQQVISIYQPQYC
AVETIYFAKNVTSALCVAQARGVVLLAMAQQHISVAEYAPNAIKKAITGIAQAEKRQVQHLVKILLNLKDIPHPDHAADA
LAVAVTHVHCCMSSNYAVGSTRSRGAYVTLYKKGKR
>Mature_196_residues
MHAQNVDIAPGSTSTVSIIVGIDPGLESTGYGVIEAGGGSLRCLTYGVIVTQSNQPSAARLRHIFDTLQQVISIYQPQYC
AVETIYFAKNVTSALCVAQARGVVLLAMAQQHISVAEYAPNAIKKAITGIAQAEKRQVQHLVKILLNLKDIPHPDHAADA
LAVAVTHVHCCMSSNYAVGSTRSRGAYVTLYKKGKR

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=161, Percent_Identity=43.4782608695652, Blast_Score=114, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_TREPA (O83530)

Other databases:

- EMBL:   AE000520
- PIR:   D71315
- RefSeq:   NP_218958.1
- ProteinModelPortal:   O83530
- SMR:   O83530
- IntAct:   O83530
- GeneID:   2611279
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0517
- NMPDR:   fig|243276.1.peg.517
- TIGR:   TP_0517
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- ProtClustDB:   CLSK218739
- BioCyc:   TPAL243276:TP_0517-MONOMER
- BRENDA:   3.1.22.4
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 20942; Mature: 20942

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAQNVDIAPGSTSTVSIIVGIDPGLESTGYGVIEAGGGSLRCLTYGVIVTQSNQPSAAR
CCCCCCCCCCCCCCEEEEEEEECCCCCCCCCEEEECCCCEEEEEEEEEEEECCCCCHHHH
LRHIFDTLQQVISIYQPQYCAVETIYFAKNVTSALCVAQARGVVLLAMAQQHISVAEYAP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHCH
NAIKKAITGIAQAEKRQVQHLVKILLNLKDIPHPDHAADALAVAVTHVHCCMSSNYAVGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCC
TRSRGAYVTLYKKGKR
CCCCCCEEEEEECCCC
>Mature Secondary Structure
MHAQNVDIAPGSTSTVSIIVGIDPGLESTGYGVIEAGGGSLRCLTYGVIVTQSNQPSAAR
CCCCCCCCCCCCCCEEEEEEEECCCCCCCCCEEEECCCCEEEEEEEEEEEECCCCCHHHH
LRHIFDTLQQVISIYQPQYCAVETIYFAKNVTSALCVAQARGVVLLAMAQQHISVAEYAP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHCH
NAIKKAITGIAQAEKRQVQHLVKILLNLKDIPHPDHAADALAVAVTHVHCCMSSNYAVGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCC
TRSRGAYVTLYKKGKR
CCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876