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The map label for this gene is murF

Identifier: 189025619

GI number: 189025619

Start: 411305

End: 412684

Strand: Direct

Name: murF

Synonym: TPASS_0386

Alternate gene names: 189025619

Gene position: 411305-412684 (Clockwise)

Preceding gene: 189025618

Following gene: 189025620

Centisome position: 36.1

GC content: 52.03

Gene sequence:

>1380_bases
ATGCTTCTGAGTTTTGATGAGGTATGTGCGGCAGTGCAAGGGGCTCGCGTGTGCGATGCACGAGGGGCGCGCGGTTTTGA
CGGGGTTTCCTTTGACAGTCGTGCGGTTGTGCCGCGTGATTTGTTTATTCCGCTCCGTGGTGCGCACGTAGATGGACATA
CGTTTGTTGAAGAAGCGCTGCAGAAGGGCGCAGTTGCAACGCTTATAGATCAAAGGTACCCCCATGCTGGGGAATATGTT
GCTTGGTGCACTCGCTTTGGAGCTGCCTGTATTGCGGTGCACGACACGCTCAGGGCGCTGCAAGATCTTGCCTCCTTTTA
CTGCAAAAAGTTTCCTGCGCTTATTAGGATTGGCATTACCGGATCAAGCGGAAAGACAACCGTAAAAGAAATGGCTCGCG
CGGTGTTCAGTGAACGGTATCGGGTGGTTGCGACGCCAGGAAATTTAAATTCAGAGATAGGTTTACCGCAGTCGCTGTTT
TTTGTGCGTGCAGAACATGAGGTAGGAATCTTTGAGCTGGGGATGAATCGTCGGGGGGAGATGCGTACGCTCGCTCAGAT
ACTTGTTCCTCATTACGCGATCATTACCAACGTAGGTTGTGCGCACGTAGGCATTCTGGGCACGCAGCAGGCAATTGCGG
AAGAGAAAAAAGAGATTTTTTCTCAATTTACTGAACACAGCGTGGGTTTTGTCCCCGACGATGCATATCGTGTATTCCTG
TCTAATATCCCGTACGGTAGGGTTGTAGTTTACGATCAGGGGGGGCGTGGTCTAGCAACTGAGGTGATAGATGAGGGTTT
ACGTGGTTCTCGCGTGTTATATCAAGGACGATGGATTCGCGTGCCACTACCTGGGGTGCATAACGCAAAGAATGCGCTTG
CAGTGATTGCGCTTGCCGCACAGGTAGGACTTCCTGCTGAGGAAATTCAGCGGGGGATGGAGCGGGTAAAACCGCCCTTT
GGCCGCTCTCATGTGGTGTGTGCGTCGCTGACCTTTTTGCTCGATTGTTACAATGCGAATCCTGATTCAATGGCTGCGGC
GCTTCATTTGTGTGCGCATATTTCTGCAGTATCAAAAGTATATGTGCTCGGTGACATGGGAGAGCTAGGAGTTACCGCTG
CCGAAGCTCACTATCGGGTGTGTGTTTTGGCAGCCGCTTCTGACGCGCGTGCAGTGTATGTGTTTGGTCCCGAATTTTGT
CGTGCGGTAAGAAAAGTTTCCTGGGGGAGGAAGCGCGTGTACGCCTTTGCACTAGAGGAACTGTCTGCGCTGCAAGAAAC
GTTAGACGCGCAATTACGGCGGGGTGATTTTGTTCTGGTGAAAGGTTCACGCAGTGTTGCGCTCGAGCGGCTTGAACCTC
TTTTGAGGAAAGAGCGATGA

Upstream 100 bases:

>100_bases
ATTGAAAAGCTCGCGGTAGAGAAACTGCACTTACACCGGGCGTCGCACGCCGAAATTGTGCGCATTAAGCTGCGTCCTGC
GCACAAGGACTCCTCCCCTC

Downstream 100 bases:

>100_bases
GACCGATGATTTCCATCGAGAAAAATGTACATCAAGAGCGTTACAGTTTTGTATTCATACTGCTTGTCGCCCTTATGGTA
GGAGTAGGTTTTGTAACGCT

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanine

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MLLSFDEVCAAVQGARVCDARGARGFDGVSFDSRAVVPRDLFIPLRGAHVDGHTFVEEALQKGAVATLIDQRYPHAGEYV
AWCTRFGAACIAVHDTLRALQDLASFYCKKFPALIRIGITGSSGKTTVKEMARAVFSERYRVVATPGNLNSEIGLPQSLF
FVRAEHEVGIFELGMNRRGEMRTLAQILVPHYAIITNVGCAHVGILGTQQAIAEEKKEIFSQFTEHSVGFVPDDAYRVFL
SNIPYGRVVVYDQGGRGLATEVIDEGLRGSRVLYQGRWIRVPLPGVHNAKNALAVIALAAQVGLPAEEIQRGMERVKPPF
GRSHVVCASLTFLLDCYNANPDSMAAALHLCAHISAVSKVYVLGDMGELGVTAAEAHYRVCVLAAASDARAVYVFGPEFC
RAVRKVSWGRKRVYAFALEELSALQETLDAQLRRGDFVLVKGSRSVALERLEPLLRKER

Sequences:

>Translated_459_residues
MLLSFDEVCAAVQGARVCDARGARGFDGVSFDSRAVVPRDLFIPLRGAHVDGHTFVEEALQKGAVATLIDQRYPHAGEYV
AWCTRFGAACIAVHDTLRALQDLASFYCKKFPALIRIGITGSSGKTTVKEMARAVFSERYRVVATPGNLNSEIGLPQSLF
FVRAEHEVGIFELGMNRRGEMRTLAQILVPHYAIITNVGCAHVGILGTQQAIAEEKKEIFSQFTEHSVGFVPDDAYRVFL
SNIPYGRVVVYDQGGRGLATEVIDEGLRGSRVLYQGRWIRVPLPGVHNAKNALAVIALAAQVGLPAEEIQRGMERVKPPF
GRSHVVCASLTFLLDCYNANPDSMAAALHLCAHISAVSKVYVLGDMGELGVTAAEAHYRVCVLAAASDARAVYVFGPEFC
RAVRKVSWGRKRVYAFALEELSALQETLDAQLRRGDFVLVKGSRSVALERLEPLLRKER
>Mature_459_residues
MLLSFDEVCAAVQGARVCDARGARGFDGVSFDSRAVVPRDLFIPLRGAHVDGHTFVEEALQKGAVATLIDQRYPHAGEYV
AWCTRFGAACIAVHDTLRALQDLASFYCKKFPALIRIGITGSSGKTTVKEMARAVFSERYRVVATPGNLNSEIGLPQSLF
FVRAEHEVGIFELGMNRRGEMRTLAQILVPHYAIITNVGCAHVGILGTQQAIAEEKKEIFSQFTEHSVGFVPDDAYRVFL
SNIPYGRVVVYDQGGRGLATEVIDEGLRGSRVLYQGRWIRVPLPGVHNAKNALAVIALAAQVGLPAEEIQRGMERVKPPF
GRSHVVCASLTFLLDCYNANPDSMAAALHLCAHISAVSKVYVLGDMGELGVTAAEAHYRVCVLAAASDARAVYVFGPEFC
RAVRKVSWGRKRVYAFALEELSALQETLDAQLRRGDFVLVKGSRSVALERLEPLLRKER

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786274, Length=465, Percent_Identity=30.1075268817204, Blast_Score=149, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURF_TREPA (O83401)

Other databases:

- EMBL:   AE000520
- PIR:   D71331
- RefSeq:   NP_218826.1
- ProteinModelPortal:   O83401
- SMR:   O83401
- IntAct:   O83401
- GeneID:   2611288
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0386
- NMPDR:   fig|243276.1.peg.385
- TIGR:   TP_0386
- HOGENOM:   HBG617121
- OMA:   ANPASMR
- ProtClustDB:   CLSK218674
- BioCyc:   TPAL243276:TP_0386-MONOMER
- BRENDA:   6.3.2.10
- GO:   GO:0005737
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- PANTHER:   PTHR23135:SF3
- TIGRFAMs:   TIGR01143

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.10

Molecular weight: Translated: 50236; Mature: 50236

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLSFDEVCAAVQGARVCDARGARGFDGVSFDSRAVVPRDLFIPLRGAHVDGHTFVEEAL
CCCCHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHH
QKGAVATLIDQRYPHAGEYVAWCTRFGAACIAVHDTLRALQDLASFYCKKFPALIRIGIT
HCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEE
GSSGKTTVKEMARAVFSERYRVVATPGNLNSEIGLPQSLFFVRAEHEVGIFELGMNRRGE
CCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCH
MRTLAQILVPHYAIITNVGCAHVGILGTQQAIAEEKKEIFSQFTEHSVGFVPDDAYRVFL
HHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
SNIPYGRVVVYDQGGRGLATEVIDEGLRGSRVLYQGRWIRVPLPGVHNAKNALAVIALAA
HCCCCCEEEEEECCCCCHHHHHHHCCCCCCEEEEECEEEEECCCCCCCCHHHHHHHHHHH
QVGLPAEEIQRGMERVKPPFGRSHVVCASLTFLLDCYNANPDSMAAALHLCAHISAVSKV
HHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEE
YVLGDMGELGVTAAEAHYRVCVLAAASDARAVYVFGPEFCRAVRKVSWGRKRVYAFALEE
EEEECCCCCCCEECCCCEEEEEEEECCCCCEEEEECHHHHHHHHHHCCCHHHHHHHHHHH
LSALQETLDAQLRRGDFVLVKGSRSVALERLEPLLRKER
HHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLLSFDEVCAAVQGARVCDARGARGFDGVSFDSRAVVPRDLFIPLRGAHVDGHTFVEEAL
CCCCHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHH
QKGAVATLIDQRYPHAGEYVAWCTRFGAACIAVHDTLRALQDLASFYCKKFPALIRIGIT
HCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEE
GSSGKTTVKEMARAVFSERYRVVATPGNLNSEIGLPQSLFFVRAEHEVGIFELGMNRRGE
CCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCH
MRTLAQILVPHYAIITNVGCAHVGILGTQQAIAEEKKEIFSQFTEHSVGFVPDDAYRVFL
HHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
SNIPYGRVVVYDQGGRGLATEVIDEGLRGSRVLYQGRWIRVPLPGVHNAKNALAVIALAA
HCCCCCEEEEEECCCCCHHHHHHHCCCCCCEEEEECEEEEECCCCCCCCHHHHHHHHHHH
QVGLPAEEIQRGMERVKPPFGRSHVVCASLTFLLDCYNANPDSMAAALHLCAHISAVSKV
HHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEE
YVLGDMGELGVTAAEAHYRVCVLAAASDARAVYVFGPEFCRAVRKVSWGRKRVYAFALEE
EEEECCCCCCCEECCCCEEEEEEEECCCCCEEEEECHHHHHHHHHHCCCHHHHHHHHHHH
LSALQETLDAQLRRGDFVLVKGSRSVALERLEPLLRKER
HHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876