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The map label for this gene is glyA

Identifier: 189025562

GI number: 189025562

Start: 351468

End: 353192

Strand: Direct

Name: glyA

Synonym: TPASS_0329

Alternate gene names: 189025562

Gene position: 351468-353192 (Clockwise)

Preceding gene: 189025561

Following gene: 189025565

Centisome position: 30.85

GC content: 54.38

Gene sequence:

>1725_bases
GTGAATAATTCCGCACTCCGTGCGTACCTGAGTACGCGTGCTCCTGACCAGATACATAGTGCTTTTGTTGCGTATTTGGC
CAATCTTGATTTAGTTGCGCACCAGTTTCCGCAGATTGCTTCTGATATTGTGCAGGAGCTGATAGATCAGCGGTCGTATG
TAAAGTTAATCGCAAGTGAGAATTACAGCTCTCTTGCGGTGCAAGCGGCGATGGCTAACTTGTTGACTGATAAATACGCA
GAAGGGTTCCCCCATCATCGCTACTATGGCGGGTGTCAGAATGTTGATTCTATTGAGTCTGCCGCCGCTGCAGAAGCATG
CGCGCTCTTTGGTGCTGAGCACGCATATGTCCAGCCGCACTCCGGTGCAGATGCGAATCTTGTTGCATTCTGGGCTATCC
TTTCGCGGCAAATTGAAATGCCAACCCTTTCTTCTCTTGGTGTCACCGCCGCTACGCATCTGAGTGAGGAACAGTGGGAA
GTACTGCGCCAGAAAATGGGTAATCAAAAACTTATGGGGTTAGATTATTTTTCAGGCGGTCACCTGACCCACGGGTACCG
CCAAAATGTTTCAGGACGAATGTTTCGTGTGGTGTCCTACGCGGTGGACCGAGACACAGGACTGCTCGATTACGCTGCAA
TCGAGGCACAGGCAAAGCGGGAAAGACCACTTATTTTACTTGCCGGATACAGCGCGTATCCTCGTTCCATTAATTTCCGC
ATCTTTCGGGAAATTGCAGACAAAGTGGGCGCAGTACTCATGGCTGATATGGCTCACTTTGCTGGACTGGTTGCAGGCGG
TGTTTTTACGGGAGACGAGGATCCAGTGCGCTGGTCTCATATCGTGACCAGTACCACACACAAAACGTTGCGCGGGCCAC
GCGGTGCCTTTATTTTGTGTAAAAAAGAATTTGCAGAGGCGGTGGATAAGGGCTGTCCGCTTGTGCTCGGCGGCCCGCTG
CCACATGTGATGGCAGCAAAGGCGGTTGCGTTTCGTGAAGCTCGAAATGCTGCTTTTAAAACCTATGCGCACGCAGTCCG
TGATAATGCGCGTGCGCTGGCAGATGCCTGCATACAACAGGGGATGCAGCTGCAGACAGGGGGGACGGATAACCATCTGC
TATTGCTTGACGTGCGTCCGTTTGGACTGACAGGTCGTCAGGCAGAGCGCGCGCTGATAGACTGCGGAGTGACGCTCAAC
CGTAACTCGCTCCCCTTTGACCCAAACGGCGCATGGCTCACCAGCGGACTGCGCATCGGAACCCCCGCGGTAACGAGCCT
TGGAATGGGGCCTGAGGAAATGAAAAGAATAGCGCGCCTGATCGCGCGCGTGCTCGGCGCTGCAACGCCTGTGCGGACAA
AGACAGGTGCGCTAAGCAAATCGGCGGCCGAGGTGCCCGGCGAGGTTAGAAGCTCAGTCTGCTCGGAAGTGCGGGAGCTG
CTCGCACGCTTCACGTTGTACCCTGAACTCGACGAACCCTTCTTGCGCGCACACTTTACGCGTCGCCCTGCGGGACAAAA
CACCTGCCGACGAAGGGACTTGAACCCTTACGGGGTTACCCCAACAGATTTTGAGTCTGTCGTGTCTGCCAGTTTCACCA
CGTCGGCCCGCGCGCAGCCTATCACACGAGGAACAAAAGGTACAGCTGTTCATGTAGTCTTCTTGCGTGAGGCCCCGTGT
CTCCCATTGAGGGAGCCGTTATTTTTCTCCCATGAGGAGTTTTAG

Upstream 100 bases:

>100_bases
ACGCACGACGGGTATCGGGCATGCGGCGCGCGGCGTTTTGTCCATTCTTTGCGCTAGAAACAGAGCGAACAATATTCTGC
CTACCTGAGGAGAGAAAAAC

Downstream 100 bases:

>100_bases
TTCCCGAATATCTGCCACCAGTTTAGAGCGATCTAAATGCTGATAACGCGCAGGGAGCATTTCCTTTCCTGTGCATTCGA
GTACTGCCACTAAATTTTGC

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MNNSALRAYLSTRAPDQIHSAFVAYLANLDLVAHQFPQIASDIVQELIDQRSYVKLIASENYSSLAVQAAMANLLTDKYA
EGFPHHRYYGGCQNVDSIESAAAAEACALFGAEHAYVQPHSGADANLVAFWAILSRQIEMPTLSSLGVTAATHLSEEQWE
VLRQKMGNQKLMGLDYFSGGHLTHGYRQNVSGRMFRVVSYAVDRDTGLLDYAAIEAQAKRERPLILLAGYSAYPRSINFR
IFREIADKVGAVLMADMAHFAGLVAGGVFTGDEDPVRWSHIVTSTTHKTLRGPRGAFILCKKEFAEAVDKGCPLVLGGPL
PHVMAAKAVAFREARNAAFKTYAHAVRDNARALADACIQQGMQLQTGGTDNHLLLLDVRPFGLTGRQAERALIDCGVTLN
RNSLPFDPNGAWLTSGLRIGTPAVTSLGMGPEEMKRIARLIARVLGAATPVRTKTGALSKSAAEVPGEVRSSVCSEVREL
LARFTLYPELDEPFLRAHFTRRPAGQNTCRRRDLNPYGVTPTDFESVVSASFTTSARAQPITRGTKGTAVHVVFLREAPC
LPLREPLFFSHEEF

Sequences:

>Translated_574_residues
MNNSALRAYLSTRAPDQIHSAFVAYLANLDLVAHQFPQIASDIVQELIDQRSYVKLIASENYSSLAVQAAMANLLTDKYA
EGFPHHRYYGGCQNVDSIESAAAAEACALFGAEHAYVQPHSGADANLVAFWAILSRQIEMPTLSSLGVTAATHLSEEQWE
VLRQKMGNQKLMGLDYFSGGHLTHGYRQNVSGRMFRVVSYAVDRDTGLLDYAAIEAQAKRERPLILLAGYSAYPRSINFR
IFREIADKVGAVLMADMAHFAGLVAGGVFTGDEDPVRWSHIVTSTTHKTLRGPRGAFILCKKEFAEAVDKGCPLVLGGPL
PHVMAAKAVAFREARNAAFKTYAHAVRDNARALADACIQQGMQLQTGGTDNHLLLLDVRPFGLTGRQAERALIDCGVTLN
RNSLPFDPNGAWLTSGLRIGTPAVTSLGMGPEEMKRIARLIARVLGAATPVRTKTGALSKSAAEVPGEVRSSVCSEVREL
LARFTLYPELDEPFLRAHFTRRPAGQNTCRRRDLNPYGVTPTDFESVVSASFTTSARAQPITRGTKGTAVHVVFLREAPC
LPLREPLFFSHEEF
>Mature_574_residues
MNNSALRAYLSTRAPDQIHSAFVAYLANLDLVAHQFPQIASDIVQELIDQRSYVKLIASENYSSLAVQAAMANLLTDKYA
EGFPHHRYYGGCQNVDSIESAAAAEACALFGAEHAYVQPHSGADANLVAFWAILSRQIEMPTLSSLGVTAATHLSEEQWE
VLRQKMGNQKLMGLDYFSGGHLTHGYRQNVSGRMFRVVSYAVDRDTGLLDYAAIEAQAKRERPLILLAGYSAYPRSINFR
IFREIADKVGAVLMADMAHFAGLVAGGVFTGDEDPVRWSHIVTSTTHKTLRGPRGAFILCKKEFAEAVDKGCPLVLGGPL
PHVMAAKAVAFREARNAAFKTYAHAVRDNARALADACIQQGMQLQTGGTDNHLLLLDVRPFGLTGRQAERALIDCGVTLN
RNSLPFDPNGAWLTSGLRIGTPAVTSLGMGPEEMKRIARLIARVLGAATPVRTKTGALSKSAAEVPGEVRSSVCSEVREL
LARFTLYPELDEPFLRAHFTRRPAGQNTCRRRDLNPYGVTPTDFESVVSASFTTSARAQPITRGTKGTAVHVVFLREAPC
LPLREPLFFSHEEF

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=480, Percent_Identity=36.0416666666667, Blast_Score=257, Evalue=2e-68,
Organism=Homo sapiens, GI261862350, Length=480, Percent_Identity=36.0416666666667, Blast_Score=257, Evalue=2e-68,
Organism=Homo sapiens, GI261862348, Length=480, Percent_Identity=36.0416666666667, Blast_Score=257, Evalue=2e-68,
Organism=Homo sapiens, GI19923315, Length=480, Percent_Identity=36.0416666666667, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI261862346, Length=480, Percent_Identity=35, Blast_Score=239, Evalue=7e-63,
Organism=Homo sapiens, GI22547186, Length=481, Percent_Identity=34.0956340956341, Blast_Score=238, Evalue=1e-62,
Organism=Homo sapiens, GI22547189, Length=464, Percent_Identity=33.6206896551724, Blast_Score=226, Evalue=4e-59,
Organism=Escherichia coli, GI1788902, Length=405, Percent_Identity=42.962962962963, Blast_Score=301, Evalue=5e-83,
Organism=Caenorhabditis elegans, GI25144732, Length=437, Percent_Identity=36.6132723112128, Blast_Score=264, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI25144729, Length=437, Percent_Identity=36.6132723112128, Blast_Score=264, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6319739, Length=472, Percent_Identity=36.4406779661017, Blast_Score=266, Evalue=5e-72,
Organism=Saccharomyces cerevisiae, GI6323087, Length=462, Percent_Identity=34.8484848484849, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24640005, Length=483, Percent_Identity=35.6107660455487, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI221329721, Length=483, Percent_Identity=35.6107660455487, Blast_Score=279, Evalue=4e-75,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_TREPA (O83349)

Other databases:

- EMBL:   AE000520
- PIR:   G71336
- RefSeq:   NP_218769.1
- ProteinModelPortal:   O83349
- SMR:   O83349
- IntAct:   O83349
- GeneID:   2611541
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0329
- NMPDR:   fig|243276.1.peg.328
- TIGR:   TP_0329
- HOGENOM:   HBG301263
- OMA:   IMTASTH
- ProtClustDB:   PRK13580
- BioCyc:   TPAL243276:TP_0329-MONOMER
- BRENDA:   2.1.2.1
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 62689; Mature: 62689

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNSALRAYLSTRAPDQIHSAFVAYLANLDLVAHQFPQIASDIVQELIDQRSYVKLIASE
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEHHHCC
NYSSLAVQAAMANLLTDKYAEGFPHHRYYGGCQNVDSIESAAAAEACALFGAEHAYVQPH
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCC
SGADANLVAFWAILSRQIEMPTLSSLGVTAATHLSEEQWEVLRQKMGNQKLMGLDYFSGG
CCCCCHHHHHHHHHHHHCCCCCCHHCCCHHHHHCCHHHHHHHHHHCCCCEEEEEEECCCC
HLTHGYRQNVSGRMFRVVSYAVDRDTGLLDYAAIEAQAKRERPLILLAGYSAYPRSINFR
CCCCHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHH
IFREIADKVGAVLMADMAHFAGLVAGGVFTGDEDPVRWSHIVTSTTHKTLRGPRGAFILC
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEE
KKEFAEAVDKGCPLVLGGPLPHVMAAKAVAFREARNAAFKTYAHAVRDNARALADACIQQ
HHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GMQLQTGGTDNHLLLLDVRPFGLTGRQAERALIDCGVTLNRNSLPFDPNGAWLTSGLRIG
CCEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCEECCCCCCCCCCCCEEECCCEEC
TPAVTSLGMGPEEMKRIARLIARVLGAATPVRTKTGALSKSAAEVPGEVRSSVCSEVREL
CCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHH
LARFTLYPELDEPFLRAHFTRRPAGQNTCRRRDLNPYGVTPTDFESVVSASFTTSARAQP
HHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
ITRGTKGTAVHVVFLREAPCLPLREPLFFSHEEF
CCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNNSALRAYLSTRAPDQIHSAFVAYLANLDLVAHQFPQIASDIVQELIDQRSYVKLIASE
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEHHHCC
NYSSLAVQAAMANLLTDKYAEGFPHHRYYGGCQNVDSIESAAAAEACALFGAEHAYVQPH
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCC
SGADANLVAFWAILSRQIEMPTLSSLGVTAATHLSEEQWEVLRQKMGNQKLMGLDYFSGG
CCCCCHHHHHHHHHHHHCCCCCCHHCCCHHHHHCCHHHHHHHHHHCCCCEEEEEEECCCC
HLTHGYRQNVSGRMFRVVSYAVDRDTGLLDYAAIEAQAKRERPLILLAGYSAYPRSINFR
CCCCHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHH
IFREIADKVGAVLMADMAHFAGLVAGGVFTGDEDPVRWSHIVTSTTHKTLRGPRGAFILC
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEE
KKEFAEAVDKGCPLVLGGPLPHVMAAKAVAFREARNAAFKTYAHAVRDNARALADACIQQ
HHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GMQLQTGGTDNHLLLLDVRPFGLTGRQAERALIDCGVTLNRNSLPFDPNGAWLTSGLRIG
CCEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCEECCCCCCCCCCCCEEECCCEEC
TPAVTSLGMGPEEMKRIARLIARVLGAATPVRTKTGALSKSAAEVPGEVRSSVCSEVREL
CCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHH
LARFTLYPELDEPFLRAHFTRRPAGQNTCRRRDLNPYGVTPTDFESVVSASFTTSARAQP
HHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
ITRGTKGTAVHVVFLREAPCLPLREPLFFSHEEF
CCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876