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The map label for this gene is pyrG

Identifier: 189025539

GI number: 189025539

Start: 322152

End: 323885

Strand: Direct

Name: pyrG

Synonym: TPASS_0305

Alternate gene names: 189025539

Gene position: 322152-323885 (Clockwise)

Preceding gene: 189025536

Following gene: 189025540

Centisome position: 28.27

GC content: 58.25

Gene sequence:

>1734_bases
ATGGATCCGGCTTTTATCTTTATTACAGGCGGTGTAGTTTCCTCGCTGGGCAAGGGTATTGCCGCAGGTGCCATCGGACT
TTTGCTTAAGAGCCGGGGTATATCCGTGGTGAATCAAAAGTTTGATCCCTACCTGAATGGGGATCCCGGTACCATGAATC
CCTATCAGCACGGGGAGGTGTTTGTCACGCAAGATGGCGGCGAAACGGACCTGGATCTGGGCCATTACGAGCGCTTCACG
GACGTGCCTTCAAGTAGATTCAATAGCACGACTGCGGGGAGCGTGTACCGGGCTATTTTGGACCGTGAACGCGCGGGAGG
ATACGGAGGTGCTACCGTACAGGTTATTCCTCACGTTACGGATGAAATCCAGGCGCGAATTCGCGCCGCTGCTGCTACCA
CGGGGGCTCGGGTGGTTATCACCGAAATCGGTGGTACCGTCGGTGACATAGAGTCGCTTCCGTTCATCGAGGCGATTCGT
CAAATCAGGCGTGTTCTGGGGAAGGAGCGCTGTCTTTTTATTCATTTGGGTTTAGTTCCCTACTTACCCAGTTGTGGTGA
AATGAAAACCAAGCCGCTACAGCACAGCGTAAAGGAGCTGCTAGGACTTGGTGTGCAGCCGGATGTCATCCTGTGCCGCA
GCGAGCGTCACATCACGGATGCGGTGCGCGAAAAGCTGAGCCTTTTTTGCAATGTTGAGCGGCGTGCTATTGTCGAGAAC
GTGACGGCTCGTTCTATCTATGAGGTGCCGCTCCTTTTGGAAGCAGAAGGGCTGGGAGCGCTCTTGTGTGAGCGTTTGCG
TCTTTTTGATACCTGCTGCGGGGGGCAGGTGGCGCGTAATCTCGGTGCAGGAGGCGCGCAGTCGGCTGTGTTGGGAGCGG
GGGGGACGGTCCGTACAGACGGTGGTCTGCATCCTGCAGCAGGGCAGGGGGCAGAGCCAGATCTTACGGCGTGGCGCGCA
ATGGTCCGTGCACTGTACTATCCGCGGCGGGAGCTTACGGTGGCGCTGGTGGGTAAGTATGTGTCGCTTGCGGACGCGTA
TTTGAGCGTGAGCGAAGCGTTGACGGCGGCGGGTATTTGCCATCGTGCGCGAGTGGACATGCGCTGGATAGACGCAGAGG
AAATCTGCAGCGTGCAGGACGCTGGGCACGCTCTTGCGGACGCAGATGCGCTGGTAATTCCAGGTGGCTTTGGCGTGCGG
GGGATTGAAGGGATGATCTGTGCAGTTTCGCACGCGCGTGTGCAGAACCTGCCCTATCTTGGCATTTGCTTGGGGATGCA
GATTGCAGTCATTGAATTTGCGCGCAATGTACTGTTGCTGGCAAGTGCACACTCTCGAGAATTTGCGGTAGACACCCCTC
ACCCGGTTGTTGATCTTTTGCCCGGTTGTGTAGATACCCCAACGGGTGGAAGCCTGCGTCTGGGACAGTATCGCTGTCTG
CTTGCGGAAGGTTCGCGCGCGCGTGCACTGTACGGTAGGGGGGAGGTCTGGGAGCGGCACCGGCACCGCTATGGGTTGAA
CGCGGCGTATCGAGCGCGCTTTGAGGCGAGCGCGTTGCGTCCGGTGGGAGTGGATTCAGACTGTGGCGCAGTGGAGGTGG
TGGAGCACGGGGAGCATCCGTGGTTTTTCGGCGTGCAGTTCCACCCGGAATTTTGTTCTCGACCGAACCGGGCTCACCCT
TTGTTCCGGGCGCTGGTGGCGGCGGGCTTGGAGCGAAAAGACAGTCGTTCTTGA

Upstream 100 bases:

>100_bases
TGTTCGATGCAAGCCGATGGGACAGTCCACCCTACAGGCGCAGGGGAGAGGCAGGGTGCTCCCTTTTAAAAAACGCATGG
ACACGCTACACTCGTGCCGT

Downstream 100 bases:

>100_bases
CGCGCGCAGGTATTTTTTCTAGACTCCTCTGTCCTTCTCAGCCGGTTGAAAGAGCTTTGTGTGGGCGTGCGGGTTTTGTT
TGCGCGCGCTCCGATGCGGT

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MDPAFIFITGGVVSSLGKGIAAGAIGLLLKSRGISVVNQKFDPYLNGDPGTMNPYQHGEVFVTQDGGETDLDLGHYERFT
DVPSSRFNSTTAGSVYRAILDRERAGGYGGATVQVIPHVTDEIQARIRAAAATTGARVVITEIGGTVGDIESLPFIEAIR
QIRRVLGKERCLFIHLGLVPYLPSCGEMKTKPLQHSVKELLGLGVQPDVILCRSERHITDAVREKLSLFCNVERRAIVEN
VTARSIYEVPLLLEAEGLGALLCERLRLFDTCCGGQVARNLGAGGAQSAVLGAGGTVRTDGGLHPAAGQGAEPDLTAWRA
MVRALYYPRRELTVALVGKYVSLADAYLSVSEALTAAGICHRARVDMRWIDAEEICSVQDAGHALADADALVIPGGFGVR
GIEGMICAVSHARVQNLPYLGICLGMQIAVIEFARNVLLLASAHSREFAVDTPHPVVDLLPGCVDTPTGGSLRLGQYRCL
LAEGSRARALYGRGEVWERHRHRYGLNAAYRARFEASALRPVGVDSDCGAVEVVEHGEHPWFFGVQFHPEFCSRPNRAHP
LFRALVAAGLERKDSRS

Sequences:

>Translated_577_residues
MDPAFIFITGGVVSSLGKGIAAGAIGLLLKSRGISVVNQKFDPYLNGDPGTMNPYQHGEVFVTQDGGETDLDLGHYERFT
DVPSSRFNSTTAGSVYRAILDRERAGGYGGATVQVIPHVTDEIQARIRAAAATTGARVVITEIGGTVGDIESLPFIEAIR
QIRRVLGKERCLFIHLGLVPYLPSCGEMKTKPLQHSVKELLGLGVQPDVILCRSERHITDAVREKLSLFCNVERRAIVEN
VTARSIYEVPLLLEAEGLGALLCERLRLFDTCCGGQVARNLGAGGAQSAVLGAGGTVRTDGGLHPAAGQGAEPDLTAWRA
MVRALYYPRRELTVALVGKYVSLADAYLSVSEALTAAGICHRARVDMRWIDAEEICSVQDAGHALADADALVIPGGFGVR
GIEGMICAVSHARVQNLPYLGICLGMQIAVIEFARNVLLLASAHSREFAVDTPHPVVDLLPGCVDTPTGGSLRLGQYRCL
LAEGSRARALYGRGEVWERHRHRYGLNAAYRARFEASALRPVGVDSDCGAVEVVEHGEHPWFFGVQFHPEFCSRPNRAHP
LFRALVAAGLERKDSRS
>Mature_577_residues
MDPAFIFITGGVVSSLGKGIAAGAIGLLLKSRGISVVNQKFDPYLNGDPGTMNPYQHGEVFVTQDGGETDLDLGHYERFT
DVPSSRFNSTTAGSVYRAILDRERAGGYGGATVQVIPHVTDEIQARIRAAAATTGARVVITEIGGTVGDIESLPFIEAIR
QIRRVLGKERCLFIHLGLVPYLPSCGEMKTKPLQHSVKELLGLGVQPDVILCRSERHITDAVREKLSLFCNVERRAIVEN
VTARSIYEVPLLLEAEGLGALLCERLRLFDTCCGGQVARNLGAGGAQSAVLGAGGTVRTDGGLHPAAGQGAEPDLTAWRA
MVRALYYPRRELTVALVGKYVSLADAYLSVSEALTAAGICHRARVDMRWIDAEEICSVQDAGHALADADALVIPGGFGVR
GIEGMICAVSHARVQNLPYLGICLGMQIAVIEFARNVLLLASAHSREFAVDTPHPVVDLLPGCVDTPTGGSLRLGQYRCL
LAEGSRARALYGRGEVWERHRHRYGLNAAYRARFEASALRPVGVDSDCGAVEVVEHGEHPWFFGVQFHPEFCSRPNRAHP
LFRALVAAGLERKDSRS

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=586, Percent_Identity=40.9556313993174, Blast_Score=438, Evalue=1e-123,
Organism=Homo sapiens, GI28559085, Length=582, Percent_Identity=40.893470790378, Blast_Score=435, Evalue=1e-122,
Organism=Homo sapiens, GI28559083, Length=582, Percent_Identity=40.893470790378, Blast_Score=435, Evalue=1e-122,
Organism=Homo sapiens, GI221316689, Length=582, Percent_Identity=40.893470790378, Blast_Score=435, Evalue=1e-122,
Organism=Escherichia coli, GI1789142, Length=582, Percent_Identity=45.1890034364261, Blast_Score=495, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI25148299, Length=637, Percent_Identity=35.0078492935636, Blast_Score=367, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6319432, Length=585, Percent_Identity=38.2905982905983, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6322563, Length=587, Percent_Identity=38.8415672913118, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24664469, Length=584, Percent_Identity=40.2397260273973, Blast_Score=420, Evalue=1e-117,
Organism=Drosophila melanogaster, GI21357815, Length=531, Percent_Identity=38.7947269303202, Blast_Score=356, Evalue=2e-98,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_TREPA (O83327)

Other databases:

- EMBL:   AE000520
- PIR:   C71342
- RefSeq:   NP_218746.1
- ProteinModelPortal:   O83327
- SMR:   O83327
- IntAct:   O83327
- MINT:   MINT-6479806
- GeneID:   2610727
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0305
- NMPDR:   fig|243276.1.peg.305
- TIGR:   TP_0305
- HOGENOM:   HBG597806
- OMA:   RVTMQKL
- ProtClustDB:   PRK05380
- BioCyc:   TPAL243276:TP_0305-MONOMER
- BRENDA:   6.3.4.2
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 62148; Mature: 62148

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 423-423 ACT_SITE 548-548 ACT_SITE 550-550

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPAFIFITGGVVSSLGKGIAAGAIGLLLKSRGISVVNQKFDPYLNGDPGTMNPYQHGEV
CCCEEEEEECHHHHHHCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCEE
FVTQDGGETDLDLGHYERFTDVPSSRFNSTTAGSVYRAILDRERAGGYGGATVQVIPHVT
EEECCCCCCCCCCHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCH
DEIQARIRAAAATTGARVVITEIGGTVGDIESLPFIEAIRQIRRVLGKERCLFIHLGLVP
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECHHH
YLPSCGEMKTKPLQHSVKELLGLGVQPDVILCRSERHITDAVREKLSLFCNVERRAIVEN
CCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHH
VTARSIYEVPLLLEAEGLGALLCERLRLFDTCCGGQVARNLGAGGAQSAVLGAGGTVRTD
HHHHHHEECCEEEECCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCEEEECCCEEECC
GGLHPAAGQGAEPDLTAWRAMVRALYYPRRELTVALVGKYVSLADAYLSVSEALTAAGIC
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HRARVDMRWIDAEEICSVQDAGHALADADALVIPGGFGVRGIEGMICAVSHARVQNLPYL
HHHCCCEEECCHHHHHHHHHCCCHHHCCCEEEECCCCCCCCCCHHEEEHHHHHHHCCCEE
GICLGMQIAVIEFARNVLLLASAHSREFAVDTPHPVVDLLPGCVDTPTGGSLRLGQYRCL
EHHHHHHHHHHHHHCCEEEEEECCCCCEECCCCCHHHHHCCCCCCCCCCCCEEECCEEEE
LAEGSRARALYGRGEVWERHRHRYGLNAAYRARFEASALRPVGVDSDCGAVEVVEHGEHP
EECCCCCEEECCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCEEHHEECCCCC
WFFGVQFHPEFCSRPNRAHPLFRALVAAGLERKDSRS
EEEEEEECHHHHCCCCCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDPAFIFITGGVVSSLGKGIAAGAIGLLLKSRGISVVNQKFDPYLNGDPGTMNPYQHGEV
CCCEEEEEECHHHHHHCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCEE
FVTQDGGETDLDLGHYERFTDVPSSRFNSTTAGSVYRAILDRERAGGYGGATVQVIPHVT
EEECCCCCCCCCCHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCH
DEIQARIRAAAATTGARVVITEIGGTVGDIESLPFIEAIRQIRRVLGKERCLFIHLGLVP
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECHHH
YLPSCGEMKTKPLQHSVKELLGLGVQPDVILCRSERHITDAVREKLSLFCNVERRAIVEN
CCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHH
VTARSIYEVPLLLEAEGLGALLCERLRLFDTCCGGQVARNLGAGGAQSAVLGAGGTVRTD
HHHHHHEECCEEEECCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCEEEECCCEEECC
GGLHPAAGQGAEPDLTAWRAMVRALYYPRRELTVALVGKYVSLADAYLSVSEALTAAGIC
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HRARVDMRWIDAEEICSVQDAGHALADADALVIPGGFGVRGIEGMICAVSHARVQNLPYL
HHHCCCEEECCHHHHHHHHHCCCHHHCCCEEEECCCCCCCCCCHHEEEHHHHHHHCCCEE
GICLGMQIAVIEFARNVLLLASAHSREFAVDTPHPVVDLLPGCVDTPTGGSLRLGQYRCL
EHHHHHHHHHHHHHCCEEEEEECCCCCEECCCCCHHHHHCCCCCCCCCCCCEEECCEEEE
LAEGSRARALYGRGEVWERHRHRYGLNAAYRARFEASALRPVGVDSDCGAVEVVEHGEHP
EECCCCCEEECCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCEEHHEECCCCC
WFFGVQFHPEFCSRPNRAHPLFRALVAAGLERKDSRS
EEEEEEECHHHHCCCCCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876