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The map label for this gene is rho

Identifier: 189025488

GI number: 189025488

Start: 266700

End: 268259

Strand: Direct

Name: rho

Synonym: TPASS_0254

Alternate gene names: 189025488

Gene position: 266700-268259 (Clockwise)

Preceding gene: 189025487

Following gene: 189025489

Centisome position: 23.41

GC content: 48.97

Gene sequence:

>1560_bases
ATGAAAGTTTCTGTTGGTACCGTCGTCGGACAAGGAGAGTTGGGTTTGGAAGAGGGAATCGTGGGGAATCAGATTGTGTC
TGAGGATAGTGCCGCTGACGTGGGGGAATGCGATGCCTACGGCGGGAGGAAGAAGCTCGTCATCCGCTCTCGCTCACGGA
GACGAGTGGTGACGCCAAAAGCGCTGGAGGTGGAAAAACGTCCGCGAAGAGCCCTACGCTTTAAGCAAAATTTCTGCCCA
GAAGATGCGGAAACAAGTGAGTCTTCCGCCGACGTTAAGGGTAGGGCGCGTTTGCTGATTGATGATTTAACTGAGATGGG
GATGCATGCCCTGCGTGATTTTGCAGTACAGCGGGGTATCTCTCAAGAAGACGTGGTTGTCATGAAAAAGTCAGATATCA
TCTTTCACGTTCTTAAAAATCACACTGAGAATGGTGGCGTTATCTTTGCTTCTGGCTCGCTGGAGATATTGCCTGACGGG
TATGGTTTTTTGCGTTCACCTCAGAACAGTTATTTGCCCGGTTCCGATGACATCTACGTCTCTCCAAGTCAGATTCGCCT
GTTTAATCTGAAAACGGGCGACACCATCTATGGGCAGATTCGCTCCCCTAAGGAGGGAGAGCGTTTCTTTGCAATGCTCC
GCGTGGAAACGGTAAACTTCGAAGAAGTAGCAAAGGTACAGGACCGTGTCCCTTTCGACAGTCTTACCCCTTTGTACCCG
CGGGAAAAGCTGAATTTAGAAACAAGAACGGAAGATATCTCAACGCGTGTCATGAACTTGTTCTGTCCGATCGGAAAGGG
GCAGCGTGCGCTGATTGTAGCGCCGCCGCGTACGGGAAAGACGATTCTCATGCAGAAAATTGCAAATGCCATCACGCAAA
ATCATCCTGAAGTTTATCTTATCGTGCTGCTCATCGATGAGCGTCCTGAAGAGGTAACCGACATGGAGCGTACGGTTGAT
GCTGAAGTTATTTCTTCTACGTTCGATGAACAGGCTACTCGCCATGTACAGATTGCAGAGATAGTGCTTGAGCGCGCAAA
GCGATTGGTAGAGCACCGGAAGGACGTGGTGATTTTGCTTGACTCGATTACGCGTCTGGCGCGTGCCTATAACCAAACGA
TGCCAACTTCAGGTAAAGTGTTGTCGGGGGGAGTGGATTCAAATGCTCTGCACAAACCGAAACGCTTCTTTGGTGCAGCT
CGCAATGTGGAGGAAGGAGGAAGTCTGACAATCATTGCCACTGCGCTTATTGAAACAGGCAGTAGGATGGATGAGGTTAT
TTTTGAGGAGTTTAAAGGTACGGGGAATATGGAGATAAATCTCGATCGTCGCCTTTCGGATCGGCGCCTATTTCCCGCGG
TGAGTATTAAAAAGTCTGGTACGCGTAAGGAAGAGTTGCTCCTGACGGAGGAAGAAATGCAGCGTATTTGGGTGTTGCGT
AAGGTAATCAATCCTATGGACGATGCAGAAATTGTGGAATTGTTAATTGATAAGATGCGTAAAACAAAGAACAACGAGGC
ATTTTTGAGGTCTATGAATACGGGTGCGTTCTCCTCCTGA

Upstream 100 bases:

>100_bases
GGCGTGTACTCGTTGGACGTCGGTTTTTCTTTCCAGGGTTGTAGCGTGCACGGTGCTGCGTGCTGTTCAAACCGGTGTCG
GTAATCTCGGTGTGTAAGTT

Downstream 100 bases:

>100_bases
GTAAGGAGACTGGAACGTGTGTCGGTTACACCCGGCGTGTACGAGCCTTCTGGAAGAGGGTGGATGTAACCTTTTTAAGG
GCGCGGCGCGCTGTAGAATG

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MKVSVGTVVGQGELGLEEGIVGNQIVSEDSAADVGECDAYGGRKKLVIRSRSRRRVVTPKALEVEKRPRRALRFKQNFCP
EDAETSESSADVKGRARLLIDDLTEMGMHALRDFAVQRGISQEDVVVMKKSDIIFHVLKNHTENGGVIFASGSLEILPDG
YGFLRSPQNSYLPGSDDIYVSPSQIRLFNLKTGDTIYGQIRSPKEGERFFAMLRVETVNFEEVAKVQDRVPFDSLTPLYP
REKLNLETRTEDISTRVMNLFCPIGKGQRALIVAPPRTGKTILMQKIANAITQNHPEVYLIVLLIDERPEEVTDMERTVD
AEVISSTFDEQATRHVQIAEIVLERAKRLVEHRKDVVILLDSITRLARAYNQTMPTSGKVLSGGVDSNALHKPKRFFGAA
RNVEEGGSLTIIATALIETGSRMDEVIFEEFKGTGNMEINLDRRLSDRRLFPAVSIKKSGTRKEELLLTEEEMQRIWVLR
KVINPMDDAEIVELLIDKMRKTKNNEAFLRSMNTGAFSS

Sequences:

>Translated_519_residues
MKVSVGTVVGQGELGLEEGIVGNQIVSEDSAADVGECDAYGGRKKLVIRSRSRRRVVTPKALEVEKRPRRALRFKQNFCP
EDAETSESSADVKGRARLLIDDLTEMGMHALRDFAVQRGISQEDVVVMKKSDIIFHVLKNHTENGGVIFASGSLEILPDG
YGFLRSPQNSYLPGSDDIYVSPSQIRLFNLKTGDTIYGQIRSPKEGERFFAMLRVETVNFEEVAKVQDRVPFDSLTPLYP
REKLNLETRTEDISTRVMNLFCPIGKGQRALIVAPPRTGKTILMQKIANAITQNHPEVYLIVLLIDERPEEVTDMERTVD
AEVISSTFDEQATRHVQIAEIVLERAKRLVEHRKDVVILLDSITRLARAYNQTMPTSGKVLSGGVDSNALHKPKRFFGAA
RNVEEGGSLTIIATALIETGSRMDEVIFEEFKGTGNMEINLDRRLSDRRLFPAVSIKKSGTRKEELLLTEEEMQRIWVLR
KVINPMDDAEIVELLIDKMRKTKNNEAFLRSMNTGAFSS
>Mature_519_residues
MKVSVGTVVGQGELGLEEGIVGNQIVSEDSAADVGECDAYGGRKKLVIRSRSRRRVVTPKALEVEKRPRRALRFKQNFCP
EDAETSESSADVKGRARLLIDDLTEMGMHALRDFAVQRGISQEDVVVMKKSDIIFHVLKNHTENGGVIFASGSLEILPDG
YGFLRSPQNSYLPGSDDIYVSPSQIRLFNLKTGDTIYGQIRSPKEGERFFAMLRVETVNFEEVAKVQDRVPFDSLTPLYP
REKLNLETRTEDISTRVMNLFCPIGKGQRALIVAPPRTGKTILMQKIANAITQNHPEVYLIVLLIDERPEEVTDMERTVD
AEVISSTFDEQATRHVQIAEIVLERAKRLVEHRKDVVILLDSITRLARAYNQTMPTSGKVLSGGVDSNALHKPKRFFGAA
RNVEEGGSLTIIATALIETGSRMDEVIFEEFKGTGNMEINLDRRLSDRRLFPAVSIKKSGTRKEELLLTEEEMQRIWVLR
KVINPMDDAEIVELLIDKMRKTKNNEAFLRSMNTGAFSS

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family

Homologues:

Organism=Escherichia coli, GI1790217, Length=393, Percent_Identity=63.1043256997455, Blast_Score=533, Evalue=1e-152,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): RHO_TREPA (O83281)

Other databases:

- EMBL:   AE000520
- PIR:   C71346
- RefSeq:   NP_218695.1
- ProteinModelPortal:   O83281
- SMR:   O83281
- GeneID:   2611493
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0254
- NMPDR:   fig|243276.1.peg.254
- TIGR:   TP_0254
- HOGENOM:   HBG335262
- OMA:   YNYLPGP
- ProtClustDB:   PRK09376
- BioCyc:   TPAL243276:TP_0254-MONOMER
- HAMAP:   MF_01884
- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00382
- SMART:   SM00357
- SMART:   SM00959
- TIGRFAMs:   TIGR00767

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind; SSF50249 Nucleic_acid_OB

EC number: NA

Molecular weight: Translated: 58267; Mature: 58267

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: BINDING 308-308

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSVGTVVGQGELGLEEGIVGNQIVSEDSAADVGECDAYGGRKKLVIRSRSRRRVVTPK
CEEEEEEEEECCCCCHHCCCCCCHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCEECCCC
ALEVEKRPRRALRFKQNFCPEDAETSESSADVKGRARLLIDDLTEMGMHALRDFAVQRGI
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
SQEDVVVMKKSDIIFHVLKNHTENGGVIFASGSLEILPDGYGFLRSPQNSYLPGSDDIYV
CCCCEEEEECCCHHHHHHHCCCCCCCEEEECCCEEECCCCCHHHCCCCCCCCCCCCCEEE
SPSQIRLFNLKTGDTIYGQIRSPKEGERFFAMLRVETVNFEEVAKVQDRVPFDSLTPLYP
CCCEEEEEEECCCCEEEEECCCCCCCCCEEEEEEEEECCHHHHHHHHHCCCCCCCCCCCC
REKLNLETRTEDISTRVMNLFCPIGKGQRALIVAPPRTGKTILMQKIANAITQNHPEVYL
HHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEE
IVLLIDERPEEVTDMERTVDAEVISSTFDEQATRHVQIAEIVLERAKRLVEHRKDVVILL
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEH
DSITRLARAYNQTMPTSGKVLSGGVDSNALHKPKRFFGAARNVEEGGSLTIIATALIETG
HHHHHHHHHHHCCCCCCCCEEECCCCCCHHCCCHHHHHHHCCCCCCCCEEEEEEHHHHCC
SRMDEVIFEEFKGTGNMEINLDRRLSDRRLFPAVSIKKSGTRKEELLLTEEEMQRIWVLR
CHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEECCCCCCHHHHEECHHHHHHHHHHH
KVINPMDDAEIVELLIDKMRKTKNNEAFLRSMNTGAFSS
HHHCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure
MKVSVGTVVGQGELGLEEGIVGNQIVSEDSAADVGECDAYGGRKKLVIRSRSRRRVVTPK
CEEEEEEEEECCCCCHHCCCCCCHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCEECCCC
ALEVEKRPRRALRFKQNFCPEDAETSESSADVKGRARLLIDDLTEMGMHALRDFAVQRGI
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
SQEDVVVMKKSDIIFHVLKNHTENGGVIFASGSLEILPDGYGFLRSPQNSYLPGSDDIYV
CCCCEEEEECCCHHHHHHHCCCCCCCEEEECCCEEECCCCCHHHCCCCCCCCCCCCCEEE
SPSQIRLFNLKTGDTIYGQIRSPKEGERFFAMLRVETVNFEEVAKVQDRVPFDSLTPLYP
CCCEEEEEEECCCCEEEEECCCCCCCCCEEEEEEEEECCHHHHHHHHHCCCCCCCCCCCC
REKLNLETRTEDISTRVMNLFCPIGKGQRALIVAPPRTGKTILMQKIANAITQNHPEVYL
HHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEE
IVLLIDERPEEVTDMERTVDAEVISSTFDEQATRHVQIAEIVLERAKRLVEHRKDVVILL
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEH
DSITRLARAYNQTMPTSGKVLSGGVDSNALHKPKRFFGAARNVEEGGSLTIIATALIETG
HHHHHHHHHHHCCCCCCCCEEECCCCCCHHCCCHHHHHHHCCCCCCCCEEEEEEHHHHCC
SRMDEVIFEEFKGTGNMEINLDRRLSDRRLFPAVSIKKSGTRKEELLLTEEEMQRIWVLR
CHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEECCCCCCHHHHEECHHHHHHHHHHH
KVINPMDDAEIVELLIDKMRKTKNNEAFLRSMNTGAFSS
HHHCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876