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The map label for this gene is amiA [H]

Identifier: 189025480

GI number: 189025480

Start: 261005

End: 262078

Strand: Direct

Name: amiA [H]

Synonym: TPASS_0247

Alternate gene names: 189025480

Gene position: 261005-262078 (Clockwise)

Preceding gene: 189025479

Following gene: 189025481

Centisome position: 22.91

GC content: 48.23

Gene sequence:

>1074_bases
GTGCATTGGTACTTTGACAGGGTATCTCGAGCGAGGGGCATTATGAAGTTTTTGCGTTCGTGTTTGATAGGTGGTGTTTT
CTTTTTTGCACTGTGTCTTTCTGCTGATGAAGCGTTTGTGTCTGCGATGAAGCTTGCGCGTACGTTGAATGTGTCGGTTA
GTTGGAATCCGCTTTCGCAAACAATTTTATTTTCCAAAGGTGAGCGACGAGTACAATGTCGGGTTGGGCAAGTGCTTGCA
CTATTGGATGGTAGTGAGGTGCTGATGATTGATCCGCCGGTGGTAAAGGACGCGGTGGTGTATGTAAGTGGAGGGTTTGC
AGAGCGGCTAAGGAAAGTATTTGGGTATTCTTCACATCAACCTGAGCATCGTGTGGGTGCGGTGCTCATAGACCCAGGAC
ATGGAGGAAAGGATTGGGGGACGAAGGGCTCGTACCGTGAGCAGGGTAAAACGGTGGTGGTGAAAGAAAAAGACATTGCG
TTGCGTGCATCTCAAAATATCTATGACTTACTTACGGCGCGCTATCCGGATCGTAAAATCATTATGACGCGGAAAGGGGA
TTCATATCTTACGCTTGAGGAGCGTGTGGCGATGGCAAATGGGGTAAAGCTAGGGAAGTACGAAGCGATCCTGTACGTGT
CGGTGCATGCGAATTTTTCATGGAATACAAAGGCTTCTGGGTTTGAGGTGTGGTACTTGCCGCCTGAATATCGCAGGTCA
ATTCTTGATAAAAATGCGGCGTCCAAAGAGGTGCTCCCGATCTTGAACTCGATGCTCGAAGAGGAATTCACAATGGAAAG
TATTATGATTGCGCGCAGTATCGCTGACGGAATGCAAGCAAGCGTCGGTGCACAAAGTAAAAATCGTGGAGTAAAAGAGG
AGGCCTGGTTTGTAGTACGGAACGCGAAGATGCCAAGTGTTTTGGTGGAATTAGGCTTTGTGAGTAATCCGGTAGAGGCG
CGGTTGCTCAACGATGCGGACTACTTGAAAAGGTGCGCGCAGGGAATCTACAATGGGCTTGTTTCTTTTATCACGTATTT
TGAAGGTTCGGGGGGATTTACCGGACCCTTATGA

Upstream 100 bases:

>100_bases
TGCACCGGTTGTCATCTGGTGAGAGATTGCATGCGCAAAGGGTATGGTAGTGTGGTGCGTGGACGTATGTGCTTATTGTG
AGTAGGGAGAGGGGACAGGT

Downstream 100 bases:

>100_bases
TAGGGGATGGGGCAGCATTGTACGGTGTAGTGAGGGCGGTGTGTTGGACTCTCTTGCTAATAATTGGTGTGGCACTTGCG
GTGCAGCAATTGGCGGGGGA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MHWYFDRVSRARGIMKFLRSCLIGGVFFFALCLSADEAFVSAMKLARTLNVSVSWNPLSQTILFSKGERRVQCRVGQVLA
LLDGSEVLMIDPPVVKDAVVYVSGGFAERLRKVFGYSSHQPEHRVGAVLIDPGHGGKDWGTKGSYREQGKTVVVKEKDIA
LRASQNIYDLLTARYPDRKIIMTRKGDSYLTLEERVAMANGVKLGKYEAILYVSVHANFSWNTKASGFEVWYLPPEYRRS
ILDKNAASKEVLPILNSMLEEEFTMESIMIARSIADGMQASVGAQSKNRGVKEEAWFVVRNAKMPSVLVELGFVSNPVEA
RLLNDADYLKRCAQGIYNGLVSFITYFEGSGGFTGPL

Sequences:

>Translated_357_residues
MHWYFDRVSRARGIMKFLRSCLIGGVFFFALCLSADEAFVSAMKLARTLNVSVSWNPLSQTILFSKGERRVQCRVGQVLA
LLDGSEVLMIDPPVVKDAVVYVSGGFAERLRKVFGYSSHQPEHRVGAVLIDPGHGGKDWGTKGSYREQGKTVVVKEKDIA
LRASQNIYDLLTARYPDRKIIMTRKGDSYLTLEERVAMANGVKLGKYEAILYVSVHANFSWNTKASGFEVWYLPPEYRRS
ILDKNAASKEVLPILNSMLEEEFTMESIMIARSIADGMQASVGAQSKNRGVKEEAWFVVRNAKMPSVLVELGFVSNPVEA
RLLNDADYLKRCAQGIYNGLVSFITYFEGSGGFTGPL
>Mature_357_residues
MHWYFDRVSRARGIMKFLRSCLIGGVFFFALCLSADEAFVSAMKLARTLNVSVSWNPLSQTILFSKGERRVQCRVGQVLA
LLDGSEVLMIDPPVVKDAVVYVSGGFAERLRKVFGYSSHQPEHRVGAVLIDPGHGGKDWGTKGSYREQGKTVVVKEKDIA
LRASQNIYDLLTARYPDRKIIMTRKGDSYLTLEERVAMANGVKLGKYEAILYVSVHANFSWNTKASGFEVWYLPPEYRRS
ILDKNAASKEVLPILNSMLEEEFTMESIMIARSIADGMQASVGAQSKNRGVKEEAWFVVRNAKMPSVLVELGFVSNPVEA
RLLNDADYLKRCAQGIYNGLVSFITYFEGSGGFTGPL

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm. Note=Distributed throughout the periplasm in all cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=272, Percent_Identity=28.3088235294118, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87082163, Length=231, Percent_Identity=27.7056277056277, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1790611, Length=239, Percent_Identity=29.7071129707113, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 39810; Mature: 39810

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHWYFDRVSRARGIMKFLRSCLIGGVFFFALCLSADEAFVSAMKLARTLNVSVSWNPLSQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEEEEECCHHH
TILFSKGERRVQCRVGQVLALLDGSEVLMIDPPVVKDAVVYVSGGFAERLRKVFGYSSHQ
HEEECCCCCEEEEHHHHEEEEECCCEEEEECCCCCCCEEEEEECCHHHHHHHHHCCCCCC
PEHRVGAVLIDPGHGGKDWGTKGSYREQGKTVVVKEKDIALRASQNIYDLLTARYPDRKI
CCCCCCEEEECCCCCCCCCCCCCCHHHCCCEEEEEECCEEEECCCCHHHHHHCCCCCCEE
IMTRKGDSYLTLEERVAMANGVKLGKYEAILYVSVHANFSWNTKASGFEVWYLPPEYRRS
EEEECCCCEEEHHHHHHHHCCCEECCEEEEEEEEEECCEEECCCCCCEEEEECCHHHHHH
ILDKNAASKEVLPILNSMLEEEFTMESIMIARSIADGMQASVGAQSKNRGVKEEAWFVVR
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCHHHEEEEE
NAKMPSVLVELGFVSNPVEARLLNDADYLKRCAQGIYNGLVSFITYFEGSGGFTGPL
CCCCCHHHHHHCCCCCCHHHEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MHWYFDRVSRARGIMKFLRSCLIGGVFFFALCLSADEAFVSAMKLARTLNVSVSWNPLSQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEEEEECCHHH
TILFSKGERRVQCRVGQVLALLDGSEVLMIDPPVVKDAVVYVSGGFAERLRKVFGYSSHQ
HEEECCCCCEEEEHHHHEEEEECCCEEEEECCCCCCCEEEEEECCHHHHHHHHHCCCCCC
PEHRVGAVLIDPGHGGKDWGTKGSYREQGKTVVVKEKDIALRASQNIYDLLTARYPDRKI
CCCCCCEEEECCCCCCCCCCCCCCHHHCCCEEEEEECCEEEECCCCHHHHHHCCCCCCEE
IMTRKGDSYLTLEERVAMANGVKLGKYEAILYVSVHANFSWNTKASGFEVWYLPPEYRRS
EEEECCCCEEEHHHHHHHHCCCEECCEEEEEEEEEECCEEECCCCCCEEEEECCHHHHHH
ILDKNAASKEVLPILNSMLEEEFTMESIMIARSIADGMQASVGAQSKNRGVKEEAWFVVR
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCHHHEEEEE
NAKMPSVLVELGFVSNPVEARLLNDADYLKRCAQGIYNGLVSFITYFEGSGGFTGPL
CCCCCHHHHHHCCCCCCHHHEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8300522; 9205837; 9278503; 1903834 [H]