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The map label for this gene is grpE

Identifier: 189025449

GI number: 189025449

Start: 219152

End: 219814

Strand: Direct

Name: grpE

Synonym: TPASS_0215

Alternate gene names: 189025449

Gene position: 219152-219814 (Clockwise)

Preceding gene: 189025448

Following gene: 189025450

Centisome position: 19.23

GC content: 57.62

Gene sequence:

>663_bases
GTGTGCGGCGGCGACGTTCAGGGGCAGGGAGTTGCCTCAGGTTGTGACGAGGCCTTGGAGCGGGCAGATAGCCTTCGCGC
GTCTGATCCTGTACCGGTTGAATCGGGGGAGGGTTCTGTTCCTGGGGAGCATAGTCAGGAGTTGGAGACAGGTGCCTCTG
AAGAGACCCTGCGCGAGCGCGTGAATGTTTTGCAGGAGCAGTACCTGCGCAAGGCTGCCGACCTCGAAAACTACCGGAAG
CGTGCGTTGCGGGAAAGGCAGGAGGCGGTGGAACACGCGTACGCGGCGCTGCTTGCCGACATCGTCGCTGTCTTGGATGA
CTTTGACCGTGCTATTGAAGCGGCGGATCACGCGTCGAGTACAGAGGTGGAGGCTTCATCTGCCTTCCGAGAGGGTGTTC
TTATGATCCGCAAGCAGCTCTCCTCAGTGCTTGAGACAAAGTATGGTCTTGAGTATTACCCGGTGCTCGGGGAGCGCTTC
GATCCAAATCTCCATGAGGCTTTGAGTATGAGTCCTTCCGCTTCTGTGCATGAGAAGATAGTAGGGGCAGAGCTACAAAA
AGGATATAGGGTTAGGAACCGTATCCTCCGGCATGCCAAGGTTATGGTGCTCACTCCTGAAGAGCAGACAGAGCCCGATC
GTGGGGATGGCCCTTCGGAGTGA

Upstream 100 bases:

>100_bases
TAGTCAGACGGTAAATAGGTGTTTTCTTGACCGAGGGCGGCGTCTCTCGTTACTTTTACGGCATTACCGCGAGGGTGTTA
TGGCAAAAAAGGAGAAGAAA

Downstream 100 bases:

>100_bases
CAGGCAGGGTATGCTGAGAGGTCAGGATGGAGTTCTGGAGCACCGGTGCTAGGTAACGGCTATACTGCGCGCCCTGCAGG
CAGGGCGGGTATCCTATACA

Product: chaperone protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MCGGDVQGQGVASGCDEALERADSLRASDPVPVESGEGSVPGEHSQELETGASEETLRERVNVLQEQYLRKAADLENYRK
RALRERQEAVEHAYAALLADIVAVLDDFDRAIEAADHASSTEVEASSAFREGVLMIRKQLSSVLETKYGLEYYPVLGERF
DPNLHEALSMSPSASVHEKIVGAELQKGYRVRNRILRHAKVMVLTPEEQTEPDRGDGPSE

Sequences:

>Translated_220_residues
MCGGDVQGQGVASGCDEALERADSLRASDPVPVESGEGSVPGEHSQELETGASEETLRERVNVLQEQYLRKAADLENYRK
RALRERQEAVEHAYAALLADIVAVLDDFDRAIEAADHASSTEVEASSAFREGVLMIRKQLSSVLETKYGLEYYPVLGERF
DPNLHEALSMSPSASVHEKIVGAELQKGYRVRNRILRHAKVMVLTPEEQTEPDRGDGPSE
>Mature_220_residues
MCGGDVQGQGVASGCDEALERADSLRASDPVPVESGEGSVPGEHSQELETGASEETLRERVNVLQEQYLRKAADLENYRK
RALRERQEAVEHAYAALLADIVAVLDDFDRAIEAADHASSTEVEASSAFREGVLMIRKQLSSVLETKYGLEYYPVLGERF
DPNLHEALSMSPSASVHEKIVGAELQKGYRVRNRILRHAKVMVLTPEEQTEPDRGDGPSE

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI40255109, Length=151, Percent_Identity=31.1258278145695, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1788967, Length=161, Percent_Identity=32.2981366459627, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17552458, Length=178, Percent_Identity=29.2134831460674, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324806, Length=146, Percent_Identity=30.1369863013699, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_TREPA (O83245)

Other databases:

- EMBL:   AE000520
- PIR:   E71352
- RefSeq:   NP_218655.1
- ProteinModelPortal:   O83245
- SMR:   O83245
- GeneID:   2611499
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0215
- NMPDR:   fig|243276.1.peg.214
- TIGR:   TP_0215
- HOGENOM:   HBG732630
- OMA:   EVEPNSV
- ProtClustDB:   CLSK218590
- BioCyc:   TPAL243276:TP_0215-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 24207; Mature: 24207

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGGDVQGQGVASGCDEALERADSLRASDPVPVESGEGSVPGEHSQELETGASEETLRER
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHH
VNVLQEQYLRKAADLENYRKRALRERQEAVEHAYAALLADIVAVLDDFDRAIEAADHASS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TEVEASSAFREGVLMIRKQLSSVLETKYGLEYYPVLGERFDPNLHEALSMSPSASVHEKI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHCCCCCHHHHHHH
VGAELQKGYRVRNRILRHAKVMVLTPEEQTEPDRGDGPSE
HHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MCGGDVQGQGVASGCDEALERADSLRASDPVPVESGEGSVPGEHSQELETGASEETLRER
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHH
VNVLQEQYLRKAADLENYRKRALRERQEAVEHAYAALLADIVAVLDDFDRAIEAADHASS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TEVEASSAFREGVLMIRKQLSSVLETKYGLEYYPVLGERFDPNLHEALSMSPSASVHEKI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHCCCCCHHHHHHH
VGAELQKGYRVRNRILRHAKVMVLTPEEQTEPDRGDGPSE
HHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876