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The map label for this gene is rpoA

Identifier: 189025446

GI number: 189025446

Start: 217186

End: 218241

Strand: Direct

Name: rpoA

Synonym: TPASS_0212

Alternate gene names: 189025446

Gene position: 217186-218241 (Clockwise)

Preceding gene: 189025445

Following gene: 189025447

Centisome position: 19.06

GC content: 49.34

Gene sequence:

>1056_bases
ATGCCTCGTAGAAATCTTTTGAAGGGTTTTAAAAGACCTAAGGTGCTGGAGTTTCTTTCGGAGAACTCAAGCGAGTGTTA
TGGGAAGTTCACCGCCTCTCCTTTTGAGACTGGTTTTGGCACCACTGTTGGTAACTGTTTGCGGCGCGTCTTACTCTCTT
CTATCCAGGGGTATGCGGTCACCGGGGTTCGCATCACGTCCTTTGATGCGGACGGGGTTGCGCACTTCATTTCAAGCGAG
TTTGAACAGATTCCCCACGTACGGGAAGATACCCTCGAGATTCTAAATAATTTTAAGCGTCTGCGTTTTCTCCTGCCGCA
GGGGGCAGAGTCTAGTACGTTCACGTATGAGTTTCGCGGCGCGGTGTCTTTGACGGGGAAGGACTTTGCTAAGAAGTTTC
AACTCGAGGTTCTGTCTCAAGACCTGCTCATCATGGAAATGATGGACGGTGCGCATGTTGAAGTAGAGCTACACGTCGAA
TTCGGGCGTGGGTATGTACCTGCTGAATCGCACGATCGGTATGCCGATTTAGTTGGGGTTATCCCTGTTGACGCAATTTT
TAGTCCCGTGTTGAGAGTCCGCTATGATATTCAGTCTTGCCGTGTAGGTCAGCGGGGGGATTACGATCAGTTATCCCTTG
AAGTGTGGACAGATGGTACGGTGCGTCCCGAAGACGCGATAGCCGAGGCAGCGAAAATTATCAAGGAGCACTTTACAGTT
TTTGTTAATTTTGACGAGACCGCGCTCGACCTGGAGGACGAGCCAGAAGAGGATGACCCTGCCGTTCTGGAGCTGTTGAA
CACGAAAATCGCTGATGTAGATTTTTCAGTGCGCGCGCGTAACTGCCTTTTAACTATGGGAATCAAGACGCTGGGGGAGT
TGACAAGGATTTCTGAGCAGACACTTGCGAATACGCGTAATGTGGGTAAGAAAAGTTTAAGTGAGATACAGGGCAAGTTG
CAGGAATATAACTTGCGTCTGGGTATGGCTGACTACAACCATGTGGGGGTTGTTAGTAGACTGATGCGACAGAAGGAAGA
AATAGATGAGGCATAG

Upstream 100 bases:

>100_bases
CCATGGGACTGAGGGTGCGTTCGATTCGCGACATCACACCCATTCCACATAACGGCTGTCGTCCGCGTAAAACTCGCCGC
ATCTGATAAAAGGAGTGAGC

Downstream 100 bases:

>100_bases
GACCGGTTTCAACCCGCTTTCGTGTATGGCTGCGCATAGGCGTGCGCTCCGTCGCAATATGGTTACTTCTCTTTTTAAGT
TTGAGCGGATCACCACGACG

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MPRRNLLKGFKRPKVLEFLSENSSECYGKFTASPFETGFGTTVGNCLRRVLLSSIQGYAVTGVRITSFDADGVAHFISSE
FEQIPHVREDTLEILNNFKRLRFLLPQGAESSTFTYEFRGAVSLTGKDFAKKFQLEVLSQDLLIMEMMDGAHVEVELHVE
FGRGYVPAESHDRYADLVGVIPVDAIFSPVLRVRYDIQSCRVGQRGDYDQLSLEVWTDGTVRPEDAIAEAAKIIKEHFTV
FVNFDETALDLEDEPEEDDPAVLELLNTKIADVDFSVRARNCLLTMGIKTLGELTRISEQTLANTRNVGKKSLSEIQGKL
QEYNLRLGMADYNHVGVVSRLMRQKEEIDEA

Sequences:

>Translated_351_residues
MPRRNLLKGFKRPKVLEFLSENSSECYGKFTASPFETGFGTTVGNCLRRVLLSSIQGYAVTGVRITSFDADGVAHFISSE
FEQIPHVREDTLEILNNFKRLRFLLPQGAESSTFTYEFRGAVSLTGKDFAKKFQLEVLSQDLLIMEMMDGAHVEVELHVE
FGRGYVPAESHDRYADLVGVIPVDAIFSPVLRVRYDIQSCRVGQRGDYDQLSLEVWTDGTVRPEDAIAEAAKIIKEHFTV
FVNFDETALDLEDEPEEDDPAVLELLNTKIADVDFSVRARNCLLTMGIKTLGELTRISEQTLANTRNVGKKSLSEIQGKL
QEYNLRLGMADYNHVGVVSRLMRQKEEIDEA
>Mature_350_residues
PRRNLLKGFKRPKVLEFLSENSSECYGKFTASPFETGFGTTVGNCLRRVLLSSIQGYAVTGVRITSFDADGVAHFISSEF
EQIPHVREDTLEILNNFKRLRFLLPQGAESSTFTYEFRGAVSLTGKDFAKKFQLEVLSQDLLIMEMMDGAHVEVELHVEF
GRGYVPAESHDRYADLVGVIPVDAIFSPVLRVRYDIQSCRVGQRGDYDQLSLEVWTDGTVRPEDAIAEAAKIIKEHFTVF
VNFDETALDLEDEPEEDDPAVLELLNTKIADVDFSVRARNCLLTMGIKTLGELTRISEQTLANTRNVGKKSLSEIQGKLQ
EYNLRLGMADYNHVGVVSRLMRQKEEIDEA

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=325, Percent_Identity=37.2307692307692, Blast_Score=187, Evalue=1e-48,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_TREPA (O83242)

Other databases:

- EMBL:   AE000520
- PIR:   B71352
- RefSeq:   NP_218652.1
- ProteinModelPortal:   O83242
- IntAct:   O83242
- GeneID:   2611469
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0212
- NMPDR:   fig|243276.1.peg.211
- TIGR:   TP_0212
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BioCyc:   TPAL243276:TP_0212-MONOMER
- BRENDA:   2.7.7.6
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 39545; Mature: 39414

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRRNLLKGFKRPKVLEFLSENSSECYGKFTASPFETGFGTTVGNCLRRVLLSSIQGYAV
CCCHHHHHHCCCCHHHHHHHCCCCHHCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEE
TGVRITSFDADGVAHFISSEFEQIPHVREDTLEILNNFKRLRFLLPQGAESSTFTYEFRG
EEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
AVSLTGKDFAKKFQLEVLSQDLLIMEMMDGAHVEVELHVEFGRGYVPAESHDRYADLVGV
EEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHH
IPVDAIFSPVLRVRYDIQSCRVGQRGDYDQLSLEVWTDGTVRPEDAIAEAAKIIKEHFTV
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCEE
FVNFDETALDLEDEPEEDDPAVLELLNTKIADVDFSVRARNCLLTMGIKTLGELTRISEQ
EEEECCCEECCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
TLANTRNVGKKSLSEIQGKLQEYNLRLGMADYNHVGVVSRLMRQKEEIDEA
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PRRNLLKGFKRPKVLEFLSENSSECYGKFTASPFETGFGTTVGNCLRRVLLSSIQGYAV
CCHHHHHHCCCCHHHHHHHCCCCHHCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEE
TGVRITSFDADGVAHFISSEFEQIPHVREDTLEILNNFKRLRFLLPQGAESSTFTYEFRG
EEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
AVSLTGKDFAKKFQLEVLSQDLLIMEMMDGAHVEVELHVEFGRGYVPAESHDRYADLVGV
EEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHH
IPVDAIFSPVLRVRYDIQSCRVGQRGDYDQLSLEVWTDGTVRPEDAIAEAAKIIKEHFTV
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCEE
FVNFDETALDLEDEPEEDDPAVLELLNTKIADVDFSVRARNCLLTMGIKTLGELTRISEQ
EEEECCCEECCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
TLANTRNVGKKSLSEIQGKLQEYNLRLGMADYNHVGVVSRLMRQKEEIDEA
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876