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The map label for this gene is tuf [H]

Identifier: 189025420

GI number: 189025420

Start: 204265

End: 205752

Strand: Direct

Name: tuf [H]

Synonym: TPASS_0187

Alternate gene names: 189025420

Gene position: 204265-205752 (Clockwise)

Preceding gene: 189025419

Following gene: 189025421

Centisome position: 17.93

GC content: 51.88

Gene sequence:

>1488_bases
TTGACAGCCTGCGTTTGTCTTATTACCATCGGCCGCAAAATACCGGACTTGCGCGCCCGCGGTGGGACGCAAGAGGATGC
CAAACCAGTCATCAAATGTTGGTTTGATGGTGTTCAGGTGGGTCCCCTTTCAAGCGTGCGCAATGCGCTTGGATCCCGCG
CTGGTTGTGCGCGGTGCCTGAGAGGCCCGTTTGGTTGTAGCGCTGTCGATCCTTCGTTTCGCTTTTTTGTGCCTCCTTGT
TGCGTGCGGGCGTGCGGGGTCCGCCGTATCTGTGCCACAGATCTTTTGAGGAGGATTTTCATGGCCAAGGAAAAGTTCGC
GCGCACTAAAGTTCACATGAACGTGGGTACTATTGGGCACGTCGATCACGGGAAGACAACGCTCTCTGCGGCGATCACCT
CGTACTGTGCAAAGAAGTTCGGTGATAAGCAACTAAAATACGACGAGATTGACAATGCGCCCGAAGAGAAAGCGCGCGGG
ATCACCATTAACACGCGTCATCTTGAGTATCAGTCCGATCGTCGTCATTACGCGCATATTGATTGTCCTGGGCACGCGGA
CTATGTGAAGAATATGATCACGGGTGCTGCGCAGATGGACGGTGGTATTCTCGTCGTGTCTGCGCCTGACGGCGTTATGC
CACAGACGAAGGAGCATCTTCTGCTCGCCCGTCAGGTTGGTGTTCCCTCCATCATTGTTTTTTTGAACAAGGTTGATTTG
GTTGATGATCCTGAGTTGCTAGAGCTGGTGGAAGAAGAGGTGCGTGATGCGCTTGCTGGATATGGGTTTTCGCGTGAGAC
GCCTATCGTCAAGGGGTCTGCGTTTAAAGCTCTGCAGGATGGCGCTTCCCCGGAGGATGCAGCTTGTATTGAGGAACTGC
TTGCGGCCATGGATTCCTACTTTGAAGACCCAGTGCGTGACGACGCAAGACCTTTCTTGCTCTCTATCGAGGATGTGTAC
ACTATTTCTGGGCGTGGTACCGTTGTCACGGGGCGCATCGAATGTGGGGTAATTAGTCTGAATGAAGAGGTCGAGATCGT
CGGGATTAAGCCCACTAAGAAAACAGTGGTTACTGGCATTGAGATGTTTAATAAGTTGCTTGATCAGGGAATTGCAGGTG
ATAACGTGGGGCTGCTTTTGCGCGGGGTGGATAAAAAAGAGGTTGAGCGCGGTCAGGTGCTTTCTAAGCCCGGTTCTATT
AAGCCACACACCAAGTTTGAGGCGCAGATCTACGTGCTCTCTAAGGAAGAGGGTGGCCGTCACAGTCCTTTTTTTCAAGG
TTATCGTCCGCAGTTTTATTTTAGAACTACTGACATTACCGGTACGATTTCTCTTCCTGAAGGGGTAGACATGGTGAAGC
CGGGGGATAACACCAAGATTATAGGTGAGCTCATCCACCCGATAGCTATGGACAAGGGTCTGAAGCTTGCGATTCGTGAA
GGGGGGCGCACTATTGCTTCTGGTCAGGTGACAGAGATTTTGTTGTAG

Upstream 100 bases:

>100_bases
TTTGCGAGAGGCGTTTGCAGAACTTGCGCGCACGTGAGTGGTCGTGAGAATGGGTACGCCGTGTACTATTTGAATTCGGG
TATATTTCCTTTGGAAGAGC

Downstream 100 bases:

>100_bases
GCGTTTGCGGCGCGGAGTGTGTTTGGAGTTATTTTGCAAGGTGGGTGCGGTTTTAGGCTGATGGAGGGGTTATGGCCAGG
GAGAGAATTCGGGTAAAACT

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MTACVCLITIGRKIPDLRARGGTQEDAKPVIKCWFDGVQVGPLSSVRNALGSRAGCARCLRGPFGCSAVDPSFRFFVPPC
CVRACGVRRICATDLLRRIFMAKEKFARTKVHMNVGTIGHVDHGKTTLSAAITSYCAKKFGDKQLKYDEIDNAPEEKARG
ITINTRHLEYQSDRRHYAHIDCPGHADYVKNMITGAAQMDGGILVVSAPDGVMPQTKEHLLLARQVGVPSIIVFLNKVDL
VDDPELLELVEEEVRDALAGYGFSRETPIVKGSAFKALQDGASPEDAACIEELLAAMDSYFEDPVRDDARPFLLSIEDVY
TISGRGTVVTGRIECGVISLNEEVEIVGIKPTKKTVVTGIEMFNKLLDQGIAGDNVGLLLRGVDKKEVERGQVLSKPGSI
KPHTKFEAQIYVLSKEEGGRHSPFFQGYRPQFYFRTTDITGTISLPEGVDMVKPGDNTKIIGELIHPIAMDKGLKLAIRE
GGRTIASGQVTEILL

Sequences:

>Translated_495_residues
MTACVCLITIGRKIPDLRARGGTQEDAKPVIKCWFDGVQVGPLSSVRNALGSRAGCARCLRGPFGCSAVDPSFRFFVPPC
CVRACGVRRICATDLLRRIFMAKEKFARTKVHMNVGTIGHVDHGKTTLSAAITSYCAKKFGDKQLKYDEIDNAPEEKARG
ITINTRHLEYQSDRRHYAHIDCPGHADYVKNMITGAAQMDGGILVVSAPDGVMPQTKEHLLLARQVGVPSIIVFLNKVDL
VDDPELLELVEEEVRDALAGYGFSRETPIVKGSAFKALQDGASPEDAACIEELLAAMDSYFEDPVRDDARPFLLSIEDVY
TISGRGTVVTGRIECGVISLNEEVEIVGIKPTKKTVVTGIEMFNKLLDQGIAGDNVGLLLRGVDKKEVERGQVLSKPGSI
KPHTKFEAQIYVLSKEEGGRHSPFFQGYRPQFYFRTTDITGTISLPEGVDMVKPGDNTKIIGELIHPIAMDKGLKLAIRE
GGRTIASGQVTEILL
>Mature_494_residues
TACVCLITIGRKIPDLRARGGTQEDAKPVIKCWFDGVQVGPLSSVRNALGSRAGCARCLRGPFGCSAVDPSFRFFVPPCC
VRACGVRRICATDLLRRIFMAKEKFARTKVHMNVGTIGHVDHGKTTLSAAITSYCAKKFGDKQLKYDEIDNAPEEKARGI
TINTRHLEYQSDRRHYAHIDCPGHADYVKNMITGAAQMDGGILVVSAPDGVMPQTKEHLLLARQVGVPSIIVFLNKVDLV
DDPELLELVEEEVRDALAGYGFSRETPIVKGSAFKALQDGASPEDAACIEELLAAMDSYFEDPVRDDARPFLLSIEDVYT
ISGRGTVVTGRIECGVISLNEEVEIVGIKPTKKTVVTGIEMFNKLLDQGIAGDNVGLLLRGVDKKEVERGQVLSKPGSIK
PHTKFEAQIYVLSKEEGGRHSPFFQGYRPQFYFRTTDITGTISLPEGVDMVKPGDNTKIIGELIHPIAMDKGLKLAIREG
GRTIASGQVTEILL

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=53.9240506329114, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI4503471, Length=351, Percent_Identity=31.6239316239316, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI4503475, Length=353, Percent_Identity=30.8781869688385, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI223555963, Length=440, Percent_Identity=29.5454545454545, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI5729864, Length=440, Percent_Identity=29.5454545454545, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI54607086, Length=350, Percent_Identity=29.7142857142857, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI46094014, Length=380, Percent_Identity=28.6842105263158, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI194018522, Length=345, Percent_Identity=28.9855072463768, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI194018520, Length=345, Percent_Identity=28.9855072463768, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI194097354, Length=345, Percent_Identity=28.9855072463768, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI157426893, Length=221, Percent_Identity=31.2217194570136, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=35.5555555555556, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=69.3670886075949, Blast_Score=537, Evalue=1e-154,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=69.3670886075949, Blast_Score=537, Evalue=1e-154,
Organism=Escherichia coli, GI2367247, Length=344, Percent_Identity=31.6860465116279, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1789108, Length=305, Percent_Identity=25.5737704918033, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI48994988, Length=239, Percent_Identity=30.9623430962343, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1788922, Length=149, Percent_Identity=36.241610738255, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17556456, Length=400, Percent_Identity=48.75, Blast_Score=351, Evalue=6e-97,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=42.8940568475452, Blast_Score=310, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI17552884, Length=359, Percent_Identity=32.033426183844, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17569207, Length=359, Percent_Identity=32.033426183844, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI32566303, Length=328, Percent_Identity=31.0975609756098, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17506081, Length=272, Percent_Identity=31.6176470588235, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI115532067, Length=436, Percent_Identity=28.2110091743119, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI115532065, Length=436, Percent_Identity=28.2110091743119, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI32566629, Length=357, Percent_Identity=26.6106442577031, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI32566301, Length=149, Percent_Identity=34.2281879194631, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=128, Percent_Identity=35.15625, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=57.8282828282828, Blast_Score=457, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6325337, Length=350, Percent_Identity=33.1428571428571, Blast_Score=153, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=350, Percent_Identity=33.1428571428571, Blast_Score=153, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6320377, Length=342, Percent_Identity=29.5321637426901, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6322937, Length=451, Percent_Identity=24.6119733924612, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323320, Length=312, Percent_Identity=26.6025641025641, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281363316, Length=400, Percent_Identity=52.25, Blast_Score=422, Evalue=1e-118,
Organism=Drosophila melanogaster, GI17864358, Length=400, Percent_Identity=52.25, Blast_Score=422, Evalue=1e-118,
Organism=Drosophila melanogaster, GI19921738, Length=384, Percent_Identity=49.21875, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI45553807, Length=357, Percent_Identity=30.5322128851541, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI45553816, Length=357, Percent_Identity=30.5322128851541, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24651721, Length=357, Percent_Identity=30.5322128851541, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI17864154, Length=357, Percent_Identity=30.5322128851541, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24652838, Length=353, Percent_Identity=31.4447592067989, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI17137572, Length=353, Percent_Identity=31.4447592067989, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=29.5081967213115, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI45550900, Length=446, Percent_Identity=28.6995515695067, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17137380, Length=354, Percent_Identity=28.2485875706215, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24647048, Length=456, Percent_Identity=24.1228070175439, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24647046, Length=454, Percent_Identity=24.0088105726872, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 54076; Mature: 53945

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTACVCLITIGRKIPDLRARGGTQEDAKPVIKCWFDGVQVGPLSSVRNALGSRAGCARCL
CCCEEEEEECCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHH
RGPFGCSAVDPSFRFFVPPCCVRACGVRRICATDLLRRIFMAKEKFARTKVHMNVGTIGH
CCCCCCCCCCCCCEEECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEC
VDHGKTTLSAAITSYCAKKFGDKQLKYDEIDNAPEEKARGITINTRHLEYQSDRRHYAHI
CCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHCCEEEEEEEECCCCCCCEEEEE
DCPGHADYVKNMITGAAQMDGGILVVSAPDGVMPQTKEHLLLARQVGVPSIIVFLNKVDL
ECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCC
VDDPELLELVEEEVRDALAGYGFSRETPIVKGSAFKALQDGASPEDAACIEELLAAMDSY
CCCHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHH
FEDPVRDDARPFLLSIEDVYTISGRGTVVTGRIECGVISLNEEVEIVGIKPTKKTVVTGI
HCCCCCCCCCCEEEEEEEEEEECCCCEEEEEEEEEEEEEECCCEEEEEECCCHHHHHHHH
EMFNKLLDQGIAGDNVGLLLRGVDKKEVERGQVLSKPGSIKPHTKFEAQIYVLSKEEGGR
HHHHHHHHCCCCCCCCEEEEECCCHHHHHHCHHHCCCCCCCCCCCEEEEEEEEECCCCCC
HSPFFQGYRPQFYFRTTDITGTISLPEGVDMVKPGDNTKIIGELIHPIAMDKGLKLAIRE
CCCCCCCCCCEEEEEEECCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEC
GGRTIASGQVTEILL
CCCEEECCCCCEECC
>Mature Secondary Structure 
TACVCLITIGRKIPDLRARGGTQEDAKPVIKCWFDGVQVGPLSSVRNALGSRAGCARCL
CCEEEEEECCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHH
RGPFGCSAVDPSFRFFVPPCCVRACGVRRICATDLLRRIFMAKEKFARTKVHMNVGTIGH
CCCCCCCCCCCCCEEECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEC
VDHGKTTLSAAITSYCAKKFGDKQLKYDEIDNAPEEKARGITINTRHLEYQSDRRHYAHI
CCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHCCEEEEEEEECCCCCCCEEEEE
DCPGHADYVKNMITGAAQMDGGILVVSAPDGVMPQTKEHLLLARQVGVPSIIVFLNKVDL
ECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCC
VDDPELLELVEEEVRDALAGYGFSRETPIVKGSAFKALQDGASPEDAACIEELLAAMDSY
CCCHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHH
FEDPVRDDARPFLLSIEDVYTISGRGTVVTGRIECGVISLNEEVEIVGIKPTKKTVVTGI
HCCCCCCCCCCEEEEEEEEEEECCCCEEEEEEEEEEEEEECCCEEEEEECCCHHHHHHHH
EMFNKLLDQGIAGDNVGLLLRGVDKKEVERGQVLSKPGSIKPHTKFEAQIYVLSKEEGGR
HHHHHHHHCCCCCCCCEEEEECCCHHHHHHCHHHCCCCCCCCCCCEEEEEEEEECCCCCC
HSPFFQGYRPQFYFRTTDITGTISLPEGVDMVKPGDNTKIIGELIHPIAMDKGLKLAIRE
CCCCCCCCCCEEEEEEECCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEC
GGRTIASGQVTEILL
CCCEEECCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]