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The map label for this gene is rnfC [H]

Identifier: 189025385

GI number: 189025385

Start: 174933

End: 176477

Strand: Reverse

Name: rnfC [H]

Synonym: TPASS_0152

Alternate gene names: 189025385

Gene position: 176477-174933 (Counterclockwise)

Preceding gene: 189025386

Following gene: 189025384

Centisome position: 15.49

GC content: 57.41

Gene sequence:

>1545_bases
GTGTGTCCCTGCCGCGTCGGGAGGCGCAGACCTTTTCTGTACCGTACAGAGGGCACACCAATGATAGAGCGCCTACGGAG
CAGTCGCGGGAAACTCACCCTCACCCACCAGATTTTCCCCCTCAGCTTTGGGGGGAATGCTTTTTTGCCTGCGCGCGCGC
TCGTTCCGTTCTCCGTTGATGCTGGAGAGCCAGCCGCCGTCGCTGTGGTAAAGGTTGGGGATACGGTCCGAGAAGGTCAG
CTGATCGCACGCGCCGCGCACGCCGGTGCTGCTCACGCACATGCCTCCGTCCCCGGTGTCGTCACCCGCTTGGTAAGTGC
TAATTTTCTCGCCGGTAGTGCCCTGCGCGCTGTCGAGATTCGTACACGCGGTTCCTTCGAACATCTTGGCAAGGTCCAAC
CAAATCGCCCGTGGCAGCACAGCACCGCTTCAGAATTGCTGCGCCTAGTTACAGATGCAGGAGTAGTGGCCACACGCCTA
CATCCGCACGCCCAGATCACGAGCACCGCAACGGGCACGCACGCGGGTGCACAGCACACGTACGCGAAAGACTACGGACA
GAAGAGAAGGGCTGAAGCGCACACGCTGCGTCTCATGCGCGCGGCGTGGGAAAGCGGCAATGCGCTCGCCACGCACCTCC
ACCTGCACGTGCGTAAGGGTGTACGGAAACTTACGCTCTACCTTTGTGACGACGACGCTACCTGCCCTTTGAGTTCGTTC
CTTGCGCAGGAGTTTCCAGAACCTGTTGCTACCGGTACCGCCATTATTGCACGGATACTGGACGCTACGTATACCGCGTG
TCTCCACACGCTGCCAAAACGCTCCCCCGGTCTTGCAAGGATGCGCGCTGTCTTTCCATTCAACGAGATGCACGACGCGT
ATAGACGACATTATCCTTTTAGCAATCTATGTGCCCCACGCTATCGTGCAGGTTGCACAATCGATGCACTCACTGCAGTG
CACGTGTATGAGGCAGTGGTACTCAGTCAGCCGCAAATCAGTTCCTACATTGCTCTGACAGGCGCTGGATTAAAATCACC
GCAGGTACTCCGCGCGCGTATCGGCACCCCCCTTGGCGCGCTCATCGAGGAGTGTGGAGGGTTTCGCACACGCCCCGGGC
ATCTCATCATCAATGGACTGCTCAAGGGTAGTGTTTTAGAGTCGTTGGACCTGCCTTTCTCAAAGGGGATCAAATCGCTC
CACGTCACCGGTAAAGCGCTTTCAAGCTCTGCGTCCTGTACCTCCTGTCAAAACTGTGGTGATTGCGCGCGCATTTGCCC
AGTATATCTTGACCCAATAAAAATTGCGCGTGCCGCACACCGTAATCAGTTTACTGAAGAAGTGCTCCAATCCCTGCGGA
TTTGCCACCAATGCGGTCTGTGTTCTGCCGCCTGTACTGCGCGTATTCCTCTTGCAAAACTTTTGCACGATGCACAAGAA
CGCGCACTGCATCTTTCCCGTGCTCCAGTCACCAAAATAGAACCCCACTCCACACAAAGCGTCGGGAAAACTATCCGCGA
GGCACCTGCCAATGCGCACCGCTGA

Upstream 100 bases:

>100_bases
GTTCTACAGCTCCATGAGCCCTTGAGTCTCCGGTGGACGTGCATGCAATGCGGCGGACCCCTCCACACAGAGGAAGAGGC
GGTGCTGTGCGCGTTCTCTC

Downstream 100 bases:

>100_bases
GTACAAACACGCACCCTTCCTTTACACCGGCTTAAGTGCTGGACAGAACAACAGTGTACTGTTGGCGCTGCTTGTTGCGC
ACGTGTTCGTCGTTGCAGCC

Product: nitrogen fixation protein

Products: NA

Alternate protein names: Nitrogen fixation protein rnfC [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MCPCRVGRRRPFLYRTEGTPMIERLRSSRGKLTLTHQIFPLSFGGNAFLPARALVPFSVDAGEPAAVAVVKVGDTVREGQ
LIARAAHAGAAHAHASVPGVVTRLVSANFLAGSALRAVEIRTRGSFEHLGKVQPNRPWQHSTASELLRLVTDAGVVATRL
HPHAQITSTATGTHAGAQHTYAKDYGQKRRAEAHTLRLMRAAWESGNALATHLHLHVRKGVRKLTLYLCDDDATCPLSSF
LAQEFPEPVATGTAIIARILDATYTACLHTLPKRSPGLARMRAVFPFNEMHDAYRRHYPFSNLCAPRYRAGCTIDALTAV
HVYEAVVLSQPQISSYIALTGAGLKSPQVLRARIGTPLGALIEECGGFRTRPGHLIINGLLKGSVLESLDLPFSKGIKSL
HVTGKALSSSASCTSCQNCGDCARICPVYLDPIKIARAAHRNQFTEEVLQSLRICHQCGLCSAACTARIPLAKLLHDAQE
RALHLSRAPVTKIEPHSTQSVGKTIREAPANAHR

Sequences:

>Translated_514_residues
MCPCRVGRRRPFLYRTEGTPMIERLRSSRGKLTLTHQIFPLSFGGNAFLPARALVPFSVDAGEPAAVAVVKVGDTVREGQ
LIARAAHAGAAHAHASVPGVVTRLVSANFLAGSALRAVEIRTRGSFEHLGKVQPNRPWQHSTASELLRLVTDAGVVATRL
HPHAQITSTATGTHAGAQHTYAKDYGQKRRAEAHTLRLMRAAWESGNALATHLHLHVRKGVRKLTLYLCDDDATCPLSSF
LAQEFPEPVATGTAIIARILDATYTACLHTLPKRSPGLARMRAVFPFNEMHDAYRRHYPFSNLCAPRYRAGCTIDALTAV
HVYEAVVLSQPQISSYIALTGAGLKSPQVLRARIGTPLGALIEECGGFRTRPGHLIINGLLKGSVLESLDLPFSKGIKSL
HVTGKALSSSASCTSCQNCGDCARICPVYLDPIKIARAAHRNQFTEEVLQSLRICHQCGLCSAACTARIPLAKLLHDAQE
RALHLSRAPVTKIEPHSTQSVGKTIREAPANAHR
>Mature_514_residues
MCPCRVGRRRPFLYRTEGTPMIERLRSSRGKLTLTHQIFPLSFGGNAFLPARALVPFSVDAGEPAAVAVVKVGDTVREGQ
LIARAAHAGAAHAHASVPGVVTRLVSANFLAGSALRAVEIRTRGSFEHLGKVQPNRPWQHSTASELLRLVTDAGVVATRL
HPHAQITSTATGTHAGAQHTYAKDYGQKRRAEAHTLRLMRAAWESGNALATHLHLHVRKGVRKLTLYLCDDDATCPLSSF
LAQEFPEPVATGTAIIARILDATYTACLHTLPKRSPGLARMRAVFPFNEMHDAYRRHYPFSNLCAPRYRAGCTIDALTAV
HVYEAVVLSQPQISSYIALTGAGLKSPQVLRARIGTPLGALIEECGGFRTRPGHLIINGLLKGSVLESLDLPFSKGIKSL
HVTGKALSSSASCTSCQNCGDCARICPVYLDPIKIARAAHRNQFTEEVLQSLRICHQCGLCSAACTARIPLAKLLHDAQE
RALHLSRAPVTKIEPHSTQSVGKTIREAPANAHR

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase. Stabilizes rnfB [H]

COG id: COG4656

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfC

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Probable) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787916, Length=166, Percent_Identity=29.5180722891566, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR010208
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10531 SLBB [H]

EC number: NA

Molecular weight: Translated: 55618; Mature: 55618

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCPCRVGRRRPFLYRTEGTPMIERLRSSRGKLTLTHQIFPLSFGGNAFLPARALVPFSVD
CCCCCCCCCCCEEEECCCCHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCEECEEECC
AGEPAAVAVVKVGDTVREGQLIARAAHAGAAHAHASVPGVVTRLVSANFLAGSALRAVEI
CCCCCEEEEEEECCCHHHCHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHCCCCEEEEEE
RTRGSFEHLGKVQPNRPWQHSTASELLRLVTDAGVVATRLHPHAQITSTATGTHAGAQHT
ECCCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHEEECCCCCEEECCCCCCCCCCCHH
YAKDYGQKRRAEAHTLRLMRAAWESGNALATHLHLHVRKGVRKLTLYLCDDDATCPLSSF
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCEEEEEEEECCCCCCCHHHH
LAQEFPEPVATGTAIIARILDATYTACLHTLPKRSPGLARMRAVFPFNEMHDAYRRHYPF
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHCCCC
SNLCAPRYRAGCTIDALTAVHVYEAVVLSQPQISSYIALTGAGLKSPQVLRARIGTPLGA
HHCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCHHCEEEEECCCCCCHHHHHHHHCCCHHH
LIEECGGFRTRPGHLIINGLLKGSVLESLDLPFSKGIKSLHVTGKALSSSASCTSCQNCG
HHHHHCCCCCCCCCEEEEHHHHHHHHHHCCCCHHHCCCEEEEEHHHHCCCCCCCCCCCCC
DCARICPVYLDPIKIARAAHRNQFTEEVLQSLRICHQCGLCSAACTARIPLAKLLHDAQE
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHH
RALHLSRAPVTKIEPHSTQSVGKTIREAPANAHR
HHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MCPCRVGRRRPFLYRTEGTPMIERLRSSRGKLTLTHQIFPLSFGGNAFLPARALVPFSVD
CCCCCCCCCCCEEEECCCCHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCEECEEECC
AGEPAAVAVVKVGDTVREGQLIARAAHAGAAHAHASVPGVVTRLVSANFLAGSALRAVEI
CCCCCEEEEEEECCCHHHCHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHCCCCEEEEEE
RTRGSFEHLGKVQPNRPWQHSTASELLRLVTDAGVVATRLHPHAQITSTATGTHAGAQHT
ECCCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHEEECCCCCEEECCCCCCCCCCCHH
YAKDYGQKRRAEAHTLRLMRAAWESGNALATHLHLHVRKGVRKLTLYLCDDDATCPLSSF
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCEEEEEEEECCCCCCCHHHH
LAQEFPEPVATGTAIIARILDATYTACLHTLPKRSPGLARMRAVFPFNEMHDAYRRHYPF
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHCCCC
SNLCAPRYRAGCTIDALTAVHVYEAVVLSQPQISSYIALTGAGLKSPQVLRARIGTPLGA
HHCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCHHCEEEEECCCCCCHHHHHHHHCCCHHH
LIEECGGFRTRPGHLIINGLLKGSVLESLDLPFSKGIKSLHVTGKALSSSASCTSCQNCG
HHHHHCCCCCCCCCEEEEHHHHHHHHHHCCCCHHHCCCEEEEEHHHHCCCCCCCCCCCCC
DCARICPVYLDPIKIARAAHRNQFTEEVLQSLRICHQCGLCSAACTARIPLAKLLHDAQE
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHH
RALHLSRAPVTKIEPHSTQSVGKTIREAPANAHR
HHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 4Fe-4S clusters [C]

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8264535; 9492268; 9154934 [H]