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The map label for this gene is pckA

Identifier: 189025356

GI number: 189025356

Start: 141022

End: 142878

Strand: Reverse

Name: pckA

Synonym: TPASS_0122

Alternate gene names: 189025356

Gene position: 142878-141022 (Counterclockwise)

Preceding gene: 189025360

Following gene: 189025354

Centisome position: 12.54

GC content: 53.96

Gene sequence:

>1857_bases
ATGGAACTTCACGAGATTGCCCACGCTCGAGCAAAGGCGTGGATAGAAGAGATGGTCGCACTCTGTGCCCCAGATACCGT
CTACGTCTGCGACGGTTCGAAAAAAGAGTACGACACCATTATGCAGAAAATGGTGGATGCTGGCTTGGCTACCCCATTAA
AAAAACGTAAGAACTGCTTCCTCTTTCGCTCACAGCCCTCCGACGTTGCGCGCGTCGAAGCGCGCACCTTCATCGCCTCA
AAAAGGGAGGATGATGCAGGACCCACTAACCATTGGACCGATCCTGCAGAATTAAAAAAGACTATGACCGGTCTCTATTC
CCAGTGCATGAAGGGCCGTACCATGTACGTCATTCCCTTTTCCATGGGACCGGTCGGCTCACCCATCTCTAAAAATGGCA
TCGAAATCACCGATTCTGAGTACGTCGTTTGCAACATGCACATCATGACACGCGTGGGTACCCGCGTGCTTGAGGCGTTG
GGTACTGATGGTGAGTTCGTCCCCTGTTTGCACTCTGTGGGGAAGCCGTTAGGACCGGGCGTAACTGACGCAGGCCAGTG
GCCCTGCGCGGATATGGAGCGCAAATACATCTCTCACTTTCCTGAAGAGCGCTTGGTTTGGTCTTTCGGTTCAGGATACG
GAGGGAATGCCCTGTTGGGGAAAAAGTGTTTCGCATTACGCATCGCTTCAGTACTCGCACGAGACGAAGGGTGGCTTGCA
GAGCACATGCTCATCCTTAAAATCACCAACCCTGCTGGAAAGACCAAGTACATTGGTGCGGCGTTTCCCTCTGCATGTGG
CAAAACGAATCTGGCTATGATGATCCCTACCCTTCCTGGATGGAAGGTCGAGACCGTAGGCGATGACATCGCGTGGATGA
AATTCGGAAAGGACGGCCGCCTGTACGCTATCAATCCTGAAGCGGGGTTCTTCGGCGTAGCACCAGGGACATCTGATTTC
TCGAACAAGAATGCCATGGACTCTATCAAGGAAAACGCCATTTTCACCAACTGTGGTCTGACCGAAGACGGCGACGTGTG
GTGGGAGGGTATCGGCTATCCCGCTAAAGGAACAATCATTGATTGGCACGGCGTGTCGCGCCCCGCACCTACACGCGATA
AAAGTCCAAAAGGAGAAGAAATTGCGCATCCGAATGCGCGTTTTACCGCTCCTGCGCGACAGTGCCCTGCAATTGCAAGT
AACTGGGAAGATCCCGAAGGGGTCCCTATCGACGCTTTTCTGTTTGGCGGCCGTCGCCCCAGTACGGTTCCCCTGGTGCA
TCAGGCCCGGGACTGGAATCACGGGGTGTTTCTAGGTTCCATCATCGGCTCGGAGGTAACTGCAGCAGTTATCTCTGACC
AGGTAGGTCAGATTCGCCGAGACCCGTTCGCGATGCTCCCCTTCTGCGGCTACCATATGGCAGACTACTTTTCGCACTGG
ATTAAGCTCGGATCGCAGGCACGTGCCGAAAACCTGCCAAAGATTTTCTGCGTTAACTGGTTTCGTAAAGATGCAGAGGG
CAACTTCCTGTGGCCCGGATATGGCGATAACAGCCGAGTACTCGCGTGGATCTTTGACCGCTGTGATGGTGTGGACAACG
CGGTGGAAACAGCGATCGGTTGGATGCCCAAAGAGGGAGCCTTGAACACAGAGGGGTTAAACGTAAGCACGCAGGCAGTG
AAAGAGCTTTTGTCGGTGGACATAGCCGGATGGAAAAAGGAAATTAAAGACATCCGAGAGAATCACTACCCAAAGTTTGG
CGCGCGGCTGCCACAGCAGCTTCGCGATGCACTCGAGGTTTTGGAAGCTCGCATCAACGGCAGCGAGGGGGCTGCGTGCA
CGCGTGATATGTGTTAG

Upstream 100 bases:

>100_bases
TTGAAGAGGTCTTTTTTATTTACCTTTAGCGCGCTATACTTCTGGGGCGATACGTGCCTGGAATACCCGTGCAGCACGCT
ACGCACCAAGGAGCGGCGTT

Downstream 100 bases:

>100_bases
CCGGTAGAGAGCGCGTCCTCTTTTCTCACCGTTCTGCGGCGCTGCGTGGGCGCGCGGGGCAGAAGAGGACCCCTCGGGGG
GGGGCGGCGGTGCATCACTG

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 618; Mature: 618

Protein sequence:

>618_residues
MELHEIAHARAKAWIEEMVALCAPDTVYVCDGSKKEYDTIMQKMVDAGLATPLKKRKNCFLFRSQPSDVARVEARTFIAS
KREDDAGPTNHWTDPAELKKTMTGLYSQCMKGRTMYVIPFSMGPVGSPISKNGIEITDSEYVVCNMHIMTRVGTRVLEAL
GTDGEFVPCLHSVGKPLGPGVTDAGQWPCADMERKYISHFPEERLVWSFGSGYGGNALLGKKCFALRIASVLARDEGWLA
EHMLILKITNPAGKTKYIGAAFPSACGKTNLAMMIPTLPGWKVETVGDDIAWMKFGKDGRLYAINPEAGFFGVAPGTSDF
SNKNAMDSIKENAIFTNCGLTEDGDVWWEGIGYPAKGTIIDWHGVSRPAPTRDKSPKGEEIAHPNARFTAPARQCPAIAS
NWEDPEGVPIDAFLFGGRRPSTVPLVHQARDWNHGVFLGSIIGSEVTAAVISDQVGQIRRDPFAMLPFCGYHMADYFSHW
IKLGSQARAENLPKIFCVNWFRKDAEGNFLWPGYGDNSRVLAWIFDRCDGVDNAVETAIGWMPKEGALNTEGLNVSTQAV
KELLSVDIAGWKKEIKDIRENHYPKFGARLPQQLRDALEVLEARINGSEGAACTRDMC

Sequences:

>Translated_618_residues
MELHEIAHARAKAWIEEMVALCAPDTVYVCDGSKKEYDTIMQKMVDAGLATPLKKRKNCFLFRSQPSDVARVEARTFIAS
KREDDAGPTNHWTDPAELKKTMTGLYSQCMKGRTMYVIPFSMGPVGSPISKNGIEITDSEYVVCNMHIMTRVGTRVLEAL
GTDGEFVPCLHSVGKPLGPGVTDAGQWPCADMERKYISHFPEERLVWSFGSGYGGNALLGKKCFALRIASVLARDEGWLA
EHMLILKITNPAGKTKYIGAAFPSACGKTNLAMMIPTLPGWKVETVGDDIAWMKFGKDGRLYAINPEAGFFGVAPGTSDF
SNKNAMDSIKENAIFTNCGLTEDGDVWWEGIGYPAKGTIIDWHGVSRPAPTRDKSPKGEEIAHPNARFTAPARQCPAIAS
NWEDPEGVPIDAFLFGGRRPSTVPLVHQARDWNHGVFLGSIIGSEVTAAVISDQVGQIRRDPFAMLPFCGYHMADYFSHW
IKLGSQARAENLPKIFCVNWFRKDAEGNFLWPGYGDNSRVLAWIFDRCDGVDNAVETAIGWMPKEGALNTEGLNVSTQAV
KELLSVDIAGWKKEIKDIRENHYPKFGARLPQQLRDALEVLEARINGSEGAACTRDMC
>Mature_618_residues
MELHEIAHARAKAWIEEMVALCAPDTVYVCDGSKKEYDTIMQKMVDAGLATPLKKRKNCFLFRSQPSDVARVEARTFIAS
KREDDAGPTNHWTDPAELKKTMTGLYSQCMKGRTMYVIPFSMGPVGSPISKNGIEITDSEYVVCNMHIMTRVGTRVLEAL
GTDGEFVPCLHSVGKPLGPGVTDAGQWPCADMERKYISHFPEERLVWSFGSGYGGNALLGKKCFALRIASVLARDEGWLA
EHMLILKITNPAGKTKYIGAAFPSACGKTNLAMMIPTLPGWKVETVGDDIAWMKFGKDGRLYAINPEAGFFGVAPGTSDF
SNKNAMDSIKENAIFTNCGLTEDGDVWWEGIGYPAKGTIIDWHGVSRPAPTRDKSPKGEEIAHPNARFTAPARQCPAIAS
NWEDPEGVPIDAFLFGGRRPSTVPLVHQARDWNHGVFLGSIIGSEVTAAVISDQVGQIRRDPFAMLPFCGYHMADYFSHW
IKLGSQARAENLPKIFCVNWFRKDAEGNFLWPGYGDNSRVLAWIFDRCDGVDNAVETAIGWMPKEGALNTEGLNVSTQAV
KELLSVDIAGWKKEIKDIRENHYPKFGARLPQQLRDALEVLEARINGSEGAACTRDMC

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family

Homologues:

Organism=Homo sapiens, GI66346721, Length=607, Percent_Identity=53.2125205930807, Blast_Score=629, Evalue=1e-180,
Organism=Homo sapiens, GI187281517, Length=607, Percent_Identity=53.2125205930807, Blast_Score=622, Evalue=1e-178,
Organism=Homo sapiens, GI66346723, Length=384, Percent_Identity=54.6875, Blast_Score=415, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17555492, Length=623, Percent_Identity=50.2407704654896, Blast_Score=598, Evalue=1e-171,
Organism=Caenorhabditis elegans, GI71989645, Length=614, Percent_Identity=49.0228013029316, Blast_Score=583, Evalue=1e-166,
Organism=Caenorhabditis elegans, GI71989658, Length=614, Percent_Identity=49.0228013029316, Blast_Score=582, Evalue=1e-166,
Organism=Caenorhabditis elegans, GI71989653, Length=599, Percent_Identity=49.5826377295492, Blast_Score=575, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI32564411, Length=558, Percent_Identity=51.4336917562724, Blast_Score=559, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI32564407, Length=531, Percent_Identity=52.3540489642185, Blast_Score=535, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI71989649, Length=513, Percent_Identity=49.7076023391813, Blast_Score=496, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI32564409, Length=376, Percent_Identity=50, Blast_Score=355, Evalue=5e-98,
Organism=Caenorhabditis elegans, GI17540956, Length=219, Percent_Identity=31.0502283105023, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24655085, Length=607, Percent_Identity=53.2125205930807, Blast_Score=629, Evalue=1e-180,
Organism=Drosophila melanogaster, GI24655082, Length=615, Percent_Identity=51.3821138211382, Blast_Score=591, Evalue=1e-169,
Organism=Drosophila melanogaster, GI161077226, Length=575, Percent_Identity=52.695652173913, Blast_Score=564, Evalue=1e-161,
Organism=Drosophila melanogaster, GI24655088, Length=504, Percent_Identity=56.3492063492063, Blast_Score=545, Evalue=1e-155,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PCKG_TREPA (O83159)

Other databases:

- EMBL:   AE000520
- PIR:   A71364
- RefSeq:   NP_218562.1
- ProteinModelPortal:   O83159
- SMR:   O83159
- GeneID:   2610930
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0122
- NMPDR:   fig|243276.1.peg.121
- TIGR:   TP_0122
- HOGENOM:   HBG484437
- OMA:   CGYHMGD
- ProtClustDB:   PRK04210
- BioCyc:   TPAL243276:TP_0122-MONOMER
- BRENDA:   4.1.1.32
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00452
- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PANTHER:   PTHR11561
- PIRSF:   PIRSF001348

Pfam domain/function: PF00821 PEPCK; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.32

Molecular weight: Translated: 68125; Mature: 68125

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00505 PEPCK_GTP

Important sites: ACT_SITE 266-266 BINDING 71-71 BINDING 215-215 BINDING 222-222 BINDING 264-264 BINDING 387-387 BINDING 418-418

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELHEIAHARAKAWIEEMVALCAPDTVYVCDGSKKEYDTIMQKMVDAGLATPLKKRKNCF
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCHHHHCCCEE
LFRSQPSDVARVEARTFIASKREDDAGPTNHWTDPAELKKTMTGLYSQCMKGRTMYVIPF
EEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEE
SMGPVGSPISKNGIEITDSEYVVCNMHIMTRVGTRVLEALGTDGEFVPCLHSVGKPLGPG
CCCCCCCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCC
VTDAGQWPCADMERKYISHFPEERLVWSFGSGYGGNALLGKKCFALRIASVLARDEGWLA
CCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHH
EHMLILKITNPAGKTKYIGAAFPSACGKTNLAMMIPTLPGWKVETVGDDIAWMKFGKDGR
HCEEEEEEECCCCCCEEECCCCCHHCCCCCEEEEEECCCCCEEEECCCCEEEEEECCCCC
LYAINPEAGFFGVAPGTSDFSNKNAMDSIKENAIFTNCGLTEDGDVWWEGIGYPAKGTII
EEEECCCCCEEECCCCCCCCCCCHHHHHHHHCCEEEECCCCCCCCEEEECCCCCCCCCEE
DWHGVSRPAPTRDKSPKGEEIAHPNARFTAPARQCPAIASNWEDPEGVPIDAFLFGGRRP
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHCCCCCCCCCEEEEEEECCCCC
STVPLVHQARDWNHGVFLGSIIGSEVTAAVISDQVGQIRRDPFAMLPFCGYHMADYFSHW
CCCCHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
IKLGSQARAENLPKIFCVNWFRKDAEGNFLWPGYGDNSRVLAWIFDRCDGVDNAVETAIG
HHCCCHHHHCCCCEEEEEHHHHHCCCCCEECCCCCCCCCEEEEEEHHCCCCCHHHHHHHC
WMPKEGALNTEGLNVSTQAVKELLSVDIAGWKKEIKDIRENHYPKFGARLPQQLRDALEV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LEARINGSEGAACTRDMC
HHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MELHEIAHARAKAWIEEMVALCAPDTVYVCDGSKKEYDTIMQKMVDAGLATPLKKRKNCF
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCHHHHCCCEE
LFRSQPSDVARVEARTFIASKREDDAGPTNHWTDPAELKKTMTGLYSQCMKGRTMYVIPF
EEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEE
SMGPVGSPISKNGIEITDSEYVVCNMHIMTRVGTRVLEALGTDGEFVPCLHSVGKPLGPG
CCCCCCCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCC
VTDAGQWPCADMERKYISHFPEERLVWSFGSGYGGNALLGKKCFALRIASVLARDEGWLA
CCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHH
EHMLILKITNPAGKTKYIGAAFPSACGKTNLAMMIPTLPGWKVETVGDDIAWMKFGKDGR
HCEEEEEEECCCCCCEEECCCCCHHCCCCCEEEEEECCCCCEEEECCCCEEEEEECCCCC
LYAINPEAGFFGVAPGTSDFSNKNAMDSIKENAIFTNCGLTEDGDVWWEGIGYPAKGTII
EEEECCCCCEEECCCCCCCCCCCHHHHHHHHCCEEEECCCCCCCCEEEECCCCCCCCCEE
DWHGVSRPAPTRDKSPKGEEIAHPNARFTAPARQCPAIASNWEDPEGVPIDAFLFGGRRP
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHCCCCCCCCCEEEEEEECCCCC
STVPLVHQARDWNHGVFLGSIIGSEVTAAVISDQVGQIRRDPFAMLPFCGYHMADYFSHW
CCCCHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
IKLGSQARAENLPKIFCVNWFRKDAEGNFLWPGYGDNSRVLAWIFDRCDGVDNAVETAIG
HHCCCHHHHCCCCEEEEEHHHHHCCCCCEECCCCCCCCCEEEEEEHHCCCCCHHHHHHHC
WMPKEGALNTEGLNVSTQAVKELLSVDIAGWKKEIKDIRENHYPKFGARLPQQLRDALEV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LEARINGSEGAACTRDMC
HHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876